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Serum proteins differentially expressed in gestational diabetes mellitus assessed using isobaric tag for relative and absolute quantitation proteomics

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World Journal of Clinical Cases 2024年第8期12卷 1395-1405页

作者： Wei-li Cao Cui-Ping Yu ling-li zhang Department of Women’s Health Care Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070Hubei ProvinceChina Obstetrical Department The First People’s Hospital of Jiangxia District Wuhan City(Union Jiangnan Hospital Huazhong University of Science and Technology)Wuhan 430200Hubei ProvinceC Department of Gynecology Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070Hubei ProvinceChina

BACKGROUND As a well-known fact to the public,gestational diabetes mellitus(GDM)could bring serious risks for both pregnant women and *** this important investigation into the linkage between GDM patients and their altered expression in the serum,proteomics techniques were deployed to detect the differentially expressed proteins(DEPs)of in the serum of GDM patients to further explore its pathogenesis,and find out possible biomarkers to forecast GDM *** Subjects were divided into GDM and normal control groups according to the IADPSG diagnostic *** samples were randomly selected from four cases in each group at 24-28 wk of gestation,and the blood samples were identified by applying iTRAQ technology combined with liquid chromatography-tandem mass *** proteins and signaling pathways associated with GDM were identified by bioinformatics analysis,and the expression of key proteins in serum from 12 wk to 16 wk of gestation was further verified using enzyme-linked immunosorbent assay (EliSA).RESULTS Forty-seven proteins were significantly differentially expressed by analyzing the serum samples between the GDMgravidas as well as the healthy ones. Among them, 31 proteins were found to be upregulated notably and the rest16 proteins were downregulated remarkably. Bioinformatic data report revealed abnormal expression of proteinsassociated with lipid metabolism, coagulation cascade activation, complement system and inflammatory responsein the GDM group. EliSA results showed that the contents of RBP4, as well as ANGPTL8, increased in the serumof GDM gravidas compared with the healthy ones, and this change was found to initiate from 12 wk to 16 wk *** GDM symptoms may involve abnormalities in lipid metabolism, coagulation cascade activation, complementsystem and inflammatory response. RBP4 and ANGPTL8 are expected to be early predictors of GDM.

关键词： Gestational diabetes mellitus liquid chromatography-tandem mass spectrometry Isobaric tag for relative and absolute quantitation Proteomics Biomarker

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Skeletal phenotypes and molecular mechanisms in aging mice

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Zoological Research 2024年第4期45卷 724-746页

作者： Qiao Guan Yuan zhang Zhi-Kun Wang Xiao-Hua liu Jun Zou ling-li zhang School of Exercise and Health Shanghai University of SportShanghai 200438China College of Athletic Performance Shanghai University of SportShanghai 200438China

Aging is an inevitable physiological process,often accompanied by age-related bone loss and subsequent bone-related diseases that pose serious health *** on skeletal diseases caused by aging in humans is challenging due to lengthy study durations,difficulties in sampling,regional variability,and substantial ***,mice are preferred for such studies due to their similar motor system structure and function to humans,ease of handling and care,low cost,and short generation *** this review,we present a comprehensive overview of the characteristics,limitations,applicability,bone phenotypes,and treatment methods in naturally aging mice and prematurely aging mouse models(including SAMP6,POLG mutant,LMNA,SIRT6,ZMPSTE24,TFAM,ERCC1,WERNER,and KL/KL-deficient mice).We also summarize the molecular mechanisms of these aging mouse models,including cellular DNA damage response,senescence-related secretory phenotype,telomere shortening,oxidative stress,bone marrow mesenchymal stem cell(BMSC)abnormalities,and mitochondrial ***,this review aims to enhance our understanding of the pathogenesis of aging-related bone diseases.

