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Formylation of N-arylpyrazole containing active amino group using Vilsmeier-Haack reaction

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Chinese Chemical Letters 2008年第4期19卷 383-386页

作者： yi Luo Ping zhong Xiao hong Zhang Qiu lian Lin Ye Na Chen College of Chemistry and Materials Science Wenzhou UniversityWenzhou 325027China

Two species of N-arylpyrazoles containing active amino group were *** formylations of N-arylpyazoles containing amino in different position of pyrazole rings using Vilsmeier-Haack reaction gave a series of useful pyrazole *** important features of this protocol were cheap materials,easy process,mild reaction conditions and good yield of products.

关键词： Vilsmeier-Haack reaction N-arylpyrazole Formylation Amino Synthesis

Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

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Journal of Integrative Agriculture 2014年第6期13卷 1331-1339页

作者： yang Ya-lan LI Yan lianG Ru-yi ZHOU Rong AO hong MU Yu-lian yang Shu-lin LI Kui TANG zhong-lin Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation Ministry of Agriculture/Institute of Animal ScienceChinese Academy of Agricultural Sciences Department of Animal Genetics Breeding and ReproductionCollege of Animal Science and TechnologyNanjing Agricultural University

MicroRNAs （miRNAs）, evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression proifles of miRNA-127 were carried out using step-loop quantitative real-time PCR （stem-loop RT-PCR）. To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed signiifcant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many signiifcantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs.

关键词： porcine microRNA-127 expression profiles skeletal muscle development target prediction

Diagnostic and prognostic roles of soluble CD22 in patients with Gram-negative bacterial sepsis

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Hepatobiliary & Pancreatic Diseases International 2015年第5期14卷 523-529页

作者： yi-Nan Jiang xun Cai hong-Min Zhou Wei-Dong Jin Min Zhang Yong Zhang Xiao-Xiao Du zhong-Hua Klaus Chen Institute of Organ Transplantation Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Key Laboratory of Ministry of Health and Key Laboratory of Ministry of Education Department of General Surgery Wuhan General Hospital of Guangzhou Military Command Department of Cardiothoracic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

BACKGROUND： Soluble CD22（sCD22） is a fragment of CD22, a B cell-specific membrane protein that negatively regulates B-cell receptor signaling. To date, sCD22 has only been regarded as a tumor marker of B-cell malignancies. Its expression in infectious diseases has not yet been ***： Serum concentrations of sCD22, procalcitonin（PCT） and interleukin-6（IL-6） were measured by enzymelinked immunosorbent assays in patients with intra-abdominal Gram-negative bacterial infection. Receiver operating characteristic curve analysis was performed to evaluate the diagnostic accuracy of these biomarkers in this type of infection. The correlations between biomarkers and the Acute Physiology and Chronic Health Evaluation（APACHE） II scores were also ***： Concentrations of sCD22 were significantly elevated in patients with sepsis and the elevation is correlated with the severity of sepsis. sCD22 was also slightly elevated in patients with non-infected systemic inflammatory response syndrome or local infection. The diagnostic accuracy of sCD22 for sepsis was equivalent to that of PCT or IL-6. In addition, the correlation of sCD22 with APACHE II scores was stronger than that of PCT or ***： Serum sCD22 is a novel inflammatory mediator released during infection. This soluble biomarker plays a potential role in the diagnosis of Gram-negative bacterial sepsis, with a diagnostic accuracy as efficient as that of PCT or IL-6. Furthermore, sCD22 is more valuable to predict the outcomes in patients with sepsis than PCT or IL-6. The present study suggested that sCD22 might be potentially useful in supplementing current criteria for sepsis.

关键词： soluble CD22 procalcitonin interleukin-6 sepsis systemic inflammatory response syndrome

A Novel Surgery Classification for Endoscopic Approaches to Middle Ear Cholesteatoma

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Current Medical Science 2020年第1期40卷 9-17页

作者： Yu SUN En-hao WANG Jin-tao YU Gang zhong Li-xin ZHU-yi WANG Niu xun hong YU Wen ZHOU Zhen XIE Kun ZHANG Guo-run FAN yi zhong hong-jun XIAO Wei-jia KONG Department of Otorhinolaryngology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

