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Morphology, mitochondrial development and adipogenic-related genes expression during adipocytes differentiation in grass carp(Ctenopharyngodon idellus)

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Science Bulletin 2015年第14期60卷 1241-1251页

作者： Pin liu Hong Ji Chao li li-qiao chen Zhen-Yu Du College of Animal Science and Technology Northwest A&F University Station of China Fisheries Law Enforcement School of life Sciences East China Normal University

To investigate the differentiation mechanism of grass carp preadipocytes, a primary adipocytes culture system was established. Confluent preadipocytes were induced to differentiation, and the morphology and gene expression were evaluated at different stages. It was shown that preadipocytes were gradually filled with droplets and the cellular lipid content increased during the differentiation. Ultrastructure observation indicated that the number of mitochondria increased with adipocytes differentiation. Consistently, the mitochondrial protein content was ele- vated in the differentiating adipocytes, qRT-PCR showed that the expression level of lipogenesis-related genes such as peroxisome proliferator activator receptor 7 （PPAR 7）, lipoprotein lipase （LPL）, fatty acid synthase （FAS） and stearoyl-CoA desaturase （SCD） increased during adipocytes differentiation. The mitochondrial relevant gene also elevated when adipocyte differentiation, such as PPAR coactivator-1 （PGC-1 α）, PGC-1β and nuclear respiratory factor （NRF-1）. However, the expression of carnitine palmitoyltransferase 1α （CPT-1 α） gene decreased at the initial stage, but increased at the last stage of cell differ- entiation. These results indicated that the differentiation process of grass carp preadipocytes is similar to that of land animals, but the molecular mechanisms are not exactly the same. The findings revealed in this study provides new information to the study of fish adipocyte differentiation.

关键词： Grass carp. Adipocytes. DifferentiationMitochondria

Double-edged effect of sodium citrate in Nile tilapia(Oreochromis niloticus):Promoting lipid and protein deposition *** hyperglycemia and insulin resistance

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Animal Nutrition 2023年第3期14卷 303-314页

作者： Jun-Xian Wang Fang qiao Mei-ling Zhang li-qiao chen Zhen-Yu Du Yuan Luo Laboratory of Aquaculture Nutrition and Environmental Health(LANEH) School of Life SciencesEast China Normal UniversityShanghaiChina

Citrate is an essential substrate for energy metabolism that plays critical roles in regulating glucose and lipid metabolic ***,the action of citrate in regulating nutrient metabolism in fish remains poorly ***,we investigated the effects of dietary sodium citrate on growth performance and systematic energy metabolism in juvenile Nile tilapia(Oreochromis niloticus).A total of 270Nile tilapia(2.81±0.01 g)were randomly divided into three groups(3 replicates per group,30 fish per replicate)and fed with control diet(35%protein and 6%lipid),2%and 4%sodium citrate diets,respectively,for 8 *** results showed that sodium citrate exhibited no effect on growth performance(P>0.05).The whole-body crude protein,serum triglyceride and hepatic glycogen contents were significantly increased in the 4%sodium citrate group(P0.05).The 4%sodium citrate treatment significantly increased the serum glucose and insulin levels at the end of feeding trial and also in the glucose tolerance test(Pling and inhibiting AMPK signaling(P<0.05).Furthermore,the 4%sodium citrate significantly suppressed serum aspartate aminotransferase and alanine aminotransferase activities,along with the lowered expression of pro-inflammatory genes,such as nfκb,tnfa and il8(P<0.05).Although the 4%sodium citrate significantly increased phosphor-nuclear factor-k B p65 prote

关键词： Sodium citrate lipid and protein deposition Insulin resistance Nile tilapia

High cholesterol intake remodels cholesterol turnover and energy homeostasis in Nile tilapia(Oreochromis niloticus)

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Marine life Science & Technology 2023年第1期5卷 56-74页

作者： Rui-Xin li ling-Yun chen Samwel M.limbu Yu-cheng Qian Wen-Hao Zhou li-qiao chen Yuan Luo Fang qiao Mei-ling Zhang Zhen-Yu Du LANEH School of Life SciencesEast China Normal UniversityShanghai200241China Department of Aquaculture Technology School of Aquatic Sciences and Fisheries TechnologyUniversity of Dar es SalaamP.O.Box 60091Dar es SalaamTanzania

