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study of the effect of atorvastatin on the interaction between ICAM-1 and CD11b by live-cell single-molecule force spectroscopy

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science china Chemistry 2010年第4期53卷 752-758页

作者： li yi1, shi xiaoli2, liu huiliang1, {3. shaoQiong3. ZHANG XueJie2 & FANG XiaoHong2 1Department of Cardiology,{3., beijing 10003., china 2beijing National Laboratory for Molecular sciences Institute of Chemistry, chinese Academy of sciences, beijing 100190, china 3.tate Key Laboratory of Pathogen and Biosecurity beijing Institute of Microbiology and Epidemiology, beijing 100071, china 1. Department of Cardiology the General Hospital of Chinese People’s Armed Police Forces Beijing 100039 China2. Beijing National Laboratory for Molecular Sciences Chinese Academy of Sciences Beijing 100190 China4. Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China3. State Key Laboratory of Pathogen and Biosecurity Beijing 100071 China5. Beijing Institute of Microbiology and Epidemiology Beijing 100071 China

The interaction between the cell adhesion molecule CD11b and its ligand ICAM-1 plays an important role in inflammatory responses in the disease of atherosclerosis. Atorvastatin is a commonly prescribed statin drug which has been considered as one of the most potent therapeutic agents for atherosclerosis due to its lipid-lowering effect. Recently, there is a growing body of evidence that atorvastatin has anti-inflammatory effect. We have applied the advanced method of live-cell single-molecule force spectroscopy to investigate the effect of atorvastatin on adhesion force between ICAM-1 and CD11b. Our result showed that single-molecule binding force of ICAM-1 and CD11b detected by AFM in the living cells was about 40 pN, and atorvastatin did not affect this force by blocking ICAM-1 or CD11b. This was different from the ICAM-1 monoclonal antibody, which could directly reduce the binding force of ICAM-1 and CD11b. Flow cytometry results revealed that atorvastatin pretreatment decreased the ICAM-1 expression in TNF-α activated HUVECs, which may contribute to its anti-inflammatory effect. The study provides a new approach to study anti-inflammatory mechanism for clinic drugs.

关键词： atherosclerosis atorvastatin atomic force microscopy single-molecule force spectroscopy protein-protein interaction

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In vitro release of 1,3.bis(2-chloroethyl)-1-nitrosourea sustained-release microspheres and the distribution in rat brain tissues

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Neural Regeneration Research 2006年第9期1卷 793-796页

作者： Xia li1, liping Guo2, Qin li3.1Central Pharmacy, {3. hospital of chinese {3.’s armed police forces, beijing 10003., china 2Department of Pharmaceutics, Zhengzhou Central hospital, Zhengzhou 450007, Henan Province, china 3.eda International Cardiovascular hospital, Tianjin 3.0457, china Central Pharmacy General Hospital of Chinese People's Armed Police Forces Beijing 100039 China Department of Pharmaceutics Zhengzhou Central Hospital Zhengzhou 450007 Henan Province China Teda International Cardiovascular hospital Tianjin 300457 China

BACKGROUND: The implantation of released chemotherapeutic drugs, which takes biodegradable polymer as vector, into the tumor site can get high concentration and release the drug for a long time, it can directly act on the tumor cells, and reduce the general toxicity. OBJECTIVE: To explore the in vitro and in vivo course of 1,3.bis(2-chloroethyl)-1-nitrosourea (BCNU) sustained-release from BCNU-loaded polylactide (PLA) microspheres (Ms) and location in rat brain tissue. DEsIGN: A repetitive measurement. sETTING:Central Pharmacy, general hospital of {3. people’s armed police forces. MATERIALs: Thirty male sD rats were used. PLA (Mr5000, batch number: KsL83.7) was produced by Wako Pure Chemical Inc.,Ltd. (Japan); BCNU (batch number: 021121) by Tianjin Jinyao Amino Acid Co., Ltd.; BCNU-PLA-Ms was prepared by the method of solvent evaporation and pressed into tablets (10 mg/tablet). High-performance liquid chromatography (HPLC) Agilent 1100 (UsA); Ls9800 liquid-scintillation radiometric apparatus (Beckman). Chromatographic conditions: Elite Hypersil ODs2 C18 chromatographic column (5 μm, 4.6 mm×150 mm); Mobile phase: methanol: water (50:50), flow rate was 1.0 mL per minute, wave length of ultraviolet detection was 23. nm, and the inlet amount of samples was 10 μL. METHODs: The experiments were carried out in the experimental animal center of the general hospital of {3. armed police from May 2004 to July 2005. ① In vitro BCNU-PLA-Ms release test: BCNU-PLA-Ms was prepared by the method of solvent evaporation, then placed in 0.1 mol/L phosphate buffered solution (PBs, pH 7.4, 3. ℃), part of Ms were taken out at 1, 2, 3. 7, 10 and 15 days respectively, and the rest amount of BCNU in Ms was determined by HPLC, then the curve of BCNU-PLA-Ms release was drawn. ②In vivo BCNU-PLA-Ms release and distribution test: The rats were anesthetized, then BCNU-PLA-Ms were implanted to the site 1 mm inferior to the cortex of frontal lobe. Five rats were killed postoperatively at 4 h

