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Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin

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Chinese Chemical Letters 2008年第6期19卷 707-710页

作者： li wen Shao Chao qing Dong Xiang Yi Huang Ji Cun Ren College of Chemistry ＆ Chemical Engineering Shanghai Jiaotong University Shanghai 200240 China

In this work, the capillary electrophoresis mobility shift assay （CEMSA） was first adopted to study the interaction of protein with quantum dots （QDs）. In this study, bovine serum albumin （BSA） and CdTe QDs were used as model samples. We observed that BSA was facilely adsorbed to CdTe QDs surface, and the QD-BSA complex was formed by a 1：1 stoichiometric ratio. A value of 2.17 4-0.27 × 10^6 mol^-1 L^-1 （at 25 ℃） for the association constant was obtained by CEMSA.

关键词： CdTe quantum dots Bovine serum albumin Capillary electrophoresis mobility shift assay Interaction

Blocking effects of siRNA on VEGF expression in human colorectal cancer cells

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World Journal of Gastroenterology 2010年第9期16卷 1086-1092页

作者： Yu Yin,li-Yu Cao,wen-qing Wu,Hao li,Yan Jiang,Hong-Fu Zhang,Department of Pathology,Anhui Medical University,Hefei 230032,Anhui Province,China wen-qing Wu,Department of Pathology,Anhui Provincial Hospital,Hefei 230001,Anhui Province,China Department of Pathology Anhui Medical University Hefei 230032 Anhui Province China.

AIM:To investigate the expression of vascular endothelial cell growth factor (VEGF) and its receptors Fmslike tyrosine kinase 1 (FLT-1) and fetal liver kinase 1 (FLK-1) in colorectal carcinoma (CRC),and the blocking effects of small interfering RNAs (siRNAs) on VEGF expression in human colorectal cancer HCT116 ***:Immunohistochemical staining for VEGF,FLT-1 and FLK-1 proteins was performed in 82 cases of CRC and 14 normal colorectal mucosae.A siRNA targeting VEGF was synthesized and transfected into HCT116 cells using lipofectamine *** staining and Western blotting analyses were performed to detect the expression of VEGF *** suppressive effect of the siRNA on cell proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltertrazolium bromide (MTT) *** apoptosis was detected using flow cytometry (FCM).RESULTS:The expression of VEGF,FLT-1 and FLK-1 in tumor tissues was significantly higher than that in normal tissues (P=0.008,P=0.000,P=0.000).The expression of VEGF was positively correlated with both lymph node metastasis and clinical stage (P=0.009 and P=0.025,respectively).Immunocytochemistry showed that the expression of VEGF was weakly positive and Western blotting indicated a significant reduction in VEGF-siRNA cell protein ***-siRNA cell growth inhibition was assessed by the MTT assay,and the tumor cell proliferation rate was significantly different at 24,48,and 72 h after *** results showed that the VEGF-siRNA group had an apparent aneuploid ***:VEGF,FLT-1 and FLK-1 are associated with colorectal *** silencing of the VEGF gene suppresses proliferation,and induces apoptosis in HCT116 *** results suggest that VEGF may be a new gene therapy target for colorectal cancer.

关键词： Colorectal carcinoma Vascular endothelial cell growth factor Fms-like tyrosine kinase 1 Fetal liver kinase 1 Small interfering RNA

Radioimmunotherapy of carcinoma of colon with [131I]-labeled recombinant chimeric monoclonal antibodies to carcinoembryonic antigen

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Acta Pharmacologica Sinica 2005年第10期26卷 1259-1264页

作者： Qiu-jun LU~1, Guang-xing BIAN, Yuan-yuan CHEN, Min ZHANG, Shao-ming GUO, li-qing wen Institute of Radiation Medicine, Beijing 100850, China Institute of Radiation Medicine Beijing China

Aim: To study the distribution of [131I]-labeled anti-CEA MoAbs and its therapeutic effect on the human colonic cancer model in nude mice. Methods: A nude mice model of human colonic cancer was established. [~131I]-labeled anti-CEA MoAbs were injected intravenously into mice. The distribution of the MoAbs was then determined and the effect of RIT on human colonic cancer was observed. Results: The [131I]-labeled anti-CEA MoAbs had a specific distribution after injection. Tumor/non-tumor ratios for [131I]-labeled anti-CEA MoAbs were 10-20 times higher than [131I]-labeled IgG 96 h after injection. Thirty days after injection, significant inhibition of the volume and weight of tumor was observed in the treated mice compared with the control. The tumor growth inhibition rate of 3,1 mCi/kg CEA MoAbs group (LS180, LS174T, SW1116) was 47.8%～64.0%. This was 69.6%-78.6% in the 6.25 mCi/kg CEA MoAbs group, and 81.8%-86.2% in the 12.5 mCi/kg [131I]-labeled anti-CEA MoAbs group. The plasma CEA level was alo lower in treated mice. Conclusion: The results indicate that [131I]-labeled anti-CEA MoAbs can be effective in RIT on colonic cancers.