The roles of dietary cholesterol in fish physiology are currently *** issue reflects the limited studies on the metabolic consequences of cholesterol intake in *** present study investigated the metabolic responses to high cholesterol intake in Nile tilapia(Oreochromis niloticus),which were fed with four cholesterol-contained diets(0.8,1.6,2.4 and 3.2%)and a control diet for eight *** fish-fed cholesterol diets showed increased body weight,but accumulated cholesterol(the peak level was in the 1.6%cholesterol group).Then,we selected 1.6%cholesterol and control diets for further *** high cholesterol diet impaired liver function and reduced mitochondria number in ***,high cholesterol intake triggered protective adaptation via(1)inhibiting endogenous cholesterol synthesis,(2)elevating the expression of genes related to cholesterol esterification and efflux,and(3)promoting chenodeoxycholic acid synthesis and ***,high cholesterol intake reshaped the fish gut microbiome by increasing the abundance of Lactobacillus *** Mycobacterium spp.,both of which are involved in cholesterol and/or bile acids ***,high cholesterol intake inhibited lipid catabolic activities through mitochondrialβ-oxidation,and lysosome-mediated lipophagy,and depressed insulin signaling *** catabolism was elevated as a compulsory response to maintain energy ***,although high cholesterol intake promoted growth,it led to metabolic disorders in *** the first time,this study provides evidence for the systemic metabolic response to high cholesterol intake in *** knowledge contributes to an understanding of the metabolic syndromes caused by high cholesterol intake or deposition in fish.

关键词： Cholesterol metabolism Metabolic response Energy metabolism Gut microbiome

Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates

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动物学研究(英文) 2023年第5期44卷 954-966页

作者： Qiang Ma Yuan Luo Jia Zhong Samwel Mchele limbu ling-Yu li li-qiao chen Fang qiao Mei-ling Zhang Qiang lin Zhen-Yu Du LANEH School of Life SciencesEast China Normal UniversityShanghai 200241China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdaoShandong 266071China CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouGuangdong 510301China School of Aquatic Sciences and Fisheries Technology University of Dar es SalaamDar es Salaam 60091Tanzania

Hypoxia is a common environmental stress factor in aquatic organisms,which varies among fish ***,the mechanisms underlying the ability of fish species to tolerate hypoxia are not well ***,we showed that hypoxia response in different fish species was affected by lipid catabolism and preference for lipid or carbohydrate energy *** of biochemical lipid catabolism through peroxisome proliferator-activated receptor alpha(Pparα)or increasing mitochondrial fat oxidation in tilapia decreased tolerance to acute hypoxia by increasing oxygen consumption and oxidative damage and reducing carbohydrate catabolism as an energy ***,lipid catabolism inhibition by suppressing entry of lipids into mitochondria in tilapia or individually knocking out three key genes of lipid catabolism in zebrafish increased tolerance to acute hypoxia by decreasing oxygen consumption and oxidative damage and promoting carbohydrate ***,anaerobic glycolysis suppression eliminated lipid catabolism inhibition-promoted hypoxia tolerance in adipose triglyceride lipase(atgl)mutant *** 14 fish species with different trophic levels and taxonomic status,the fish preferentially using lipids for energy were more intolerant to acute hypoxia than those preferentially using *** study shows that hypoxia tolerance in fish depends on catabolic preference for lipids or carbohydrates,which can be modified by regulating lipid catabolism.

关键词： Hypoxia tolerance Catabolic preference lipid Carbohydrate Oxidative damage

温湿度处理对烟用BOPP膜上机适应性的影响研究

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印刷与数字媒体技术研究 2024年第4期 253-261页

作者：刘文婷杨凯诺李文璟褚玮李陈巧王波吕彩虹刘兴海湖北中烟工业有限责任公司技术中心武汉430040 武汉大学图像传播与印刷包装研究中心武汉430061

作为包装膜,双向拉伸聚丙烯(BOPP)已广泛应用于卷烟包装领域中。本研究通过设置标准环境和4种非标准环境,对不同型号的烟用BOPP膜进行了详细的处理研究,明确了环境温湿度对BOPP包装膜包括摩擦性能、热封性能、热收缩性能和力学性能4种... 详细信息

