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Evaluation of Sensitivities and Specificities of SARS-CoV Detection by Real-time Quantitative Reverse Transcription-PCR Assays

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Virologica Sinica 2009年第3期24卷 187-193页

作者： li-li xu zhi-hong HU Hua-lin WANG Xiao HAN Fei DENG State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

The etiological agent of severe acute respiratory syndrome(SARS) was identified as a new coronavirus,termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materials is crucial for SARS control. In this study,we quantified SARS-CoV mRNAs in both infected cell culture lysate and in supernatant by using Real-time quantitative revere transcription-PCR based on EvaGreenTM dye and Taqman-MGB probes. For extensive evaluation of sensitivities and specificities,13 pairs of primers and 4 probes were designed based on different genes of SARS-CoV. Glyceraldehydes-3-phosphate dehydrogenase(GAPDH) was selected as the internal control gene. Results showed that S-gene-specific PCR was the most sensitive for detection,but because of its sequence variability in the different viral strains,primers and a probe based on the N gene were suitable substitutions. Meanwhile,we found the mRNA concentrations in cell culture lysates were much higher than in cell supernatant and facilited more sensitive detection of the SARS-CoV.

关键词： SARS-CoV Sensitivities Specificities Evaluation

Damage of multi-layer spaced metallic target plates impacted by radial layered PELE

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Defence Technology（防务技术） 2020年第1期16卷 201-207页

作者： Chun Cheng Zhong-hua Du Xi Chen li-zhi xu Cheng-xin Du Ji-long Han Xiao-dong Wang School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China

Three different kinds of PELE(the penetrator with lateral efficiency) were launched by ballistic artillery to impact the multi-layer spaced metal target *** lmpact velocities of the projectiles were measured by the velocity measuring *** damage degree and process of each laye r of target plate impacted by the three kinds of projectiles were *** experimental results show that all the three kinds of projectiles have the effect of expanding holes on the multi-layer spaced metal target *** the normal structure PELE(without layered) with tungsten alloy jacket and the radial layered PELE with tungsten alloy jacket,the diameters of holes on the seco nd layer of plates are 3.36 times and 3.76 times of the diameter of the projectile,re *** radial layered PELE with W/Zr-based amorphous composite jacket,due to the large number of tungsten wires dispersed after the impact,the diameter of the holes on the four-layer spaced plates can reach 2.4 times,3.04 times,5.36 times and 2.68 times of the diameter of the ***,the normal structure PELE with tungsten alloy jacket and the radial layered PELE whit tungsten alloy jacket formed a large number of fragments impact marks on the third target *** the number of fragments penetrating the third target plate is not as large as that of the normal structure PELE,the area of dispersion of fragments impact craters on the third target plate is larger by the radial layered *** radial layered PELE with W/Zr-based amorphous composite jacket released a lot of heat energy due to the impact of the matrix material,and formed a large area of ablation marks on the last three target plates.

关键词： PELE(the penetrator with lateral efficiency) Multi-layer spaced metal target plates Impact Damage

Opening behavior of PELE perforation on reinforced concrete target

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Defence Technology（防务技术） 2021年第3期17卷 898-911页

作者： li-zhi xu Jiang-bo Wang Xiao-dong Wang Wen-ke Ren Tian-xing Zhang Zhong-hua Du Guang-fa Gao School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China

Impact velocity(v0),target strength(fc)and target thickness(hc)are important factors affecting opening damage■of PELE penetration into RC *** this paper,based on the three influence factors of v0,fc and hc,experimental and numerical simulation studies on PELE penetration into RC target were carried *** study results show that:(1)Since interaction force(or penetration resistance)between projectile and target is positively correlated with v0 and fc,with the increase of v0 and fc,deformation mode of jacket is changed from small bending deformation to large bending deformation and then to curling ***,the variation of jacket deformation mode causes opening diameter of RC target to increase first and then to *** is found that the two factors approximately satisfy a quadratic function relationship,respectively.(2)For PELE projectile penetrating RC targets with thickness of 80-400 mm,the opening diameter of six sets of RC targets grows from 240 to 500 mm,and hc with■approximately satisfy a linear relationship.(3)Based on the above study results,the relationship between two dimensionless parameters■and dimensionless opening diameter■was *** with the results of previous research,a dimensionless opening diameter model■was *** tests verified,the test results are all within±10%error of the theoretical model,which verifies the accuracy of the model.

