检索结果-南通市图书馆

Induced Pluripotent Stem Cell-derived Mesenchymal Stem Cell Seeding on Biofunctionalized Calcium Phosphate Cements

在线全文

学校读者我要写书评

暂无评论

Bone Research 2013年第4期1卷 371-384页

作者： WahWah TheinHan Jun Liu Minghui Tang Wenchuan Chen linzhao cheng Hockin H.K.Xu Biomaterials & Tissue Engineering Division Department of EndodonticsProsthodontics and Operative DentistryUniversity of Maryland Dental School State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan University Stem Cell Program in Institute for Cell Engineering and Division of Hematology Johns Hopkins University Center for Stem Cell Biology & Regenerative Medicine University of Maryland School of Medicine University of Maryland Marlene and Stewart Greenebaum Cancer Center University of Maryland School of Medicine Department of Mechanical Engineering University of Maryland

Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.

关键词： induced pluripotent stem cells RGD fibronectin platelet concentrate biofunctionalized calciumphosphate cement bone tissue engineering

Engineered extracellular vesicles enable high-efficient delivery of intracellular therapeutic proteins

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2024年第10期15卷 724-743页

作者： Ding Ma An Xie Jiahui Lv Xiaolin Min Xinye Zhang Qian Zhou Daxing Gao Enyu Wang Lei Gao linzhao cheng Senquan Liu Department of Hematology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Blood and Cell Therapy Institute Anhui Provincial Key Laboratory of Blood Research and ApplicationsUniversity of Science and Technology of ChinaHefei 230036China School of Basic Medical Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China School of Biomedical Engineering Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China

Developing an intracellular delivery system is of key importance in the expansion of protein-based therapeutics acting on cytosolic or nuclear ***,extracellular vesicles(EVs)have been exploited as next-generation delivery modalities due to their natural role in intercellular communication and ***,fusion of protein of interest to a scaffold represents a widely used strategy for cargo enrichment in EVs,which could compromise the stability and functionality of ***,we report intracellular delivery via Ev-based approach(IDEA)that efficiently packages and delivers native proteins both in vitro and in vivo without the use of a *** a proof-of-concept,we applied the IDEA to deliver cyclic GMP-AMP synthase(cGAS),an innate immune *** results showed that cGAS-carrying EVs activated interferon signaling and elicited enhanced antitumor immunity in multiple syngeneic tumor *** cGAS EVs with immune checkpoint inhibition further synergistically boosted antitumor efficacy in ***,scRNA-seq demonstrated that cGAS EVs mediated significant remodeling of intratumoral microenvironment,revealing a pivotal role of infiltrating neutrophils in the antitumor immune ***,IDEA,as a universal and facile strategy,can be applied to expand and advance the developmentof protein-based therapeutics.

关键词： extracellular vesicles intracellular protein delivery cGAS cancer immunotherapy

Serum IgA,IgM,and IgG responses in COVID-19

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2020年第7期17卷 773-775页

作者： Huan Ma Weihong Zeng Hongliang He Dan Zhao Dehua Jiang Peigen Zhou linzhao cheng Yajuan Li Xiaoling Ma Tengchuan Jin Department of Obstetrics and Gynecology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001 AnhuiChina Hefei National Laboratory for Physical Sciences at Microscale Laboratory of Structural ImmunologyCAS Key Laboratory of Innate Immunity and Chronic DiseaseDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027 AnhuiChina Department of Infectious Diseases The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001 AnhuiChina Kangrun Biotech LTD Guangzhou 511400 GuangdongChina Department of Statistics University of Wisconsin-MadisonMadisonWI 53706USA Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei 230027 AnhuiChina Johns Hopkins University School of Medicine ColumbiaMDUSA Department of Clinical Laboratory The First Affiliated Hospital of Anhui Medical UniversityHefei 230032China Department of Laboratory Medicine The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001 AnhuiChina

Currently,detecting SARS-CoV-2 RNAs is a standard approach for COVID-19 ***,there is an urgent need for reliable and rapid serological diagnostic methods to screen SARS-CoV-2-infected people including those who do not have overt *** emerging studies described serological tests based on detection of SARS-CoV-2-specific IgM and IgG.1–4 Although detection of SARS-CoV-2-specific IgA in serum has been reported in few papers,5,6 analyses of IgA levels in a larger number of COVID-19 patients are still lacking.