作为包装膜,双向拉伸聚丙烯(BOPP)已广泛应用于卷烟包装领域中。本研究通过设置标准环境和4种非标准环境,对不同型号的烟用BOPP膜进行了详细的处理研究,明确了环境温湿度对BOPP包装膜包括摩擦性能、热封性能、热收缩性能和力学性能4种上机适应性的影响趋势。通过理论计算和分子动力学模拟的方法,验证了烟用BOPP膜在异常温湿度下性能的劣化机制。结果表明,烟用BOPP膜上机适应性均受环境温湿度影响较大。因此,在储存和运输环节应尽量避免异常温湿度,并在性能检测和上机卷接包前进行标准环境的稳定处理。本研究对于企业提高效率和质量控制有重要参考意义。

关键词：双向拉伸聚丙烯(BOPP) 上机适应性温湿度处理分子动力学模拟

Can Industrial Structure Upgrading Restrain Industrial Land Expansion?Evidence from China

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Chinese Geographical Science 2024年第3期34卷 504-518页

作者： chen Wei li qiao ZHANG Sun ZHOU Xue College of Economics and Management Northwest A&F UniversityYangling Shaanxi 712100China Institute of Geographic Sciences&Natural Resources Research Chinese Academy of SciencesBeijing 100101China

China has made great achievements in industrial development and is transforming into a powerful manufacturing ***,the industrial land scale is also ***,whether industrial structure upgrading achieves the purpose of restraining industrial land expansion remains *** calculating the industrial land structure index(ILSI)and industrial land expansion scale(ILES),this study analyzed their temporal and spatial distribution characteristics at both regional and city levels from 2007to 2020 in *** show that industrial land expansion presents a different trend in the four regions,the ILES in the eastern region is the largest,and the speed of industrial land expansion has declined since 2013,but it has gradually increased since *** ILSI of the eastern and central regions is higher than that of the western and northeastern ***,a spatial Durbin model(SDM)has been established to estimate the spatial effect of industrial structure upgrading on industrial land expansion from 2007 ***,industrial structure upgrading has not slowed industrial land *** eastern and western regions require a greater amount of industrial land while upgrading the industrial *** improvement of the infrastructure level and international trade level has promoted industrial land expansion.

关键词： industrial development industrial structure upgrading industrial land expansion regional differences China

Superatom-assembled boranes,carboranes,and low-dimensional boron nanomaterials based on aromatic icosahedral B_(12)and C_(2)B_(10)

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Nano Research 2024年第7期17卷 6734-6740页

作者： qiao-qiao Yan Yan-Fang Wei Qiang chen Yue-Wen Mu Si-Dian li Institute of Molecular Science Shanxi UniversityTaiyuan 030006China

Using the experimentally known aromatic icosahedral superatoms I_(h)B_(12)H_(12)2−and D_(5d)1,12-C_(2)B_(10)H_(12)as building blocks and based on extensive density functional theory calculations,we predict herein a series of core–shell superpolyhedral boranes and carboranes in a bottom-up approach,including the high-symmetry Th B_(12)@B_(152)H_(72)2−(2),C2h C_(2)B_(10)@B_(152)H_(72)(3),D_(3d)B_(12)@B_(144)H_(66)(4),I_(h)B_(12)@C_(24)B_(12)0H_(72)2−(6),and D_(5d)C_(2)B_(10)@C_(24)B_(12)0H_(72)(7).More interestingly,the superatom-assembled linear D2h B_(36)H_(32)^(2−)(8),close-packed planar D_(3d)B_(84)H_(60)^(2−)(10),and nearly close-packed core−shell D_(3d)B_(12)@B144H_(6)6(4)can be extended periodically to form the one-dimensional(1D)α-rhombohedral borane nanowire B_(12)H_(10)(Pmmm)(9),two-dimensional(2D)α-rhombohedral monolayer borophane B_(12)H_(6)(P m1)(11),and the experimentally known three-dimensional(3D)α-rhombohedral boron(R m)(12)which can be viewed as an assembly of the monolayer B_(12)H_(6)(11)staggered in vertical direction,setting up a bottom-up strategy to form low-dimensional boron-based nanomaterials from their borane“seeds”via partial or complete *** bonding analyses indicate that the high stability of these nanostructures originates from the spherical aromaticity of their icosahedral B_(12)or C_(2)B_(10)structural units which possess the universal skeleton electronic configuration of 1S21P61D101F8 following the Wade’s n+1 *** infrared(IR)and Raman spectra of the most-concerned neutral B_(12)@B144H_(6)6(4)and C_(2)B_(10)@C_(24)B_(12)0H_(72)(7)are computationally simulated to facilitate their experimental characterizations.

