检索结果-南通市图书馆

Small moleculeα-methylene-γ-butyrolactone,an evolutionarily conserved moiety in sesquiterpene lactones,ameliorates arthritic phenotype via interference DNA binding activity of NF-κB

引用

Acta Pharmaceutica Sinica B 2024年第8期14卷 3561-3575页

作者： Kegang linghu Wenqing Cui Taiqin Li Yueting Tuo Dasong Wang Huiqi Pan Tian Zhang Ligen lin Hua Yu Xiaoxia Hu Haiyang Li Xiangchun shen Department of Surgery the Affiliated Hospital of Guizhou Medical UniversityGuiyang 550001China The Key Laboratory of Optimal Utilization of Natural Medicine Resources School of Pharmaceutical SciencesGuizhou Medical UniversityGuian New DistrictGuizhou 561113China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR 999078China Guizhou Institute of Precision Medicine Affiliated Hospital of Guizhou Medical UniversityGuiyang 550001China

Rheumatoid arthritis(RA)is an inflammatory disease accompanied by abnormal synovial microenvironment(SM).Sesquiterpene lactones(SLs)are the main anti-inflammatory ingredients of many traditional herbs utilized in RA treatment.α-Methylene-γ-butyrolactone(α-M-γ-B)is a core moiety that widely exists in natural *** study was designed to investigate the anti-arthritic potential ofα-M-γ-B as an independent small molecule in vitro and in vivo.α-M-γ-B exhibited stronger electrophilicity and anti-inflammatory effects than the other six analogs.α-M-γ-B inhibited the production of pro-inflammatory mediators via repolarizing M1 macrophages into M2 *** transcriptome sequencing suggested thatα-M-γ-B regulated the immune system ***,α-M-γ-B attenuated collagen type II-induced arthritic(CIA)phenotype,restored the balance of Tregs-macrophages and remodeled SM via repolarizing the synovial-associated macrophages in CIA ***,althoughα-M-γ-B did not prevent the trans-nucleus of NF-κB it interfered with the DNA binding activity of NF-κB via direct interaction with the sulfhydryl in cysteine residue of NF-κB p65,which blocked the activation of NF-κ*** of NF-κB reduced the M1 polarization of macrophage and suppressed the synovial hyperplasia and angiogenesis.α-M-γ-B failed to ameliorate CIA in the presence of N-acetylcysteine or when the mice were subjected to the macrophage-specific deficiency of *** conclusion,α-M-γ-B significantly attenuated the CIA phenotype by directly targeting NF-κB p65 and inhibiting its DNA binding *** results suggest thatα-M-γ-B has the potential to serve as an alternative candidate for treating *** greater electrophilicity ofα-M-γ-B,the basis for triggering strong anti-inflammatory activity,accounts for the reason whyα-M-γ-B is evolutionarily conserved in the SLs by medical plants.

关键词： a-Methylene-gbutyrolactone Rheumatoid arthritis NF-κB p65 Synovial microenvironment Sesquiterpene lactones

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Targeted DNA N^(6)-methyladenine editing by dCas9 fused to METTL4 in the lepidopteran model insect Bombyx mori

引用

Insect Science 2024年第2期31卷 646-650页

作者： Feng Chen Hao Guo Weiqun Lan Mingyi Zhou Wenjng Geng Guanwang shen Ping lin Qingyou Xia Ping Zhao Zhiqing Li Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City Biological Science Research CenterSouthwest UniversityChongqingChina Chongqing Key Laboratory of Sericultural Science Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqingChina

Dear Editor,N^(6)-Methyladenine(6mA)DNA modification is an important epigenetic mechanism with roles in regulating gene expression,nucleosome positioning,DNA damage repair,and cell cycle progression(Heyn&Esteller,2015;Luo et al.,2015;Boulias&Greer,2022).Despite progress in understanding the biological functions of 6mA,the contribution of individual 6mA installations on site-specific target genes is largely unknown,and therefore deciphering the molecular mechanism of 6mA in target gene expression has been difficult.

