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Rapid Acclimation of Methanogenic Granular Sludge into Autotrophic Partial Denitrification Granules

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China Petroleum Processing & Petrochemical Technology 2019年第3期21卷 68-73页

作者： liu chunshuang Li Xuechen Zhang Xiaofei Bai Xue Guo Yadong Wang Yongxing Zhao Chaocheng College of Chemical Engineering China University of PetroleumQingdao 266580China CNPC Research Institute of Safety and Environmental Technology Beijing 102206

Rapid formation of autotrophic partial denitrification(APD)granules is of practical interest to start up an expanded granular sludge bed reactor for wastewater *** study demonstrates that methanogenic granules can be easily acclimated into autotrophic partial denitrification granules in one day,with the ability to remove 82%of 2.7 kg-S/(m^3·d)sulfide into S^0 and to convert 97%of 0.9 kg-N/(m^3·d)nitrate into nitrite,which can provide a promising feedstock for anaerobic ammonia oxidation *** *** essential for S^0 *** high loadings,the abundance of Arcobacter ***,while on the contrary the abundance of unclassified_p_Firmicutes increased,leading to the deterioration of autotrophic partial denitrification *** granules performance could be recovered by adopting the strategies of properly reducing the influent loadings.

关键词： methanogenic granules nitrite accumulation autotrophic partial denitri fication sul fide

Microbial Characterization of Denitrifying Sulfide Removal Sludge Using High-Throughput Amplicon Sequencing Method

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China Petroleum Processing & Petrochemical Technology 2015年第4期17卷 89-95页

作者： Ma Wenjuan liu chunshuang Zhao Dongfeng Guo Yadong Wang Aijie Jia Kuili College of Chemical Engineering China University of PetroleumQingdao State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

The denitrifying sulfide removal(DSR) process has recently been studied extensively from an engineering perspective. However, the importance of microbial communities of this process was generally underestimated. In this study, the microbial community structure of a lab-scale DSR reactor was characterized in order to provide a comprehensive insight into the key microbial groups in DSR system. Results from high-throughput sequencing analysis revealed that the fraction of autotrophic denitrifiers increased from 2.34 % to 10.93% and 44.51% in the DSR system when the influent Na Cl increased from 0 g/L, to 4 g/L and 30 g/L, respectively. On the contrary, the fraction of heterotrophic denitrifiers decreased from 61.74% to 39.57%, and 24.12%, respectively. Azoarcus and Thiobacillus were the main autotrophic denitrifiers, and Thauera was the main hetetrophic denitrifier during the whole process. This study could be useful for better understanding the interaction between autotrophs and heterotrophs in DSR system.

关键词： denitrifying sulfide removal autotrophic denitrifiers hetetrophic denitrifiers microbial communities

Investigation of Nitrite Production Pathway in Integrated Partial Denitrification/Anammox Process via Isotope Labelling Technique and the Relevant Microbial Communities

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China Petroleum Processing & Petrochemical Technology 2022年第1期24卷 129-134页

作者： Li Yanzhe Gai Jianing Zhang Xiaofei Zhao dongfeng Guo Yadong Yu Gengxing Zhao Chaocheng liu Fang Zhao Ruiyu liu chunshuang College of Chemical Engineering China University of PetroleumQingdao 266580 Research Institute of Safety and Environmental Technology Beijing 102206 Harbin Jianshi Technology Co. LtdHarbin 150040

In this study,the nitrogen removal performance of partial denitrificaiton/anammox(PDA)process was investigated by using an UASB *** total nitrogen(TN)removal efficiency(91.97%)was achieved at an influent nitrogen loading rate of 0.64 kg/(m3·d).Anammox bacteria did execute the function of converting nitrate to nitrite in PDA system according to ^(15)N isotope labeling experiments and the contribution was approximately 36.3%.Candidatus_Brocadia,Candidatus_Kuenenia and Thauera were functional strains for anammox and denitrification process,*** and Candidatus_Brocadia were more important for TN removal at high loading rates(0.64 kg/(m3·d)).This result can provide a theoretical and technical foundation for the application of the PDA process.