关键词： Serum infected COV

Decline of SARS-CoV-2-specific IgG,IgM and IgA in convalescent COVID-19 patients within 100 days after hospital discharge

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第3期64卷 482-485页

作者： Huan Ma Dan Zhao Weihong Zeng Yunru Yang Xiaowen Hu Peigen Zhou Jianping Weng linzhao cheng Xueying Zheng Tengchuan Jin The First Affiliated Hospital of USTC Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Hefei National Laboratory for Physical Sciences at Microscale Laboratory of Structural ImmunologyCAS Key Laboratory of Innate Immunity and Chronic DiseaseDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230027China Department of Pulmonary and Critical Care Medicine the First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001China Department of Statistics University of Wisconsin-MadisonMadisonWI 53706USA Joint Laboratory of Public Health University of Science and Technology of China and Health Commission of Anhui ProvinceHefei 230026China Clinical Research Hospital(Hefei)of Chinese Academy of Science Hefei 230001China Division of Life Sciences and Medicine University of Science and Technology of ChinaHefei Anhui 230026China Johns Hopkins University School of Medicine BaltimoreMD 21205USA CAS Center for Excellence in Molecular Cell Science Chinese Academy of SciencesShanghai 200031China

Dear editor,The 2019 novel coronavirus(later renamed as SARS-CoV-2 in February 2020)infected over 12 million people globally by early July 2020,causing mild to severe COVID-19 in *** the levels of antibodies such as immunoglobin(Ig)G,M and A that are specific to SARS-CoV-2 and present in the blood provides not only an alternative method for diagnosing SARS-CoV-2 infection(including asymptomatic carriers),but also a simple way to monitor immune responses in convalescent patients or after vaccination.A high and persistent level of antibodies specific to SARS-CoV-2,especially those that can bind to and neutralize the virus,would be a strong indication that an immunized host could resist to SRAS-CoV-2 ***,there are no effective drugs to specifically prevent or cure SARS-CoV-2 infection;therefore,host immune responses and antibody-based therapeutics will continue to play important roles in combating and later preventing COVID-19.

关键词： patients drugs editor

In memory of Hal ***,a pluripotent scientist,pioneer,and mentor

在线全文

学校读者我要写书评

暂无评论

Blood Science 2022年第1期4卷 1-4页

作者： linzhao cheng Bin Guo Xinxin Huang Tao cheng Division of Life Sciences and Medicine in the University of Science and Technology of China ChinaJohns Hopkins University School of MedicineUSA Shanghai Jiao Tong University School of Medicine China Zhongshan-Xuhui Hospital and Institutes of Biomedical Sciences Fudan UniversityShanghaiChina Editor in Chief Blood ScienceInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeChina

With great sadness,we residing in China heard the news that Professor Hal *** passed away on December 8,2021,due to complications of thyroid *** was *** regret that we could not say goodbye in person to Hal,as he was fondly called by his students and colleagues around the *** wish to express publicly our heartfelt gratitude to Hal for his many seminal contributions to the scientific research and applications,and profound impact on the careers of younger scientists like *** are sure that our feeling and gratitude are also shared by hundreds of youngsters who are fortunate to have interactions with *** will continue to be a bright star in the sky,inspiring and guiding us for many years to come.

关键词： hundreds younger continue

Questions about NgAgo

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Protein & Cell 2016年第12期7卷 913-915页

作者： Shawn Burgess linzhao cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang National Human Genome Research Institute NIH Bethesda MD 20892 USA Division of Hematology in Department of Medicine Johns HopkinsUniversity School of Medicine Baltimore MD 21205 USA Stem Cell Program in the Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of LifeSciences Sun Yat-sen University Guangzhou 510275 China HIT Center of Life Sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking Univer- sity Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking Univer-sity Beijing 100871 China Center for Comparative Biomedicine Institute of SystemsBiomedicine Shanghai Jiao Tong University Shanghai 200240China Institute of Biophysics Chinese Academy of Sciences Beijing100101 China Institute of Molecular Medicine Peking University Beijing100871 China College of Engineering Peking University Beiiing 100871 China Laboratory of Disease Models in Non-Human Primates Instituteof Neuroscience Shanghai Chinese Academy of SciencesShanghai 200031 China College of Life Sciences Peking University Beijing 100871 China

Dear Editor： Gao et al. published data in Nature Biotechnology （Nat Biotechnol. 2016 May 2） showing that DNA-guided genome editing using the Natronobacterium gregoryi Argonaute （NgAgo） protein targeted 47 mammalian genomic loci with a 100% success rate and an efficiency of 21.3%-41.3% at various targets. This report led us to test NgAgo＇s utility in various cells and organisms such as mouse and zebrafish for gene editing.

关键词：

在线全文

学校读者我要写书评

暂无评论

人类细胞外囊泡的产量优化与应用

中国生物工程杂志 2024年第9期44卷 1-13页

作者：王晨蕾程临钊刘森泉中国科学技术大学生命科学与医学部合肥230027

目的:构建与优化细胞外囊泡(extracellular vesicles,EVs)的高产平台,验证此平台是否适用于功能蛋白的装载与递送。方法:通过实时荧光定量PCR(qRT-PCR)、流式细胞术或Western blot(WB)检测细胞目的基因表达,采用差速超速离心法浓缩EVs,... 详细信息

