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TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

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Agricultural Sciences in China 2011年第11期10卷 1732-1738页

作者： ZHANG Li-rong YANG Wen-xiang liu da-qun College of Plant Protection Biological Control Center of Plant Diseases and Pests of Hebei Province Agricultural University of HebeiBaoding 071001 P.R.China

Virus-induced gene silencing （VIGS） offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus （BSMV） VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RT- PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.

关键词： disease resistance barley stripe mosaic virus (BSMV) virus-induced gene silencing (VIGS) Lr24 RAR1

小麦TaPR1基因启动子分析及其调控因子的筛选

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农业生物技术学报 2024年第6期32卷 1227-1235页

作者：申松松王丽珊冯燕崔钟池刘园霞刘大群王海燕河北农业大学植物保护学院/河北省农作物病虫害生物防治技术创新中心

病程相关蛋白1 (pathogenesis-related protein 1, PR1)是植物重要的抗逆防御蛋白，小麦(Triticum aestivum) TaPR1基因受小麦叶锈菌(Puccinia triticina, Pt)诱导表达，参与小麦对叶锈病的防御反应。本课题组前期利用基因克隆方法成功... 详细信息

病程相关蛋白1 (pathogenesis-related protein 1, PR1)是植物重要的抗逆防御蛋白，小麦(Triticum aestivum) TaPR1基因受小麦叶锈菌(Puccinia triticina, Pt)诱导表达，参与小麦对叶锈病的防御反应。本课题组前期利用基因克隆方法成功获得TaPR1启动子序列，命名为p TaPR1，其中900 bp序列(p TaPR1900)具有较强的活性。本研究进一步对p TaPR1的序列组成和可能的调控因子进行分析。根据PLACE和PlantCARE数据库初步分析，p TaPR1序列中包含核心元件TATA-box、CAAT-box及大量光反应元件，还包含脱落酸应答元件和响应低温诱导的MYB结合位点。利用酵母单杂交技术(yeast one-hybrid, Y1H)筛选，共获得12个可能与p TaPR1900结合的转录因子；利用Y1H回复验证，初步证实U-box E3泛素连接酶、花粉蛋白、甘氨酸A3蛋白和重金属伴生异戊二烯基植物蛋白与p TaPR1900互作。本研究为深入探讨TaPR1转录调控机制提供理论参考。

关键词：小麦病程相关蛋白1 (TaPR1) 启动子顺式作用元件酵母单杂交(Y1H) 转录因子

Isolation and Characterization of NBS-LRR Class Resistance Homologous Gene from Wheat

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Agricultural Sciences in China 2011年第8期10卷 1151-1158页

作者： ZHANG Nan WANG Shen WANG Hai-yan liu da-qun College of Plant Protection/Biological Control Center of Plant Disease and Plant Pests of Hebei Province Agricultural University of HebeiBaoding 071001P.R.China

One resistance gene analog fragment named RGA-CIN14 was isolated from TcLr19 wheat,which contains kinase-2,kinase-3a,and the GLPL motif of the NBS-spanning region,using degenerated primers according to the nucleotide binding site （NBS） conserved *** on the RGA-CIN14,a full-length cDNA,CIN14,which was 2 987 bp encoding 880 amino acids,was obtained by using the method of the rapid amplification cDNA ends （RACE）.Bioinformatics analysis showed that the deduced amino acids of CIN14 protein consisted of a NB-ARC conserved domain and many leucine-rich repeats （LRR） *** phylogenetic tree analysis indicated a considerable identity of the protein encoded by CIN14 with that of wheat leaf rust resistance gene Lr1,but a lower similarity with *** expression profile of the CIN14 gene detected by semi-quantitative RT-PCR showed that the CIN14 gene was not induced by Puccinia triticina and it was a constitutive gene with low abundance in the wheat leaf *** resistance homology sequence was successfully obtained,which provides the shortcut for cloning of the resistance gene in TcLr19 wheat.

关键词： wheat leaf rust resistance gene NBS-LRR resistance gene analogs (RGAs) rapid amplification cDNA end (RACE) RT-PCR

Identification of leaf rust resistance genes in common wheat varieties from China and foreign countries

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Journal of Integrative Agriculture 2021年第5期20卷 1302-1313页

作者： liu Yuan Takele Weldu GEBREWAHID ZHANG Pei-pei LI Zai-feng liu da-qun College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China College of Agriculture Aksum UniversityShire-Indaslassie 314Ethiopia

Wheat leaf rust,triggered by Puccinia triticina Eriks(Pt),is among the most important diseases of wheat *** resistant varieties against leaf rust is the most effective,environmentally-friendly and economic way to control the *** the present study,66 wheat varieties form China and foreign countries were tested with 17 Pt races for gene postulation during the seedling stage in the *** the varieties were also planted to identify slow rusting responses to leaf rust at the adult plant stage in Baoding and Zhoukou field trials during the 2016/2017 to 2017/2018 cropping ***,12 closely linked molecular markers to known leaf rust resistance(Lr)genes were used for assessing all the *** results of both gene postulation and molecular marker identification showed that a total of eight Lr genes,Lr1,Lr10,Lr17,Lr20,Lr26,Lr34,Lr37 and Lr46,either singly or in combination were detected in 32 *** Lr genes were not identified in the remaining 34 *** varieties were found to have slow rusting *** resistance sources identified in this study can be used as resources for resistance against leaf rust in wheat breeding programs in China and the respective foreign countries.

