检索结果-南通市图书馆

Divergent Synthesis of Core m1,Core m2 and Core m3 O-Mannosyi glycopeptides via a Chemoenzymatic Approach

Chinese Journal of Chemistry 2022年第13期40卷 1571-1577,I0002页

作者： Tianlu Li Youqin Zhang Tong Li Haoru Zhuang Fengshan Wang Ning Wang Richard R.Schmidt Peng Peng National Glycoengineering Research Center Shandong Key Laboratory of Carbohydrate Chemistry and GlycobiologyNMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate Based MedicineShandong UniversityQingdaoShandong 266237China Shenzhen Research Institute of Shandong University ShenzhenGuangdong 518057China Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxiJiangsu214122China Department of Chemistry University of KonstanzKonstanz D-78457Germany

O-Mannosylation plays a vital role in the regulation of a variety range of biological processes,for instance,brain and muscle ***,the precise function remains largely unknown due to its innate *** this regard,it is still welcome to develop efficient methods to access diverse structurally-defined *** this study,a diversity-oriented assembly of O-mannosylα-dystroglycan(α-DG)glycopeptides has been achieved via a chemoenzymatic *** strategy features(i)gram scale divergent synthesis of core m1,core m2 and core m3 mannosylated amino acids from judiciously designed protecting group strategies and chemical glycosidation;(i)efficient glycopeptide assembly via the optimized microwave-assisted solid phase peptide synthesis(SPpS);and(ii)enzymatic elaboration of the core glycan structures to install galactosyl and sialyl-galactosyl *** efficiency and flexibility of this chemoenzymatic approach was demonstrated with the construction of 12 glycopeptides with different core m1,core m2 and core m3 mannosyl glycans,including a core m2 glycopeptide bearing a heptasaccharide for the first time.

关键词： glycopeptides oligosaccharides Regioselectivity Enzyme catalysis Divergent synthesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hydrophilic adamantane derivatives engineered β-cyclodextrin-based self-assembly materials for highly efficient enrichment of glycopeptides

引用

Chinese Chemical Letters 2023年第3期34卷 283-287页

作者： Ping Li Zheng Li Dawei Zhang Qiong Jia College of Chemistry Jilin UniversityChangchun 130012China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life SciencesJilin UniversityChangchun 130012China

β-Cyclodextrin(β-CD) based materials have attracted great attention in the separation of hydrophilic glycopeptides due to the abundant hydroxyl groups in its exterior. However, the current materials based on β-CD generally has complex synthesis process and harsh experimental conditions, on the other hand,the interior cavity of β-CD is hydrophobic and is harmful to capture glycopeptides. Herein, a novel hydrophilic material based on β-CD was engineered via a self-assembly process utilizing L-cysteine(L-Cys)or glutathione(GSH) derived adamantane for highly efficient glycopeptide enrichment. It is the first attempt to make use of the hydrophobic interior cavity of β-CD for hydrophilic glycopeptide capture. Taking advantages of strong hydrophilicity and superparamagnetism, the as-prepared materials possess low detection limit, high selectively, and excellent reusability when employed to glycopeptide enrichment. In addition, the feasibility of the hydrophilic material based on β-CD was verified by enriching glycopeptides from human serum and saliva samples. This study provides a heuristic strategy for the application of β-CD-based self-assembly materials in the enrichment of glycopeptides. Importantly, this strategy certified a possible that the change of glycopeptide enrichment sites through host-guest interaction between β-CD and adamantane derivatives with different functional groups.