关键词： superatom-assembling bottom-up approach density functional theory superpolyhedral boranes superpolyhedral carboranes borophanes

Platelet-rich plasma gel in combination with Schwann cells for repair of sciatic nerve injury

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Neural Regeneration Research 2012年第29期7卷 2286-2292页

作者： Fagang Ye Haiyan li Guangxi qiao Feng chen Hao Tao Aiyu Ji Yanling Hu Department of Trauma Surgery Affiliated Hospital of Medical College Qingdao University Qingdao 266000 Shandong Province China

Bone marrow mesenchymal stem cells were isolated from New Zealand white rabbits, culture-expanded and differentiated into Schwann cell-like cells. Autologous platelet-dch plasma and Schwann cell-like cells were mixed in suspension at a density of 1 x 106 cells/mL, prior to introduction into a poly （lactic-co-glycolic acid） conduit. Fabricated tissue-engineered nerves were implanted into rabbits to bridge 10 mm sciatic nerve defects （platelet-rich plasma group）. Controls were established using fibrin as the seeding matrix for Schwann cell-like cells at identical density to construct tissue-engineered nerves （fibrin group）. Twelve weeks after implantation, toluidine blue staining and scanning electron microscopy were used to demonstrate an increase in the number of regenerating nerve fibers and thickness of the myelin sheath in the platelet-rich plasma group compared with the fibrin group. Fluoro-gold retrograde labeling revealed that the number of Fluoro-gold-positive neurons in the dorsal root ganglion and the spinal cord anterior horn was greater in the platelet-rich plasma group than in the fibrin group. Electrophysiological examination confirmed that compound muscle action potential and nerve conduction velocity were superior in the platelet-rich plasma group compared with the fibrin group. These results indicate that autologous platelet-rich plasma gel can effectively serve as a seeding matrix for Schwann cell-like cells to construct tissue-engineered nerves to promote perJpheral nerve regeneration.

关键词： platelet-rich plasma extracellular matrix Schwann cells fibrin sciatic nerve peripheral nerve injury nerve tissue engineering neural regeneration

Dynamic response of mountain tunnel,bridge,and embankment along the Sichuan-Tibet transportation corridor to active fault based on model tests

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Journal of Mountain Science 2024年第1期21卷 182-199页

作者： HUANG Beixiu qiao Sijia chen Xulei li lihui QI Shengwen Key Laboratory of Shale Gas and Geoengineering Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

The Sichuan-Tibet transportation corridor is prone to numerous active faults and frequent strong *** extensive studies have individually explored the effect of active faults and strong earthquakes on different engineering structures,their combined effect remains *** research employed multiple physical model tests to investigate the dynamic response of various engineering structures,including tunnels,bridges,and embankments,under the simultaneous influence of cumulative earthquakes and stick-slip misalignment of an active *** prototype selected for this study was the Kanding No.2 tunnel,which crosses the Yunongxi fault zone within the Sichuan-Tibet transportation *** results demonstrated that the tunnel,bridge,and embankment exhibited amplification in response to the input seismic wave,with the amplification effect gradually decreasing as the input peak ground acceleration(PGA)*** PGAs of different engineering structures were weakened by the fault rupture ***,the misalignment of the active fault may decrease the overall stiffness of the engineering structure,leading to more severe damage,with a small contribution from seismic ***,the seismic vibration effect might be enlarged with the height of the engineering structure,and the tunnel is supposed to have a smaller PGA and lower dynamic earth pressure compared to bridges and embankments in strong earthquake zones crossing active *** findings contribute valuable insights for evaluating the dynamic response of various engineering structures crossing an active fault and provide an experimental reference for secure engineering design in the challenging conditions of the Sichuan-Tibet transportation corridor.

关键词： Dynamic response Engineering structure Sichuan-Tibet transportation corridor Active fault Earthquake Model test