关键词： adenine fused Bombyx

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibition of mGluR1 alters the excitability of the commissural pyramidal neuron in the rat anterior cingulate cortex after chronic constriction injury to the sciatic nerve

Inhibition of mGluR1 alters the excitability of the commissu...

引用

中国神经科学学会第十二届全国学术会议

作者： Shi-Hao Gao lin-lin shen Hui-Zhong Wen Yan-Dong Zhao Huai-Zhen Ruan Department of Neurobiology College of Basic Medical Science Chongqing Key Laboratory of Neurobiology Third Military Medical University Department of Neurology Daping Hospital Third Military Medical University

Objective Our previous study has shown that inhibition of the metabotropic glutamate receptor subtype 1（mGluR1） in the output side of rat anterior cingulate cortex（ACC） has therapeutic implications for the sustained nociceptive hypersensitivity. However, it still remains unclear whether the ACC has different subtypes of output neurons. Furthermore, the specific changes in different subtypes of ACC layer 5 pyramidal neurons（L5 PNs） during chronic pain, as well as the distinct effect of mGluR1 inhibition on different neuronal subtypes, have not been well studied. Methods Retrograde labeling combined with immunofluorescence and whole-cell clamp recording were performed to characterize the alterations of commissural ACC L5 PNs in a rat model of chronic constriction injury（CCI） to the sciatic nerve. The specific agonist and antagonist were applied to evaluate the contribution of mGluR1 to these alterations and the underlying mechanisms. Behavioral tests combined with RNA interference were performed to test whether mGluR1 antagonist exerts analgesic effect via its action on the commissural neurons. Results Commissural L5 PNs（projecting to contralateral cortex） existed in the ACC and exhibited few hyperpolarization-activated/cyclic nucleotide-gated channels. The voltagegated potassium channel subunit 2（Kv2）-mediated current in these neurons were substantially reduced after CCI（current densities at +30 m V for the sham and CCI neurons were [mean ± SD] 10.22 ± 3.42 p A/p F vs. 5.58 ± 2.71 p A/p F, respectively;n = 11, p ＜ 0.01）, which increased the spike width and fast afterhyperpolarization potential, resulting in hyperexcitability. Activating mGluR1 in sham slices could mimic the downregulation of Kv2 currents, but inhibition of mGluR1 alleviated the downregulation of Kv2 currents（current density increased by 8.11 ± 3.22 p A/p F;n = 7, p ＜ 0.01） in CCI slices, thereby restoring the normal spike width and fast afterhyperpolarization potential. Protein kinase C mediated the

关键词： neuropathic pain anterior cingulate cortex commissural pyramidal neuron metabotropic glutamate receptor subtype 1 voltage-gated potassium channel subunit 2

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Polarization-dependent photoinduced metal–insulator transitions in manganites

引用

Science Bulletin 2024年第2期69卷 183-189页

作者： lina Deng Weiye Zhang Hanxuan lin Lifen Xiang Ying Xu Yadi Wang Qiang Li Yinyan Zhu Xiaodong Zhou Wenbin Wang Lifeng Yin Hangwen Guo Chuanshan Tian Jian shen State Key Laboratory of Surface Physics Institute for Nanoelectronic Devices and Quantum Computingand Department of PhysicsFudan UniversityShanghai 200433China Shanghai Research Center for Quantum Sciences Shanghai 201315China Zhangjiang Fudan International Innovation Center Fudan UniversityShanghai 201210China Collaborative Innovation Center of Advanced Microstructures Nanjing 210093China Shanghai Branch CAS Center for Excellence and Synergetic Innovation Center in Quantum Information and Quantum PhysicsShanghai 201315China

