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Science China Chemistry 2009年第12期52卷 2264-2268页

作者： liu lihong CHEN ZiLin Department of Pharmaceutical Analysis College of PharmacyWuhan UniversityWuhan 430072China

Peptidomics has emerged as a new discipline in recent years. Mass spectrometry (MS) is the most universal and efficient tool for structure identification of proteins and peptides. However,there is a limitation for the identification of peptides with the same amino acid composition but different se-quences because these peptides have identical mass spectra of molecular ions. This paper presents a high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method for the separation of small molecular peptides with the same amino acid composition but dif-ferent sequences. Two tripeptides of Gly-Ser-Phe and Gly-Phe-Ser were used as a model sample. The separation behavior has been investigated and the separation conditions have been optimized. Under the optimum conditions,good repeatability was achieved. The developed method could provide a helpful reference for the separation of other peptides with the same amino acid composition but different sequences in the study of proteomics and peptidomics.

关键词： reversed-phase chromatography mass spectrometry peptides amino acid

Cetyltrimethylammonium Bromide（CTAB）-Ionic Liquid Composite Modified Electrode for Sensitive Cyclic Voltammetric Determination of Bisphenol A

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Chemical Research in Chinese Universities 2016年第6期32卷 992-995页

作者： liu lihong WANG Chunfeng ZHUO Kelei Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals Key Laboratory of Green Chemical Media and Reactions Ministry of EducationSchool of Chemistry and Chemical Engineering Henan Normal University Xinxiang 453007 P. R. China

A sensitive electrochemical sensor for determining bisphenol A（BPA） was designed. The sensor was aglassy carbon electrode modified with the surfactant cetyltrimethylammonium bromide and the ionic liquid1-decyl-3-methylimidazolium tetrafluoroborate. The ability of the new sensor to measure BPA was investigated incyclic voltanmletry experiments. Under optimized conditions, the sensor gave a linear response range for BPA of2.19×10^-7 -3.28× 10^-5 mol/L and a detection limit of 7.31×10^8 mol/L（S/N=3） BPA could be determined with a lowerdetection limit, a wider linear range, and more sensitivity using the sensor than using other electrochemical sensors orhigh performance liquid chromatography with UV detection. The new sensor was used to determine BPA in tap waterwith recoveries of 97.5%--98.7% and a relative standard deviation 〈2.9%. The results show that the sensor can beused to determine trace BPA concentrations in tap water.

关键词： Bisphenol A Ionic liquid Surfactant Electrochemical sensor

Fe3O4 encapsulated mesoporous silica nanospheres with tunable size and large void pore

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Frontiers of Chemical Science and Engineering 2014年第1期8卷 114-122页

作者： Tingting liu lihong liu Jian liu Shaomin liu Shi Zhang QIAO Department of Chemical Engineering Curtin University Perth WA 6845 Australia School of Chemical Engineering The University of Adelaide SA5005 Australia

Magnetic Fe3O4 and mesoporous silica core- shell nanospheres with tunable size from 110-800 nm were synthesized via a one step self-assembly method. The morphological, structural, textural, and magnetic proper- ties were well-characterized by scanning electron micro- scopy, transmission electron microscopy, X-ray diffraction, N2 adsorption-desorption and magnetometer. These nanocomposites, which possess high surface area, large pore volume and well-defined pore size, exhibit two dimensional hexagonal （P6mm） mesostructures. Interest- ingly, magnetic core and mesoporous silica shell nano- composites with large void pore （20 nm） on the shell were generated by increasing the ratio of ethanol/water. Additionally, the obtained nanocomposites combined magnetization response and large void pore, implying the possibility of applications in drug/gene targeting delivery. The cell internalization capacity of NH2-functionalized nanocomposites in the case of cancer cells （HeLa cells） was exemplified to demonstrate their nano-medicine application.

关键词： mesoporous silicas magnetic nanoparticles,core-shell nanoparticles cell uptake

Estimation of bioaccessibility and potential human health risk of mercury in Chinese patent medicines

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Journal of Environmental Sciences 2016年第1期28卷 37-44页

作者： lihong liu Yu Zhang Zhaojun Yun Bin He Guibin Jiang State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Centre for Eco-Environmental Sciences Chinese Academy of Sciences Wangjing Hospital China Academy of Chinese Medical Sciences

Mercury（Hg）, mainly in cinnabar species, has been used in medicine for thousands of years in China, and worldwide concern has been raised on its toxicity. In this work, the amount of bioaccessible mercury in 16 Chinese patent medicines（CPMs） was measured by using an in vitro simulated digestion system, consisting of simulated gastric and intestinal fluid, to investigate the bioavailability of mercury in CPMs and evaluate its potential risk to human health. Total mercury and mercury in the gastrointestinal extracts were measured by inductively coupled plasma mass spectrometry（ICP-MS）. The levels of total Hg in 16 CPMs ranged from not detected to 11.89 mg/g, with a mean value of 1.13 mg/g, while the extractable Hg ranged from not detected to 4.37 μg/g, with a mean value of 0.42 μg/*** bioaccessibility varied significantly in the investigated CPMs, depending on the ingredient. Compared to the CPMs without cinnabar（2.5%–30.9%）, the percentage of mercury in the gastrointestinal supernatants for CPMs with cinnabar was quite a bit lower（0.037%）. By comparing with the Food and Agricultural Organization/World Health Organization Joint Expert Committee on Food Additives（FAO/WHO） safety guideline, the average daily intake dose（ADD） of Hg in the medicines was then calculated to access the risk of mercury to human health from taking CPMs.