关键词： β-Cyclodextrin Hydrophilic Self-assembly Enrichment glycopeptides

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Specific enrichment of urinary exosomes and exosomal glycopeptides by coefficient affinity of integrated L-cysteine and titania

引用

Chinese Chemical Letters 2023年第2期34卷 254-258页

作者： Yijie Chen Haolin Chen Chenjie Yang Yonglei Wu Chunhui Deng Nianrong Sun Department of Chemistry Institute of Metabolism&Integrate Biology(IMIB)Fudan UniversityShanghai 200433China Department of Gastroenterology and Hepatology Zhongshan HospitalFudan UniversityShanghai 200032China

Exosome and inclusive cargoes have manifested significant function in different biological events. In particular, glycopeptides in exosome are closely associated with occurrence and development of various diseases. Developing advanced tools is highly desired to enrich glycopeptides from exosomes, and enrich exosomes from complex biological samples as well. In this work, integration of L-cysteine and titania onto the surface of magnetic nanoparticles is designed to realize the coefficient affinity towards exosomes and inclusive glycopeptides. Benefiting from the synergistic affinity, we separate exosomes from human urine concentrate directly, which was proved by the detection of three typical antigen markers of exosomes. Furthermore, hardly any exosomes remained on materials after ultrasonication, which confirmed the good capture performance of Fe_(3)O_(4)@TiO_(2)@L-Cys and high release effect of direct ***, 146 glycopeptides corresponding to 77 glycoproteins were successfully identified from captured exosomes. These satisfactory results will inspire more efforts to be devoted to this field and will be extremely helpful to in-depth information excavation of biological markers, especially disease-related ones, through exosomes and inclusive glycopeptides.

关键词： glycopeptides Enrichment Nanomaterials Exosomes Urine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Application of magnetic solid phase extraction in separation and enrichment of glycoproteins and glycopeptides

引用

Chinese Chemical Letters 2021年第9期32卷 2629-2636页

作者： He Qi Liyan Jiang Qiong Jia College of Chemistry Jilin UniversityChangchun 130012China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun 130012China

The analysis of endogenous glycoproteins and glycopeptides in human body fluids is of great importance for screening and discovering disease biomarkers with clinical ***,the presence of interfering substances makes the direct quantitative detection of low-abundance glycoproteins and glycopeptides in human body fluids one of the great challenges in analytical *** solid phase extraction(MSPE)has the advantages of easy preparation,low cost and good magnetic *** adsorbents are the core of MSPE technology,and magnetic adsorbents based on different functional materials are widely used in the quantitative analysis of glycoproteins and glycopeptides in human body fluids,making it possible to analyze glycoproteins and glycopeptides with low abundance as well as multiple types,which provides a technical platform for screening and evaluating glycoproteins and glycopeptides in body fluids as disease *** this paper,we focus on the recent advances in the application of MSPE technology and magnetic adsorbents for the separation and enrichment of glycoproteins and glycopeptides in human body fluids,and the future trends and application prospects in this field are also presented.

关键词： Glycoproteins glycopeptides Magnetic solid phase extraction(MSPE) Separation Enrichment Human body fluids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Facile preparation of hydrophilic glutathione modified magnetic nanomaterials for specific enrichment of glycopeptides

引用

Chinese Chemical Letters 2019年第12期30卷 2181-2185页

作者： He Qi Zheng Li Haijiao Zheng Li Fu Qiong Jia College of Chemistry Jilin UniversityChangchun 130012China The Second Hospital of Jilin University Changchun 130041China Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun 130012China

Investigations of glycosylated proteins or peptides and their related biological pathways provide new possibilities for illuminating the physiological and pathological mechanisms of glycosylation ***,open-ended and in-depth analysis of glycoproteomics is usually subjected to the low-abundance of glycopeptides,heterogeneous glycans,and a variety of interference *** order to alleviate the influence of these obstacles,effective preconcentration of glycopeptides are ***,we employed a hydrophilic interaction liquid chromatography(HILIC)-based method to universally capture *** modified magnetic nanoparticles(Fe304@AuGSH) were synthesized through a simple process and exploited to enrich glycopeptides from complex *** prepared materials showed excellent ability to trap glycopeptides from standard glycoproteins digests,low detection limit(10 fmol/p L),and good selectivity(HRP:BSA=1:100).These results indicated that glutathione-based magnetic nanoparticles synthesized in this work had great potential for glycopeptides enrichment.