目的:构建与优化细胞外囊泡(extracellular vesicles,EVs)的高产平台,验证此平台是否适用于功能蛋白的装载与递送。方法:通过实时荧光定量PCR(qRT-PCR)、流式细胞术或Western blot(WB)检测细胞目的基因表达,采用差速超速离心法浓缩EVs,分别利用WB与纳米颗粒跟踪分析(nanoparticle tracking analysis,NTA)检测EVs标记蛋白表达、粒径分布与产量。利用脂多糖(lipopolysaccharides,LPS)诱导的A549细胞损伤模型验证EVs递送功能蛋白的能力。通过CCK8检测细胞活力,通过Annexin V检测细胞凋亡,利用qRT-PCR检测Wnt/β-catenin下游基因AXIN2、TCF4、NKD1的表达。结果:流式细胞术结果表明CD9、CD81均能在HEK293T和HEK293F上过表达,NTA检测显示CD9更有助于EVs产量的提高。实现在贴壁细胞体系中EVs产量提升4倍,在悬浮细胞体系中EVs产量提升6倍。之后验证高产平台是否适用于功能蛋白Wnt3a的装载与递送。WB结果证实此高产平台能装载Wnt3a蛋白,NTA检测表明此高产平台在装载Wnt3a的同时将EVs产量提升3倍左右。CCK8、Annexin V结果显示CD9 Wnt3a EVs能明显提高A549细胞在LPS诱导损伤后的细胞活力,抑制细胞凋亡。机制上,qRT-PCR表明CD9 Wnt3a EVs激活了Wnt/β-catenin下游基因AXIN2、TCF4、NKD1的表达。结论:构建了一种基于CD9过表达的EVs高产平台,该平台对EVs产量的提升在贴壁细胞与悬浮细胞中均得到了验证,该平台对功能蛋白Wnt3a的递送能力在LPS诱导的A549细胞损伤模型中得到了验证。该平台为实现EVs量产提供了新策略,有望成为一种新的功能蛋白递送平台,在生物学研究和无细胞治疗领域开拓更多应用。

关键词：细胞外囊泡 CD9 CD81 Wnt3a

Erratum to： Questions about NgAgo

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2017年第1期8卷 77-77页

作者： Shawn Burgess linzhao cheng Feng Gu Junjiu Huang Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang National Human Genome Research Institute NIH Bethesda MD USA Division of Hematology in Department of Medicine Johns Hopkins University School of Medicine Baltimore MD 21205 USA Stem Cell Program in the Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of Life Sciences Sun Yat-sen University Guangzhou 510275 China HIT Center of Life Sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking University Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking University Beijing 100871 China Center for Comparative Biomedicine Institute of Systems Biomedicine Shanghai Jiao Tong University Shanghai 200240 China Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China Institute of Molecular Medicine Peking University Beijing 100871 China College of Engineering Peking University Beijing 106871 China Laboratory of Disease Models in Non-Human Primates Institute of Neuroscience Shanghai Chinese Academy of Sciences Shanghai 200031 China College of Life Sciences Peking University Beijing 100871 China

In the original publication of this article the Figure 1E legend has been incorrectly published.

关键词： Erratum Questions NgAgo

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

管线渗漏影响下盾构引起管-土变形试验研究

铁道工程学报 2023年第5期40卷 110-115页

作者：施鑫荣传新王厚良孙学军崔林钊程桦蔡海兵安徽理工大学淮南232001 安徽建工集团股份有限公司蚌埠233000

研究目的:在非饱和地层盾构下穿既有管线过程中,管道本身若存在长期“暗渗”,将使得管–隧间土体稳定性大大降低,当盾构开挖面接近管线下方时,容易引起地层及管线过度沉降,进而引发工程事故。为研究非饱和黏土地层管线渗漏影响下隧道盾... 详细信息

研究目的:在非饱和地层盾构下穿既有管线过程中,管道本身若存在长期“暗渗”,将使得管–隧间土体稳定性大大降低,当盾构开挖面接近管线下方时,容易引起地层及管线过度沉降,进而引发工程事故。为研究非饱和黏土地层管线渗漏影响下隧道盾构施工扰动引起的地层及管线变形规律,开展盾构施工与管线渗漏耦合作用三维模型试验,分析管线渗漏水扩散规律、施工全过程中地层沉降发展规律以及管线应力应变特征。研究结论:(1)最终渗漏水扩散范围以竖向为主,横向扩散长度增加19%,竖向扩散长度增加46%;(2)渗漏水降低了管线底部土体抗剪强度,在隧道开挖扰动下,弱化后的地层稳定性受到更大的影响,随着深度的增加影响越大,模型试验中测得地层中心埋深330 mm处(原型9.9 m)在单、双线隧道贯通后沉降量分别增加64.6%和45.4%;(3)受隧道二次开挖重复扰动的影响,双线隧道贯通后的管线应变值远高于单线隧道开挖,地层沉降及管线变形呈现“U”形,管线最大拉应力位置偏向于先开挖的左线隧道;(4)本研究成果可为相似条件下盾构下穿给排水管线的稳定控制提供借鉴和参考。

关键词：盾构渗漏模型试验地层沉降管线应变