关键词： BCNU Ms PLA nitrosourea sustained-release microspheres and the distribution in rat brain tissues chloroethyl

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AFM OBsERVATION OF NORMAL HUMAN ERYTHROCYTEs PROCEssED BY sEVERAL sOLUTIONs

AFM OBSERVATION OF NORMAL HUMAN ERYTHROCYTES PROCESSED BY SE...

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Asia Conference on Nanoscience and Nanotechnology Abstracts Book

作者： YA-MIN MA XIAO-LONG JI yiN TONG MING-shi sHEN Institute of Geratology general {3. of PLA beijing 100853.{3. Institute ofnanomedicine the general {3. of chinese people’s armed police forces beijing 10003. {3.

＜正＞ The application of atomic force microscopy （AFM） in clinical diagnosis is far from being developed. suitable solutions for the observing of ervthrocyte by AFM are necessary. In this study, following solutions, 1% formaldehyde, citrate buffer, 5%dextrose and TAE, were found to keep human erythrocytes in normal shape with few particles. Processed by these solutions, fine structures of human erythrocyte’s membrane were obtained. The results will offer meaningful data for clinical diagnosis of blood by AFM.

关键词： Atomic Force Microscopy solution erythrocyte fine structures

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A novel flexible nerve guidance conduit promotes nerve regeneration while providing excellent mechanical properties

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Neural Regeneration Research 2025年第7期20卷 2084-2094页

作者： Tong li Quhan Cheng Jingai Zhang Boxin liu Yu shi Haoxue Wang lijie Huang su Zhang Ruixin Zhang song Wang Guangxu Lu Peifu Tang Zhongyang liu Kai Wang Department of Orthopedics the Fourth Medical Center of Chinese PLA General HospitalBeijingChina Department of Training and sports Medicine Characteristic Medical Center of Chinese People’s Armed Police ForceTianjinChina Medical school of chinese PLA BeijingChina Key Laboratory of Bioactive Materials for the Ministry of Education College of Life SciencesNankai UniversityTianjinChina Department of Orthopedics Tianjin HospitalTianjinChina Department of Rehabilitation Medicine Er Quan Hospital of PAPWuxiJiangsu ProvinceChina

Autografting is the gold standard for surgical repair of nerve defects>5 mm in length;however,autografting is associated with potential complications at the nerve donor *** an alternative,nerve guidance conduits may be *** ideal conduit should be flexible,resistant to kinks and lumen collapse,and provide physical cues to guide nerve *** designed a novel flexible conduit using electrospinning technology to create fibers on the innermost surface of the nerve guidance conduit and employed melt spinning to align ***,we prepared disordered electrospun fibers outside the aligned fibers and helical melt-spun fibers on the outer wall of the electrospun fiber *** presence of aligned fibers on the inner surface can promote the extension of nerve cells along the *** helical melt-spun fibers on the outer surface can enhance resistance to kinking and compression and provide *** novel conduit promoted nerve regeneration and functional recovery in a rat sciatic nerve defect model,suggesting that it has potential for clinical use in human nerve injuries.

关键词： aligned fibers anti-kinking helical fibers nerve guidance conduit nerve regeneration peripheral nerve injury topological guidance

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The effect of cigarette smoke extract on thrombomodulinthrombin binding: an atomic force microscopy study

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science china(life sciences) 2012年第10期55卷 891-897页

作者： WEI YuJie ZHANG XueJie XU li yi shaoQiong li yi FANG XiaoHong liu huiliang Department of Cardiology the General Hospital of Chinese People's Armed Police ForcesBeijing 100039China beijing National Laboratory for Molecular sciences CAS Key Laboratory of Molecular Nanostructure and Nanotechnology Institute of ChemistryChinese Academy of SciencesBeifing 100190China beijing Institute of Biotechnology Academy of Military Medical SciencesBeijing 100071China

Cigarette smoking is a well-known risk factor for cardiovascular disease. smoking can cause vascular endothelial dysfunction and consequently trigger haemostatic activation and thrombosis. However, the mechanism of how smoking promotes thrombosis is not fully understood. Thrombosis is associated with the imbalance of the coagulant system due to endothelial dysfunction. As a vital anticoagulation cofactor, thrombomodulin (TM) located on the endothelial cell surface is able to regulate intravascular coagulation by binding to thrombin, and the binding results in thrombosis inhibition. This work focused on the effects of cigarette smoke extract (CsE) on TM-thrombin binding by atomic force microscopy (AFM) based single-molecule force spectroscopy. The results from both in vitro and live-cell experiments indicated that CsE could notably reduce the binding probability of TM and thrombin. This study provided a new approach and new evidence for studying the mechanism of thrombosis triggered by cigarette smoking.

