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Sugarcane transcription factor ScWRKY4 negatively regulates resistance to pathogen infection through the JA signaling pathway

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The Crop Journal 2024年第1期12卷 164-176页

作者： Dongjiao Wang Wei Wang Shoujian Zang Liqian Qin yanlan liang Peixia Lin Yachun Su Youxiong Que Key Laboratory of Sugarcane Biology and Genetic Breeding Ministry of Agriculture and Rural AffairsKey Laboratory of GeneticsBreeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina National Key Laboratory for Tropical Crop Breeding Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesSanya 572024HainanChina

WRKY transcription factors,transcriptional regulators unique to plants,play an important role in defense response to pathogen ***,the resistance mechanisms of WRKY genes in sugarcane remain *** the present study,gene ontology(GO)enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in defense *** identified gene ScWRKY4,a classⅡc member of the WRKY gene family,in sugarcane cultivar *** gene was induced by salicylic acid(SA)and methyl jasmonate(MeJA)***,expression of ScWRKY4 was down-regulated in smut-resistant sugarcane cultivars but up-regulated in smutsusceptible sugarcane cultivars infected with Sporisorium ***,stable overexpression of the ScWRKY4 gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused down-regulated expression of immune marker-related *** analysis indicated suppressed expression of most JAZ genes in the signal transduction ***4 interacted with ScJAZ13 to repress its *** thus hypothesized that the ScWRKY4 gene was involved in the regulatory network of plant disease resistance,most likely through the JA signaling *** present study depicting the molecular involvement of ScWRKY4 in sugarcane disease resistance lays a foundation for future investigation.

关键词： Disease resistance Expression profile Transcriptome analysis WRKY transcription factors

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A Transcription Factor ScWRKY4 in Sugarcane Negatively Regulates the Resistance to Pathogen Infection

A Transcription Factor ScWRKY4 in Sugarcane Negatively Regul...

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中国植物病理学会2023年学术年会

作者： WANG Dongjiao WANG Wei ZANG Shoujian QIN Liqian LIN Peixia liang yanlan SU Yachun QUE Youxiong

WRKY transcription factor, the transcriptional regulators unique to plants, plays an important role in plant defense response to pathogen ***, research on the disease resistance mechanism of WRKY gene in sugarcane is still ***, GO enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in the process of defence ***, we identified a ScWRKYA gene, a member of class lie of the WRKY gene family, from the sugarcane cultivar *** upstream promoter contained multiple cis-acting elements,which were associated with stress, growth and development and hormone *** gene could be induced by the stresses of salicylic acid( SA) and methyl jasmonate( MeJA).The expression of the ScWRKY4 gene was down-regulated in smut-resistant sugarcane cultivars and up-regulated in smut-susceptible sugarcane cultivars under Sporisorium scitamineum *** overexpression of the ScWRKYA gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused the down-regulated expression of immune marker-related *** analysis indicated that, at 0 and 2 d after inoculation with *** ***, tobacco plants stably overexpressing the ScWRKYA gene were significantly enriched for differentially expressed genes in plant hormone signal transduction pathway,plant-pathogen interaction pathway and MAPK signaling *** is interesting that, the expression of most JAZ genes was suppressed in plant signal transduction *** addition, ScWRKY4 could interact with ScJAZ13 and repress the expression of *** above results confirmed that the ScWRKYA gene was involved in the regulatory network of plant disease resistance through the JA signaling pathway, and laid the foundation for the subsequent investigation on the molecular mechanism of the ScWRKYA gene involved in sugarcane disease resistance.

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Sugarcane ShNPR2 Gene and Its Promoter Positively Respond to Pathogen Infection and Exogenous Hormone Stresses

Sugarcane ShNPR2 Gene and Its Promoter Positively Respond to...