关键词： PELE Penetration Reinforced concrete Dimensional analysis

Effect of projectile parameters on opening behavior of PELE penetrating RC target

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Defence Technology（防务技术） 2021年第3期17卷 859-873页

作者： li-zhi xu Cheng-xin Du Chun Cheng Xiao-dong Wang Jiang-bo Wang Zhong-hua Du Guang-fa Gao School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China

When a penetrator with enhanced lateral effect(PELE) impacts on a reinforced concrete(RC) target,the target is damaged with a large *** understanding of how PELE projectile parameters affect the opening dimension,is essential for effective design of the PELE *** this study,under the condition that the impact velocity and target parameters(strength and thickness) were fixed values,the important influence factors of the PELE(jacket wall thickness B,jacket material strength Y1,filling material strength Y2 and angle of monolithic jacket θ) were determined by a dimensional *** and simulations of the PELE penetrating the RC target were conducted to analyze the influence of these factors on opening diameter(D,an equivalent diameter under relative kinetic energy).Based on the test and simulation results,it is found that the influence of these factors B,Y1 and θ on the deformation mode of the jacket shows a similar trend:as values of the three factors decrease,the jacket deforms from small bending deformation to large one,and then to curling *** causes the opening diameter to first increase with the decrease of these three factors,and then *** is well known that the bending resistance of the jacket is related to these factors B,Y1 and θ.Therefore,a plastic limit bending moment(M0) of the jacket was quoted to characterize the influence of these factors on the bending deformation of the jacket and the opening diameter of the *** influence factor Y2 causes D to first increase with the increase of Y2,and then decreases.A formula was developed to predict the opening diameter,whose influence parameters were considered in a dimensionless *** has been shown that the dimensionless opening diameter D/d1 is dependent on two dimensionless parameters■ and■,where d1 and fc are the outer diameter of the projectile and the compressive strength of the target,respectively.

关键词： Penetrator with enhanced lateral effect Opening behaviour Reinforced concrete target Dimensional analysis

A New Compound with C_2-Symmetry 3R,3'R-Dihydroxy-6,6',6,6'-tetramethyl-2R,2'R,-bis-4H-pyran

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Chinese Chemical Letters 1997年第8期8卷 653-654页

作者： li Bing Yu li xu and zhi Qin WANG(Shanghai Institute of Organic Chemistry. Chinese Acadmey of Sciences 354 Fenglin Road. Shanghai 200032) Shanghai Inst. of Organic Chemistry Chinese Acadmey of Sciences Shanghai 200032 354 Fenglin Road China

The title compound 1 was synthesized from 1.2:5,6-dianhydro-3,4-dianhydro-3,4-isopropylideneD-mannitol 2 through four steps in a total yield of 53%. The absolute configuration of this compound was confirmed by X-ray d... 详细信息

关键词： tetramethyl-2R,2’R Rev Cl bis-4H-pyran A New Compound with C2-Symmetry 3R,3’R-Dihydroxy-6,6

Inhibitory Effect of TCCE on CCl4-induced Overexpression of IL-6 in Acute liver Injury

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 767-772页

作者： Jing GAO, Huan DOU, Xin-Hui TANG, li-zhi xu, Yi-Mei FAN, and Xiao-Ning ZHAOSchool of Medicine, School of life Sciences, Nanjing University, Nanjing 210093, China School of Medicine School of Life Sciences Nanjing UniversityNanjing 210093 China

