检索结果-南通市图书馆

Switching On/Off of Guide RNA by Photoinduced Strand Displacement for Functional Control of CRISPR/Cas9

CCS Chemistry 2024年第5期6卷 1338-1351页

作者： Yang wang Chao-Qun Wu Qiu-Long Zhang Ming Shao Yan Liu liang-liang wang Zun-Yun wang Juan Du liang Xu Central Laboratory Department of the Second Affiliated Hospital School of MedicineThe Chinese University of Hong KongShenzhen&Longgang District People’s Hospital of ShenzhenShenzhen 518172 Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060 MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275 School of Pharmacy and Medical Technology Putian UniversityPutian 351100

Functional control of CRISPR/Cas9 is essential for precise gene *** engineering of guide RNA(gRNA)provides diverse approaches for conditional control of CRISPR/Cas9 function with a variety of chemical reactive ***,previous investigations into chemically engineering gRNA only unidirectionally regulated the CRISPR/Cas9 function via stimuli-induced caging/decaging ***,we propose a combinatory strategy to engineer the dynamics of gRNA in which photocontrolled strand-displacement reactions coupled with sequence designs of gRNA can achieve lightinduced switching-on/off control of CRISPR/Cas9 *** analysis and cellular gene regulation indicate this approach is capable of both activating and deactivating CRISPR/Cas9 activities using light ***,photocontrolled multiplex modulations of gene expression for opposite regulatory effects have also been achieved simultaneously under the same cellular *** work establishes an essential principle for construction of stimuli-induced switching-on/off modulations of gRNA that can greatly enrich the versatility of conditional control for a variety of CRISPR/Cas9-based applications.

关键词： modified oligonucleotides gene manipulation optical control CRISPR/Cas9 strand displacement

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Digitization of Astronomical Photographic Plates of China and Astrometric Measurement of Single-exposure Plates

引用

Research in Astronomy and Astrophysics 2024年第5期24卷 103-116页

作者：商正君于涌王亮亮杨美婷杨静沈世银刘敏徐权峰崔辰州樊东卫唐正宏赵建海 Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai 200030China University of Chinese Academy of Sciences Beijing 100049China Key Lab for Astrophysics Shanghai200234China National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China National Astronomical Data Center Chinese Academy of SciencesBeijing 100101China

From the mid-19th century to the end of the 20th century, photographic plates served as the primary detectors for astronomical observations. Astronomical photographic observations in China began in 1901, and over a century, a total of approximately 30,000 astronomical photographic plates were captured. These historical plates play an irreplaceable role in conducting long-term, time-domain astronomical research. To preserve and explore these valuable original astronomical observational data, Shanghai Astronomical Observatory has organized the transportation of plates, taken during nighttime observations from various stations across the country, to the Sheshan Plate Archive for centralized preservation. For the first time, plate information statistics were calculated. On this basis, the plates were cleaned and digitally scanned, and finally digitized images were acquired for 29,314 plates. In this study, using Gaia DR2 as the reference star catalog, astrometric processing was carried out successfully on 15,696 single-exposure plates, including object extraction, stellar identification,and plate model computation. As a result, for long focal length telescopes, such as the 40 cm double-tube refractor telescope, the 1.56 m reflector telescope at Shanghai Astronomical Observatory, and the 1m reflecting telescope at Yunnan Astronomical Observatory, the astrometric accuracy obtained for their plates is approximately 0."1–0."3. The distribution of astrometric accuracy for medium and short focal length telescopes ranges from 0."3 to 1."0. The relevant data of this batch of plates, including digitized images and a stellar catalog of the plates, are archived and released by the National Astronomical Data Center. Users can access and download plate data based on keywords such as station, telescope, observation year, and observed celestial coordinates.

