Highly Efficient A. T to G. C Base Editing by Cas9n- 3uided tRNA Adenosine Deaminase in Rice

作     者：Fang Yan Yongjie Kuang Bin Ren Jingwen Wang Dawei Zhang Honghui Lin Bing Yang Xueping Zhou Huanbin Zhou 

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricutural Sciences Beijing 100193 China Ministry of Education Key Laboratory of Bio-Resource and Eco-Environment College of Life Sciences Sichuan University Chengdu 610065 China College of Agronomy Henan University of Science and Technology Luoyang 471023 China Department of Genetics Development and Cell Biology Iowa State University Ames IA 50011 USA State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310058 China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2018年第11卷第4期

页      面：631-634页

核心收录：

学科分类：050302[文学-传播学] 0710[理学-生物学] 071010[理学-生物化学与分子生物学] 05[文学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0503[文学-新闻传播学] 

基　　金：This study was supported by grants from the National Key Research and Development Program of China (2017YFD0200900) and the Agricultural Science and Technology Innovation Program of The Chinese Academy of Agricultural Sciences to H.Z.  and a grant from the National Natural Science Foundation of China (31701780) to F.Y 

主　　题：CRISPR/Cas9 TadA base editing rice (Oryza sativa L.) 

摘      要：Dear Editor The newly developed CRISPR/Cas9-mediated base editing technology with cytosine deaminase is capable of precisely and efficiently introducing point mutations at the target genomic locus, which does not require double-stranded DNA breaks or any donor templates and thus exhibit a great potential for gene correction and genetic diversification in yeasts, plants, and mammalian and human cells （Komor et al., 2016; Nishida et al., 2016; Lu and Zhu, 2017; Ren et al., 2017）.