This study aimed to develop a novel surgery classification for an endoscopic approach to middle ear *** retrospectively analyzed the surgical approaches and outcomes of patients with middle ear *** ear cholesteatoma surgeries were divided into four types and two special types as follows:type I,attic retraction pocket,which only requires tympanostomy tube placement or retraction pocket resection and cartilage reconstruction;typeⅡ,cholesteatoma which is limited to the attic or in which endoscopy can confirm complete removal of mastoid cholesteatoma lesions,including type Ⅱa,requiring only use of a curette,and type Ⅱ b,requiring use of an electric drill or chisel;type Ⅲ,cholesteatoma not limited to the attic,in which endoscopy cannot confirm complete removal of mastoid cholesteatoma lesions,requiring the combined use of endoscope and microscope to perform endoscopic tympanoplasty and"Canal Wall Up"mastoidectomy;type Ⅳ,extensive involvement of mastoid cavity cholesteatoma lesions and/or cases with a potential risk of complications,removal of which can only be performed under a microscope for"Canal Wall Down"*** addition,there were two special types:"difficult external auditory canal"and congenital cholesteatoma in *** our system,type I and type U middle ear cholesteatoma surgery was completely performed under an endoscope ***,estimating the extent of the lesions,determining the choice of mastoid opening and reestablishing ventilation are the key points for an endoscopic approach to middle ear *** classification of endoscopic middle ear cholesteatoma surgery may benefit the selection of surgical indications.

关键词： otologic surgical procedure endoscopy cholesteatoma middle ear

Parameters optimization design of quenching and partitioning for best combination between strength and ductility using orthogonal experimental design

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Journal of Iron and Steel Research International 2019年第10期26卷 1088-1095页

作者： Ming-qing Liao zhong-hong Lai Aorigele Bao Yong Liu Dan-ni yang Tian-yi Han Jing-chuan Zhu Rong-da Zhao School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001HeilongjiangChina The Center of Analysis Measurement Harbin Institute of TechnologyHarbin 150001HeilongjiangChina National Key Laboratory for Precision Hot Processing of Metals Harbin Institute of TechnologyHarbin 150001HeilongjiangChina School of Materi

Quenching and partitioning(Q&P)which provides a balance between toughness and strength is a promising heat treatment in iron and steel ***,there are three parameters(quenching temperature,partitioning temperature and partitioning time)which affect the properties *** a result,it remains a challenge to get the best parameters in a low-cost way for Q&P ***,the orthogonal experimental design combined with a local optimization was adopted to optimize the quenching and partitioning parameters of 65Si2MnWA *** using this method,the combination between strength and ductility was optimized;meanwhile,the number of experiments was reduced *** treated by quenching at 180℃followed by partitioning at 330℃for 20 min,the steel reached the best combination between strength and *** detail,the product of ultimate tensile strength and reduction in area was 1.36 times(from 64.9 to 88.8 GPa%)that treated by quenching and *** addition,owing to the grain refinement,the strength and ductility increase ***,the reduction in area increased by 27.4%(from 35.8%to 45.6%)coupled with a little improvement in ultimate tensile strength(7.4%).

关键词： Parameter optimization design Quenching and partitioning Orthogonal experimental design Mechanical property 65Si2MnWA steel

Chromosome-scale assembly of the Kandelia obovata genome

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Horticulture Research 2020年第1期7卷 1823-1834页

作者： Min-Jie Hu Wei-hong Sun Wen-Chieh Tsai Shuang Xiang Xing-Kai Lai De-Qiang Chen Xue-Die Liu yi-Fan Wang yi-xun Le Si-Ming Chen Di-yang Zhang Xia Yu Wen-Qi Hu Zhuang Zhou Yan-Qiong Chen Shuang-Quan Zou zhong-Jian Liu Key Laboratory of Humid Sub-tropical Eco-Geographical Processes of the Ministry of Education Fujian Normal UniversityFuzhou 350007China Fujian Colleges and Universities Engineering Research Institute of Conservation and Utilization of Natural Bioresources College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at the College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Tropical Plant Sciences and Microbiology National Cheng Kung UniversityTainan 701China Administration of the Quanzhou Bay Estuary Wetland Nature Reserve Quanzhou 362000China Ocean College Minjiang UniversityFuzhou 350002China Henry Fok College of Biology and Agriculture Shaoguan UniversityShaoguan 512005China

