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Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9- Guided Hyperactive hAID Mutant

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Molecular Plant 2018年第4期11卷 623-626页

作者： Bin Ren Fang Yan yongjie Kuang Na Li Dawei Zhang Xueping Zhou Honghui lin Huanbin Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China Ministry of Education Key Laboratory of Bio-Resource and Eco-Environment College of Life Sciences Sichuan University Chengdu 610065 China State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 China

Dear Editor Single-nucleotide polymorphism （SNP） is an abundant form of genetic variation among individuals in plant species, which has been believed to have effects on the gene function responsible for phenotypic diversity. Utilization of functional SNPs associated with diverse important agronomic traits is of great value in crop improvements. Recently, a simple base editing system, which in- troduces a single-nucleotide change in genes of interest for generating gain-of-function mutants, has been developed in plants （Li et al., 2017; Lu and Zhu, 2017; Ren et ai., 2017）, in which Cas9（D10A） nickase is engineered with the rat cytosine deaminase （APOBEC1） and the uracil glycosylase inhibitor （UGI）. Under the guidance of sgRNA.

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A smart dual-drug nanosystem based on coassembly of plant and food-derived natural products for synergistic HCC immunotherapy

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Acta Pharmaceutica Sinica B 2021年第1期11卷 246-257页

作者： Bingchen Zhang Jiali Jiang Pengyu Wu Junjie Zou Jingqing Le Juanfang lin Chao Li Bangyue Luo yongjie Zhang Rui Huang Jingwei Shao Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy College of ChemistryFuzhou UniversityFuzhou 350116China Institute of Oceanography Minjiang UniversityFuzhou 350108China

Nanotechnology has emerged as an ideal approach for achieving the efficient chemo agent ***,the potential toxicity and unclear internal metabolism of most nano-carriers was still a major obstacle for the clinical ***,a novel"core-shell"co-assembly carrier-free nanosystem was constructed based on natural sources of ursolic acid(UA)and polyphenol(EGCG)with the EpCAM-aptamer modification for hepatocellular carcinoma(HCC)synergistic *** the nature products derived from food-plant,UA and EGCG had good anticancer activities and low *** the simple and"green"method,the nanodrugs had the advantages of good stability,p H-responsive and strong penetration of tumor tissues,which was expected to increase tumor cellular uptake,long circulation and effectively avoid the potential defects of traditional *** nanocomplex exhibited the low cytotoxicity in the normal cells in vitro,good biosafety of organic tissues and efficient tumor accumulation in ***,UA combined with EGCG showed the immunotherapy by activating the innate immunity and acquired immunity resulting in significant synergistic therapeutic *** research could provide new ideas for the research and development of self-assembly delivery system in the future,and offer effective intervention strategies for clinical HCC treatment.

关键词： Natural product Ursolic acid EGCG Aptamer Co-assembly Nanodrug HCC Synergistic treatment Immunotherapy

Remediation of mercury-contaminated soils and sediments using biochar:a critical review

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Biochar 2021年第1期3卷 23-35页

作者： Qian Yang yongjie Wang Huan Zhong State Key Laboratory of Pollution Control and Resources Reuse School of the EnvironmentNanjing University163 Xian Lin Da DaoNanjing 210023People’s Republic of China Key Laboratory of Geographic Information Science Ministry of EducationSchool of Geographic SciencesEast China Normal UniversityShanghai 200241People’s Republic of China College of Biological and Environmental Engineering Binzhou UniversityBinzhou 256600People’s Republic of China Environmental and Life Sciences Program(EnLS) Trent UniversityPeterboroughONCanada