关键词： partial-denitrification anammox 15N isotope labeling experiments biological nitrogen removal

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大豆在储藏期间的品质变化

中国油脂 2009年第12期34卷 65-67页

作者：刘春双九三集团天津大豆科技有限公司天津300461

在深入了解大豆储藏期间品质变化的机理,准确把握大豆质变内在规律的基础上,结合当地气候条件,并依据储藏期内的具体情况,及时调整储藏工艺参数和技术指标,合理运用低温、低氧、干燥、通风、覆膜密闭等手段,调节外界环境影响因素,控制... 详细信息

在深入了解大豆储藏期间品质变化的机理,准确把握大豆质变内在规律的基础上,结合当地气候条件,并依据储藏期内的具体情况,及时调整储藏工艺参数和技术指标,合理运用低温、低氧、干燥、通风、覆膜密闭等手段,调节外界环境影响因素,控制大豆的温度、湿度,从而降低大豆的呼吸强度,抑制微生物和害虫的活动,有效减缓大豆品质的变化,保证大豆的安全储藏。

关键词：大豆储藏品质判定

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碳同位素标记法鉴别芳烃降解菌研究

中国石油大学学报（自然科学版） 2015年第6期39卷 57-62页

作者：胡恒宇赵东风刘春爽中国石油大学化学工程学院山东青岛266580

从油田采出水中富集培养石油烃降解菌,利用^(13) C标记的甲苯作为碳源和变性梯度凝胶电泳技术,从中鉴定和考察能够降解甲苯的细菌群落。结果表明:从采出水中富集培养的复合菌群经过500d的厌氧降解,对石油烃降解率达到36.4%,甲烷产量为20... 详细信息

从油田采出水中富集培养石油烃降解菌,利用^(13) C标记的甲苯作为碳源和变性梯度凝胶电泳技术,从中鉴定和考察能够降解甲苯的细菌群落。结果表明:从采出水中富集培养的复合菌群经过500d的厌氧降解,对石油烃降解率达到36.4%,甲烷产量为201.2μmol;以^(13) C标记的甲苯为碳源,经过60d的培养,复合菌群对其降解率达到67%,12 C标记的甲苯降解率为69%,两种碳源标记甲苯的降解率差异不显著;将离心中的重带^(13) C-DNA片段回收测序,鉴别出3种芳烃降解菌。

关键词：碳同位素菌株鉴定 CsCl密度梯度 DGGE分析芳烃

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最小相关结果集排序算法

哈尔滨工程大学学报 2013年第4期34卷 494-498,510页

作者：刘春双张志强谢晓芹黑河学院计算机科学与信息工程系黑龙江黑河164300 哈尔滨工程大学计算机科学与技术学院黑龙江哈尔滨150001

传统的信息检索系统都是按照结果与用户查询的相关性大小进行排序,返回结果之间相互孤立缺乏整体性,冗余性很大.为了使用户获得完整的相关信息,采用一种基于子主题提取的文档集合生成算法聚类相关检索结果,引入一个新概念"并集度",最后... 详细信息

传统的信息检索系统都是按照结果与用户查询的相关性大小进行排序,返回结果之间相互孤立缺乏整体性,冗余性很大.为了使用户获得完整的相关信息,采用一种基于子主题提取的文档集合生成算法聚类相关检索结果,引入一个新概念"并集度",最后利用相关度、并集度和新颖度对结果集重新排序.实验结果表明在进行算法改进后,整体相关度有所上升,同时排在前面的文档集合的并集度和新颖度也较大,包含的信息更加完整,冗余性更小,可以更好地满足用户的查询需求.