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中国植物病理学会2023年学术年会

作者： ZANG Shoujian HUANG Tingchen WU Guran WANG Dongjiao QIN Liqian liang yanlan LIN Peixia SU Yachun QUE Youxiong

Nonexpressor of pathogenesis-related genes 1(NPR 1),as a key regulatory factor in the plant salicylic acid(SA) signal transduction pathway,plays a core regulatory role in plant systemic disease *** the present study,a total of 18 SsNPR and 35 SoNPR genes were identified from sugarcane wild species Saccharum spontaneum and tropical species ***,*** analysis demonstrated that NPR proteins could be clustered into three *** analysis revealed a close homology between NPR genes in *** and *** data analysis indicated that SsNPR genes were tissue-specifically expressed and differentially expressed in different genotypes of sugarcane interacting with smut fungus.A differentially expressed gene,ShNPR2,was then cloned from the sugarcane cultivar ***2 was found to be a nuclear protein without transcriptional self-activation activity,constitutively expressed in different tissues of ROC22,and response to the stresses of methyl jasmonate(MeJA),abscisic acid(ABA),and *** is worth noting that transient overexpression of the ShNPR2 gene in Nicotiana benthamiana showed a higher sensitivity to the infection of Ralstonia solauacearum and Fusarium solani ***,and this regulatory effect was closely related to the expression changer of genes related to hypersensitive reaction(HR),SA,and ethylene(ET) synthesis *** addition,the promoter of the ShNPR2 gene,pro-ShNPR2,was isolated from ROC22 and found to have transcriptional ***,the activity of pro-ShNPR2 was upregulated by exogenous treatments with MeJA,ABA,and *** present study provided a theoretical and practical basis for comprehensively understanding the sequence characteristics,expression status,and disease resistance function of sugarcane NPR genes.

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基于层次分析法的豫北地区雨水花园植物综合评价

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水土保持通报 2019年第1期39卷 120-124页

作者：梁彦兰陈晓霞王昭娜安阳工学院土木与建筑工程学院河南安阳455000

[目的]研究豫北地区雨水花园植物的筛选,为雨水花园和海绵城市的建设提供理论支撑。[方法]对国家级海绵城市鹤壁市和河南省省级海绵城市安阳市境内的典型雨水花园植物进行研究,采用AHP层次分析法对29种雨水花园植物进行综合评价,从3个... 详细信息

[目的]研究豫北地区雨水花园植物的筛选,为雨水花园和海绵城市的建设提供理论支撑。[方法]对国家级海绵城市鹤壁市和河南省省级海绵城市安阳市境内的典型雨水花园植物进行研究,采用AHP层次分析法对29种雨水花园植物进行综合评价,从3个准则层和9个指标层构建植物评价体系。[结果]指标层中的抗旱性、抗湿性、绿化美化特性、去污降污能力4个指标对于雨水花园植物的选择具有重要影响。千屈菜、红蓼、东方狼尾草、鸢尾、细叶芒、花叶芒6种植物综合评价等级为1级;再力花、常夏石竹、八宝景天、美人蕉、金鸡菊、马鞭草、大丽花7种植物综合评价等级为4级;香彩雀、紫叶狼尾草、蜀葵等16种植物综合评价等级为2—3级。[结论]综合评价值为1级的植物是豫北地区建设雨水花园的首选植物;综合评价等级为4级的植物是雨水花园慎重选择的植物材料;综合评价等级为2—3级的植物,选择合适的区域如蓄水区、缓冲区、边缘区进行雨水花园植物的种植。

关键词：层次分析法雨水花园植物选择评价

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城市绿地系统规划课程教学改革探讨

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安阳工学院学报 2016年第4期15卷 109-111页

作者：梁彦兰王保民安阳工学院土木与建筑工程学院河南安阳455000

城市绿地系统规划课程是园林专业、风景园林专业、城市规划专业的专业必修课程,在课程体系中具有重要作用。在促进城市可持续发展和生态宜居城市背景下,城市绿地系统规划对于改善城市生态环境,完善城市绿色基础设施方面具有重要意义。... 详细信息