The transformation of mercury(Hg)into the more toxic and bioaccumulative form methylmercury(MeHg)in soils and sediments can lead to the biomagnification of MeHg through the food chain,which poses ecological and health *** the last decade,biochar application,an in situ remediation technique,has been shown to be effective in mitigating the risks from Hg in soils and ***,uncertainties associated with biochar use and its underlying mechanisms ***,we summarize recent studies on the effects and advantages of biochar amendment related to Hg biogeochemistry and its bioavailability in soils and sediments and systematically analyze the progress made in understanding the underlying mechanisms responsible for reductions in Hg *** existing literature indicates(1)that biochar application decreases the mobility of inorganic Hg in soils and sediments and(2)that biochar can reduce the bioavailability of MeHg and its accumulation in crops but has a complex effect on net MeHg *** this review,two main mechanisms,a direct mechanism(e.g.,Hg-biochar binding)and an indirect mechanism(e.g.,biochar-impacted sulfur cycling and thus Hg-soil binding),that explain the reduction in Hg bioavailability by biochar amendment based on the interactions among biochar,soil and Hg under redox conditions are ***,the existing problems with the use of biochar to treat Hg-contaminated soils and sediments,such as the appropriate dose and the long-term effectiveness of biochar,are *** research involving laboratory tests and field applications is necessary to obtain a mechanistic understanding of the role of biochar in reducing Hg bioavailability in diverse soil types under varying redox conditions and to develop completely green and sustainable biochar-based functional materials for mitigating Hg-related health risks.

关键词： Biochar Methylmercury Soils Sediments Bioavailability

Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice

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Molecular Plant 2018年第4期11卷 631-634页

作者： Fang Yan yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui lin Bing Yang Xueping Zhou Huanbin Zhou State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricutural Sciences Beijing 100193 China Ministry of Education Key Laboratory of Bio-Resource and Eco-Environment College of Life Sciences Sichuan University Chengdu 610065 China College of Agronomy Henan University of Science and Technology Luoyang 471023 China Department of Genetics Development and Cell Biology Iowa State University Ames IA 50011 USA State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 China

Dear Editor The newly developed CRISPR/Cas9-mediated base editing technology with cytosine deaminase is capable of precisely and efficiently introducing point mutations at the target genomic locus, which does not require double-stranded DNA breaks or any donor templates and thus exhibit a great potential for gene correction and genetic diversification in yeasts, plants, and mammalian and human cells （Komor et al., 2016; Nishida et al., 2016; Lu and Zhu, 2017; Ren et al., 2017）.

关键词： CRISPR/Cas9 TadA base editing rice (Oryza sativa L.)

Analysis of functional hub genes indicates DLGAP5 is linked to lung adenocarcinoma prognosis

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BIOCELL 2023年第11期47卷 2453-2469页

作者： HAOSHENG ZHENG RUIJUN lin WEIJIE CAI YUZHEN ZHENG XINGPING YANG ZUI LIU FEI QIN yongjie CAI XIANYU QIN HONGYING LIAO Department of Thoracic Surgery Thoracic Cancer CenterThe Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhou510655China Department of Thoracic Surgery The First Affiliated Hospital of Shantou University Medical CollegeShantou515041China Department of Microbiology Zhongshan School of MedicineKey Laboratory for Tropical Diseases Control of the Ministry of EducationSun Yat-sen UniversityGuangzhouChina

Introduction:The difficulty in treating lung adenocarcinoma(LUAD)is caused by a shortage of knowledge about the biological mechanisms and a lack of treatment ***:The aim of this study was to identify a valuable molecular target for the treatment of ***:Using multiple databases,we screened for hub genes in LUAD using Cytoscape and explored the expression and prognosis of DLG associated protein 5(DLGAP5)in *** investigated the genetic variation,functional enrichment,and epigenetic activity of ***,we evaluated the relationship between the tumor microenvironment(TME)and ***:Our study identified 10 hub genes in LUAD:CDC45,KIAA0101,DLGAP5,CDT1,NCAPG,CCNB1,CDCA5,CDC20,KIF11,and *** discovered that DLGAP5 was overexpressed and associated with poor prognosis in ***5 exhibited an overall genetic variation frequency of 2%,and its DNA promoter was hypomethylated in LUAD(p<0.05).The expression of DLGAP5 in LUAD showed a positive correlation with the majority of N6-methyladenosine(m6A)-methylation ***,DLGAP5 was primarily associated with the cell cycle in ***,there was a significant favorable association between DLGAP5 and CD274,CTLA4,HAVCR2,and LAG3 in ***:DLGAP5 may be a therapeutic target for LUAD,as it affects cancer cells proliferation and development through the regulation of cell-cycle checkpoints and modulation of immune cell infiltration and immune checkpoints in the TME.

