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蚯蚓粪在农业方面的应用研究进展

山西大同大学学报（自然科学版） 2024年第1期40卷 13-16页

作者：李彩琴刘荔贞王琴姚宇郭靖荣山西大同大学化学与化工学院山西大同037009

在我国提倡实施符合经济和环境发展的绿色农业措施下,蚯蚓粪这种绿色、高效、含有丰富营养物质的有机生物肥料受到越来越多人的关注。本文从作物生长、修复土壤、抑制作物病虫害、缓解作物连作障碍等方面综述了蚯蚓粪在农业方面的应用,... 详细信息

在我国提倡实施符合经济和环境发展的绿色农业措施下,蚯蚓粪这种绿色、高效、含有丰富营养物质的有机生物肥料受到越来越多人的关注。本文从作物生长、修复土壤、抑制作物病虫害、缓解作物连作障碍等方面综述了蚯蚓粪在农业方面的应用,展望了蚯蚓粪的应用领域和效果值得深入研究。

关键词：蚯蚓粪农业土壤

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子宫静脉内平滑肌瘤1例并文献复习

妇儿健康导刊 2024年第2期3卷 45-48页

作者：高佳欣李丽孙聪聪刘利贞张英姿滨州医学院附属医院妇产科山东滨州256600

子宫静脉内平滑肌瘤(IVL)是一种良性肿瘤,但可沿血管生长,表现出恶性生物学行为,临床上较为少见,严重者可累及多个器官,临床表现多样化。IVL发生率较低,且无特异性诊断方法,易发生漏诊、误诊情况。手术是本病首选的治疗方法,术前应充分... 详细信息

子宫静脉内平滑肌瘤(IVL)是一种良性肿瘤,但可沿血管生长,表现出恶性生物学行为,临床上较为少见,严重者可累及多个器官,临床表现多样化。IVL发生率较低,且无特异性诊断方法,易发生漏诊、误诊情况。手术是本病首选的治疗方法,术前应充分评估患者情况、了解疾病受累情况、患者生育要求等,提供个性化手术方式。术后应定期随访,预防疾病复发。本文报道1例IVL患者的诊疗经过,总结IVL疾病特点,提高对IVL的认识。

关键词：子宫静脉内平滑肌瘤疾病受累情况疾病特点

Revealing Functional Significance of Interleukin-2 Glycopro-teoforms Enabled by Expressed Serine Ligation

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Chinese Journal of Chemistry 2022年第7期40卷 787-793页

作者： Jie Zhao Jiazhi liu Xinnan liu Qi Cao Hongbo Zhao lizhen liu Farong Ye Can Wang Hong Shao Dongxiang Xue Houchao Tao Bin Li Biao Yu Ping Wang Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs School of Chemistry and Chemical EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China Shanghai Institute of Immunology Department of Immunology and MicrobiologyShanghai Jiao Tong University School of Medicine280 S.Chongqing RoadShanghai 200025China Obstetrics and Gynecology Hospital of Fudan University Shanghai 200011China Shanghai Institute for Food and Drug Control NMPA Key Laboratory for Quality Control of Therapeutic Monoclonal AntibodiesShanghai 201203China iHuman Institute ShanghaiTech UniversityHaike Road 230Shanghai 200032China State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of Sciences345 Lingling RoadShanghai 200032

Naturally occurring interleukin-2(IL-2)is a pleiotropic glycoprotein that regulates immune responses by controlling the differentia-tion and homeostasis of T ***-glycosylated IL-2 has been used in clinical settings for three ***,the function of the O-glycan of native IL-2 remains ***,to stress this issue,we report a highly efficient semi-synthesis of homogeneous glycosylated IL-2 with various glycoproteoforms on a multi-milligram *** glycopeptide fragment was prepared by chemical synthesis and then merged with recombinant fragment via a serine ligation to generate the desired glycoprotein in a single *** evaluation of the homogenous glycoprotein library reveals that the activity of IL-2 in activating individual T cell subset is glycan dependent,thus highlighting the possibility of further improving current clinical medicine.

关键词： Oligosaccharide Glycoprotein Serine ligation Interleukin-2 Glycopeptides

BOPPPS联合互联网+在骨科手术临床护理教学中的应用

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中国继续医学教育 2024年第11期16卷 93-97页

作者：陈燕珠黄馥桦陈伟璇陈育春刘丽贞汕头大学医学院第二附属医院手术室广东汕头515041 汕头大学医学院第二附属医院骨外科广东汕头515041 汕头大学医学院第二附属医院泌尿外科广东汕头515041

