Revealing Functional Significance of Interleukin-2 Glycopro-teoforms Enabled by Expressed Serine Ligation
作者机构：Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China Shanghai Institute of ImmunologyDepartment of Immunology and MicrobiologyShanghai Jiao Tong University School of Medicine280 S.Chongqing RoadShanghai 200025China Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200011China Shanghai Institute for Food and Drug ControlNMPA Key Laboratory for Quality Control of Therapeutic Monoclonal AntibodiesShanghai 201203China iHuman InstituteShanghaiTech UniversityHaike Road 230Shanghai 200032China State Key Laboratory of Bioorganic and Natural Products ChemistryCenter for Excellence in Molecular SynthesisShanghai Institute of Organic ChemistryChinese Academy of Sciences345 Lingling RoadShanghai 200032
出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)
年 卷 期：2022年第40卷第7期
页 面：787-793页
核心收录：
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：supported by the National Natural Science Foundation of China(22077080,21907064 and 22107068) Special Projects of the Central Government in Guidance of Local Science and Technology Development(2021Szvup077)
主 题：Oligosaccharide Glycoprotein Serine ligation Interleukin-2 Glycopeptides
摘 要：Naturally occurring interleukin-2(IL-2)is a pleiotropic glycoprotein that regulates immune responses by controlling the differentia-tion and homeostasis of T ***-glycosylated IL-2 has been used in clinical settings for three ***,the function of the O-glycan of native IL-2 remains ***,to stress this issue,we report a highly efficient semi-synthesis of homogeneous glycosylated IL-2 with various glycoproteoforms on a multi-milligram *** glycopeptide fragment was prepared by chemical synthesis and then merged with recombinant fragment via a serine ligation to generate the desired glycoprotein in a single *** evaluation of the homogenous glycoprotein library reveals that the activity of IL-2 in activating individual T cell subset is glycan dependent,thus highlighting the possibility of further improving current clinical medicine.
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