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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

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Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei li Feng Yu ZHAO Meng XING Chen Hao WANG Jie TIAN Feng Yu ZHANG Rui Qing LYU Xiao Na HAN Zhi Qiang WANG Yu Xin li hong yi SHEN Xin Xin MA Xue Jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results foryielded Kappa values of 0.839,0.815,and 0.856,respectively(Plizing M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

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基于4.3 THz量子级联激光器的微米级无损厚度测量

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红外与毫米波学报 2024年第3期43卷 354-358页

作者：李弘义谭智勇万文坚曹俊诚中国科学院上海微系统与信息技术研究所集成电路材料全国重点实验室上海200050 中国科学院大学材料科学与光电工程中心北京100049

构建和描述了一种用于获取硅片厚度的零差检测系统。利用4.3-THz激光束的传输相变与机械旋转台控制的入射角之间的关系,可以使用标准残差法精确推导被测样品的厚度值。结果表明,样品的厚度拟合值与光学显微镜的精确测量结果仅相差2.5~3... 详细信息

构建和描述了一种用于获取硅片厚度的零差检测系统。利用4.3-THz激光束的传输相变与机械旋转台控制的入射角之间的关系,可以使用标准残差法精确推导被测样品的厚度值。结果表明,样品的厚度拟合值与光学显微镜的精确测量结果仅相差2.5~3μm,实现了微米级精度的太赫兹无损厚度测量。实验验证了太赫兹量子级联激光器在非接触无损测量中的有效性。

关键词：太赫兹量子级联激光器零差探测无损检测非接触测量

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Crust and upper mantle structure in east China and sea areas

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Acta Seismologica Sinica(English Edition) 2001年第5期14卷 503-511页

作者： li hong-yi Institute of Crustal Dynamics China Seismological Bureau Beijing China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China

Eastern China and vicinal sea areas (98°-150°E, 5°-50°N) are located in the junction zone of Eurasian and Pacific plates, and it is also profoundly influenced by the collision between Indian and Eurasian plates. We utilized surface wave data recorded by 35 digital seismic stations in the area, extracted dispersion curves of fundamental-mode Rayleigh waves along 1252 paths by means of time-frequency analysis based on multiple-filter and other techniques. The study area was divided into a grid of 1°×1°, group velocity distributions of fundamental Rayleigh waves between 10-158 s were determined by Occam's inversion. These velocity distributions roughly depict the lateral variations of shear wave velocities in different depth ranges. With the determined pure path dispersions we further inverted for the S wave velocity structures along two profiles from eastern Tibet to Pacific Ocean and from South China Sea to Mongolia. It is found that the lateral heterogeneity is obvious to 400 km depth, and the velocity structures are correlated with tectonic units in the study area.

关键词： Rayleigh wave group velocity inversion crust-upper mantle velocity structure P313.2

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基于有限元理论的胶印机橡皮滚筒模态分析

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绿色包装 2024年第9期 25-28页

作者：李奕宏王燕北京印刷学院北京102600

胶印机橡皮滚筒作为胶印机的关键部件影响着印刷精度。本文基于有限元理论,对胶印机橡皮滚筒进行了模态分析。首先利用Solidworks对滚筒进行了几何建模,并且导入Ansys软件中进行静力学特性分析以及振动特性分析,得出了应力、应变云图、... 详细信息

胶印机橡皮滚筒作为胶印机的关键部件影响着印刷精度。本文基于有限元理论,对胶印机橡皮滚筒进行了模态分析。首先利用Solidworks对滚筒进行了几何建模,并且导入Ansys软件中进行静力学特性分析以及振动特性分析,得出了应力、应变云图、固有频率和模态振型图。静力学分析得出,最大等效应力值为280.61MPa,小于屈服极限强度370MPa,达到实际运用的要求。模态分析结果得出滚筒前三阶主要为滚筒中间弯曲变形,最大振型主要发生在中间部分;后三阶滚筒的扭转弯曲变形,最大振型主要发生在滚筒两端和轴头。得到橡皮滚筒的一阶固有频率为380.08Hz,远远低于橡皮滚筒临界转速,为胶印机橡皮滚筒的可靠性分析提供了计算依据。

关键词：胶印机橡皮滚筒有限元模态分析

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Two-Variable Hermite Polynomial Excitation of Two-Mode Squeezed Vacuum State as Squeezed Two-Mode Number State

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Communications in Theoretical Physics 2008年第10期50卷 965-970页

作者： HU li-Yun FAN hong-yi Department of Physics Shanghai Jiao Tong University Shanghai 200030 China

We find that the squeezed two-mode number state is just a two-variable Hermite polynomial excitation of thetwo-mode squeezed vacuum state (THPES).We find that the Wigner function of THPES and its marginal distributionsare just related to two-variable Hermite polynomials (or Laguerre polynomials) and that the tomogram of THPES canbe expressed by one-mode Hermite polynomial.

关键词： Two variable Hermite polynomial excitation state Wigner function marginal distribution tomgram

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Quantum Optical Squeezing Transform for Generalizing Fractional Fourier Transform

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Communications in Theoretical Physics 2008年第10期50卷 951-954页

作者： HU li-Yun FAN hong-yi Department of Physics Shanghai Jiao Tong University Shanghai 200030 China

By establishing the relation between the optical scaled fractional Fourier transform （FFT） and quantum mechanical squeezing-rotating operator transform, we employ the bipartite entangled state representation of two-mode squeezing operator to extend the scaled FFT to more general cases, such as scaled complex FFT and entangled scaled FFT. The additiyity and eigenmodes are presented in quantum version. The relation between the scaled FFT and squeezing-rotating Wigner operator is studied.