关键词： humanized chimeric recombinant monoclonal antibody radioimmunotherapy colonic cancer nude mice

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基于随机性特征的加密和压缩流量分类

吉林大学学报（工学版） 2021年第4期51卷 1375-1386页

作者：李光松李文清李青信息工程大学网络空间安全学院郑州450001 信息工程大学信息系统工程学院郑州450001

当网络传输数据应用加密或压缩算法后,其载荷数据均呈现出较强的随机性,利用现有的流量检测方法,很难将加密和压缩流量有效区分。针对上述问题,基于加密数据与压缩数据随机性的差异性特征,提出了ECF特征集,在不依赖网络传输协议、数据... 详细信息

当网络传输数据应用加密或压缩算法后,其载荷数据均呈现出较强的随机性,利用现有的流量检测方法,很难将加密和压缩流量有效区分。针对上述问题,基于加密数据与压缩数据随机性的差异性特征,提出了ECF特征集,在不依赖网络传输协议、数据包头、压缩标识等信息的情况下,使用当前主流机器学习算法构建分类模型,实现了有效的加密和压缩流量分类。实验测试表明,本文方法在分类精度上优于现有分类方法,并且具有很好的泛化性和迁移性。

关键词：流量分类加密流量压缩流量机器学习

Effect of Electro-Acupuncture on S-100 Protein Expression of Nerve Fibers in the Repair Process of Transected Sciatic Nerve in Rats

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Journal of Acupuncture and Tuina Science 2007年第1期5卷 10-13页

作者：李庆雯朱忠春(Translator) Tianjin Institute of Physical Education Tianjin 300193 P.R. China 不详

Objective： To observe the effects of electro-acupuncture on S-100 protein expression of nerve fibers on the regeneration process of transected sciatic nerve of rats, so as to explore the partial mechanism of electro-acupuncture treatment for peripheral nerve injury. Methods： Following rat＇s sciatic nerve transection, the nerve regeneration chamber model was established, then the rat was given electro-acupuncture stimulation at the frequency of 5 Hz and frequency of 100 Hz, and the immunohistochemical staining method and image analyzing semi-quantitative technique were adopted to determine the percentage of S-100 protein area, hence to observethe effect of electro-acupuncture on the repair process of transected sciatic nerve of rat and the S-100 protein expression. Results： Electro-acupuncture could significantly potentiate the S-100 protein expression of Schwann Cells （SC） in the repair process of transected sciatic nerve. At the 14th week, 17th week and 20th week after operation, the percentages of S-100 protein area were more obviously increased in 5 Hz electro-acupuncture group and 100 Hz electro-acupuncture group than in model group, with significant differences （P〈0.05）. Especially, the better effect was obtained in 5 Hz electro-acupuncture group. Judging from the dynamic treatment, it is very vital to be treated early. Conclusion： Electro-acupuncture is an effective method for the treatment of peripheral nerve injury, and its therapeutic effect is related to the improvement of SC proliferation.

关键词： Electro-acupuncture Nerve Fibers/Injuries S- 100 protein

纳米MnO_2的固相合成及其电化学性能研究(Ⅱ)纳米V-MnO_2的电化学性能

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高等学校化学学报 1999年第10期20卷 1584-1588页

作者：夏熙李娟李清文新疆大学应用化学研究所乌鲁木齐830046

用循环伏安（ＣＶ）和恒流放电及充放电技术测试了固相方法合成的纳米γ－ＭｎＯ２的电化学性能．结果表明，纳米γ－ＭｎＯ２在碱性电解液中具有优良的电化学活性．纳米样品在－０．２Ｖ之后极化程度变小，电位下降的趋势明... 详细信息

用循环伏安（ＣＶ）和恒流放电及充放电技术测试了固相方法合成的纳米γ－ＭｎＯ２的电化学性能．结果表明，纳米γ－ＭｎＯ２在碱性电解液中具有优良的电化学活性．纳米样品在－０．２Ｖ之后极化程度变小，电位下降的趋势明显减慢．重负荷放电时，纳米微粒样品显示出更大的优势，放电容量大幅度增加．ＣＶ图中显示，纳米ＭｎＯ２的峰电流明显大于国际１号电解二氧化锰样品（记为Ｓ）的峰电流，表明在纳米样品中，质子、电子的嵌入和脱出以及Ｍｎ（Ⅲ）。