关键词： Hydrophilic Glutathione Magnetic Enrichment glycopeptides

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Facile Synthesis of Boronic Acid-Functionalized Magnetic Mesoporous Silica Nanocomposites for Highly Specific Enrichment of glycopeptides

引用

Chinese Journal of Chemistry 2011年第4期29卷 835-839页

作者：张怀远姚国平邓春晖陆豪杰杨芃原 Department of Chemistry and Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

In the work, aminophenylboronic acid （APB）-functionalized magnetic mesoporous silica, which holds the attractive features of high magnetic responsivity and large surface area, was developed to enrich glycopeptides. At first, magnetic mesoporous silica nanocomposites were prepared. And then, the nanocomposites were functioned with glycidoxypropyltrimethoxysilane （GLYMO） for boronic acid immobilization. Due to that the boronic acid group on the surface of magnetic mesoporous silica nanocomposites can form tight yet reversible covalent bond with glycopeptides containing cis-1,2-diols groups, the magnetic mesoporous silica nanocomposites were successfully applied to selective enrichment of glycopeptides. APB functionalized magnetic mesoporous silica was also demonstrated to have high selectivity for the glycopeptides in the presence of a 10-fold excess bovine serum albumin （BSA） over horseradish peroxidase （HRP） in the tryptic digest. We also find that magnetic rnesoporous silica has better sensitivity in HRP digest compared with that of commercial aminophenylboronic acid-functionalized magnetic nanoparticles beads. The limit of detection for glycopeptides from glycoprotein HRP is about 0.01 ng/μL.

关键词： 3-aminophenylboronic acid monohydrate magnetic mesoporous silica selective enrichment glycopeptides mass spectrometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enzymatic recovery of glycopeptides from different industrial grades edible bird’s nest and its by-products:nutrient,probiotic and antioxidant activities,and physicochemical characteristics

引用

Food Science and Human Wellness 2022年第6期11卷 1555-1564页

作者： Hidayati Syamimi Mohd Noor Rafi dah Mohd Ariff Lee Sin Chang Xin Yi Chai Hui Yan Tan Nur’Aliah Daud Abdul Salam Babji Seng Joe Lim Faculty of Science and Technology Universiti Kebangsaan MalaysiaBangi 43600Malaysia International Institute for Halal Research and Training(INHART) International Islamic University Malaysia(IIUM)Jalan Gombak 53100Malaysia Faculty of Applied Sciences UCSI University Kuala LumpurCheras 56000Malaysia Faculty of Agriculture Science and Technology Universiti Putra Malaysia Bintulu CampusBintulu 97008Malaysia Innovative Centre for Confectionery Technology(MANIS) Faculty of Science and TechnologyUniversiti Kebangsaan MalaysiaBangi 43600Malaysia

This study was conducted to recover edible bird’s nest(EBN)hydrolysates from different grades of EBN,including the industrial by-products,using enzymatic *** nutrient,physicochemical properties and antioxidant activities of the recovered hydrolysates at different hydrolysis times were *** showed that the recovery yield of enzymatic hydrolysis was above 89%for all grades of EBN and the degree of hydrolysis increased over *** content(0.321-0.433 mg/L)was below the permissible tolerance level for all ***,the antioxidant activities(DPPH and ABTS scavenging activities and ferric reducing antioxidant powder(FRAP)activity)were significantly higher(P≤0.05)in hydrolysates recovered from EBN by-products(EBNhC and EBNhD)as compared to the high grade EBN hydrolysates(EBNhA and EBNhB).The in-vitro probiotic activity of EBN and its hydrolysates were examined using the probiotic bacterium Lactobacillus ***,EBN by-products hydrolysate(EBNhD)recorded the highest number of ***(1.1×1011 CFU/mL),indicating that low grade EBN has the potential as prebiotic material that promotes probiotic *** study demonstrated the concept of using EBN by-products hydrolysates for various applications,such as functional ingredients with enhanced bioactivities,to improve its economic value.