The mangrove Kandelia obovata(Rhizophoraceae)is an important coastal shelterbelt and landscape tree distributed in tropical and subtropical areas across East Asia and Southeast ***,a chromosome-level reference genome of *** based on PacBio,Illumina,and Hi-C data is *** high-quality assembled genome size is 177.99 Mb,with a contig N50 value of 5.74 Mb.A large number of contracted gene families and a small number of expanded gene families,as well as a small number of repeated sequences,may account for the small *** *** found that *** experienced two whole-genome polyploidization events:one whole-genome duplication shared with other Rhizophoreae and one shared with most eudicots(γevent).We confidently annotated 19,138 protein-coding genes in *** and identified the MADS-box gene class and the RPW8 gene class,which might be related to flowering and resistance to powdery mildew in *** and Rhizophora apiculata,*** reference *** genome described here will be very useful for further molecular elucidation of various traits,the breeding of this coastal shelterbelt species,and evolutionary studies with related taxa.

关键词： breeding coastal landscape

响应曲面法在类固醇雌激素HPLC-MS-MS定量分析中的应用

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重庆大学学报（自然科学版） 2011年第5期34卷 105-111页

作者：万巧玲阳春张智廉鹏易中宏重庆大学三峡库区生态环境教育部重点实验室重庆400045 重庆医药工业研究院有限责任公司重庆400061

雌酮(E1)、17β雌二醇(E2)和17α乙炔基雌二醇(EE2)是雌情活力最强的3种类固醇雌激素。通过响应曲面法(RSM)优化了3种雌激素在高效液相色谱串联质谱联用(HPLC-MS-MS)检测方法中的多离子监测反应(MRM)条件,获得E1、E2和EE2的最优碎片电... 详细信息

雌酮(E1)、17β雌二醇(E2)和17α乙炔基雌二醇(EE2)是雌情活力最强的3种类固醇雌激素。通过响应曲面法(RSM)优化了3种雌激素在高效液相色谱串联质谱联用(HPLC-MS-MS)检测方法中的多离子监测反应(MRM)条件,获得E1、E2和EE2的最优碎片电压分别为205.0、196.0和182.0V,最优碰撞能分别为43.0、51.0和54.0V。配合固相萃取(SPE)预处理技术,采用HPLC-MS-MS内标检测方法检测了重庆主城区嘉陵江水样中E1、E2和EE2的浓度。3者在该方法中的定量检测限分别为0.5、0.4和0.1ng/L。

关键词：污水分析液质联用雌酮 17β一雌二醇 17α一乙炔基雌二醇响应曲面法

Epidemiological and clinical characteristics of COVID-19 patients in Hengyang,Hunan Province,China

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World Journal of Clinical Cases 2020年第12期8卷 2554-2565页

作者： Zhe-Feng zhong Jia Huang Xia yang Jin-Ling Peng Xiao-Yan Zhang yang Hu Nian Fu Hai-lian Lin Bo Jiang Ya-ying Tian hong-yi Yao Li-Pu Deng Xiao-Qing Tang Jie-Can Zhou Jian Tang Xia Xie Qiong Liu Jing Liu Cheng-Yun Dou Rong-Juan Dai Bo Yan Xue-Feng yang Department of Infectious Diseases the Affiliated Nanhua HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421002Hunan ProvinceChina Department of Gastroenterology the Affiliated Nanhua HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421002Hunan ProvinceChina Department of General Practice the Affiliated Nanhua HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421002Hunan ProvinceChina Department of Intensive Medicine the Affiliated Nanhua HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421002Hunan ProvinceChina Institute of Clinical Medicine the First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421001Hunan ProvinceChina Department of Infectious Diseases the First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421001Hunan ProvinceChina Department of Cardiology the First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyang 421001Hunan ProvinceChina