关键词：纳米材料二氧化锰固相合成

纳米MnO_2的固相合成及其电化学性能的研究(Ⅰ)──纳米γ-MnO_2的合成及表征

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高等学校化学学报 1999年第9期20卷 1434-1437页

作者：李娟夏熙李清文新疆大学应用化学研究所乌鲁木齐830046

用醋酸锰分别与柠檬酸、草酸和8-羟基喹啉在室温及低温下发生固相反应生成锰配合物，经热分解和酸处理等手段最终制备出纳米γ－MnO2用元素分析、化学分析、X射线衍射、热重／差热分析和透射电镜等测试手段对固相反应的可行性作了讨论... 详细信息

用醋酸锰分别与柠檬酸、草酸和8-羟基喹啉在室温及低温下发生固相反应生成锰配合物，经热分解和酸处理等手段最终制备出纳米γ－MnO2用元素分析、化学分析、X射线衍射、热重／差热分析和透射电镜等测试手段对固相反应的可行性作了讨论，并对合成的样品进行了表征．从TEM照片上看，产物粒子呈球形，粒径多为20～30nm．酸处理可大大提高样品的氧化度，但酸化过程中粒子出现团聚现象，粒子形貌也有所改变，似有取向生长的趋势．文中还讨论了低温条件下利用固相反应制备纳米材料的原因．

关键词：固相合成纳米材料二氧化锰电极材料

高应变率下预制单节理岩石SHPB劈裂试验能量耗散分析

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岩土工程学报 2017年第7期39卷 1336-1343页

作者：李淼乔兰李庆文北京科技大学土木与资源工程学院北京100083

应用SHPB试验装置研究预制单节理岩石的能量耗散关系。使用SHPB试验系统,对高径比为0.5的完整花岗岩试样及预制单节理花岗岩试样进行高应变率下的冲击劈裂试验。在相同驱动气压下,改变加载方向与节理间的夹角,完成高应变率相同入射能下... 详细信息

应用SHPB试验装置研究预制单节理岩石的能量耗散关系。使用SHPB试验系统,对高径比为0.5的完整花岗岩试样及预制单节理花岗岩试样进行高应变率下的冲击劈裂试验。在相同驱动气压下,改变加载方向与节理间的夹角,完成高应变率相同入射能下的冲击劈裂试验。对SHPB系统中的入射能、反射能、透射能及试样吸收能的时程变化规律进行了分析;从能量角度出发,分析冲击荷载作用下单节理岩石的能量耗散规律及其各向异性特征。结果表明:高应变率下,完整花岗岩试样在冲击劈裂试验中的吸收能随平均应变率增加而增加,表现出显著的应变率相关性;预制单节理岩石与加载方向之间夹角对破坏模式的影响明显,节理试样产生3种破坏模式:(1)穿越节理面的劈裂破坏;(2)沿节理岩石层面的滑移破坏;(3)劈裂与滑移破坏共同作用下的破坏。在入射能基本相同,入射时间较长时节理岩石试样吸收能较入射时间较短时的吸收能大。动态劈裂试验中,节理试样的吸收能随节理角度变化(0°~90°)近似呈U型。研究成果可为节理岩石动态力学性能研究提供参考。

关键词： SHPB 冲击劈裂试验单节理岩石能量耗散各向异性

Eco county construction in China

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Journal of Environmental Sciences 1999年第3期11卷 284-289页

作者： li wen hua, Min qing wen Commission for Integrated Survey of Natural Resources, Chinese Academy of Sciences, Beijing 110044, China Miao Ze wei Department of Systems Ecology, Research Center for Eco Environmental Sciences, Chinese Academy of 中科院自然资源综合考察委员会北京 100044 中科院自然资源综合考察委员会北京中科院生态环境研究中心北京 100085

The county is recognized as a basic unit for implementation of the strategy for sustainable development. A campaign for construction of eco county has been developed all over China since the 1990s. It is aimed to enhance sustainable development capacity of the county by establishing ecologically sound production system and management mechanism through eco engineering, eco planning and eco management. It is a complicated systems engineering and a combination of multi disciplinary and needs the most extensive participation of decision makers, technicians, and farmers. Construction of eco county consists of many important works such as survey, systems diagnosis, planning, demonstration, implementation and assessment. In this paper, we will try to summarize the current status, main models in different regions, procedure of construction, and introduce the eco agriculture county construction in detail by taking Dazu County of Sichuan Province as an example.

关键词： eco county eco agriculture county sustainable development Dazu County.