城市绿地系统规划课程是园林专业、风景园林专业、城市规划专业的专业必修课程,在课程体系中具有重要作用。在促进城市可持续发展和生态宜居城市背景下,城市绿地系统规划对于改善城市生态环境,完善城市绿色基础设施方面具有重要意义。本文通过对应用型高校城市绿地系统规划课程教学中存在的问题进行分析研究,对课程进行教学改革进行了探讨,以适应应用型高校课程建设发展需求。

关键词：城市绿地系统规划教学改革

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The dopaminergic system and Alzheimer's disease

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Neural Regeneration Research 2025年第9期20卷 2495-2512页

作者： Yuhan Zhang Yuan liang Yixue Gu International Medical College Chongqing Medical UniversityChongqingChina College of Pharmacy Chongqing Medical UniversityChongqingChina Department of Neurology The First Affiliated Hospital of Chongqing Medical UniversityChongqing Key Laboratory of NeurologyChongqingChina

Alzheimer's disease is a common neurodegenerative disorder in older *** its prevalence,its pathogenesis remains unclea *** addition to the most widely accepted causes,which in clude excessive amyloid-beta aggregation,tau hyperphosphorylation,and deficiency of the neurotransmitter acetylcholine,numerous studies have shown that the dopaminergic system is also closely associated with the occurrence and development of this *** is a crucial catecholaminergic neurotransmitter in the human ***-associated treatments,such as drugs that target dopamine receptor D and dopamine analogs,can improve cognitive function and alleviate psychiatric symptoms as well as ameliorate other clinical *** r,therapeutics targeting the dopaminergic system are associated with various adverse reactions,such as addiction and exacerbation of cognitive *** review summarizes the role of the dopaminergic system in the pathology of Alzheimer's disease,focusing on currently available dopamine-based therapies for this disorder and the common side effects associated with dopamine-related *** aim of this review is to provide insights into the potential connections between the dopaminergic system and Alzheimer's disease,thus helping to clarify the mechanisms underlying the condition and exploring more effective therapeutic options.

关键词： adverse drug reaction Alzheimer's disease catecholamine dopamine receptor dopamine receptor heterodimers dopaminergic system neurodegenerative disease neurotransmitter signaling pathways traditional Chinese medicine treatment

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广西融水县南刀坳锡多金属矿地球化学特征与成矿过程分析

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现代矿业 2021年第7期37卷 26-29,69页

作者：梁燕兰戴昱吴庆杰广西壮族自治区区域地质调查研究院广西壮族自治区桂林市541003

广西融水县南刀坳锡多金属矿位于江南造山带南缘,三防岩体的西南外接触带上,先后发现了多个大中型锡多金属矿床。通过岩石地球化学、矿石元素分析和元素相关性分析等方法,探讨锡矿的成矿物质来源和成矿过程。通过对地球化学特征分析认为... 详细信息

广西融水县南刀坳锡多金属矿位于江南造山带南缘,三防岩体的西南外接触带上,先后发现了多个大中型锡多金属矿床。通过岩石地球化学、矿石元素分析和元素相关性分析等方法,探讨锡矿的成矿物质来源和成矿过程。通过对地球化学特征分析认为,该矿的Sn等成矿物质主要来自三防岩体,Pb和Zn等金属元素可能来自地层萃取。金属元素在岩浆演化过程中从熔浆中分离出来,通过隐伏岩体顶部分馏进入地层,提供了区域锡矿成矿的物质来源。成矿过程基本可以分为4个阶段:第一阶段是高温Sn热液从三防岩体侵位过程中分离、结晶、富集;第二阶段是锡矿的剪切改造;第三阶段是含矿热液携带碎裂锡石就位及锡石重结晶;第四阶段为成矿后热液改造叠加。