关键词： methods data analysis-techniques image processing-astronomical databases miscellaneous-astrometry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of magnesium addition on inclusions in H13 die steel

引用

International Journal of Minerals,Metallurgy and Materials 2014年第11期21卷 1062-1067页

作者： Zheng Wu Jing Li Cheng-bin Shi liang-liang wang State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing

The effect of magnesium addition on the number, morphology, composition, size, and density of inclusions in H13 die steel was studied. The results show that the total oxygen content in the steel can be significantly decreased to 0.0008wt%. Al2O3 and Mn S inclusions are changed into nearly spherical Mg O·Al2O3 spinel and spherical Mg O·Mg S inclusions, respectively. The number of inclusions larger than 1 μm decreases and the number of inclusions smaller than 1 μm increases with increasing magnesium content. V（N,C） precipitates around Mg O·Al2O3 and Mg O·Mg S inclusions during solidification of liquid steel. The densities of Mg O·Al2O3 spinel inclusions are lower than that of alumina inclusions. With increasing magnesium content in the Mg-containing inclusions, the density of inclusions decreases, leading to the improvement of inclusion removal efficiency.

关键词： die steel inclusions magnesium deoxidation thermodynamics

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Quasilocal Energy for Static Charged Black Holes in String Theory

引用

Communications in Theoretical Physics 2001年第7期36卷 125-128页

作者： wang Shi-liang JING Ji-liang wang Yong-Jiu Institute of Physics and Physics Department Hunan Normal University Changsha 410081 China Institute of Physics and Physics Department Hunan Normal University Changsha 410081 China Department of Astronomy and Applied Physics University of Science and Technology of China Hefei 230026 China Institute of Physics and Physics Department Hunan Normal University Changsha 410081 China

The Brown-York quasilocal energies of some static charged dilaton black holes are calculated, and then the validity of Martinez's conjecture is explored in string theory. It is shown that the energy is positive and monotonically decreases to the ADM mass at spatial infinity, and the conjecture that the Brown-York quasilocal energy at the outer horizon of black hole reduces to twice of its irreducible mass is still applicable for the static charged black holes in string theory. The result is different from Bose-Naing's one.

关键词： quasilocal energy, black hole, string theory

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Derived Born cross sections of e^+e^-annihilation into open charm mesons from CLEO-c measurements

引用

Chinese Physics C 2018年第4期42卷 69-75页

作者： Xiang-Kun Dong liang-liang wang Chang-Zheng Yuan University of Chinese Academy of Sciences Beijing 100049 China Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China

The exclusive Born cross sections of the production of D0, D＋ and Ds＋ mesons in e＋e- annihilation at 13 energy points between 3.970 and 4.260 GeV are obtained by applying corrections for initial state radiation and vacuum polarization to the observed cross sections measured by the CLEO-c experiment. Both the statistical and the systematic uncertainties for the obtained Born cross sections are estimated.

关键词： electron-positron annihilation charm production Born cross section radiative correction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Myeloid-derived Suppressor Cells Activate Liver Natural Killer Cells in a Murine Model in Uveal Melanoma

引用

Current Medical Science 2022年第5期42卷 1071-1078页

作者： Yuan-yuan wang Shuang-ying LI San-qian CHEN liang-liang wang Zhi-qiang HAN Department of Obstetrics and Gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Obstetrics and Gynecology Chibi People’s HospitalChibi437300China

Objective Elevated myeloid-derived suppressor cells(MDSCs)in many malignancies are associated with the increased risk for metastases and poor ***,a mouse model of intraocular melanoma was established to explore how MDSCs influence liver *** In this study,murine B16LS melanoma cells were transplanted into the posterior compartment(PC)of the eye of C57BL/6 *** from the liver of naive mice and mice bearing melanoma liver metastasis were isolated using isotonic Percoll centrifugation,examined by flow cytometry for their expression of Gr1,CD11b,F4/80,RAE-1,and Mult-1,and further isolated for MDSCs and natural killer(NK)*** effects of MDSCs on NK cells were tested by coculturing and assessing the ability of NK cells to produce interferon-gamma(IFN-γ)by ELISA and NK cell cytotoxicity by 3H-thymidine incorporation *** impact of IFN-γon liver metastases was examined via selectively depleting IFN-γin *** The results showed that mice with liver metastases had increased levels of CD11b+Gr1+F4/80+as well as CD11b+Gr1+F4/80−*** significantly enhanced the generation of IFN-γtogether with the cytotoxicity of the NK ***,these effects were cell-cell *** IFN-γwas not of a toxic nature to the melanoma cells,it profoundly inhibited B16LS cell *** IFN-γin vivo led to increased liver *** All these findings first revealed that MDSCs accumulated in liver metastasis of intraocular melanoma could activate the NK cells to produce an effective anti-tumor immune ***,the MDSCs’performance in different tumor models would need more investigation to boost current immunotherapy modalities.