关键词：文档聚类检索结果集并集度新颖度

Pseudomonas ***-5产生的表面活性剂提取及其稳定性研究

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石油炼制与化工 2015年第2期46卷 18-21页

作者：李琳赵朝成刘其友刘春爽中国石油大学(华东)化学工程学院山东青岛266580

针对二苯并噻吩高效降解菌Pseudomonas ***-5降解过程中产生表面活性剂的现象,进行碳源优化,将提取分离出的表面活性剂进行化学组分分析和理化性质测定,考察温度、pH、无机离子对其表面活性稳定性的影响。结果表明,花生油为Pseudomonas ... 详细信息

针对二苯并噻吩高效降解菌Pseudomonas ***-5降解过程中产生表面活性剂的现象,进行碳源优化,将提取分离出的表面活性剂进行化学组分分析和理化性质测定,考察温度、pH、无机离子对其表面活性稳定性的影响。结果表明,花生油为Pseudomonas ***-5产生表面活性剂的最佳碳源,产生的表面活性剂为鼠李糖脂,产量为0.15g/L,临界胶束浓度（CMC）为180mg/L,亲水亲油平衡值（HLB）为12.3,对柴油24h的乳化能力达61%。该表面活性剂在温度30-80℃、pH 6-13的条件下表面活性稳定,能够耐受200g/L的NaCl或MgCl2以及20g/L的CaCl2,稳定性能良好。

关键词：假单胞菌生物表面活性剂鼠李糖脂稳定性

纳米ZnO-氧化石墨烯及ZnO-氧化石墨烯/水性聚氨酯复合涂层的抗菌性能

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复合材料学报 2018年第7期35卷 1930-1938页

作者：姜国飞李旭飞刘芳赵朝成王永强刘春爽中国石油大学(华东)化学工程学院青岛266580

循环冷却水系统滋生细菌会导致生物黏泥产生及设备腐蚀,为解决这一问题,由硅烷偶联剂γ-氨丙基三乙氧基硅烷(KH550)改性纳米ZnO,改性纳米ZnO与氧化石墨烯(GO)在二甲基乙酰胺中复合,获得纳米ZnOGO复合抗菌材料,并利用纳米ZnO-GO改性水性... 详细信息

循环冷却水系统滋生细菌会导致生物黏泥产生及设备腐蚀,为解决这一问题,由硅烷偶联剂γ-氨丙基三乙氧基硅烷(KH550)改性纳米ZnO,改性纳米ZnO与氧化石墨烯(GO)在二甲基乙酰胺中复合,获得纳米ZnOGO复合抗菌材料,并利用纳米ZnO-GO改性水性聚氨酯(PU),得到纳米ZnO-GO/PU复合涂层。对纳米ZnOGO复合抗菌材料进行表征分析及抗菌性能测试,对纳米ZnO-GO/PU复合涂层进行抗菌性能测试及物理性能分析。结果表明,纳米ZnO成功负载在GO表面,纳米ZnO-GO纯度较高,当GO质量分数为35wt%、纳米ZnOGO使用量为160mg·L-1时,其抗菌率可达97.16%;当纳米ZnO-GO质量分数为2.33wt%时,纳米ZnO-GO/PU复合涂层抗菌率可达90.29%,同时拥有4H的铅笔硬度及93.26%的缓蚀性能。

关键词：氧化石墨烯(GO) 纳米ZnO 复合材料复合涂层抗菌缓蚀

Tentative Study on a New Way of Simultaneous Desulfurization and Denitrification

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Chinese Journal of Chemical Engineering 2005年第3期13卷 422-425页

作者：王爱杰杜大仲任南琪程翔刘春爽 Department of Environmental Science & EngineeringSchool of Municipal & Environmental Engieering Harbin Institute of TechnologyHarbin 150090China

Thiobacillus denitrificans, a kind of autotrophic facultative bacteria, can oxidize sulfide into elemental sulfur or sulfate when nitrate was adopted as its electron accepter and carbon dioxide as its carbon resource under anoxic or anaerobic environment. In this way, nitrate is converted into nitrogen. In addition, ThiobaciWus denitrificans can accumulate sulfur extracellularly. In this study, in a process of simultaneous desulfurization and denitrification, a strain of Thiobacillus denitriificans is employed as sulfur-producer in the treatment of wastewater containing sulfide and nitrate. The key factors affecting this process are investigated through batch tests. The experimental results indicate that the sulfide concentration and the ratio of sulfide to nitrate (S2-/NO3-) in the influent are the key factors, and their suitable values are suggested to be 5/3 and no more than 300mg·L-1, respectively, in order to achieve high conversion of sulfur.

关键词： Thiobacillus denitrificans biological desulfurization sulfur denitrification S2 /NO3- ratio