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Secretome profiling reveals temperature-dependent growth of Aspergillus fumigatus

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Science China(Life Sciences) 2018年第5期61卷 578-592页

作者： Dongyu Wang lili zhang Haiyue Zou Lushan Wang State Key Laboratory of Microbial Technology Shandong University

Aspergillus fumigatus is a ubiquitous opportunistic fungus. In this study, systematic analyses were carried out to study the temperature adaptability of A. fumigatus. A total of 241 glycoside hydrolases and 69 proteases in the secretome revealed the strong capability of A. fumigatus to degrade plant biomass and protein substrates. In total, 129 pathogenesis-related proteins detected in the secretome were strongly correlated with glycoside hydrolases and proteases. The variety and abundance of proteins remained at temperatures of 34°C–45°C. The percentage of endo-1,4-xylanase increased when the temperature was lowered to 20°C, while the percentage of cellobiohydrolase increased as temperature was increased, suggesting that the strain obtains carbon mainly by degrading xylan and cellulose, and the main types of proteases in the secretome were aminopeptidases and carboxypeptidases. Only half of the proteins were retained and their abundance declined to 9.7% at 55°C. The activities of the remaining β-glycosidases and proteases were merely 35% and 24%, respectively, when the secretome was treated at 60°C for 2 h. Therefore, temperatures >60°C restrict the growth of A. fumigatus.

关键词： Aspergillus fumigatus functional secretome temperature adaptability opportunistic pathogen saprophytic fungus

Diastereoselective Synthesis of Arylidene Bis（3-arylamino- acrylates） via One-pot Domino Reactions

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Chinese Journal of Chemistry 2013年第4期31卷 479-484页

作者： Zhenming Liu lili zhang Jing Sun Chaoguo Yan Analysis and Test Center Yangzhou University Yangzhou Jiangsu 225002 China College of Chemistry & Chemical Engineering Yangzhou University Yangzhou Jiangsu 225002 China

The functionalized arylidene bis（3-arylaminoacrylates） were efficiently prepared by FeCl3 catalyzed one-pot domino reactions of primary amines, methyl propiolate and aromatic aldehydes. When isatins were utilized under similar conditions, only 2-oxoindolinyl 3-arylaminoacrylates were obtained in moderate yields. ^1H NMR data and single crystal structures indicated that this reaction has high diastereoselectivity.

关键词： domino reaction electron-deficient alkyne acrylate isatin stereoselectivity

Diastereoselective Synthesis of Functionalized Tetrahydropyrimidin-2-thiones via ZnCl2 Promoted One-pot Reactions

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Chinese Journal of Chemistry 2014年第2期32卷 172-178页

作者： Zhenming Liu lili zhang Jing Sun Chaoguo Yan Analysis and Test Center Yangzhou UniversityYangzhouJiangsu 225002China College of Chemistry&Chemical Engineering Yangzhou UniversityYangzhouJiangsu 225002China

In the presence of zinc chloride,the in situ generatedβ-enamino ester from the reaction of morpholine,piperidine and pyrrolidine with methyl propiolate reacted,with aromatic aldehydes and thiourea in ethanol resulting in the functionalized tetrahydropyrimidin-2-thiones in satisfactory yields and with good *** aromatic aldehydes bearing electron-withdrawing group were used in the reaction,the 4-hydroxytetrahydropyrimidin-2-thione derivatives were obtained as the main product.

关键词： one-pot reaction domino reaction electron-deficient alkyne thiourea pyrimidin-2-thione

Paleogene marine deposition records of rifting and breakup of the South China Sea:An overview

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Science China Earth Sciences 2017年第12期60卷 2128-2140页

作者： LI QianYu WU GuoXuan zhang lili SHU Yu SHAO Lei State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China Shenzhen Branch of China National Offshore Oil Corporation Guangzhou 510240China

A compilation of available marine deposition data from offshore S-SE China reveals evidence of rifting and breakup of the South China Sea(SCS) during the Paleogene. Marine deposition started earlier in the Paleocene in the East China Sea(ECS)-Taiwan region before expanding southwestward into the SCS region in the middle Eocene. Our data indicate the existence of an elongated Paleogene China Sea in these areas stretching along the northeasterly structural belts, probably as part of the marginal western paleo-Pacific. The southwestward shift of marine influence in the middle Eocene was responding to a period of intensive rifting and subsidence in the SCS region, while the sea in the ECS-Taiwan region started to shrink and shoal after the late Eocene, likely associated with local breakup and initial spreading in the Taiwan-Taixinan Basin area. The accumulation of hemipelagic sediments at ODP 1148 and IODP U1435 from near the continent-ocean boundary and at many other shelf-slope sites was in response to a large-scale breakup 34 to 33 Ma ago, subsequently leading to the birth of the SCS in the Oligocene.

关键词： Paleogene South China Sea East China Sea Marine deposition Tectonic implication Breakup

Nutrient-patch contrast in relation to clonal integration, with special reference to Glechoma longituba

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Journal of Plant Ecology 2008年第1期1卷 75-77页

作者： lili zhang Weiming He State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China

Patch contrast,defined as the difference between patches,is among the basic traits of environmental heterogeneity(Kolasa and Pickett 1991;Stuefer 1996).Clonal plants,especially those with long spacers,usually experience certain patches with different nutrient-patch contrasts(de Kroon et al.2005;Stuefer 1996).Clonal integration has been recognized as a basic strategy,by which clonal plants can effectively cope with patchy resources and increase their fitness in such habitats(de Kroon et al.2005;zhang et al.2007).Glechoma longituba is distributed in a wide range of habitats(Wu and Chen 1974).Thus,it appears to be most likely that *** plants grow in habitats with different nutrient-patch *** that integration is highly dependent on source-sink relationships within a clone(Pitelka and Ashmun 1985),we hypothesize that increased nutrient-patch contrast might enhance clonal integration within a *** test this hypothesis,we conducted a garden experiment,in which *** was subjected to four levels of patch contrast.