关键词： myeloid-derived suppressor cells natural killer cells IFN-γ liver metastases

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quantum key distribution transmitter chip based on hybrid-integration of silica and lithium niobates

引用

Chinese Physics B 2022年第6期31卷 415-421页

作者： Xiao Li liang-liang wang Jia-shun Zhang Wei Chen Yue wang Dan Wu Jun-Ming An State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of Sciences(CAS)Beijing 100083China College of Materials Science and Opto-Electronic Technology University of Chinese Academy of SciencesBeijing 100049China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China

A quantum key distribution transmitter chip based on hybrid-integration of silica planar light-wave circuit(PLC)and lithium niobates(LN)modulator PLC is *** silica part consists of a tunable directional coupler and 400-ps delay line,and the LN part is made up of a Y-branch,with electro-optic modulators on both *** two parts are facet-coupled to form an asymmetric Mach-Zehnder *** successfully encode and decode four BB84 states at 156.25-MHz repetition *** phase-encoding of 0 orπis achieved,with interference fringe visibilities 78.53%and 82.68%for states|+〉and|-〉,*** the aid of an extra off-chip LN intensity modulator,two time-bin states are prepared and the extinction ratios are 18.65 dB and 15.46 dB for states|0〉and|1〉,respectively.

关键词： quantum key distribution hybrid-integration BB84

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Geometrical transition properties of vortex cavitation and associated flow-choking characteristics in poppet valves

引用

Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第6期25卷 455-469页

作者： liang LU Zhongdong liang Yuming LIU Zhipeng wang Shohei RYU School of Mechanical Engineering Tongji UniversityShanghai 201804China Frontiers Science Center for Intelligent Autonomous Systems Tongji UniversityShanghai 201210China Technical Research Laboratory Hitachi Construction Machinery Co.Ltd.Tsuchiura 300-0013Japan

Poppet valves have become increasingly significant in ensuring precise digital flow rate and pressure control in hydraulic systems,necessitating a more profound understanding of the geometrical properties of cavitation in them,as well as associated flow-choking *** a comparative analysis with experimentally observed cavity images,we found that large eddy simulation(LES)turbulence modeling effectively replicates the geometrical properties of cavitation in these *** analysis demonstrated that cavitation is generated from vortices that result from the interaction between the notch contracta flow and the surrounding fluid *** in the internal or external vena contracta conditions result in fixed or discrete cavities,and the length-to-diameter ratio serves as a measure of the transition between internal and external vena contracta flow *** study establishes a threshold length-to-diameter ratio of approximately 2 for the tested poppet *** specifically,in notch structures with a smaller valve opening,longer sealing length,and smaller throttling angle(corresponding to a larger length-to-diameter ratio),the liquid-to-vapor transfer process is more evident than that in the reverse direction.A long-standing vapor cavity becomes fixed inside the notch,leading to a more pronounced flow-choking *** contrast,for structures with a smaller length-to-diameter ratio,the cavitation process for discrete vapor cavities is more complete,ensuring fluid flow continuity and significantly reducing the occurrence of the flow-choking phenomenon.

