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Design and Synthesis of a Novel Peptidomimetic Inhibitor of Caspase-3

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Chemical Research in Chinese Universities 2006年第2期22卷 225-228页

作者： ZHANG Jin-liang LIU lin-hua GUO yang-hong yang Jin-gang LI Wei ZHONG Da-fang WANG Ji-dong College of Life Science Jilin University Changchun 130021 P. R. China

Caspases, a family of cysteine proteases, comprise of highly homologous enzymes that play an important role in apoptotic cell death. Caspase-3 shows key functions in apoptosis, mediating apoptotic cascade from the intrinsic and extrinsic activation pathways. Therefore, caspase-3 is an attractive target for therapeutic intervention. For instance, inhibitors of caspase-3 have been described as promising cardioprotectants, neuroprotectants and antiarthritic agents. A novel peptidomimetic inhibitor of caspase-3, has been designed, which still has the properties of a reversible inhibitor, while the P1 site at the C-terminal remains, and only L-amino acid has been replaced by D-amino acid. Also presented here is the synthesis of the inhibitor and its inhibitory activity against caspase-3, which was tested by the fluorescent activity assay.

关键词： Caspase-3 Inhibitor Retro-inverso Peptidomimetie Synthesis

Guided chemotherapy based on patient-derived mini-xenograft models improves survival of gallbladder carcinoma patients

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Cancer Communications 2018年第1期38卷 511-519页

作者： Ming Zhan Rui-meng yang Hui Wang Min He Wei Chen Sun-wang Xu lin-hua yang Qiang Liu Man-mei Long Jian Wang Department of Biliary-Pancreatic Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong University160 Pujian RoadShanghai 200127P.R.China Department of Pathology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127P.R.China Department of Pathology Shanghai Ninth People’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200011P.R.China

Background:Gallbladder carcinoma is highly aggressive and resistant to chemotherapy,with no consistent strategy to guide first line ***,patient-derived xenograft(PDX)model has been increasingly used as an effective model for in preclinical study of ***:Mini-PDX model was established using freshly resected primary lesions from 12 patients with gallblad-der to examine the sensitivity with five of the most commonly used chemotherapeutic agents,namely gemcitabine,oxaliplatin,5-fluorouracil,nanoparticle albumin-bound(nab)-paclitaxel,and *** results were used to guide the selection of chemotherapeutic agents for adjunctive treatment after the ***-Meier method was used to compare overall survival(OS)and disease free survival(DFS)with 45 patients who received conventional chemo-therapy with gemcitabine and ***:Cell viability assays based on mini-PDX model revealed significant heterogeneities in drug ***-Meier analysis showed that patients in the PDX-guided chemotherapy group had significantly longer median OS(18.6 months;95%CI 15.9-21.3 months)than patients in the conventional chemotherapy group(13.9 months;95%CI 11.7-16.2 months)(P=0.030;HR 3.18;95%CI 1.47-6.91).Patients in the PDX-guided chemotherapy group also had significantly longer median DFS(17.6 months;95%CI 14.5-20.6 months)than patients in the conventional chemotherapy group(12.0 months;95%CI 9.7-14.4 months)(P=0.014;HR 3.37;95%CI 1.67-6.79).Conclusion:The use of mini-PDX model to guide selection of chemotherapeutic regimens could improve the out-come in patients with gallbladder carcinoma.

关键词： Gallbladder cancer Mini-PDX Chemosensitivity Overall survival Personalized therapy

Deciphering the role of mtH2O2 in hepatic ischemia-reperfusion injury mechanisms with low-background super-resolution fluorogenic probe

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Science China Chemistry 2025年第1期68卷 297-307页

作者： Bin Fang Limin Wang Haoqin Li Jiaxin Zhang yang Ding Pan Pan Li Bo Peng hua Bai lin Li Frontiers Science Center for Flexible Electronics Xi'an Institute of Flexible Electronics (IFE) and Xi'an Institute of Biomedical Materials &Engineering Northwestern Polytechnical University Institute of Flexible Electronics (IFE Future Technologies) Xiamen University