关键词： squeezing transform scaled fractional Fourier transform IOWP technique

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Query routing mismatch alleviation architecture for P2P file lookup in MANETs

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The Journal of China Universities of Posts and Telecommunications 2011年第4期18卷 111-117页

作者： MEI Jing-qing JI hong li yi Key Laboratory of Universal Wireless Communications Ministry of Education Beijing University of Posts and Telecommunications Beijing 100876 China

A cross-layer optimized query routing mismatch alleviation （QRMA） architecture is proposed to mitigate the problem of query routing mismatch （QRM） phenomenon between the structured peer to peer （P2P） overlay and the routing layer in mobile Ad-hoc networks （MANETs）, which is an important issue that results in the inefficiency of lookup process in the system. Explicated with the representative Chord protocol, the proposal exploits the information of topologic neighbors in the routing layer of MANETs to find if there is any optimized alternative next hop in P2P overlay during conventional lookup progress. Once an alternative next hop is detected, it will take the shortcut to accelerate the query procedure and therefore alleviate the QRM problem in scalable MANETs without any assistance of affiliation equipments such as GPS device. The probability of finding out such an alternative node is formulated and the factors that could increase the chance are discussed. The simulation results show that the proposed architecture can effectively alleviate the QRM problem and significantly improve the system performance compared with the conventional mechanism.

关键词： query routing mismatch P2P overlay Chord MANETs

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金融开放视角下跨周期与逆周期有机结合“双支柱”调控框架研究

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哈尔滨师范大学社会科学学报 2024年第3期15卷 100-104页

作者：谭小珂李义红重庆移通学院淬炼商学院重庆合川401520

金融开放带来经济发展的同时,也伴随着风险的增加,如何在开放中保持金融稳定,成为当前需要面对的重要问题。为此,本研究提出一种金融开放视角下跨周期与逆周期有机结合“双支柱”调控框架。该框架将货币政策和宏观审慎政策作为两大支柱... 详细信息

金融开放带来经济发展的同时,也伴随着风险的增加,如何在开放中保持金融稳定,成为当前需要面对的重要问题。为此,本研究提出一种金融开放视角下跨周期与逆周期有机结合“双支柱”调控框架。该框架将货币政策和宏观审慎政策作为两大支柱,综合运用逆周期调节和跨周期调节手段,在逆周期调节方面,该框架通过货币政策等手段,对经济进行短期调控,以平抑经济周期波动;跨周期调节方面,通过宏观审慎政策等手段,引导金融机构和市场形成合理的运行机制,防范化解长期性、系统性风险。

关键词：金融开放视角跨周期逆周期 “双支柱”调控必要性理论基础框架设计

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Multi-path routing based on load-balance for cognitive packet networks

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The Journal of China Universities of Posts and Telecommunications 2011年第5期18卷 71-75页

作者： CHEN liang JI hong li yi li Xi College of Information and Commumieation Engineering Beijing University of Posts and Telecommunications Beijing 100876 China

Quality of service （QoS） routing algorithms have been hardly discussed in the scientific community, most previous work on QoS routing concentrates on the performance of the single route. Cognitive packet network （CPN） has been designed for providing QoS routing. In this paper, to balance the loads among networks, we present a multi-path routing algorithm based on load-balance （MPRLB）, which is carried out in two steps. The algorithm with low computational complexity is firstly applied to establish multi path routing for each source and destination node pairs （SD-pair） nodes in the network. Then, we propose the hopfield neural network algorithm, which is applied to improve the efficiency of the flow deviation method for fast flow allocation among the links of the network based on load balance. Extensive simulation results demonstrate that the proposed scheme significantly improves the performance compared with the existing scheme that ignores load balancing.

关键词： hopfield neural network CPN multi-path routing

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交互共存:从甘青“花儿”看多民族的交往交流交融

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青海民族大学学报（社会科学版） 2024年第2期50卷 52-59页

作者：蔡秀清周毛吉李以泓西藏大学西藏拉萨850000

“花儿”是生长在西北大地上的一朵艺术奇葩,蕴含丰厚的历史积淀和文化内涵。“花儿”是西北地区多民族集体的智慧结晶,也是多民族交往交流交融的文化自信和文化认同的基质。在中华文化的大熔炉中,“花儿”是其中的瑰宝,它的旋律流淌过... 详细信息

“花儿”是生长在西北大地上的一朵艺术奇葩,蕴含丰厚的历史积淀和文化内涵。“花儿”是西北地区多民族集体的智慧结晶,也是多民族交往交流交融的文化自信和文化认同的基质。在中华文化的大熔炉中,“花儿”是其中的瑰宝,它的旋律流淌过岁月的文化长河,歌词浸染着各民族文化的鲜明色彩,以摄魂夺魄的艺术魅力,涤荡着千千万万民众的心灵,真正彰显了各民族交融共生的社会理路。文章以传唱在甘青地区最为广泛的河州“花儿”为例,以交互共存的文化特质为切入点,探讨甘青“花儿”中呈现的多民族交往交流交融,以期为铸牢中华民族共同体意识提供养分,为实现中华民族伟大复兴凝聚精神力量。

关键词：甘青地区多民族河州“花儿” 交往交流交融

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