Terminalia catappa L. leaves have been shown to protect against acute liver injury produced by some hepatotoxicants, but the active components and mechanisms are not clear. This study was designed to characterize the protective effects of the chloroform fraction of the ethanol extract of T. catappa leaves (TCCE) against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice, and analyze the changes in expression level of interleukin-6 (IL-6) in the process. It was found that TCCE pretreatment (10 or 30 mg/kg, ig) protected mice from CCl4 toxicity, as evidenced by the reversed alterations in serum alanine aminotrans-ferase (sALT) and serum aspartate aminotransferase (sAST) activities. Additionally liver tissues were subjected to RT-PCR, Western blot and immunohistochemistry to analyze changes in IL-6 expression. It was found that TCCE markedly suppressed the CCl4-induced over-transcription of IL-6 gene. Consistent with the result, the expression of IL-6 protein was also blocked by TCCE in CCl4-stimula

关键词： acute liver injury TCCE CCl4 IL-6 expression

Clinical application of repetitive transcranial magnetic stimulation for post-traumatic stress disorder:A literature review

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World Journal of Clinical Cases 2021年第29期9卷 8658-8665页

作者： Peng Cheng Ying Zhou li-zhi xu Ya-Fei Chen Ruo-lin Hu Yi-ling Zou Ze-xuan li li Zhang Qi Shun xun Yu ling-Jiang li Wei-Hui li National Clinical Research Center for Mental Disorders Department of PsychiatryThe Second Xiangya Hospital of Central South UniversityChangsha 410011Hunan ProvinceChina Xiangya Medical School The Second Xiangya HospitalCentral South UniversityChangsha 410011Hunan ProvinceChina Department of Psychiatry The Second Xiangya Hospital of Central South UniversityChangsha 410011Hunan ProvinceChina Research Center for Brain Science and Human-like Intelligence Xi’an Jiaotong UniversityXi’an 710049Shannxi ProvinceChina Product Department Solide Brain Medical TechnologyLtd.Xi’an 710043Shannxi ProvinceChina

The efficacy of traditional treatment for post-traumatic stress disorder(PTSD)is still *** transcranial magnetic stimulation(rTMS)has been widely used in the treatment of various types of mental disorders,including *** rTMS has been demonstrated to be effective in many cases,there are still arguments regarding its mechanism and *** review aims to summarize the origin,development,principle,and future direction of rTMS and introduce this neuro-stimulation therapy to relevant clinicians.

关键词： Repetitive transcranial magnetic stimulation Post-traumatic stress disorder Neuro-stimulation Physical therapy

Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients

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World Journal of Gastroenterology 2008年第19期14卷 3074-3080页

作者： Yu-Cai Wang Zheng-HongYu Chang liu li-zhi xu Wen Yu Jia Lu Ren-Min Zhu Guo-li li Xin-Yi Xia Xiao-Wei Wei Hong-Zan Ji Heng Lu Yong Gao Wei-Min Gao Long-Bang Chen Department of Medical Oncology Jinling Hospital Nanjing 210002 Jiangsu Province China Medical School of Nanjing University Nanjing 210093 Jiangsu Province China Department of Experimental Radiation Oncology The University of Texas M.D. Anderson Cancer Center The University of Texas Graduate School of Biomedical Sciences at Houston Houston TX 77030 United States Department of Molecular Pathology The University of Texas M. D. Anderson Cancer Center Houston TX 77030 United States Department of Gastroenterology Jinling Hospital Nanjing 210002 Jiangsu Province China Institute of General Surgery Jinling Hospital Nanjing 210002 Jiangsu Province China Institute of Laboratory Medicine Jinling Hospital Nanjing 210002 Jiangsu Province China Department of Environmental Toxicology The Institute of Environmental and Human Health Texas Tech University Lubbock TX 79409 United States