关键词： wheat gene postulation molecular marker Lr gene

Mapping of QTL conferring leaf rust resistance in Chinese wheat lines W014204 and Fuyu 3 at adult plant stage

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Journal of Integrative Agriculture 2016年第1期15卷 18-28页

作者： QI Ai-yong ZHANG Pei-pei ZHOU Yue YAO Zhan-jun LI Zai-feng liu da-qun Department of Plant Pathology College of Plant Protection Agricultural University of Hebei Department of Biochemistry Baoding University College of Agronomy Agricultural University of Hebei

Wheat leaf rust is a destructive foliar disease of common wheat （Triticum aestivum L.） worldwide. The most effective, economical s to control the disease is growing resistant cultivars with adult plant resistance （APR）. The Chinese wheat tines W014204 and Fuyu 3 showed high leaf rust resistance in the field. To identify leaf rust APR genes in the two lines, two mapping populations with 215 and 163 F2：3 lines from the crosses W014204/Zhengzhou 5389 and Fuyu 3/Zhengzhou 5389, respectively, were phenotyped for leaf rust severities during the 2010-2011, 2011-2012 and 2012-2013 cropping seasons in the field at Baoding, Hebei Province, China. A total of 1 215 SSR markers were used to identify the quantitative trait loci （QTLs） for leaf rust APR in the two populations. In the W014204/Zhengzhou 5389 population, three QTLs were detected and designated as ***-lBL.1, ***-2BS.1 and ***-7DS, and explained 2.9-8.4, 11.5-38.3 and 8.5-44.5% of the phenotypic variance, respectively; all the resistance alleles at these loci were derived from W014204. In the Fuyu 3/Zhengzhou 5389 population, three QTLs, ***-lBL.2, ***-2BS.2 and ***-7BL, explained 12.0-19.2, 22.3-38.9 and 4.1-4.3% of the phenotypic variance, respectively, and all resistance alleles were contributed by Fuyu 3. Based on chromosome positions of closely linked markers, both ***-lBL. 1 and ***-lBL.2 are Lr46, and ***-7DS is Lr34. ***-7BL was mapped on chromosome 7BL near to Lr68 and they are likely the same gene. Based on chromosome positions, pedigree and field reactions, the two 2BS QTLs are different from all the known APR genes and are likely to be newAPR QTL for leaf rust. These QTLs and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.

关键词： Triticum aestivum Puccinia triticina APR SSR marker

Systemic acquired resistance, NPR1, and pathogenesis-related genes in wheat and barley

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Journal of Integrative Agriculture 2018年第11期17卷 2468-2477页

作者： WANG Xiao-dong BI Wei-shuai GAO Jing YU Xiu-mei WANG Hai-yan liu da-qun College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of Hebei Hebei Agricultural University Baoding 071000 P.R.China College of Life Sciences Hebei Agricultural University Baoding 071000 P.R.China Graduate School of Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China

In Arabidopsis, systemic acquired resistance （SAR） is established beyond the initial infection by a pathogen or is directly induced by treatment with salicylic acid （SA） or its functional analogs, 2,6-dichloroisonicotinic acid （INA） and benzothiadiazole （BTH）. NPR1 protein is considered the master regulator of SAR in both SA signal sensing and transduction. In wheat （Triticum aesfivum） and barley （Hordeum vulgare）, both pathogen infection and BTH treatment can induce broad-spectrum resistance to various diseases, including powdery mildew, leaf rust, Fusarium head blight, etc. However, three different types of SAR-like responses including acquired resistance （AR）, systemic immunity （SI）, and BTH-induced resistance （BIR） seem to be achieved by activating different gene pathways. Recent research on wheat and barley NPR1 homologs in AR and SI has provided the initial clue for understanding the mechanism of SAR in these two plant species. In this review, the specific features ofAR, Si, and BIR in wheat and barley were summarized and compared with that of SAR in model plants of Arabidopsis and rice. Research updates on downstream genes of SAR, including pathogenesis-related （PR） and BTH-induced genes, were highlighted.