关键词： DLGAP5 Lung adenocarcinoma Prognosis Tumor microenvironment Hub genes

RNA‑Sequencing Reveals Gene Expression and Pathway Signatures in Umbilical Cord Blood Affected by Birth Delivery Mode

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Phenomics 2023年第3期3卷 228-242页

作者： yongjie Liu Kun Sun Yuexin Gan Han Liu Juehua Yu Wei Xu lin Zhang Dan Chen Ministry of Education and Shanghai Key Laboratory of Children’s Environmental Health Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Shenzhen Bay Laboratory Shenzhen 518107China Department of Obstetrics and Gynaecology The International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Centre for Experimental Studies and Research The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Department of Immunology School of Basic Medical SciencesFudan UniversityShanghai 200032China Department of Gynecology Third Xiangya Hospital of Central South UniversityChangsha 410013China Shanghai Fosun Pharmaceutical Industrial Development Co.LtdShanghai 200233China

Cesarean section(CS)confers increased risk of type I diabetes,asthma,inflammatory bowel disease,celiac disease,over-weight and obesity,etc.,in the ***,the underlying mechanism remains *** investigate the influence of CS on gene expression in cord blood,we have performed RNA-sequencing followed by single-gene analysis,gene set enrichment analysis,gene co-expression network analysis,and interacting genes/proteins analysis in eight full-term infants born by elective CS and eight matched vaginally delivered(VD)*** genes identified above were further vali-dated in another 20 CS and 20 VD *** found for the first time that mRNA expression of genes involved in immune response(IL12A,INFG,IL1B,TNF,MIF,IL4,CA1,IFI27,HLA-DOB and EPHB1)and metabolism(DLK1,CYP2A6 and GATM)were significantly influenced by ***,serum TNF-αand IFN-γwere remarkably up-regulated in the CS infants(p=5.0×10^(–4)and 3.0×10^(–3),respectively)compared to the VD *** is biologically plausible that CS may exert adverse impacts on offspring health through influencing expression of genes in the above *** findings will help understand the potential underlying mechanisms of the adverse health impacts of CS and identify biomarkers for future health of offspring born with different delivery modes.

关键词： Cesarean section Immune response Metabolism Ribosome Ephrin receptor signaling pathway

Tissue expression,association analysis between three novel SNPs of the RXRαgene and growth traits in Chinese indigenous cattle

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Chinese Science Bulletin 2013年第18期58卷 2053-2060页

作者： MA Yun GAO HanTing lin Feng CHEN NingBo XU yongjie JIANG JinHang LI Fen LU FangRu ZHAO Man SHI Kuilin CHENG Ni LI JunYa College of Life Sciences Xinyang Normal University College of Animal Husbandry and Veterinary Medicine Henan Agricultural University Animal Husbandry Bureau of Pingdingshan Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding Institute of Animal Husbandry and VeterinaryHubei Academy of Agricultural Science Institute of Animal Science Chinese Academy of Agricultural Sciences

