Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin
Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin作者机构:College of Chemistry & Chemical Engineering Shanghai Jiaotong University Shanghai 200240 China
出 版 物:《Chinese Chemical Letters》 (中国化学快报(英文版))
年 卷 期:2008年第19卷第6期
页 面:707-710页
核心收录:
学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学]
基 金:This work was financially supported by the National Natural Science Foundation of China (No.20675052, 20727005) National High-Tech R&D Program (No.2006AA03Z324)
主 题:CdTe quantum dots Bovine serum albumin Capillary electrophoresis mobility shift assay Interaction
摘 要:In this work, the capillary electrophoresis mobility shift assay (CEMSA) was first adopted to study the interaction of protein with quantum dots (QDs). In this study, bovine serum albumin (BSA) and CdTe QDs were used as model samples. We observed that BSA was facilely adsorbed to CdTe QDs surface, and the QD-BSA complex was formed by a 1:1 stoichiometric ratio. A value of 2.17 4-0.27 × 10^6 mol^-1 L^-1 (at 25 ℃) for the association constant was obtained by CEMSA.