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Foci of Future Studies on Abiotic Stress Tolerance of Maize in the Era of Post-Genomics

Journal of Integrative Agriculture 2012年第8期11卷 1236-1244页

作者： li you-zhi FAN Xian-wei liAO Jiang-xiong State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Ministry of Science and Technology/Key Laboratory of Microbial and Plant Genetic Engineering Ministry of Education/College of Life Science and Technology Guangxi University Guangxi 530005 P.R. China Sugarcane Research Center Chinese Academy of Agriculture Sciences Guangxi 530007 P.R.China

Genetic and metabolic engineering approaches are powerful tools for improving the tolerance of maize to abiotic stresses because they are faster and can afford greater control over agronomically useful traits. However, in-depth understanding of the molecular mechanisms controlling response to abiotic stresses is the prerequisite for successful implementation of these strategies. A great flaw to dissect the biological mechanisms by genome sequencing is that genome sequencing approach could not reflect real-time molecular actions of plants especially under the stresses because the living organisms rarely live in unchanging environments. Post-genomics such as transcriptomics, metabolomics, and proteomics can generate knowledge that is closer to the biological processes. With the development of post-genomics, it can be expected that voluminous data will be generated. This paper proposes that future research on maize stress tolerance in the era of post-genomics should focus on metabolomics and proteomics; stress tolerance of whole plant rather than individual tissues or organs; coordination of expression of genes among tissues; characterization of promoters of stress-responsive genes; interrelation between mechanisms for tolerance to, and growth recovery from the stress; hexose metabolism as well as the glycolysis pathway; and foundation genotypes.

关键词： maize abiotic stresses research foci era of post-genomics

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Anti-Insect Activity of the Methanol Extracts of Fern and Gymnosperm

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Agricultural Sciences in China 2010年第2期9卷 249-256页

作者： HUANG Su-qing ZHANG zhi-xiang li you-zhi li Yu-xia XU Han-hong Key Laboratory of Natural Pesticide ＆ Chemical Biology Ministry of Education/South China Agricultural University Guangzhou 510642 P.R. China College of Bio-Safety Science and Technology Hunan Agricultural University Changsha 410128 P.R.China

With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern to their particular status in plant taxology and co-evolution are of great significance for application as new pesticides due with insects. In this study, methanol extracts were distilled from wild fern and gymnosperm of 54 species of 22 families collected from Shiwandashan Mountain in Guangxi Zhuang Autonomous Region of China. Insecticidal activities of the methanol extracts were assayed on both adult house fly （Musca domestica） and mosquito （Aedes albopictus）. Significant insecticidal activities were exhibited in the methanol extracts of five species, Cupressusfunebris （leaves and stems）, Cycas acuminatissima （roots）, Keteleeria fortunei （leaves and stems）, Onychium japonicum （whole plant）, and Pinus taiwanensis var. Darning shanensis （leaves and stems）. Applications of the extracts of these plants resulted in higher than 50% mortalities in 4th instar larvae of A. albopictus at 24 h after treatment. The methanol extracts from 13 species possessed insecticidal activities against the adult of M. domestica at 48 h after treatment with higher than 90% mortalities. The extensive screening results showed that these fern and gymnosperm were highly potential to be botanical insecticides. The findings provide a feasible and valuable basis for further investigation.

关键词： plant extracts anti-insect activity Musca domestica Aedes albopictus

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Functions and Application of the AP2/ERF Transcription Factor Family in Crop Improvement

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Journal of Integrative Plant Biology 2011年第7期53卷 570-585页

作者： Zhao-Shi Xu Ming Chen lian-Cheng li you-zhi Ma National Key Facility of Crop Gene Resources and Genetic Improvement (NFCRI) Key Laboratory of Crop Genetics and Breeding Ministry of Agriculture Institute of Crop Science Chinese Academy of Agriculture Sciences (CAAS) Beijing 100081 China

Plants have acquired sophisticated stress response systems to adapt to changing environments. It is important to understand plants＇ stress response mechanisms in the effort to improve crop productivity under stressful conditions. The AP2/ERF transcription factors are known to regulate diverse processes of plant development and stress responses. In this study, the molecular characteristics and biological functions of AP2/ERFs in a variety of plant species were analyzed. AP2/ERFs, especially those in DREB and ERF subfamilies, are ideal candidates for crop improvement because their overexpression enhances tolerances to drought, salt, freezing, as well as resistances to multiple diseases in the transgenic plants. The comprehensive analysis of physiological functions is useful in elucidating the biological roles of AP2/ERF family genes in gene interaction, pathway regulation, and defense response under stress environments, which should provide new opportunities for the crop tolerance engineering.