关键词： Edible bird’s nest by-products Enzymatic hydrolysis glycopeptides Lactobacillus plantarum Probiotic activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Natural products and drug discovery

引用

National Science Review 2022年第11期9卷 37-57页

作者： David J.Newman NIH Special Volunteer

This review covers the recent history of a series of very important natural products and their derivatives that are currently in use or under evaluation in the areas of anti-infectives, important cancer treatments that include antibody drug conjugates, followed by a discussion of type 2 diabetes(T2DM) drugs and angiotensin converting enzyme inhibitors. The current structures of the agents are shown, though in the case of some peptides used in T2DM drugs the standard single leTher abbreviation for an amino acid is used.

关键词： artemisinin glycopeptides anthracyclines dolastatin diabetes 2 ACE inhibitors

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Encouraging Solution to the Problem of Synthesizing Protein α-Thioester

引用

Chinese Journal of Chemistry 2024年第10期42卷 1114-1120页

作者： Xinliang Liu Zijun Gao Jie Zhao Farong Ye Ping Huang Ping Wang Center for Chemical Glycobiology Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsFrontiers Science Center for Transformative MoleculesSchool of Chemistry and Chemical EngineeringZhangjiang Institute for Advanced StudyShanghai JiaoTongUniversityShanghai 200240China Shenzhen Research Institute of Shanghai Jiao Tong University ShenzhenGuangdong 518057China

Expressed protein ligation(EPL)provides a powerful tool to access large-size proteins with precise *** methods for constructing the critical protein thioester for EPL have predominantly relied on the recombinant intein fusion expressed in Escherichia coli(***).Despite its powerful applications,the expression of thioester derived from eukaryotic protein in *** inherently suffers from its limited solubility,the inactivity of intein,premature hydrolysis and low *** overcome these obstacles,we present herein the facile one-flask synthesis of inaccessible proteinα-thioester via a SUMO-protein-intein(SPI)sandwich *** utility of SUMO enhances the protein fusion yield and solubility,prevents premature hydrolysis and simplifies the purification *** inaccessible protein thioester with internal Cys residues can be readily produced and is compatible with the EPL-desulfurization protocol used to prepare complex proteins,which is otherwise difficult to obtain using traditional *** utility has been highlighted through the synthesis of human granulocyte colony-stimulating factor(G-CSF).

关键词： Desulfurization Glycoproteins glycopeptides Protein expression Peptide thioester

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Facile Generation of Strained Peptidyl Thiolactones from Hydrazides and Its Application in Assembling MUC-1 VNTR OIigomers

引用

Chinese Journal of Chemistry 2021年第9期39卷 2509-2516页

作者： Yuankun Dao Biao Wang Weidong Dong Jun Zhang Chao Zhong Zhili Zhang Suwei Dong State Key Laboratory of Natural and Biomimetic Drugs and Department of Chemical BiologySchool of Pharmaceutical SciencesPeking UniversityBeijing 100191China Institute of Systems Biomedicine School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China

An efficient method for the activation of C-terminal 4-mecaptoproline-or penicillamine-containing peptide hydrazides in ligation re-actions is reported *** corresp on ding peptide hydrazides can be readily prepared using solid-phase peptide synthesis,and subsequently activated by acetylacet one(acac)without exoge nous thiol *** peptidyl thiolactones could be the possible reactive in termediates that drastically accelerate the reacti on rates at the sterically demandi ng proline and valine *** developed protocol allows for sequential peptide ligations in a one-pot manner,and expedites the assembly of mucin 1(MUC-1)variable number tandem repeat(VNTR)trimers in various glycosylated forms.

关键词： Strained molecules glycopeptides Synthetic methods Peptidyl thiolactones

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：