A Transcription Factor ScWRKY4 in Sugarcane Negatively Regulates the Resistance to Pathogen Infection
会议名称：《中国植物病理学会2023年学术年会》
会议日期：2023年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
摘 要：WRKY transcription factor, the transcriptional regulators unique to plants, plays an important role in plant defense response to pathogen ***, research on the disease resistance mechanism of WRKY gene in sugarcane is still ***, GO enrichment analysis revealed that WRKY gene family in sugarcane was extensively involved in the response to biotic stress and in the process of defence ***, we identified a ScWRKYA gene, a member of class lie of the WRKY gene family, from the sugarcane cultivar *** upstream promoter contained multiple cis-acting elements,which were associated with stress, growth and development and hormone *** gene could be induced by the stresses of salicylic acid( SA) and methyl jasmonate( MeJA).The expression of the ScWRKY4 gene was down-regulated in smut-resistant sugarcane cultivars and up-regulated in smut-susceptible sugarcane cultivars under Sporisorium scitamineum *** overexpression of the ScWRKYA gene in Nicotiana benthamiana enhanced susceptibility to Fusarium solani *** and caused the down-regulated expression of immune marker-related *** analysis indicated that, at 0 and 2 d after inoculation with *** ***, tobacco plants stably overexpressing the ScWRKYA gene were significantly enriched for differentially expressed genes in plant hormone signal transduction pathway,plant-pathogen interaction pathway and MAPK signaling *** is interesting that, the expression of most JAZ genes was suppressed in plant signal transduction *** addition, ScWRKY4 could interact with ScJAZ13 and repress the expression of *** above results confirmed that the ScWRKYA gene was involved in the regulatory network of plant disease resistance through the JA signaling pathway, and laid the foundation for the subsequent investigation on the molecular mechanism of the ScWRKYA gene involved in sugarcane disease resistance.