关键词： Aging Premature aging Mice Bone Gene knockout

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Correlation analysis of human papillomavirus E6/E7 mRNA detection with diagnosis,prognosis and recurrence risk in patients with cervical epithelioma

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World Journal of Clinical Cases 2024年第20期12卷 4146-4153页

作者： ling-li zhang Ming-Yan Du Xin Du Jie Duan Dong-Mei Yao Jing Jing Chun Feng lin Song Department of Gynaecology Maternal and Child Health Hospital of Hubei ProvinceWuhan 430075Hubei ProvinceChina Department of Gynaecology China Resources WISCO General HospitalWuhan 430080Hubei ProvinceChina Department of Surgery Maternal and Child Health Hospital of Hubei ProvinceWuhan 430075Hubei ProvinceChina

BACKGROUND Cervical intraepithelial neoplasia(CIN)is an important precursor of cervical *** detection and treatment can reduce the incidence of cervical *** To investigate the detection rate of human papillomavirus(HPV)E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia(CIN)and its relationship with CIN progression and *** One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively *** patients were graded CIN based on colposcopy and cervical *** positive expression rates of HPV E6/E7 mRNA and HPV[polymerase chain reaction(PCR)-reverse dot crossing]were compared among all *** with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 *** relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate *** The diagnostic sensitivity,specificity,and sensitivity of PCR-reverse point hybrid ization technology for secondary CIN were 70.41%,70.66%,and 0.714,*** and specificity for secondary CIN were 752%and 7853%,respectively,the area under the curve value was *** Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I(P<0.05).In CIN grade I patients with positive for HPV E6/E7 mRNA,in its orientation to grade CIN patients,in its orientation to grade CIN patients,at 69.2%,compared with patients negative for HPV E6/E7 mRNA(30.8%),significant difference(P<0.05).CONCLUSION HPV E6/E7 mRNA and HPV(PCR-reverse dot hybrid)positive expression have a close relationship with CINgrade disease progression and is an independent risk factor for high-grade CIN lesions.

关键词： Cervical epithelial neoplasia Human papilloma change with virus E6/E7 mRNA Cervical cancer Correlation analysis

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Dynamic recrystallization mechanism, texture evolution development and mechanical characteristics of a Mg–8.7Gd–4.18Y–0.42Zr magnesium alloy by ECAP

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Progress in Natural Science:Materials International 2024年第2期34卷 376-388页

作者： ling zhang Yinglong li School of Materials Science and Engineering Northeastern University School of Mechanical and Engineering Ningxia Institute of Science and Technology

Equal channel angle pressing is recognized for its ability to refine alloy grains and alter grain orientation, thereby achieve better mechanical performance of the magnesium alloy. This study investigates the microstructures,dynamic recrystallization mechanism, texture development, and mechanical performance of GW94K(Mg–8.7Gd–4.18Y–0.42Zr wt. %) Mg alloy following ECAP-4 passes at 400°C and 3 mm/min. Results show that when high-temperature deformation is undertaken, twin formation is suppressed while dislocation slip is facilitated, increasing dislocation density during deformation. Following ECAP deformation, the sample displayed higher fracture elongation, TYS, and UTS than the as-solutioned GW94K alloy. In particular, the GW94K alloy performed well mechanically after ECAP-4 passes, with an ultimate TYS of 231 MPa, an UTS of 290 MPa, and an elongation of 14.8 %. DDRX and shear bands induce CDRX, both of which are important in plastic deformation. as well as in modifying microstructure and grain orientation during ECAP deformation.

关键词： Dynamic recrystallization mechanism Equal channel angular pressing Magnesium alloy Mechanical performance Mg-Gd-Y-Zr alloy Texture development

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Tel1 and Rif2 oppositely regulate telomere protection at uncapped telomeres in Saccharomyces cerevisiae

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Journal of Genetics and Genomics 2018年第9期45卷 467-476页

作者： ling-li zhang Zhenfang Wu Jin-Qiu Zhou CAS Center for Excellence in Molecular Cell Science Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sdences Shanghai 200031 China School of life Science and Technology Shanghai Tech University Shanghai 201210 China