关键词： Bioaccessibility Mercury Chinese patent medicine Simulated digestion extraction

A New Example of Retrograde Solubility Model for Carbonate Rocks

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Acta Geologica Sinica(English Edition) 2017年第3期91卷 1145-1146页

作者： liu lihong WANG Chunlian WANG Daming WANG Haida Oil & Gas Survey China Geological Survey Oil and Gas Research Center School of Earth and Space SciencesPeking University MLR Key Laboratory of Metallogeny and Mineral Assessment Institute of Mineral ResourcesChinese Academy of Geological Sciences China Geological Survey

Objective The dissolution and precipitation of carbonate during burial diagenetic process controls the reservoir property in deep buried strata. The geological process related with it has become a research focus during recent years. The most important dissolution fluids to carbonates are probably H2S and CO2 as byproducts of sulfate reduction in deep-buried setting with sulfate minerals, but carbonates are more soluble in relatively low temperature, which is the so-called retrograde solubility. Several geological processes can result in the decrease of temperature, including the upward migration of thermal fluids and tectonic uplift.

关键词： of on with well A New Example of Retrograde Solubility Model for Carbonate Rocks for

On (α,β)-Metrics with Reversible Geodesics

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Journal of Mathematical Research with Applications 2016年第5期36卷 568-574页

作者： lihong liu Guangzu CHEN School of Science East China Jiao Tong University

In this paper,we get necessary and sufficient conditions for a Finsler space endowed with an（α,β）-metric where its geodesic coefficients Gi（x,y） and the reverse of geodesic coefficients Gi（x,-y） have the same ... 详细信息

关键词： (α,β)-metric geodesic coefficient reversible geodesic Douglas curvature

Enhanced Performance of Denitrifying Sulfide Removal Process by 1,2-Naphthoquinone-4-Sulphonate

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China Petroleum Processing & Petrochemical Technology 2016年第1期18卷 22-28页

作者： liu Chunshuang Han Kang Zhao Dongfeng Guo Yadonag liu lihong liu Fang Zhao Chaocheng College of Chemical Engineering China University of PetroleumQingdao School of Earth Sciences Northeast Petroleum University

The denitrifying sulfide removal(DSR) process with bio-granules comprising both heterotrophic and autotrophic denitrifiers can simultaneously convert nitrate, sulfide and acetate species into di-nitrogen gas, elemental sulfur and carbon dioxide, respectively, at high loading rates. This study has determined that the reaction rate of sulfide oxidized into sulfur could be enhanced in the presence of 1,2-naphthoquinone-4-sulphonate(NQS). The presence of NQS mitigated the inhibition effects of sulfide species on denitrification. Furthermore, the reaction rates of nitrate and acetate to nitrogen gas and CO_2, respectively, were also promoted in the presence of NQS, thereby enhancing the performance of DSR granules. The advantages and disadvantages of applying the NQS-DSR process are discussed.

关键词： denitrifying sulfide removal NQS inhibition bio-granules

The influence of dihydropyridines calcium antagonists on 5-HT-induced intracellular calcium signal

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Science China Chemistry 2007年第4期50卷 562-567页

作者： WANG ShuMin GUAN HuaShi FANG Yi MA Ping SUN JianZi liu lihong Marine Drug and Food Institute Ocean University of ChinaQingdao 266003China Beijing Tongren Hospital Capital University of Medical SciencesBeijing 100730China Chinese PLA General Hospital Beijing 100853China Chinese PLA General Hospital of Rocket Forces Beijing 100088China

Confocal laser scanning microscopy (CLSM) was applied to detect the intracellular [Ca2+] variety of fluorescent intension, with Fluo-3/AM fluorescence loaded in SFSMC. The results show that 10 μmol/L Lacidipine can reduce the frequence which 10 μmol/L 5-HT induced [Ca2+] spark in SFSMC of calcium over loading to 50%, and amplitude to 50% or so. We can draw a conclusion that dihydropyridines cal-cium antagonists lacidipine can antagonize the release of intracellular [Ca2+] which 5-HT-induced in dose dependent manner.

关键词： lacidipine calcium sparks stomach fundus smooth muscle cells confocal laser scanning microscopy

Development of a cell-permeable adenine-derived probe for capture of nucleotide-binding proteins in living cells

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Chinese Chemical Letters 2024年第3期35卷 243-246页

作者： lihong liu Rui Chen Gang Xue Chenzhou Hao Weizhi Weng Zhengying Pan State Key Laboratory of Chemical Oncogenomics School of Chemical Biology and BiotechnologyPeking University Shenzhen Graduate SchoolShenzhen 518055China

Adenine is an essential building block of genetic material and a range of *** probes containing an adenine moiety have been used in kinase profiling studies in cell *** we report that adenine-derived small-molecule probes with an activated ester reactive group can covalently modify a conserved lysine residue of protein kinases and capture a number of nucleotide-binding proteins within living cells.

关键词： Adenine-derived probe Cell-permeable Lysine residue Protein kinases Nucleotide-binding proteins

B-Valued Dyadic Derivative

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Wuhan University Journal of Natural Sciences 2007年第6期12卷 961-964页

作者： ZHANG Chuanzhou CHEN lihong,liu Peide School of Mathematics and Statistics Wuhan UniversityWuhan 430072 Hubei China

The principles of the new maximal operator H＊ we defined are discussed. We prove that it is bounded from martingale Hardy-Lorentz L^Xp.q[0,1） to the Lorentz L^Xp.q[0,1） for 1/2〈 p〈∞, 0〈~ q ≤ ∞, where X is any Banach space. When the Banach space X has the RN property, the sequence dnHnf converges to f a.e. Meanwhile the convergence in L^Xp norm for 1≤p〈∞ is a consequence of that the family functions K （n∈N） is an approximate identity.

关键词： martingale Hardy space dyadic derivative dyadic integral