Hepatic ischemia-reperfusion injury（HIRI） is characterized by mitochondrial dysfunction and oxidative stress. Monitoring mitochondrial hydrogen peroxide（mtH2O2） levels in real-time through super-resolution imaging is crucial for elucidating its distribution in live cells and its mechanism of action during HIRI. However, low-background fluorogenic probes have been overlooked in the context of super-resolution imaging. In this study, we developed a low-background fluorogenic probe（MitoWG） with the potential for super-resolution morphology-correlated mitochondrial identification to track the fluctuates of mtH2O2in HIRI. Activation of the desirable fluorescence properties of the probe by mtH2O2was confirmed using structural illumination microscopy（SIM）, enabling high-quality mitochondrial imaging with exceptional specificity and *** in mtH2O2levels were successfully observed in both cellular and rat models of HIRI. Furthermore, we associated the decline in mitochondrial redox homeostasis with accelerated mtH2O2production during HIRI, which triggered mitophagy deficiency and led to cell death. In conclusion, Mito-WG possesses excellent photophysical and low-background properties for SIM imaging, making it a promising tool for mtH2O2tracking in HIRI research and clinical diagnosis.

关键词： hepatic ischemia-reperfusion injury low-background mtH2O2-activatable super-resolution imaging fluorogenic probe

Compound heterozygous p.L483P and p.S310G mutations in GBA1 cause type 1 adult Gaucher disease:A case report

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World Journal of Clinical Cases 2022年第36期10卷 13426-13434页

作者： Xiao-ling Wen Yao-Zi Wang Xia-lin Zhang Jia-Qiang Tu Zhi-Juan Zhang Xia-Xia Liu Hai-Yan Lu Guo-Ping Hao Xiao-huan Wang lin-hua yang Rui-Juan Zhang Department of Hematology The First People’s Hospital of YibinYibin 644000Sichuan ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Third Hospital of Shanxi Medical UniversityThe Shanxi Bethune HospitalThe Shanxi Academy of Medical SciencesThe Tongji Shanxi HospitalThe Shanxi Medical UniversityTaiyuan 030032Shanxi ProvinceChina Department of Hematology The Children’s Hospital of ShanxiTaiyuan 030006Shanxi ProvinceChina

BACKGROUND Gaucher disease(GD)is caused by a GBA1 gene mutation that leads to decreased acidβ-glucosidase activity[glucocerebrosidase(GCase)].This study aimed to identify and characterise compound heterozygous mutations in GBA1 in a patient with type 1 *** SUMMARY Here,we report a rare adult-onset type 1 GD in a 46-year-old female patient with clinical manifestations of giant spleen,thrombocytopenia,and bone pain,diagnosed by enzymatic and genetic *** and whole exome sequencing revealed heterozygous missense mutations in exon 10 c.1448T>C(p.L483P)and exon 7 c.928A>G(p.S310G)of *** latter was first reported in patients with *** modelling showed that p.S310G and p.L483P were distant from the GCase active *** p.S310G mutation in domain 1 may decrease stability between theα2 andα3 helices of *** p.L483P mutation in domain 2 reduced the van der Waals force of the side chain and disrupted the C-terminalβ-*** patient was treated with imiglucerase replacement therapy,and her condition was *** The p.L483P/p.S310G novel compound heterozygous mutation underlies type 1 GD and likely affects GCase protein *** is the first description of p.S310G being associated with mild type 1 GD in the context of a coinherited p.L483P mutation.

关键词： Gaucher disease Parkinson’s disease Lipid metabolism Molecular mechanism Case report

Study the Photoelectric Overall Technology by Using System Optimization Idea

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Systems Science and Systems Engineering 2000年第3期10卷 380-385页

作者： JIANG Hui\|lin,\ yang hua\|min,\ XI Hao Changchun Institute of Optics and Fine Mechanics, Changchun 130022, China

In this paper, we try to study the photoelectric overall technology by using system optimization idea. The evaluation indices of photoelectric system′s overall performance are clarified, and then the problems of syst... 详细信息

关键词： photoelectric overall technology system optimization

Effect of Satellite Vibration on Performance of Free-space Optical Communication

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通讯和计算机（中英文版） 2007年第1期4卷 41-45页

作者： YIN Ke-xin JIANG Hui-lin yang hua-min WU Qiong QI Yu-lan Department of Optical Electronic Engineering Changchun University of Science and Technology Changchun 130022 China

老年人日间肛肠手术中等比重罗哌卡因单次腰麻合理剂量的应用及其安全性评价

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吉林大学学报（医学版） 2012年第6期38卷 1196-1200页