关键词： cigarette smoke extract (CsE) thrombin thrombomodulin AFM single-molecule force spectroscopy protein-proteininteraction

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中药降乳散对乙烯雌酚诱发的大鼠泌乳素瘤防治作用的初步探讨

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武警医学 2001年第10期12卷 595-597页

作者：徐春李江源武警总医院内分泌科北京100039 解放军总医院内分泌科

目的　研究中药汤剂降乳散对乙烯雌酚 (DEs)诱发的大鼠泌乳素 (PRL)瘤的防治作用。方法　成年Wistar大鼠随机分 4组 ,对照组 (C组 )不做任何处理 ,其余三组 (E、D、Z组 )切除卵巢后皮下植入含有乙烯雌酚 (DEs)的硅胶管 ,其中两组分别用... 详细信息

目的　研究中药汤剂降乳散对乙烯雌酚 (DEs)诱发的大鼠泌乳素 (PRL)瘤的防治作用。方法　成年Wistar大鼠随机分 4组 ,对照组 (C组 )不做任何处理 ,其余三组 (E、D、Z组 )切除卵巢后皮下植入含有乙烯雌酚 (DEs)的硅胶管 ,其中两组分别用多巴胺受体激动剂诺果宁 (D组 )和中药降乳散 (Z组 )灌胃。 8周后处死动物 ,做垂体的免疫组化检查 ,用放免法测定外周血PRL水平 ,用RT -PCR方法测定雌激素受体α(ERα)在各组垂体组织中的表达。结果　DEs可使血清PRL水平、垂体重量和PRL阳性细胞计数明显增高 (P 1) ,多巴胺受体激动剂和中药能使上述指标明显下降 (P1)。ERα在各组大鼠垂体组织均有表达 ,其中在E、D、Z各组的表达水平分别比C组明显升高 (P 1) ,在E、D、Z各组间的表达水平无显著差别。结论　中药降乳散对DEs诱发的大鼠PRL瘤均具有一定程度的防治作用。

关键词：雌激素受体α 大鼠泌乳素瘤多巴胺受体激动剂中药药理

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Urgent Need for Contraceptive Education and services in chinese Unmarried Undergraduates:A Multi-campus survey

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Journal of Huazhong University of science and Technology(Medical sciences) 2011年第4期31卷 426-432页

作者：周远忠熊锦文李洁黄诗韵商学军刘国辉张玫玫尹平魏晟熊承良 Institute of Family Planning Tongji Medical CollegeHuazhong University of Science and Technology school of Public Health Zunyi Medical College Department of O&G Hubei Provincial Corps HospitalChinese People's Armed Police Forces Reproductive Medical Center Renmin Hospital of Wuhan University Department of Andrology Nanjing General Hospital of Nanjing Military Region Institute for Population and Family Planning of Chongqing City sexual Health Education Research Center Capital Normal University Department of Epidemiology School of Public HealthTongji Medical CollegeHuazhong University of Science and Technology

In order to ascertain prevalence rate of premarital sexual intercourse,unintended pregnancy and abortion,and evaluate associated factors of unintended pregnancy among undergraduates from all over china,the representative sample of unmarried undergraduates was obtained by using a multi-stage,stratified,probability cluster design,and data were collected by using a survey questionnaire.62 3.6 available responders were gained.11.6% of them acknowledged having experiences of premarital sexual intercourse（standardized prevalence rate of sexual intercourse was 13.8%）.3..5% of students active in premarital sex acknowledged undergoing unintended pregnancy.76.2% of pregnant students selected abortion to end *** students active in premarital sex,46.2% used contraception at the first sexual intercourse,28.2% replied ＂always＂ using contraception in sexual *** rate of using condoms,oral contraceptives（OCs）,and withdrawal among students who had used contraception was 52.0%,3..0%,and 27.2% respectively.＂No preparation for sex＂（40.3.）,＂pleasure decrement＂（3..1%）,＂won＇t-be-pregnancy in occasional sexual intercourse＂（3..2%） were their common excuses for using no *** identified risk factors for unintended pregnancy among students active in premarital sex by multivariate analysis were as follows：having no steady lover [having no steady lover vs having a steady lover：odds ratio（OR）,1.875;95% confidence interval（CI）,1.629-2.158],unaware of the course of conception（unaware vs aware：OR,2.023.95% CI,1.811-2.260）,considering abortion not endanger women＇s physical and mental health（no endangerment vs endangerment：OR,2.659;95% CI,2.265-3.121）,nonuse of contraception（never use vs always use：OR,1.682;95% CI,1.295-2.185）.Medical students were not less likely to experience an unintended pregnancy than nonmedical students（OR,1.111;95% CI,0.906-1.287）.The substantial proportion of unintended pregnancy among undergraduates indicates a need for convenient and targeted contra