It has been well documented that Tel1 positively regulates telomere-end resection by promoting Mre11-Rad50-Xrs2（MRX） activity, while Rif2 negatively regulates telomere-end resection by inhibiting MRX activity. At uncapped telomeres, whether Tel1 or Rif2 plays any role remains largely unknown. In this work, we examined the roles of Tel1 and Rif2 at uncapped telomeres in yku70△ and/or cdc13-1 mutant cells cultured at non-permissive temperature. We found that deletion of TEL1 exacerbates the temperature sensitivity of both yku70△ and cdc13-1 cells. Further epistasis analysis indicated that MRX and Tel1 function in the same pathway in telomere protection. Consistently, TEL1 deletion increases accumulation of Exo1-dependent telomeric single-stranded DNA（ssDNA） at uncapped telomeres, which stimulates checkpoint-dependent cell cycle arrest. Moreover, TEL1 deletion in yku70△ cells facilitates Rad51-dependent Y0 recombination. In contrast, RIF2 deletion in yku70△ cells decreases the accumulation of telomeric ssDNA after 8 h of incubation at the non-permissive temperature of 37℃ and suppresses the temperature sensitivity of yku70△ cells, likely due to the increase of Mre11 association at ***, our findings indicate that Tel1 and Rif2 regulate telomere protection at uncapped telomeres via their roles in balancing MRX activity in telomere resection.

关键词： Tel1 RiF2 MRX Uncapped telomeres Telomere protection

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Blimp-1 protein and Hans classification on prognosis of diffuse large B-cell lymphoma and their interrelation

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Chinese Journal of Cancer 2010年第9期29卷 781-786页

作者： Yan Song Zhi Cao ling li Hong-Tu zhang Xun zhang Department of Pathology Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing100021 P. R. China

Background and Objective:Diffuse large B-cell lymphoma (DLBCL), the most common type of non-Hodgkin's lymphoma (NHL), is heterogeneous on molecular and clinical levels, therefore, its prognosis is difficult to *** study aimed to evaluate the value of Blimp-1 protein and Hans classification in predicting the prognosis of DLBCL and their ***:The clinical records of 136 patients with DLBCL were *** patients were followed up for 5-80 months (median, 39 months).Immunohistochemical staining for CD10, MUM1, Bcl-6, and Blimp-1 were performed on paraffin-embedded tumor tissues from the 136 *** correlations of Blimp-1 protein and Hans classification in prognosis of DLBCL and their interrelation were ***:Blimp-1 was detected in 38 (30.0%) patients, and was associated with a significantly shorter overall survival (OS) (P=0.030).Using the Hans classification based upon the expression of CD10, Bcl-6, and MUM1, 54 patients had germinal center B-cell (GCB) phenotype and 82 had non-GCB *** 5-year OS rate was 75% in the GCB group and 52% in the non-GCB group (P=0.020).The positive rate of Blimp-1 was 22.2% in the GCB group and 31.7% in the non-GCB group (P=0.329).The Cox regression multivariate analysis showed that international prognosis index (IPI) and Hans classification had independent prognostic significance, whereas Blimp-1 was not an independent prognostic ***:The patients with GCB subtype of DLBCL had better prognosis than the non-GCB *** level of Blimp-1 expression in the patients with DLBCL implies a shorter survival, but it is not associated with Hans classification.

关键词： B细胞淋巴瘤分类价值弥漫性预后飞艇蛋白发电机断路器免疫组织化学染色

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Serum Spondin-2 expression,tumor invasion,and antitumor immune response in patients with cervical cancer

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World Journal of Clinical Cases 2022年第36期10卷 13304-13312页

作者： ling-li zhang Song lin Yan zhang Dong-Mei Yao Xin Du Department of Gynecology Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070Hubei ProvinceChina Department of Surgery Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070Hubei ProvinceChina Department of Women’s Health Maternal and Child Health Hospital of Hubei ProvinceWuhan 430070Hubei ProvinceChina

BACKGROUND Cervical cancer is a gynecological malignancy common in middle-aged and older patients,with a high mortality ***-2 is an extracellular matrix protein that involved in innate and acquired immune ***,we investigated the relationship between serum Spondin-2 expression,tumor invasion and infiltration,and immune response in patients with cervical cancer and provided a theoretical basis for clinical *** To investigate the relationship between serum Spondin-2 expression and cervical cancer-related *** Overall,147 patients with cervical cancer who were admitted to our institution between January 2019 and August 2019 were assigned to the cervical cancer group,and 92 patients with benign uterine lesions and 86 healthy individuals were assigned to the benign and control groups,*** each group,serum Spondin-2 expression was measured,and the receiver operating characteristic(ROC)curve was *** with cervical cancer were classified into high or low Spondin-2 groups depending on the Spondin-2 threshold value used for diagnosing cervical ***’s clinical data were collected to compare the clinicopathologic characteristics,immune cytokine levels,and prognosis of patients with varying Spondin-2 expression *** The expression level of serum Spondin-2 was significantly higher in the cervical cancer group than in the benign and control groups(P<0.05).According to the ROC curve,the cutoff value of Spondin-2 used in the diagnosis of cervical carcinoma was 25.68±7.11μg/*** proportion of patients with Federation of Gynecology and Obstetrics stage III,nerve invasion,vascular invasion,and lymph node metastasis was higher in the high Spondin-2 group than in the low Spondin-2 group(P<0.05).Interleukin-5(IL-5)and IL-4 Levels were higher in the high Spondin-2 group than in the low Spondin-2 *** contrast,IL-2 and tumor necrosis factor-αlevels were lower in the high Spondin-2 group than in th