作者：林华阳林洁饶福东杨锡馨陈刚林玮卢希福建中医药大学附属人民医院麻醉科福建福州350004

目的:探讨老年人日间肛肠手术中等比重罗哌卡因头高足低体位单次腰麻的合理剂量,并对其安全性进行评价,为老年人日间肛肠手术的临床麻醉提供依据。方法:老年肛肠病手术患者120例随机分为罗哌卡因0.8、1.0、1.3和1.5mL组,每组30例,各组... 详细信息

目的:探讨老年人日间肛肠手术中等比重罗哌卡因头高足低体位单次腰麻的合理剂量,并对其安全性进行评价,为老年人日间肛肠手术的临床麻醉提供依据。方法:老年肛肠病手术患者120例随机分为罗哌卡因0.8、1.0、1.3和1.5mL组,每组30例,各组患者均给予1%罗哌卡因与脑脊液分次混合液,经蛛网膜下腔分别注入0.8、1.0、1.3和1.5mL,观察患者麻醉效果、生命体征、麻醉平面固定时间、术后麻醉完全恢复时间、不良反应等。结果:随着罗哌卡因剂量增加,各组患者生命体征变化和感觉平面固定时间比较差异均无统计学意义(P>0.05);最高阻滞平面≤T5-8发生率变化比较,罗哌卡因1.3和1.5mL组均大于罗哌卡因0.8和1.0mL组(P<0.05);罗哌卡因1.5mL组感觉阻滞维持时间最长,与其他3组比较差异有统计学意义(P<0.05);术中肌松程度比较,罗哌卡因0.8mL组欠佳,而罗哌卡因1.0、1.3、1.5mL组均能满足手术要求,与罗哌卡因0.8mL比较差异有统计学意义(P<0.05);罗哌卡因0.8mL组术中牵拉腹痛发生率高,与其他3组比较差异有统计学意义(P<0.05);罗哌卡因1.3和1.5mL组运动神经阻滞完全消退时间最长,与罗哌卡因0.8mL组比较差异有统计学意义(P<0.05),但罗哌卡因1.5mL组患者需术后导尿的发生率最高,与其他3组比较差异有统计学意义(P<0.05)。结论:老年人日间肛肠手术应用1%罗哌卡因分次脑脊液稀释实施头高足底体位下等比重单次腰麻剂量以1.0～1.3mL为宜,患者生命体征平稳,不良反应少。

关键词：罗哌卡因蛛网膜下腔阻滞,单次合理剂量肛肠手术

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泰宁梅林戏文创产品设计与实践

包装工程 2017年第22期38卷 203-206页

作者：文艳群林阳华三明学院三明365004

目的发掘泰宁梅林戏的独特性,探讨其文创产品设计策略与原则,指导设计实践。方法分析泰宁梅林戏的文化内涵和艺术特色,提出泰宁梅林戏文创产品设计策略,归纳泰宁梅林戏文创产品形用统一和形意统一设计原则,并以"戏中情"礼品设计和"梅人... 详细信息

目的发掘泰宁梅林戏的独特性,探讨其文创产品设计策略与原则,指导设计实践。方法分析泰宁梅林戏的文化内涵和艺术特色,提出泰宁梅林戏文创产品设计策略,归纳泰宁梅林戏文创产品形用统一和形意统一设计原则,并以"戏中情"礼品设计和"梅人胚"化妆品设计作为设计实践。结论泰宁梅林戏文创产品设计应注重对文化本原的传承与创新。

关键词：泰宁梅林戏文创产品设计实践

Improvement of the Second Order Approximation of the Smoothed Particle Hydrodynamics

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Journal of Shanghai Jiaotong university(Science) 2008年第4期13卷 404-407页

作者：陈思周岱董石麟李华峰阳光 School of Naval Architecture Ocean and Civil EngineeringShanghai Jiaotong University

The smoothed particle hydrodynamics (SPH), as a fully Lagrangian particle method, has been suc- cessfully applied to astrophysical problems and extended to elastic dynamics and computational fluid dynamics. High order derivatives have to be approximated when elastic dynamics problems are modeled. However, the approximation errors in SPH could lead to computational failure in the case that the order of derivative is high. A novel method was proposed in order to improve the accuracy of SPH method, which shows the relationship between the selected functions and their SPH approximations. The entire involved system was represented by a finite number of particles that carry individual mass and occupy individual space, and the integral interpo- lation was approximated by a summation interpolation. In addition, error comparison was made between SPH method with and without the present improvement.

关键词： smoothed particle hydrodynamics (SPH) second order derivatives approximation error analysis