Retinoid X receptor(RXR) α is a member of the nuclear hormone receptor superfamily that mediates the biological effects on several hormones,vitamins,and regulates lipid,glucose and energy *** this study,the tissue expression profiles of the bovine RXRαgene and association analysis of single nucleotide polymorphisms(SNPs) with growth traits were carried out in 413 Chinese native *** expression profile was analysed in ten Jiaxian cattle tissues by real-time PCR,and the results showed that RXRαgene was abundantly expressed in adipose tissue and spleen,moderately expressed in heart,liver,lung,kidney,muscle and ***,three SNPs(T27919A,T28139C and G28142A) and five haplotypes were *** with TTG was dominant with frequency of 69.1%.Chi-square test showed all populations were in Hardy-Weinberg equilibrium(P>0.05) at the three sites except Jiaxian cattle at G28142A site and Qinchuan cattle at T27919A *** analysis of combined sites showed that the individuals with TTGA genotype had significantly higher heart girth than those with TAGG genotype(P<0.05) and the animals with AAGA genotype had higher body weight than those with TAGG genotype(P<0.05) in T27919A-G28142A *** girth,abdominal circumference and body weight of individuals with TCAG genotype were exceedingly higher than those with TTGG(P<0.01),TTGA and TCGG(P<0.05) in T28139C-G28142A *** T27919A-T28139C site,the individuals of TCTA and TCTT genotype had significantly higher heart girth and lower height at hip cross than those with TTTA(P<0.05),and the body weight of TCAA and TCTT genotype individuals was higher than those with TTTA(P<0.05).In conclusion,these results provided evidence that the polymorphisms of RXRαgene were associated with growth traits and might apply to Chinese indigenous yellow cattle breeding program as a possible candidate for marker-assisted selection(MAS).

关键词：单核苷酸多态性组织表达谱中国黄牛生长性状基因型关联分析 Hardy-Weinberg平衡分子标记辅助选择

Study and practice of the controlling technique on heat-harm during the tunneling in Zhaolou mine

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Engineering Sciences 2008年第4期6卷 31-35页

作者： Liu Heqing Hao Xiaoli Wang yongjie Dai Huadong lin Qiguo College of Energy and Safety Engineering Hunan University of Science and Technology Xiangtan Hunan 411201 China Mining Key Laboratory in Hunan Province Xiangtan Hunan 411201 China The Center of Technology of Ventilation and Dusting in Mine Equipment Engineering Xiangtan Hunan 411201 China Heze Nenghua Co. Ltd of Yanzhou C0al Company Yuncheng Shandong 274700 China Xinlu Co. Ltd of Yangzhou Coal Company Zuocheng Shandong 273500 China

This paper proposes the cooling system type and cooling equipment type which are deep mine with high temperature during the construction,and presents auxiliary cooling measures making up duct temperature rise since compression and heat transfer temperature rise. The cooling system designed against Zhaolou mine's ground temperature and weather conditions,with its sprinkler room handling an average temperature difference up to 19.5～23.8 ℃,and the average enthalpy difference could reach 48.4～60.7 kJ/kg. At the same time,it gets a series of basic data used for mine construction during the cooling system design and equipment selection according to the measured results; using the analysis software Matlab,it obtains the change relations between the temperature of sprinkler room and fan export supply air temperature,wind casing temperature rise and fan export supply air temperature,working face temperature and supply air temperature,used for the mine cooling which has the similar conditions.

关键词： deep mine with high temperature mine development tunneling coaling

径向复合压电陶瓷超声换能器的径向振动特性研究

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声学学报 2007年第4期32卷 310-315页

作者：林书玉桑永杰田华陕西师范大学应用声学研究所西安710062

对径向复合压电陶瓷超声换能器的径向振动特性进行了分析。该换能器由一个厚度极化的压电陶瓷实心圆盘和一个金属圆环在半径方向复合而成。论文首先研究了压电陶瓷圆盘及金属圆环的径向振动,推出了其径向振动的机电等效电路。在此基础上... 详细信息

对径向复合压电陶瓷超声换能器的径向振动特性进行了分析。该换能器由一个厚度极化的压电陶瓷实心圆盘和一个金属圆环在半径方向复合而成。论文首先研究了压电陶瓷圆盘及金属圆环的径向振动,推出了其径向振动的机电等效电路。在此基础上,利用换能器的径向边界条件,得出了径向复合压电陶瓷换能器的机电等效电路及其共振频率方程。探讨了换能器的共振频率和有效机电耦合系数与其几何尺寸之间的依赖关系。设计并实际制作了一些径向复合超声换能器,对其径向共振及反共振频率进行了测试,并利用有限元法进行了数值模拟。研究表明,利用文中理论得出的换能器的共振频率与实测值及数值模拟结果基本符合。

关键词：压电陶瓷换能器超声换能器径向振动振动特性复合共振频率方程机电等效电路机电耦合系数