目的探讨BOPPPS[导入(bridge)、目标(objective)、预评价(pre-assessment)、参与式学习(participation)、后评价(post-assessment)、总结(summary)]联合互联网+在骨科手术临床护理教学的应用。方法选取汕头大学医学院2019年7月—2023年5月四年制护理学专业70名学生为研究对象。将2019-2021年度34名学生为对照组,2021-2023年度的36名学生为干预组。2组学生均由同一名骨科手术组医生进行手术演示,由同一名高年资主管护师进行临床带教。干预组采用BOPPPS联合互联网+教学,对照组采用传统教学模式,教学结束后分别对2组学生理论知识和临床技能进行评估,并采用问卷调查的方法,收集授课教师和2组学生对教学的反馈。结果干预组理论知识成绩、临床技能整体评分均高于对照组,差异有统计学意义(P <0.05)。干预组教学满意度评分高于对照组,差异有统计学意义(P <0.05)。干预组教学效果评价均优于对照组,差异有统计学意义(P <0.05)。干预组学生对BOPPPS联合互联网+教学方法满意度100%。干预组临床思维能力评分均优于对照组,差异有统计学意义(P <0.05)。结论BOPPPS联合互联网+有助于提高学生理论和技能成绩、提高学生临床思维能力和增强教师教学技能,达到教学相长。

关键词： BOPPPS 互联网+ 骨科临床教学手术室临床护理

Establishment of a pseudovirus neutralization assay based on SARS-CoV-2 S protein incorporated into lentiviral particles

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Biosafety and Health 2022年第1期4卷 38-44页

作者： Sheng Wang lizhen liu Can Wang Ziqiang Wang Xuhua Duan Gang Chen Hu Zhou Hong Shao Department of Biochemical Drugs and Biological Products Shanghai Institute for Food and Drug ControlShanghai 201203China NMPA Key Laboratory for Quality Control of Therapeutic Monoclonal Antibodies Shanghai Institute for Food and Drug ControlShanghai 201203China School of Pharmacy Fudan UniversityShanghai 201203China Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China Department of Analytical Chemistry Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

The coronavirus disease 2019(COVID‐19)is still causing a wide range of infections and deaths due to the high variability of the severe acute respiratory syndrome coronavirus 2(SARS‐CoV‐2).Therefore,it is necessary to establish a reliable and convenient pseudovirus‐based neutralization assay to develop drug targeted variants of SARS‐CoV‐*** on the HIV‐1 backbone,we generated a high titer luciferase(Luc)‐expressing pseudovirus packaging *** dominant S mutant substitution pseudovirus were also established and identified compared to wide type in hACE2‐overexpressingHEK‐293T cells(293T‐ACE2 cells).Compared to serine protease inhibitor camostat mesylate,the cysteine protease inhibitor E‐64d could significantly block all SARS‐CoV‐2 mutant S pseudovirus infection in 293T‐ACE2 ***,the neutralization ability of two antibodies targeted receptor‐binding domain(RBD)of SARS‐CoV‐2 spike protein(S)was evaluated,which showeddifferent inhibition dose–effect curves among four types of S ***,we developed a pseudovirus‐based neutralization assay for SARS‐CoV‐2,which would be readily adapted to SARS‐CoV‐2 variants for evaluating antibodies.

关键词： COVID‐19 SARS‐CoV‐2 variants Pseudovirus Neutralizing antibody RBD

A Novel Dual Pointer Approach for Entity Mention Extraction

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Chinese Journal of Electronics 2021年第1期30卷 127-133页

作者： liu Jie PANG Yihe ZHANG Kai liu lizhen YU Zhengtao Department of Information Engineering Capital Normal University Research Center for Language Intelligence Capital Normal University Department of Information Engineering and Automation Kunming University of Science and Technology

The named entity extraction task aims to extract entity mentions from the unstructured text,including names of people, places, institutions and so on. It plays an important role in many Natural language processing(NLP) tasks, such as knowledge bases construction, automatic question answering system and information extraction. Most of the existing entity extraction studies are based on the long text data, which are easier to annotate due to the sufficient contextual information. Extracting entities from short texts such as search queries, conversations is still a challenging *** paper proposes a dual pointer approach for entity mention extraction, it extracts one entities by two position pointers of the input sentence. The end-to-end deep neural networks model based on the proposed approach can extract the entities by serially generating the dual *** evaluation results on the Chinese public dataset show that the model achieves the state-of-the-art results over the baseline models.

关键词： Dual pointer approach Entity extraction Deep neural networks Natural language processing

A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E

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Journal of Integrative Agriculture 2024年第4期23卷 1354-1368页

作者： Huanhuan Lü Pinpin Ji Siyu liu Ziwei Zhang Lei Wang Yani Sun Baoyuan liu lizhen Wang Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F University/Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic TechnologyMinistry of Agriculture and Rural AffairsYangling 712100China

Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry *** glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some *** has the advantages of small size and easy genetic engineering and has been a promising diagnostic ***,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE *** the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian ***,two nanobodies against PRV-gE were screened from the immunized camel by phage display ***,two nanobody-HRP fusion proteins were expressed with HEK293T *** PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE *** optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:*** cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,*** detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV ***,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were *** bELISA could be used for monitoring and eradicating PR.