BACKGROUND In December 2019,an ongoing outbreak of coronavirus disease 2019(COVID-19)was first identified in Wuhan,*** characteristics of COVID-19 patients treated in local hospitals in Wuhan are not fully representative of patients outside ***,it is highly essential to analyze the epidemiological and clinical characteristics of COVID-19 in areas outside Wuhan or Hubei *** date,a limited number of studies have concentrated on the epidemiological and clinical characteristics of COVID-19 patients with different genders,clinical classification,and with or without basic *** To study the epidemiological and clinical characteristics of COVID-19 patients in Hengyang(China)and provide a reliable reference for the prevention and control of *** From January 16 to March 2,2020,a total of 48 confirmed cases of COVID-19 were reported in Hengyang,and those cases were included in this *** diagnostic criteria,clinical classification,and discharge standard related to COVID-19 were in line with the Diagnosis and Treatment Protocol for Novel Coronavirus Pneumonia(Trial Version 7)released by National Health Commission and National Administration of Traditional Chinese *** presence of SARS-CoV-2 in pharyngeal swab specimens was detected by quantitative reverse transcription polymerase chain *** the data were imported into the excel worksheet and statistically analyzed by using SPSS 25.0 *** A total of 48 cases of COVID-19 were collected,of which 1 was mild,38 were moderate,and 9 were *** was unveiled that there were 31(64.6%)male patients and 17(35.4%)female patients,with a female-to-male ratio of 1.82:*** range of age of patients with COVID-19 was dominantly 30-49 years old[25(52.1%)of 48],followed by those aged over 60 years old[11(22.9%)].Besides,29.2%(14 of 48)of patients had basic diseases,and 57.2%(8 of 14)of patients with basic diseases were aged over 60 years *** occupations of 48 CO

关键词： Novel coronavirus pneumonia COVID-19 SARS-CoV-2 Epidemiology Hengyang Coronavirus disease 2019

Specific convulsions and brain damage in children hospitalized for Omicron BA.5 infection:an observational study using two cohorts

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World Journal of Pediatrics 2024年第10期20卷 1079-1089页

作者： Yuan-Yuan Pei hong-Li Wang Gen-Quan yin yi Xu Jian-Hao Tan Xin-Hua liang Hui-ying Wu xun-Tao yin Chun-Xiao Fang Jun-Zheng Peng Zhi-Yuan Wu yi Sun Run Dang Yu-Feng liang hong-Mei Tang You-yi Li zhong-Xiang Qiao Zhi-Cheng liang Jian-Ping Tang Fan-Sen Zeng Ke-Lu Zheng yi-Ru Zeng Xiao-Jun Cao Hui-Min Xia Jian-Rui Wei Jin-Ling Tang Si-Tang Gong Clinical Data Center Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Department of Gastroenterology Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Department of Internal Medicine Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Department of Infectious Diseases Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Information Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China School of Pediatrics Guangzhou Medical UniversityGuangzhou 510623China Imaging Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Respiratory Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Pediatric Intensive Care Unit Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhou510623China Neonatal Intensive Care Unit Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Rehabilitation Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China General Pediatrics Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou510623China Emergency Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Department of Neurology Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Key Laboratory of Structural Birth Defects Research in Guangdong Province Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Cardiovascular Department Guangzhou Women and Children's Medical CenterGuangzhou Medical UniversityGuangzhou 510623China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShen

Background SARS-CoV-2 continues to mutate over time,and reports on children infected with Omicron BA.5 are *** aimed to analyze the specific symptoms of Omicron-infected children and to improve patient *** We selected 315 consecutively hospitalized children with Omicron BA.5 and 16,744 non-Omicron-infected febrile children visiting the fever clinic at our hospital between December 8 and 30,*** convulsions and body temperatures were compared between the two *** analyzed potential associations between convulsions and vaccination,and additionally evaluated the brain damage among severe Omicron-infected *** Convulsion rates(97.5%vs.4.3%,P<0.001)and frequencies(median:2.0 vs.1.6,P<0.001)significantly differed between Omicron-infected and non-Omicron-infected febrile *** body temperatures of Omicron-infected children were significantly higher during convulsions than when they were not convulsing and those of non-Omicron-infected febrile children during convulsions(median:39.5 vs.38.2 and 38.6℃,both P<0.001).In the three Omicron-subgroups,the temperature during convulsions was proportional to the percentage of patients and significantly differed(P<0.001),while not in the three non-Omicron-subgroups(P=0.244).The convulsion frequency was lower in the 55 vaccinated children compared to the 260 non-vaccinated children(average:1.8 vs.2.1,P<0.001).The vaccination dose and convulsion frequency in Omicron-infected children were significantly correlated(P<0.001).Fifteen of the 112 severe Omicron cases had brain *** Omicron-infected children experience higher body temperatures and frequencies during convulsions than those of non-Omicron-infected febrile *** additionally found evidence of brain damage caused by infection with omicron *** and prompt fever reduction may relieve symptoms.

关键词： Body temperature Brain damage:Children:Convulsion Omicron BA.5.Vaccination