In correlated oxides,collaborative manipulation on light intensity,wavelength,pulse duration and polarization has yielded many exotic discoveries,such as phase transitions and novel quantum *** view of potential optoelectronic applications,tailoring long-lived static properties by light-induced effects is highly *** far,the polarization state of light has rarely been reported as a control parameter for this ***,we report polarization-dependent metal-to-insulator transition(MIT)in phaseseparated manganite thin films,introducing a new degree of freedom to control static ***,we observed giant photoinduced resistance jumps with striking features:(1)a single resistance jump occurs upon a linearly polarized light incident with a chosen polarization angle,and a second resistance jump occurs when the polarization angle changes;(2)the amplitude of the second resistance jump depends sensitively on the actual change of the polarization *** transmittance measurements reveal that the origin of the above phenomena is closely related to the coexistence of anisotropic *** results represent a first step to utilize light polarization as an active knob to manipulate static phase transitions,pointing towards new pathways for nonvolatile optoelectronic devices and sensors.

关键词： Metal-insulator phase transitions Polarization of light Electronic phase separation Selective domain controllability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Salt-assisted vapor-liquid-solid growth of high-quality ultrathin nickel oxide flakes for artificial synapses in image recognition applications

引用

Nano Research 2024年第5期17卷 4622-4630页

作者： Hui Zhang Zongjie shen Alei Li lin Wang Qinan Wang Yunfei Li Yunlei Zhong Juntong Zhu Yong Zhang Mengjiao Han Dan Tian Chun Zhao Lixing Kang Qingwen Li School of Physical Science and Technology Shanghai Tech UniversityShanghai 201210China Division of Advanced Materials Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China College of Energy Soochow Institute for Energy and Materials Innovationsand Key Laboratory of Advanced Carbon Materials and Wearable Energy Technologies of Jiangsu ProvinceSoochow UniversitySuzhou 215123China Songshan Lake Materials Laboratory Dongguan 523808China School of Advanced Technology Xi’an Jiaotong-Liverpool UniversitySuzhou 215123China College of Materials Science and Engineering Co-Innovation Center of Efficient Processing and Utilization of Forest ResourcesNanjing Forestry UniversityNanjing 210037China

Transition metal oxides have attracted intense interest owing to their abundant physical and chemical *** controlled preparation of large-area,high-quality two-dimensional crystals is essential for revealing their inherent properties and realizing high-performance ***,fabricating two-dimensional(2D)transition metal oxides using a general approach still presents substantial ***,we successfully achieve highly crystalline nickel oxide(NiO)flakes with a thickness as thin as 3.3 nm through the salt-assisted vapor-liquid-solid(VLS)growth method,which demonstrated exceptional stability under ambient *** explore the great potential of the NiO crystal in this work,an artificial synapse based on the NiO-flake resistive switching(RS)layer is ***-term and long-term synaptic behaviors are obtained with external *** artificial synaptic performance provides the foundation of the neuromorphic application,including handwriting number recognition based on artificial neuron network(ANN)and the virtually unsupervised learning capability based on generative adversarial network(GAN).This pioneering work not only paves new paths for the synthesis of 2D oxides in the future but also demonstrates the substantial potential of oxides in the field of neuromorphic computing.

关键词： nickel oxide(NiO)flakes vapor-liquid-solid(VLS)growth memristor synapse generative adversarial network(GAN)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nutritional intervention based on curcumin-loaded polysaccharides nanoparticle for synergetic improvement of ulcerative colitis

引用

Food Science and Human Wellness 2024年

作者： Jun Yang Jieqiong lin Yi Luo Xianxiang Chen Mingyue shen Yuanxing Wang Jianhua Xie State Key Laboratory of Food Science and Resources， Nanchang University

The prevalence of ulcerative colitis (UC) is increasing annually， while current non-targeted drugs for UC have limited effectiveness， easily relapsed， and serious side effects. Herein， curcumin (Cur)-loaded nanoparticle with conlon-targeted property based on Mesona chinensis polysaccharides was developed for the synergistic and targeted improvement of UC. Results show that ZmNPs have good encapsulation of Cur， targeted delivery of Cur to the colon， and prolonged its retention time. In vivo safety assessments have shown that ZmNPs have good safety and biocompatibility. As expected， Cur-ZmNPs effectively alleviated the symptoms of DSS-induced UC by decreasing colonic inflammation by inhibiting the TLR4/MAPK pathway， regulating the levels of oxidative stress and immune homeostasis of UC mice. Oral administration of Cur-ZmNPs can reduce apoptosis of intestinal epithelial cells， alleviate colonic mucosal damage and repair intestinal barrier function. Cur-ZmNPs also had a positive effect on improving gut microbiota disorders and promoting the production of SCFAs. This study provides a novel strategy for synergistic alleviation of UC by MCP-based NPs loaded with food bioactives.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Biomimetic Phosphohydrolase Nanozyme Based on Defect-Engineered Metal-Organic Framework