关键词： nanobody nanobody-HRP blocking ELISA PRV-gE antibody

继发急性髓系白血病异基因造血干细胞移植疗效及预后影响因素分析

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中华血液学杂志 2024年第1期45卷 41-47页

作者：袁晓琳吴一波宋晓露陈怡陆滢来晓瑜施继敏刘丽珍赵妍敏余建杨露欣蓝建平蔡真黄河罗依浙江大学医学院附属第一医院骨髓移植中心、良渚实验室、浙江大学血液学研究所、浙江省干细胞与细胞免疫治疗工程实验室杭州310003 浙江省人民医院杭州310014 温州医科大学附属第一医院温州325035 宁波大学附属人民医院宁波315000

目的探讨成人继发急性髓系白血病(AML)接受异基因造血干细胞移植(allo-HSCT)的疗效及预后影响因素。方法多中心回顾性临床研究。纳入2014年1月至2022年11月因继发AML在浙江省造血干细胞移植协作组4个中心接受allo-HSCT的18岁以上的成人... 详细信息

目的探讨成人继发急性髓系白血病(AML)接受异基因造血干细胞移植(allo-HSCT)的疗效及预后影响因素。方法多中心回顾性临床研究。纳入2014年1月至2022年11月因继发AML在浙江省造血干细胞移植协作组4个中心接受allo-HSCT的18岁以上的成人患者,并进行疗效及预后影响因素分析。结果共纳入95例患者,其中66例(69.5%)为骨髓增生异常综合征(MDS)转化AML(MDS-AML),4例(4.2%)为MDS/骨髓增殖性肿瘤(MPN)转化AML(MDS/MPN-AML),25例(26.3%)为治疗相关AML(tAML)。所有患者的3年累积复发率(CIR)、无白血病生存(LFS)率和总生存(OS)率分别为18.6%(95%CI 10.2%~27.0%)、70.6%(95%CI 60.8%~80.4%)和73.3%(95%CI 63.9%~82.7%)。M-AML(包括MDS-AML、MDS/MPN-AML)组和tAML组的3年CIR分别为20.0%和16.4%(P=0.430);3年LFS率分别为68.3%和75.4%(P=0.176);3年OS率分别为69.7%和75.4%(P=0.233),两组间差异均无统计学意义。TP53突变组和无TP53突变组的3年CIR分别为60.0%和13.7%(P=0.003),3年LFS率分别为20.0%和76.5%(P=0.002),3年OS率分别为40.0%和77.6%(P=0.002)。根据2022欧洲白血病网(ELN2022)危险分层,低危、中危和高危3组患者的3年CIR分别为8.3%、17.8%和22.6%(P=0.639),3年LFS率分别为91.7%、69.5%和65.6%(P=0.268),3年OS率分别为91.7%、71.4%和70.1%(P=0.314)。多因素分析表明,移植时疾病未缓解和伴有TP53突变是影响患者CIR、LFS和OS的独立危险因素。结论M-AML组(MDS-AML、MDS/MPN-AML)与tAML组患者allo-HSCT预后相近。移植时疾病未缓解和伴有TP53突变是不良预后因素。ELN2022危险分层对继发AML患者allo-HSCT预后的预测价值有限。

关键词：白血病髓系急性继发白血病造血干细胞移植生存复发

Multi-Domain Collaborative Recommendation with Feature Selection

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China Communications 2017年第8期14卷 137-148页

作者： lizhen liu Junjun Cui Wei Song Hanshi Wang Information and Engineering College Capital Normal UniversityBeijing 100048.China

Collaborative f iltering, as one of the most popular techniques, plays an important role in recommendation systems. However,when the user-item rating matrix is sparse,its performance will be degenerate. Recently,domain-specific recommendation approaches have been developed to address this *** basic idea is to partition the users and items into overlapping domains, and then perform recommendation in each domain independently. Here, a domain means a group of users having similar preference to a group of products. However, these domain-specific methods consisting of two sequential steps ignore the mutual benefi t of domain segmentation and recommendation. Hence, a unified framework is presented to simultaneously realize recommendation and make use of the domain information underlying the rating matrix in this paper. Based on matrix factorization,the proposed model learns both user preferences of multiple domains and preference selection vectors to select relevant features for each group of products. Besides, local context information is utilized from the user-item rating matrix to enhance the new *** results on two widely used datasets, e.g., Ciao and Epinions, demonstrate the effectiveness of our proposed model.

关键词：协同过滤特征选择用户偏好上下文信息矩阵分解产品选择推荐系统相关特征