关键词： AP2/ERF gene regulation signal pathway stress tolerance transgenic plant

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The Efficiency of Southern rice black-streaked dwarf virus Transmission by the Vector Sogatella furcifera to Different Host Plant Species

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Journal of Integrative Agriculture 2012年第4期11卷 621-627页

作者： li you-zhi CAO Yang ZHOU Qian GUO Hai-ming OU Oao-cai Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests College ofBiosafety ＆ Technology Hunan Agricultural University Changsha 410128 P.R. China Hunan Provincial Plant Protection Station Changsha 410650 P.R.China National Research Center of Engineering ＆ Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University Changsha 410128 P.R.China

Southern rice black-streaked dwarf disease is a new rice disease that severely affects rice production in South *** understand transmission capacity of the vector Sogatella furcifera to Southern rice black-streaked dwarf virus（SRBSDV） among different host plant species,potential host plants of SRBSDV collected from the diseased rice field and/or adjacent to the field in Hunan Province,China,were determined by RT-PCR,and the transmission rates of SRBSDV by *** among different host plant species were *** results showed that host plants of SRBSDV in the rice fields were five of family Gramineae（Oryza sativa,Echinochloa crusgalli,Zea mays,Paspalum distichum,Alopecurus aequali） and two of family Cyperaceae（Juncellus serotinus and Cyperus difformis）.*** could not transmit SRBSDV between gramineous plants and cyperaceous plants,and could not transmit SRBSDV between the gramineous plants,*** and *** as ***,SRBSDV could be transmitted by *** within gramineous *** could transmit SRBSDV between interspecies among three species plants（***,*** and ***）,and between *** and *** 15,20,25,30,and 35°C,both macropterous and brachypterous adult of *** could transmit SRBSDV from the plants（e.g.,***,*** and ***） infected with SRBSDV to rice *** transmission rates were first increased and then decreased with the increase of *** adults transmitted SRBSDV from the viruliferous ***,*** and rice plants to the healthy rice seedlings,and the infected rates of rice seedlings were 26.2,18.8 and 23.7% at 15°C,56.6,64.6 and 53.6% at 25°C,and was 11.2,10.2 and 7.3% at 35°C,*** capacity of brachypterous adults was significantly higher than that of macropterous adults at 15,20 and 25°C（P0.05）,while transmission capacity of brachypterous adults was relatively lower compared with that of macropterous ones

关键词： Sogatella furcifera Southern rice black-streaked dwarf virus host plants transmission efficiency

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Characterization of the Promoter of a Homolog of Maize MADS-Box Gene m18

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Journal of Integrative Agriculture 2014年第11期13卷 2330-2345页

作者： QIN Hui-juan PAN Hong FAN Xian-wei WU Qiao li you-zhi Key Laboratory for Microbial and Plant Genetic Engineering Ministry of Education/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Ministry of Science and Technology/College of Life Science and Technology Guangxi University

Maize （Zea mays L.） is one of the world’s major food crops, and often suffers from tremendous yield loss caused by abiotic stresses. The MADS-box genes are known to play versatile roles in plants, controlling plant responses to multiple abiotic stresses. However, understanding of regulation of their expressions by the conventional loss-of-function approach is very dififcult. So far, regulation of MADS-box gene expression is little known. The best approach to retrieve expression regulation of this category of genes is to characterize expression of their promoters. In this study, the promoter of a homolog （GenBank accession no. EC864166） of maize MADS-box gene m18 was cloned by way of genome-walking PCR, named Pro66. Predicative analysis indicated that Pro66 contains more than one TATA box and multiple cis-acting environmental conditions-responsive elements （ECREs）. Pro66 could drive expression of theβ-glucuronidase （GUS）-encoding gene in maize, and heterologous expression of GUS in red pepper stressed by water deifcit, salt, copper, iron deifciency, heat, cold, and grown under short and long photoperiods, echoing predicative ECREs. Conclusively, maize MADS-box gene m18 likely plays versatile functions in maize response to multiple abiotic stresses due to the promoter with multiple cis-acting elements. The complex arrangement of multiple cis-acting elements in the promoter features meticulously regulated expression of m18. The results give informative clues for heterologous utilisation of the promoters in monocot and dicot species. The copy of the ECREs and heterologous expression of the promoter in dicot species are also discussed.

关键词： maize MADS-box gene promoter stress transcription

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Synthesis of lithium-cobalt Oxides by Sol-gel Method and their Cathodical Discharge Behavior at 500 ℃

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Journal of Central South University 2000年第2期12卷 57-60页

作者： li zhi you,HUANG Bai yun,ZHU Yong li,liU zhi jian,QU Xuan hui (State Key Laboratory for Powder Metallurgy, Central South University of Technology, Changsha 410083, China) 1. State Key Laboratory for Powder Metallurgy Central South University of Technology 410083 Changsha China

Three kinds of lithium cobalt oxides with lithium to cobalt atomic ratios of 0.6, 0.8 and 1.0 respectively were prepared using nitrates and citric acid by sol gel process. During the baking of the dry gel powder, two exothermic peaks occur, followed by the formation of li 2O and Co 3O 4 and then the combination of liCoO 2, which is testified by DTA and XRD. The powder formed from an alkaline sol is finer than that from the acidic one. There are a single phase intercalation of lithium ion and an electrochemical reduction reaction for higher valence cobalt ion when simulating the discharging process of li B/liCl KCl/lithium cobalt oxides at 500 ℃, and the voltage and specific capacity are not sensitive to the initial ratios of lithium to cobalt.