关键词： students unsafe sex contraception unplanned pregnancy china

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CyclinD1、Rb和P16基因在脑星形细胞瘤中表达的研究

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武警医学 2003年第10期14卷 595-598页

作者：郑静晨李振洲杜继臣武警总医院北京100039 航天中心医院内二科北京100039

目的　通过检测脑星形细胞瘤组织中CyclinD1、Rb和P16基因蛋白表达 ,分析其表达变化与不同病理类型肿瘤的相关性。方法　选用 5 4例Ⅰ～Ⅳ级星形细胞瘤手术后存档蜡块标本为实验标本 ,应用流式细胞术 (FCM)分别定量检测Ⅰ～Ⅳ级星形细... 详细信息

目的　通过检测脑星形细胞瘤组织中CyclinD1、Rb和P16基因蛋白表达 ,分析其表达变化与不同病理类型肿瘤的相关性。方法　选用 5 4例Ⅰ～Ⅳ级星形细胞瘤手术后存档蜡块标本为实验标本 ,应用流式细胞术 (FCM)分别定量检测Ⅰ～Ⅳ级星形细胞瘤组织中CyclinD1、Rb和P16基因蛋白表达量。结果　CyclinD1蛋白的表达量随肿瘤恶性度的升高而显著增加 ,与病理组织学分级呈明显相关性 (P 1) ;Rb蛋白和P16蛋白表达量 (FI值 )在低恶性组升高 ,高恶性组降低 ,两组相比差异有显著性意义 (P 1)。结论　CyclinD1基因蛋白表达随星形细胞瘤恶性度升高而增强 ;Rb和P16在高恶性度星形细胞瘤均有不同程度的表达缺失。

关键词： CyclinD1基因 Rb基因 P16基因脑星形细胞瘤流式细胞术细胞周期

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53.外伤性肝破裂的诊断及治疗体会

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新疆医科大学学报 2009年第8期32卷 1118-1119页

作者：孙世安曹峻姚敏谷昊新疆武警总队医院新疆乌鲁木齐830091 新疆医科大学第一附属医院普外科新疆乌鲁木齐830011

目的:探讨外伤性肝破裂的诊断及治疗方法。方法:回顾性分析我院2003.1月～2007年12月间收治的53.外伤性肝破裂患者的临床资料。其中经保守治疗15例,手术治疗3.例。结果:治愈48例,5例死亡,死亡原因为失血性休克及多脏器功能衰竭。结论... 详细信息

目的:探讨外伤性肝破裂的诊断及治疗方法。方法:回顾性分析我院2003.1月～2007年12月间收治的53.外伤性肝破裂患者的临床资料。其中经保守治疗15例,手术治疗3.例。结果:治愈48例,5例死亡,死亡原因为失血性休克及多脏器功能衰竭。结论:外伤性肝破裂应及时诊断制订合理的治疗方案,准确评估患者的病情,选择正确的手术方式,积极处理合并伤,可降低病死率。

关键词：外伤性肝破裂诊断外科治疗

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慢性功能性便秘对老年人健康的影响及防治

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武警医学 2004年第7期15卷 506-509页

作者：金伟森李金锁杨绍勃金虎武警总医院肛肠外科北京100036 解放军装甲兵工程学院门诊部北京100028 解放军第二炮兵总医院北京100028

目的　研究慢性功能性便秘对老年人健康的影响及防治措施。方法　对 3.2例老年慢性功能性便秘患者的临床资料进行总结。结果　发现诱发和加重老年人慢性功能性便秘的临床因素包括性别、原发病及伴发病等。本组病例某些特定原发病存在明... 详细信息

目的　研究慢性功能性便秘对老年人健康的影响及防治措施。方法　对 3.2例老年慢性功能性便秘患者的临床资料进行总结。结果　发现诱发和加重老年人慢性功能性便秘的临床因素包括性别、原发病及伴发病等。本组病例某些特定原发病存在明显的性别差异 ,主要伴发病的发生率为冠心病 6 0 .5 4 %、糖尿病 2 8.6 1%、脑血管疾病 16 .87%、帕金森病6 .93. ,而且原发病或伴发病并发 3.以上 (包括 3. )分别为 6 5 .6 6 %和 5 9.3.%。结论　慢性功能性便秘严重影响了老年人的健康 ,深入探讨其特点及规律 ,采取积极的应对措施将极大地改善老年人的生活质量。

关键词：慢性功能性便秘老年人健康预防治疗

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