关键词： Nutrient integration patch

Cell membrane-encapsulated nanoparticles for vaccines and immunotherapy

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Particuology 2022年第5期20卷 35-42页

作者： Wei Xiang Xue Liu lili zhang Chao Liu Gang Liu State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and Center for Molecular Imaging and Translational Medicine School of Public Health.Xiamen UniversityXiamen 361102China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics and National Institute of Diagnostics and Vaccine Development in Infectious Disease School of Public HealthXiamen UniversityXiamen 361102China ShenZhen Research Institute of Xiamen University.Shenzhen 518057 China

Functionalized nanoparticles are widely used in drug targeted delivery and immune microenvironment *** a new type of biomaterial,cell-derived carriers are receiving considerable attention in clinical translation owing to their desirable biocompatibility and lower ***,cell membrane-encapsulated nanoparticles can achieve specific biological effects from the benefit of their parent cell *** this review,we introduce the cell membrane coating technologies that are under extensive investigation,we also summarize various applications that have been developed for immunotherapy,and discuss the prospects in this *** further understanding and integration with advanced biotechnology methods,it is believed that the continuing development of cell membrane-encapsulated nanoparticles will achieve promotion in clinical application.

关键词： Cell membrane Nanoparticle Vaccine Immunotherapy

Space- and ground-based CO_2 measurements： A review

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Science China Earth Sciences 2016年第11期59卷 2089-2097页

作者： YUE TianXiang zhang lili ZHAO MingWei WANG YiFu John WILSON State Key Laboratory of Resources and Environment Information System Institute of Geographical Science and Natural Resources Research Beijing 100101 China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application Nanjing 210046 China Anhui Center for Collaborative Innovation in Geographical Information Integration and Application Chuzhou University Chuzhou 239012 China Spatial Sciences Institute University of Southern California Los Angles CA90089-0374 USA

The climate warming is mainly due to the increase in concentrations of anthropogenic greenhouse gases, of which CO_2 is the most important one responsible for radiative forcing of the climate. In order to reduce the great estimation uncertainty of atmospheric CO_2 concentrations, several CO_2-related satellites have been successfully launched and many future greenhouse gas monitoring missions are planned. In this paper, we review the development of CO_2 retrieval algorithms, spatial interpolation methods and ground observations. The main findings include: 1) current CO_2 retrieval algorithms only partially account for atmospheric scattering effects; 2) the accurate estimation of the vertical profile of greenhouse gas concentrations is a long-term challenge for remote sensing techniques; 3) ground-based observations are too sparse to accurately infer CO_2 concentrations on regional scales; and 4) accuracy is the primary challenge of satellite estimation of CO_2 concentrations. These findings, taken as a whole, point to the need to develop a high accuracy method for simulation of carbon sources and sinks on the basis of the fundamental theorem of Earth's surface modelling, which is able to efficiently fuse space- and ground-based measurements on the one hand and work with atmospheric transport models on the other hand.

关键词： Accuracy Carbon satellites Retrieval algorithms Space-and ground-based measurements HASM

Kinetic analysis and bacterium metabolization of α-pinene by a novel identified Pseudomonas ***

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Journal of Environmental Sciences 2012年第10期24卷 1806-1815页

作者： Zhuowei Cheng Pengfei Sun Yifeng Jiang lili zhang Jianmeng Chen Engineering Research Center of the Ministry of Education for Bioconversion and Biopurificationm Zhejiang University of Technology Hangzhou 310032 China. College of Biological and Environmental Engineering Zhejiang University of Technology Hangzhou 310032 China

Biodegradation has become a popular alternative remediation technology for its economic and ecological advantages. An aerobic bacterium （strain ZW） capable of degrading α-pinene was isolated from a biofilter by a selective enrichment. Based on the 16S rRNA gene analysis and physiochemical properties, this strain was identified as Pseudomonas veronii. Under the optimized condition achieved by the response surface methodology （RSM）, as well as pH 6.82, temperature 26.3℃ and NaC1 concentration 1.36%, almost 100% α-pinene could be removed within 45 hr. Enzymatic biodegradation by the crude intracellular enzyme could be described well by the Michaelis-Menten model in which the maximum degradation rate Vraax and the half-saturation constant Km were calculated to be 0.431 mmol/（***） and 0.169 mmol/L, respectively. Activity assay of catechol suggested that the strain ZW possessed a catechol- 1,2-dioxygenase and could decompose benzene-ring through ortho ring cleavage. Based on the identified intermediates by GC/MS, a new metabolic pathway was proposed, in which the final metabolites were some simpler organic and inorganic compounds. The present work demonstrated that the strain ZW would have a great application prospect for the remediation of α-pinene-contaminated environment.

关键词： Pseudomonas veronii ct-pinene growth conditions kinetics analysis metabolic pathway

A luminescent metal-organic framework with an open cubic cage and eight-coordinate cadmium nodes

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Science China Chemistry 2010年第10期53卷 2079-2082页

作者： WANG ChuanFeng zhang ZhengXing zhang lili LI FuYou ZHOU XiGeng Department of Chemistry Fudan UniversityShanghai 200433China Department of Chemistry Taizhou CollegeLinhai 317000China

Self-assembly of cadmium ions and the rigid bridging ligand 4,4′-methylenebis(3-hydroxy-2-naphthoic acid) (pamoic acid, H4PA) leads to a one-dimensional metal-organic framework with open cube-like M2(H2PA)2 cages wit... 详细信息

关键词： framework eight-coordinate cadmium cubic cage fluorescence pamoic acid