关键词： sol gel liCoO 2 thermal battery cathode Document code:

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Research and Development of P2P Worms

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Journal of Electronic Science and Technology 2011年第3期9卷 202-210页

作者： li you zhi-Guang Qin School of Computer Science and Engineering University of Electronic Science and Technology of China Chengdu 611731 China

With the development and the application of many popular peer-to-peer （P2P） systems such as eMule and BitTorrent, worms probably employ the features of these P2P networks to put them at risk. Some features, such as the local routing table and the application routing mechanism, are helpful to quickly distribute the P2P worms into the networks. This paper aims to give a comprehensive survey of P2P worms. The definition and the classification of P2P worms are discussed firstly. Then, the research and development of P2P worms, including experimental analysis, propagation modeling, and defensive approaches, are addressed and analyzed in detail.

关键词： Index Terms---Peer-to-peer (P2P) P2P worm worm propagation model.

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Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第2期8卷 88-97页

作者： JIN Qing-chao DONG Hai-tao PENG you-liang CHEN Bao-shan SHAO Jing DENG Ye DAI Cheng-en FANG Yong-qi LOU Yi-chun li you-zhi li De-bao Bioinformatics and Gene Network Research Group School of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Key Laboratory of Molecular Plant Pathology Ministry of Agriculture China Agricultural University Beijing 100094 China Laboratory of Subtropical Bioresource Conservation and Utilization Guangxi University Nanning 530004 China

Appressorium is an infection structure of the phytopathogenic fungus Magnaporthe grisea. Analysis of gene expression profiles ofappressorium development provides insight into the molecular basis of pathogenicity and control of this fungal plant disease. A cDNA array representing 2927 unique genes based on a large EST （expressed sequence tag） database ofM. grisea strain Y34 was constructed and used to profile the gene expression patterns at mycelium and appressorium maturation stages. Compared with mycelia, 55 up-regulated and 22 down-regulated genes were identified in mature appressoria. Among 77 genes, 16 genes showed no similarity to the genome sequences of M. grisea. A novel homologue of peptidyl-prolyl cis-trans isomerase was found to be expressed at low-level in mature appressoria of M. grisea. The results indicated that the genes such as pyruvate carboxylase, phospholipid metabolism-related protein and glyceraldehyde 3-phosphate dehydrogenase involved in gluconeogenesis, lipid metabolism and glycolysis, showed differential expression in mature appressoria. Furthermore, genes such as PTHll, beta subunit of G protein and SGTI involved in cell signalling, were expressed differentially in mature appressoria. Northern blot analysis was used to confirm the cDNA array results.

关键词： Magnaporthe grisea Mature appressoria cDNA array Gene expression profile

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Cathodic electrophoretic deposition of α-Fe_2O_3 coating

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Journal of Central South University 2015年第6期22卷 2027-2035页

作者：马莉常通李小斌李志友张斗周科朝 School of Metallurgy and Environment Central South University State Key Laboratory of Powder Metallurgy (Central South University)

Submicro α-Fe2O3 coatings were formed using electrophoretic deposition(EPD) technique in aqueous media. The zeta potentials of different α-Fe2O3 suspensions with different additives were measured as a function of p H to identify the optimum suspension condition for deposition. Electrophoretic depositions of α-Fe2O3 coatings under different applied electric fields and deposition time were studied and the effects of applied voltages and deposition time on deposition rates and thicknesses were investigated. The particle packing densities of the deposits at various applied voltages and deposition time were also analyzed by a scanning electron microscope(SEM). The results show that crack-free α-Fe2O3 coatings with uniform microstructure and good adherence to the nickel substrates are successfully obtained. Electrophoretic deposited α-Fe2O3 coating from aqueous suspension is a feasible, low-cost and environmental friendly method.

关键词： electrophoretic deposition Fe2O3 coating aqueous solutions zeta potential

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Moclobemide up-regulates proliferation of hippocarnpal progenitor cells in chronically stressed mice

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Acta Pharmacologica Sinica 2004年第11期 24-28页

作者： Yun-feng li, you-zhi ZHANG, Yan-qin liU, Heng-lin WANG, li YUAN, zhi-pu LUOBeijing Institute of Pharmacology and Toxicology, Beijing 100850 Department of Anesthesiology, 309 Hospital of Beijing, Beijing 100091, China

AIM: To explore the action mechanism of antidepressants. METHODS: The PC12 cell proliferation was detected by flow cytometry,. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS: Treatment with N-methylaspartate (NMDA) 600μmol/L for 3 d significantly decreased the percentage of S-phase in PC12 cells, while in the presence of classical antidepressant, moclobemide (MOC) 2 and 10μmol/L, the percentage in S-phase increased. Furthermore, the proliferation of progenitor cells in hippocampal dentate gyrus (subgranular zone), as well as the level of BDNF in hippocampus significantly decreased in chronically stressed mice, while chronic administration with MOC 40 mg/kg (ip) up-regulated the progenitor cell proliferation and BDNF level in the same time course. CONLUSION: Up-regulation of the proliferation of hippocampal progenitor cells is one of the action mechanisms for MOC, which may be closely related to the e

关键词： antidepressants moclobemide neurogenesis stress brain-derived neurotrophic factor N-methylaspartate

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