引用

CCS Chemistry 2024年第7期6卷 1821-1835页

作者： Xiaoxue Kou Yuhong lin Yong shen linjing Tong Rui Gao Suya Liu Siming Huang Fang Zhu Guosheng Chen Gangfeng Ouyang MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510006 Shanghai NanoPort Thermo Fisher ScientificPudong DistrictShanghai 200120 Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target and Clinical Pharmacology The NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and The Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436

Phosphodiester bonds are the backbone linkages of all the nucleic acids which store and transfer biological *** hydrolysis of phosphodiester bonds is of great importance to the replication,recombination,and damage repair of DNA as well as the maturation and processing of ***,the spontaneous scission of individual phosphodiester linkages is kinetically challenging under physiological conditions,with an estimated half-life of 30 million ***,we discover a defect-engineered Zr-metal-organic framework(Zr-MOF)nanozyme containing cluster-missing reo defects,named UiO-66 REO,that possesses intrinsic phosphodiesterase-like activity that allows for the cleavage of the phosphodiester bond in physiological condition(pH=7.4 and 37℃),outperforming the activityrecording *** atomic-level structure of the UiO-66 REO nanozyme has been directly identified by the synchrotron radiation absorption spectrum and integrated differential phase contrast-scanning transmission electron *** find that both the defective boundary regions and unusual Zr6-clusters formed in the reo phase of the UiO-66 REO nanozyme contribute to the improvement in efficiency of phosphodiesterase-like hydrolysis,which realizes the cleavage of DNA in mild *** work offers a new insight into biomimetic enzymology using crystal and defect engineering and opens up new possibilities for the development of low-cost,structurally stable and sustainable phosphodiesterase nanozymes for different biological applications.

关键词： nanozyme metal-organic framework biomimetic catalysis hydrolase-like activity defective engineering

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The spatial transcriptomic landscape of human gingiva in health and periodontitis

引用

Science China(Life Sciences) 2024年第4期67卷 720-732页

作者： Zongshan shen Ran Zhang Yunjia Huang Jiayao Chen Mengjun Yu Chunhua Li Yong Zhang lingling Chen Xin Huang Jichen Yang Zhengmei lin Songlin Wang Bin Cheng Hospital of Stomatology Guangdong Provincial Key Laboratory of StomatologyGuanghua School of StomatologySun Yat-sen UniversityGuangzhou 510055China Beijing Laboratory of Oral Health Capital Medical UniversityBeijing 100050China Department of Oral Pathology Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijing 100050China Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions Chinese Academy of Medical SciencesBeijing 100050China BGI-Qingdao BGI-ShenzhenQingdao 266555China

The gingiva is a key oral barrier that protects oral tissues from various stimuli.A loss of gingival tissue homeostasis causes periodontitis,one of the most prevalent inflammatory diseases in *** human gingiva exists as a complex cell network comprising specialized *** understand the tissue-specific pathophysiology of the gingiva,we applied a recently developed spatial enhanced resolution omics-sequencing(Stereo-seq)technique to obtain a spatial transcriptome(ST)atlas of the gingiva in healthy individuals and periodontitis *** utilizing Stereo-seq,we identified the major cell types present in the gingiva,which included epithelial cells,fibroblasts,endothelial cells,and immune cells,as well as subgroups of epithelial cells and immune *** further observed that inflammation-related signalling pathways,such as the JAK-STAT and NF-κB signalling pathways,were significantly upregulated in the endothelial cells of the gingiva of periodontitis patients compared with those of healthy ***,we characterized the spatial distribution of periodontitis risk genes in the gingiva and found that the expression of IFI16 was significantly increased in endothelial cells of inflamed *** conclusion,our Stereo-seq findings may facilitate the development of innovative therapeutic strategies for periodontitis by mapping periodontitis-relevant genes and pathways and effector cells.