AIM：To evaluate the diagnostic role of serum RASSF1A promoter hypermethylation in gastric and colorectal adenocarcinoma. METHODS：Methylation-specific polymerase chain reaction （MSPCR） was used to examine the promoter methylation status of the serum RASSF1A gene in 47 gastric adenocarcinoma patients, 45 colorectal adenocarcinoma patients, 60 patients with benign gastrointestinal disease （30 with benign gastric disease and 30 with benign colorectal disease）, and 30 healthy donor controls. Apaired study of RASSF1A promoter methylation status in primary tumor, adjacent normal tissue, and postopertive serum were conducted in 25 gastric and colorectal adenocarcinoma patients who later were underwent surgical therapy. RESULTS：The frequencies of detection of serum RASSF1A promoter hypermethylation in gastric （34.0%） and colorectal （28.9%） adenocarcinoma patients were significantly higher than those in patients with benign gastric （3.3%） or colorectal （6.7%） disease or in healthy donors （0%）（P 〈 0.01）. The methylation status of RASSF1A promoter in serum samples was consistent with that in paired primary tumors, and the MSPCR results for RASSF1A promoter methylation status in paired preoperative samples were consistent with those in postoperative serum samples. The serum RASSF1A promoter hypermethylation did not correlate with patient sex, age, tumor differentiation grade, surgical therapy, or serum carcinoembryonic antigen level. Although the serum RASSF1A promoter hypermethylation frequency tended to be higher in patients with distant metastases, there was no correlation between methylation status and metastasis. CONCLUSION：Aberrant CpG island methylation within the promoter region of RASSF1A is a promising biomarker for gastric and colorectal cancer.

关键词： Gastric cancer Colorectal cancer Genemethylation RASSFIA

Association of alcohol consumption with colorectal cancer risk: a dose-response meta-analysis

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Food Therapy and Health Care 2020年第2期2卷 40-53页

作者： Yan li zhi-li xu Yi-Yi Cui Yong Guo Departmet of Integrative Oncology The First Affiliated Hospital of Zhejiang Chinese Medicine University54 Youdian RoadHangzhou 310006China Department of First Clinical Medicine Zhejiang Chinese Medicine University548Bingwen RoadHangzhou 310053China Departmet of Integrative Oncology The Third Affiliated Hospital of Zhejiang Chinese Medicine University219Moganshan RoadHangzhou 310013China

Background:Currently,there are many meta-analyses on the correlation between alcohol conception and the risk of colorectal cancer,but the findings are *** conducted a dose-response meta-analysis to investigate the association between alcohol intake and the risk of colorectal ***:A literature search was performed on PubMed and EMBASE databases to identify relevant articles published before December 09,*** relative risks(RRs)and 95%confidence intervals(CIs)were calculated using a fixed or random-effects model for the dose-response *** Cochran Q and I2 statistics were used to assess statistical heterogeneity among ***:Five cohort and 19 case-control studies were included in the *** increased intake of 15-39.9g of daily alcohol was related to a 21%growth risk of CRC(RR=1.21,95%CI:1.05-1.38).Our dose-response analysis indicated that for drinkers of light,moderate and heavy level of alcohol drinking,the estimated RRs of CRC were 0.94(95%CI:0.69-1.27),1.08(95%CI:0.93-1.26)and 1.90(95%CI:1.29-2.97)respectively and for drinkers of moderate beer,heavy beer,moderate wine and heavy wine intake,the estimated RRs of CRC were 0.98(95%CI:0.72-1.34),1.48(95%CI:1.05-2.07),0.86(95%CI:0.56-1.34)and 1.04(95%CI:0.83-1.29)*** risks with no significant association were consistent in the subgroup analyses of general alcohol consumption status and ***:Heavy level of alcohol consumption,especially heavy beer intake seems to be associated with an increased the risk of CRC.

关键词： Alcohol consumption Colorectal cancer risk Dose-response Meta-analysis