关键词： Poppet valves Vena contracta Vortex flow Vapor cavity Flow-choking

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Potential role of microRNA-503 in Icariin-mediated prevention of high glucose-induced endoplasmic reticulum stress

引用

World Journal of Diabetes 2023年第8期14卷 1234-1248页

作者： Bao-Lin Su liang-liang wang liang-You Zhang Shu Zhang Qiang Li Gang-Yi Chen Department of Nephrology The First Affiliated Hospital of Guangzhou University of Traditional Chinese MedicineGuangzhou 510405Guangdong ProvinceChina

BACKGROUND Dysregulated microRNA(miRNA)is crucial in the progression of diabetic nephropathy(DN).AIM To investigate the potential molecular mechanism of Icariin(ICA)in regulating endoplasmic reticulum(ER)stress-mediated apoptosis in high glucose(HG)-induced primary rat kidney cells(PRKs),with emphasis on the role of miR-503 and sirtuin 4(SIRT4)in this *** Single intraperitoneal injection of streptozotocin(65 mg/kg)in Sprague-Dawley rats induce DN in the in vivo hyperglycemic ***-treated PRKs were used as an in vitro HG *** immunofluorescence assay identified isolated *** Counting Kit-8 and flow cytometry analyzed the effect of ICA treatment on cell viability and apoptosis,***-time quantitative polymerase chain reaction and western blot analyzed the levels of ER stressrelated *** luciferase analysis of miR-503 binding to downstream SIRT4 was *** ICA treatment alleviated the upregulated miR-503 expression in vivo(DN)and in vitro(HG).Mechanistically,ICA reduced HG-induced miR-503 overexpression,thereby counteracting its function in downregulating SIRT4 *** regulated the miR-503/SIRT4 axis and subsequent ER stress to alleviate HG-induced PRKs *** ICA reduced HG-mediated inhibition of cell viability,promotion of apoptosis,and ER stress in *** effects involved regulation of the miR-503/SIRT4 *** findings indicate the potential of ICA to treat DN,and implicate miR-503 as a viable target for therapeutic interventions in DN.

关键词： Icariin MicroRNA-503 Sirtuin 4 Endoplasmic reticulum stress Diabetic nephropathy Kidney damage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Boundary Element Method for Ordinary State-Based Peridynamics

引用

Computer Modeling in Engineering & Sciences 2024年第6期139卷 2807-2834页

作者： Xue liang Linjuan wang State Key Laboratory for Turbulence and Complex Systems Department of Mechanics and Engineering ScienceCollege of EngineeringPeking UniversityBeijing100871China CAPT-HEDPS and IFSA Collaborative Innovation Center of MoECollege of EngineeringPeking UniversityBeijing100871China School of Astronautics Beihang UniversityBeijing100191China

The peridynamics(PD),as a promising nonlocal continuum mechanics theory,shines in solving discontinuous *** to now,various numerical methods,such as the peridynamic mesh-free particlemethod(PD-MPM),peridynamic finite element method(PD-FEM),and peridynamic boundary element method(PD-BEM),have been ***-BEM,in particular,outperforms other methods by eliminating spurious boundary softening,efficiently handling infinite problems,and ensuring high computational ***,the existing PD-BEM is constructed exclusively for bond-based peridynamics(BBPD)with fixed Poisson’s ratio,limiting its applicability to crack propagation problems and scenarios involving infinite or semi-infinite *** this paper,we address these limitations by introducing the boundary element method(BEM)for ordinary state-based peridynamics(OSPD-BEM).Additionally,we present a crack propagationmodel embeddedwithin the framework ofOSPD-BEM to simulate crack *** validate the effectiveness of OSPD-BEM,we conduct four numerical examples:deformation under uniaxial loading,crack initiation in a double-notched specimen,wedge-splitting test,and threepoint bending *** results demonstrate the accuracy and efficiency of OSPD-BEM,highlighting its capability to successfully eliminate spurious boundary softening phenomena under varying Poisson’s ***,OSPDBEMsignificantly reduces computational time and exhibits greater consistencywith experimental results compared to PD-MPM.

关键词： Ordinary state-based peridynamics boundary element method crack propagation fracture toughness

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：