Moclobemide up-regulates proliferation of hippocarnpal progenitor cells in chronically stressed mice

作     者：Yun-feng LI, You-zhi ZHANG, Yan-qin LIU, Heng-lin WANG, Li YUAN, Zhi-pu LUOBeijing Institute of Pharmacology and Toxicology, Beijing 100850 Department of Anesthesiology, 309 Hospital of Beijing, Beijing 100091, China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第11期

页      面：24-28页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China(No30300419)Natural Science Foundation of Beijing City(No7042052) 

主　　题：antidepressants moclobemide neurogenesis stress brain-derived neurotrophic factor N-methylaspartate 

摘      要：AIM: To explore the action mechanism of antidepressants. METHODS: The PC12 cell proliferation was detected by flow cytometry,. The proliferation of hippocampal progenitor cells and level of brain-derived neurotrophic factor (BDNF) were measured by immunohistochemistry. RESULTS: Treatment with N-methylaspartate (NMDA) 600μmol/L for 3 d significantly decreased the percentage of S-phase in PC12 cells, while in the presence of classical antidepressant, moclobemide (MOC) 2 and 10μmol/L, the percentage in S-phase increased. Furthermore, the proliferation of progenitor cells in hippocampal dentate gyrus (subgranular zone), as well as the level of BDNF in hippocampus significantly decreased in chronically stressed mice, while chronic administration with MOC 40 mg/kg (ip) up-regulated the progenitor cell proliferation and BDNF level in the same time course. CONLUSION: Up-regulation of the proliferation of hippocampal progenitor cells is one of the action mechanisms for MOC, which may be closely related to the e