关键词： Cervical cancer Spondin-2 Invasion and infiltration Immune response Prognosis Malignant tumor

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Image Field Deformation of LAMOST due to Differential Atmospheric Refraction

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Chinese Journal of Astronomy and Astrophysics 2006年第4期6卷 495-502页

作者： Jin-ling li Bo zhang Yong Yu Zhao-Xiang Qi Ming Zhao Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030

A vectorial expression of the image field deformation of LAMOST due to the differential atmospheric refraction is presented. The calculated results are compared with those from previous analyses based on the traditional spherical trigonometric formulas. It is demonstrated that different tangential displacements of star images during the observation tracking given by various authors are simply due to different reference points adopted. It is pointed out that the observational celestial pole is the center of the apparent diurnal motion, that, by referring to the observational celestial pole, the effect of the differential refraction on the image field of LAMOST during the 1.5-hour tracking period is approximately equivalent to a constant rotation of - 13.65″ for all declination belts. It is therefore unnecessary to design a particular tracking velocity for each observation, and this will be obviously advantageous to the observation implementation. If the maximum tracking error of the fibers is 0.2″, then the fibers are required to be able to re-position during observational tracking for sky regions south of declination ＋20° and north of declination ＋60°.

关键词： telescopes -- atmospheric effects -- methods: analytical-- methods: statistical-- astrometry

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Microwave-Promoted Rapid Synthesis of 1-Aryl-1,2,3-Triazoles

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Chinese Chemical Letters 2001年第9期12卷 763-764页

作者： Lan TAO ling li zhang Shui Jun SHEN Xiao Ping HAN Department of Chemistry Hangzhou Teachers CollegeHangzhou 310036

Aryl azides and a-keto phosphorus ylides were reacted within 4 similar to 10 minutes with silica gel support, under microwave irridiation to afford corresponding 1-aryl-1, 2, 3-triazoles in moderate to good yields.

关键词： Microwave triazole

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light(Science & Applications) 2024年第2期13卷 333-346页

作者： Yifan Sun Qian li ling-Jun Kong Xiangdong zhang Key Laboratory of advanced optoelectronic quantum architecture and measurements of Ministry of Education Beijing Key Laboratory of Nanophotonics&Ultrafine Optoelectronic SystemsSchool of PhysicsBeijing Institute of Technology100081BeijingChina

Compared with electrical neural networks,optical neural networks(ONNs)have the potentials to break the limit of the bandwidth and reduce the consumption of energy,and therefore draw much attention in recent *** far,several types of ONNs have been ***,the current ONNs cannot realize the acceleration as powerful as that indicated by the models like quantum neural *** to construct and realize an ONN with the quantum speedup is a huge ***,we propose theoretically and demonstrate experimentally a new type of optical convolutional neural network by introducing the optical *** is called the correlated optical convolutional neural network(COCNN).We show that the COCNN can exhibit“quantum speedup”in the training *** character is verified from the two *** is the direct illustration of the faster convergence by comparing the loss function curves of the COCNN with that of the traditional convolutional neural network(CNN).Such a result is compatible with the training performance of the recently proposed quantum convolutional neural network(QCNN).The other is the demonstration of the COCNN’s capability to perform the QCNN phase recognition circuit,validating the connection between the COCNN and the ***,we take the COCNN analog to the 3-qubit QCNN phase recognition circuit as an example and perform an experiment to show the soundness and the feasibility of *** results perfectly match the theoretical *** proposal opens up a new avenue for realizing the ONNs with the quantum speedup,which will benefit the information processing in the era of big data.

关键词： process quantum convolution

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