Potential role of microRNA-503 in Icariin-mediated prevention of high glucose-induced endoplasmic reticulum stress

作     者：Bao-Lin Su Liang-Liang Wang Liang-You Zhang Shu Zhang Qiang Li Gang-Yi Chen 

作者机构：Department of NephrologyThe First Affiliated Hospital of Guangzhou University of Traditional Chinese MedicineGuangzhou 510405Guangdong ProvinceChina 

出 版 物：《World Journal of Diabetes》 (世界糖尿病杂志（英文版）（电子版）)

年 卷 期：2023年第14卷第8期

页      面：1234-1248页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：The First Affiliated Hospital of Guangzhou University of Chinese Medicine Innovation and Strengthening Fund No.2019QN14 

主　　题：Icariin MicroRNA-503 Sirtuin 4 Endoplasmic reticulum stress Diabetic nephropathy Kidney damage 

摘      要：BACKGROUND Dysregulated microRNA(miRNA)is crucial in the progression of diabetic nephropathy(DN).AIM To investigate the potential molecular mechanism of Icariin(ICA)in regulating endoplasmic reticulum(ER)stress-mediated apoptosis in high glucose(HG)-induced primary rat kidney cells(PRKs),with emphasis on the role of miR-503 and sirtuin 4(SIRT4)in this *** Single intraperitoneal injection of streptozotocin(65 mg/kg)in Sprague-Dawley rats induce DN in the in vivo hyperglycemic ***-treated PRKs were used as an in vitro HG *** immunofluorescence assay identified isolated *** Counting Kit-8 and flow cytometry analyzed the effect of ICA treatment on cell viability and apoptosis,***-time quantitative polymerase chain reaction and western blot analyzed the levels of ER stressrelated *** luciferase analysis of miR-503 binding to downstream SIRT4 was *** ICA treatment alleviated the upregulated miR-503 expression in vivo(DN)and in vitro(HG).Mechanistically,ICA reduced HG-induced miR-503 overexpression,thereby counteracting its function in downregulating SIRT4 *** regulated the miR-503/SIRT4 axis and subsequent ER stress to alleviate HG-induced PRKs *** ICA reduced HG-mediated inhibition of cell viability,promotion of apoptosis,and ER stress in *** effects involved regulation of the miR-503/SIRT4 *** findings indicate the potential of ICA to treat DN,and implicate miR-503 as a viable target for therapeutic interventions in DN.