关键词：三防岩体地球化学特征成矿物质来源成矿过程

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林州市核桃举肢蛾的发生规律与化学防治

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河南农业科学 2015年第1期44卷 86-89页

作者：张坤朋王相宏王景顺梁彦兰王峰付亮安阳工学院河南安阳455000 林州市森林病虫防治检疫站河南林州456550 北京市怀柔区林木病虫防治检疫站北京101400

为明确核桃举肢蛾在林州市的发生规律,筛选防治核桃举肢蛾的最佳药物及浓度,进行了核桃举肢蛾生活史的系统观察及树冠喷药防治试验。结果表明,核桃举肢蛾在林州市1 a发生2代,第一代成虫发生高峰在4月中下旬,幼虫蛀果高峰为4月下旬,第二... 详细信息

为明确核桃举肢蛾在林州市的发生规律,筛选防治核桃举肢蛾的最佳药物及浓度,进行了核桃举肢蛾生活史的系统观察及树冠喷药防治试验。结果表明,核桃举肢蛾在林州市1 a发生2代,第一代成虫发生高峰在4月中下旬,幼虫蛀果高峰为4月下旬,第二代成虫发生高峰在6月中下旬,幼虫蛀果高峰在7月上旬。采用毒死蜱1 000倍液、苦参碱500倍液和乙酰甲胺磷1 000倍液进行树冠喷雾,从核桃举肢蛾成虫发生高峰期开始,第一代成虫期喷1遍药,第二代成虫期喷2遍药,2遍间隔10 d,防治效果明显,防效在82.9%~89.0%,可以在生产中推广应用。

关键词：核桃举肢蛾发生规律化学防治防治适期

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安阳市园林绿化植物调查规划

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安阳工学院学报 2008年第4期7卷 22-24页

作者：梁彦兰李亚藏安阳工学院河南安阳455000

通过调查安阳市区园林绿化现状以及园林植物的种类、数量、分布,并进行归纳总结,提出了安阳市园林绿化存在的问题,以及今后安阳市园林绿化与绿化系统建设对策,为创造生态和谐的市区环境提供理论基础。

关键词：安阳园林植物调查规划

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Superhydrophobic and corrosion-resistant siloxane-modified MgAl-LDHs coatings on magnesium alloy prepared under mild conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 450-463页

作者： Wenxi Zhang Zhangzelong Zhuo Dan Xu liang Wu Zhihui Xie Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province College of Chemistry and Chemical EngineeringChina West Normal UniversityNanchong 637002China College of Materials Science and Engineering/National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400044China

We have developed a superhydrophobic and corrosion-resistant LDH-W/PFDTMS composite coating on the surface of Mg *** composite comprised a tungstate-intercalated(LDH-W)underlayer that was grown at low temperature(relative to hydrothermal reaction conditions)under atmospheric pressure and an outer polysiloxane layer created from a solution containing perfluorodecyltrimethoxysilane(PFDTMS)using a simple immersion *** successful intercalation of tungstate into the LDH phase and the following formation of the polysiloxane layer were confirmed through X-ray diffraction(XRD),Fourier transform infrared(FTIR)spectroscopy,and X-ray photoelectron spectroscopy(XPS).The corrosion resistance of the LDH-W film,both before and after the PFDTMS modification,was evaluated using electrochemical impedance spectroscopy(EIS),Tafel curves,and immersion *** results showed that Mg coated with LDH-W/PFDTMS exhibited significantly enhanced corrosion protection compared to the unmodified LDHW film,with no apparent signs of corrosion after exposure to 3.5wt%NaCl solution for 15 ***,the LDH-W/PFDTMS coating demonstrated superior superhydrophobicity and self-cleaning properties against water and several common beverages,as confirmed by static contact angle and water-repellency *** results offer valuable insights into preparing superhydrophobic and corrosion-resistant LDH-based composite coatings on Mg alloy surfaces under relatively mild reaction conditions.

关键词： Mg alloy corrosion coating layered double hydroxide

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