关键词： systemic acquired resistance NPR1 pathogenesis-related genes wheat barley

Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes

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Journal of Integrative Agriculture 2019年第5期18卷 1014-1023页

作者： ZHANG Pei-pei Takele Weldu Gebrewahid ZHOU Yue LI Qing-luo LI Zai-feng liu da-qun Department of Plant Pathology College of Plant Protection Hebei Agricultural University College of Agriculture Aksum University-Shire Campus College of Biochemistry and Environmental Engineering Baoding University

Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat *** most economic,safe and effective way to control the disease is growing resistant *** the present study,a total of 46 wheat landraces and 34 wheat lines with known Lr(leaf rust resistance)genes were inoculated with 16Pt pathotypes for postulating seedling resistance gene(s)in the *** cultivars and five wheat differential lines with adult plant resistance(APR)genes(Lr12,Lr22b,Lr34,Lr35 and Lr37)were also evaluated for identification of slow rusting resistance in the field trials in Baoding,Hebei Province of China in the 2014–2015 and 2015–2016 cropping ***,10 functional molecular markers closely linked to 10 known Lr genes were used to detect all the wheat *** showed that most of the landraces were susceptible to most of the Pt pathotypes at seedling ***,Lr1 was detected only in *** field experimental test of the two environments showed that 38 landraces showed slow rusting *** cultivars possessed Lr34 but none of the landraces contained Lr37 and *** genes namely,Lr9,Lr19,Lr24,Lr28,Lr29,Lr47,Lr51 and Lr53 were effective at the whole plant ***18,Lr36 and Lr45 had lost resistance to part of pathotypes at the seedling stage but showed high resistance at the adult plant ***34 as a slowing rusting gene showed good resistance in the *** race-specific APR genes Lr12,Lr13,Lr35 and Lr37 conferred good resistance in the field *** race-specific genes,Lr2b,Lr2c,Lr11,Lr16,Lr26,Lr33 and LrB had lost *** 38 landraces showed slow rusting resistance to wheat leaf rust can be used as resistance resources for wheat resistance breeding in China.

关键词： Triticum aestivum L. wheat leaf rust gene postulation adult plant resistance

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

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Journal of Integrative Agriculture 2013年第9期12卷 1580-1588页

作者： SHI Li-hong ZHANG Na HU Ya-ya WEI Xue-jun YANG Wen-xiang liu da-qun Department of Plant Pathology College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of HebeiAgricultural University of Hebei

To detect the leaf rust resistance genes in the 7 Chinese spring wheat clultivars Shenmian 99025, Shenmia 99042, Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 1167 and Shenmian 962, Thatcher, Thatcher backgrounded near-isogenic lines and 15 pathotypes of P. triticina were used for gene postulate at the seedling stage, and 9 of the 15 pathotypes were used in the field tests. Molecular markers closely linked to, or co-segregated with resistance genes Lrl, Lr9, LrlO, Lrl9, Lr20, Lr21, Lr24, Lr26, Lr28, Lr29, Lr32, Lr34, Lr35, Lr37, Lr38, and Lr47 were screened to assist detection of the resistance genes. As results, 4 known resistance genes, including Lrl, Lr9, Lr26, and Lr34, and other unknown resistance genes were postulated singly or in combination in the tested cultivars. Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 962, Shenmian 1167, and Shenmian 99042 are potentially useful for wheat production and breeding programs. The result suggested that combining gene postulation, molecular markers and pedigrees is effective and more accuracy method to know the resistance genes in cultivars.

关键词： gene postulation molecular marker Triticum aestivum Puccinia triticina resistance gene

Molecular mapping of leaf rust resistance genes in the wheat line Yu 356-9

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Journal of Integrative Agriculture 2015年第7期14卷 1223-1228页

作者： HAN liu-sha LI Zai-feng WANG Jia-zhen SHI Ling-zhi ZHU Lin LI Xing liu da-qun Syed J A Shah College of Plant Protection Agricultural University of Hebei Nuclear Institute for Food and Agricuture

The Chinese wheat line Yu 356-9 exhibits a high level of resistance to leaf rust. In order to decipher the genetic base of resistance in Yu 356-9, gene postulation, inheritance analyses, and chromosome linkage mapping were carried out. Gene postulation completed using 15 leaf rust pathotypes and 36 isogenic lines indicated that Yu 356-9 was resistant to all pathotypes tested. F1 and F2 plants from the cross Yu 356-9 （resistant）/Zhengzhou 5389 （susceptible） were tested with leaf rust pathotype ＂FHNQ＂ in the greenhouse. Results indicated a 3：1 segregation ratio, indicative of the presence of a single dominant leaf rust resistance gene in Yu 356-9 which was temporarily designated as LrYu. Bulk segregant analysis and molecular marker assays were used to map LrYu. Five simple sequence repeat （SSR） markers on chromosome 2BS were found closely linked to LrYu. Among these markers, Xwmc770 is the most closely linked, with a genetic distance of 5.7 cM.

关键词： wheat leaf rust resistance gene inheritance analyses molecular mapping