关键词： spatial transcriptomics gingiva periodontal disease periodontitis risk genes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Medcheck:A novel software for automated de-formulation of traditional Chinese medicine(TCM)prescriptions by liquid chromatography-mass spectrometry

引用

Journal of Pharmaceutical Analysis 2024年第6期14卷 930-932页

作者： Xiao-lan Li Jian-qing Zhang Yun Li Xuan-jing shen Huan-ya Yang lin Yang Meng Xu Qi-rui Bi Chang-liang Yao De-an Guo Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Research Center of TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China b University of Chinese Academy of Sciences Beijing100049China

Prescriptions are the main clinical application of traditional Chinese medicines(TCMs).Common forms include Chinese patent medicines,Kampo formulas,and hospital decoctions.A new pre-scription called“famous classical formulas”is recently developed and expected to boom in the *** constituent me-dicinal plants in prescriptions is critical for new drug development and quality control[1],which could avoid safety issues from adulteration or substandard ingredients,as seen in the notorious Longdan Xiegan Pill event.

关键词： medicines formulation prescription

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

MDGA2 Constrains Glutamatergic Inputs Selectively onto CA1 Pyramidal Neurons to Optimize Neural Circuits for Plasticity,Memory,and Social Behavior

引用

Neuroscience Bulletin 2024年第7期40卷 887-904页

作者： Xuehui Wang Donghui lin Jie Jiang Yuhua Liu Xinyan Dong Jianchen Fan Lifen Gong Weida shen linghui Zeng Tonghui Xu Kewen Jiang Steven A.Connor Yicheng Xie School of Life Sciences Nanchang UniversityNanchang330031China Department of Neurology Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Biology York University4700 Keele StreetTorontoON M3J 1P3Canada Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province School of MedicineHangzhou City UniversityHangzhou 310015China

Synapse organizers are essential for the development,transmission,and plasticity of *** as rare synapse suppressors,the MAM domain containing glycosylphosphatidylinositol anchor(MDGA)proteins contributes to synapse organization by inhibiting the formation of the synaptogenic neuroligin-neurexin complex.A previous analysis of MDGA2 mice lacking a single copy of Mdga2 revealed upregulated glutamatergic synapses and behaviors consistent with ***,MDGA2 is expressed in diverse cell types and is localized to both excitatory and inhibitory *** the network versus cell-specific effects of MDGA2 loss-of-function requires a cell-type and brain region-selective *** address this,we generated mice harboring a conditional knockout of Mdga2 restricted to CA1 pyramidal *** we report that MDGA2 suppresses the density and function of excitatory synapses selectively on pyramidal neurons in the mature *** deletion of Mdga2 in CA1 pyramidal neurons of adult mice upregulated miniature and spontaneous excitatory postsynaptic potentials,vesicular glutamate transporter 1 intensity,and neuronal *** effects were limited to glutamatergic synapses as no changes were detected in miniature and spontaneous inhibitory postsynaptic potential properties or vesicular GABA transporter ***,evoked basal synaptic transmission and AMPAR receptor currents were enhanced at glutamatergic *** a behavioral level,memory appeared to be compromised in Mdga2 cKO mice as both novel object recognition and contextual fear conditioning performance were impaired,consistent with deficits in long-term potentiation in the CA3-CA1 *** affiliation,a behavioral analog of social deficits in autism,was similarly *** results demonstrate that MDGA2 confines the properties of excitatory synapses to CA1 neurons in mature hippocampal circuits,thereby optimizing this network for plasticity,c

关键词： MDGA2 CA1 pyramidal neurons Glutamatergic inputs Synaptic plasticity Memory Social behaviors Autism Synapse organizers

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：