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Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

Rice science 2024年第1期31卷 62-76页

作者： GAO Ningning YE Shuifeng ZHANG Yu ZHOU liguo MA Xiaosong YU Hanxi li tianfei HAN Jing liU Zaochang LUO lijun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China College of life Sciences Shangrao Normal UniversityShangrao 334001China Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme *** this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of *** under water loss *** expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia *** NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling *** analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related ***,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling *** traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG ***,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT *** two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton ***,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

关键词： antioxidant enzyme β-carotene ketolase drought resistance Nostoc flagelliforme osmotic stress rice transcriptome analysis

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Design Strategies and Practice Paths for Improving Urban Quality in the Stock Era

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Journal of Landscape Research 2024年第2期16卷 18-24页

作者： li tianfei Zhou Xiaotian Han Shengsheng liu Yuxuan Power China Huadong Engineering Corporation limited HangzhouZhejiang 311500China

In the middle and later stages of urbanization development,the growth of the real estate industry will stagnate,and urban renewal will become the *** the advancement of urban renewal,there are still problems in improving the quality of cities in the stock era and their design *** paper analyzed the linping Old City organic renewal project and the Xishui River ecological governance project in the stock era of urban quality improvement by sorting out the current development status,historical background,planning types,and design strategies of quality improvement in the stock era from the perspective of urban renewal,combining with project overview,main problems,design methods,and design *** renewal is the leading direction for promoting urban development and construction on a global scale,and countries formulate different plans and practices based on their local *** renewal strategies should be diversified,and focus on livable environments,urban characteristics,etc.,while considering human factors,green innovation,etc.,in order to achieve smart community management and enhance the economic and social benefits brought by urban *** successful cases such as the linping Old City and Xishui River ecological governance project,corresponding urban quality improvement strategies and implementation plans should be formulated according to local conditions,with emphasis on social participation and people’s livelihood *** study can help urban planning pay more attention to rational utilization and upgrading of existing urban resources,adapt to the current urban development situation,and promote sustainable urban development.

关键词： Stock era Organic renewal Ecological governance linping Old City Xishui River

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海水淹溺性急性肺损伤的致病机制及救治策略研究进展

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华西医学 2024年第1期39卷 147-152页

作者：李田飞张灏魏梁锋王守森亳州市中医院脑外科安徽亳州236800 中国人民解放军联勤保障部队第九〇〇医院神经外科福州350025

海水淹溺导致急性肺组织结构损伤,引起肺通气和换气功能障碍,诱发急性肺水肿甚至急性呼吸衰竭。海水淹溺性急性肺损伤致病机制复杂,涉及炎性反应、肺水肿、肺泡表面活性物质、氧化应激及细胞凋亡和自噬等方面。及时有效的救治,是降低海... 详细信息

海水淹溺导致急性肺组织结构损伤,引起肺通气和换气功能障碍,诱发急性肺水肿甚至急性呼吸衰竭。海水淹溺性急性肺损伤致病机制复杂,涉及炎性反应、肺水肿、肺泡表面活性物质、氧化应激及细胞凋亡和自噬等方面。及时有效的救治,是降低海水淹溺性急性肺损伤患者致死率和致残率的关键。该文对海水淹溺性急性肺损伤的致病机制和救治策略2个方面的研究进展进行归纳总结,旨在为临床工作中海水淹溺性急性肺损伤患者的综合救治和后续的相关研究提供参考。

关键词：海水淹溺肺损伤海水淹溺性急性肺损伤致病机制救治策略

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基于“GEP”增值为导向的浙江山区河流廊道多要素规划方法——以瓯江河川公园为例

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世界建筑导报 2024年第1期39卷 33-37页

作者：李天飞李俊杰屈泽龙刘雨璇李晓中国电建集团华东勘测设计研究院有限公司生态环境工程院浙江省华东生态环境工程研究院

各地积极践行“两山”理念,探索出许多创新举措和应用案例,生态系统生产总值(Gross Ecosystem Product,GEP)核算有助于提高人们主动参与生态系统保护行动、提高人们自然生态保护意识,受到广泛关注。浙江西南山区受制于交通不便、建设用... 详细信息

各地积极践行“两山”理念,探索出许多创新举措和应用案例,生态系统生产总值(Gross Ecosystem Product,GEP)核算有助于提高人们主动参与生态系统保护行动、提高人们自然生态保护意识,受到广泛关注。浙江西南山区受制于交通不便、建设用地局促、生态保护红线的制约,是名副其实的生态高地,经济发展洼地。本次以丽水瓯江河川公园规划为例,以“GEP”增值为导向探讨多要素的规划方法,结合全域水网建设背景和山水单元特点,集合多维要素,统筹考虑防洪防枯、滨水休闲开发、美丽乡村、水环境管理、智慧工程规划、基础设施工程等,为地区发展赋能,推进资源换资产,资产变资本,完善政策体系设计,拓展延伸生态产品价值链。

关键词： GEP 浙江山区河道规划方法多要素

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上海市农业种质资源信息共享服务平台设计与应用

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植物遗传资源学报 2024年第3期25卷 421-430页

作者：王国军卫海滨杨华李天菲林田龙渡刘鸿艳冯芳君石群芳牛婷婷周丽王飞陈亮罗利军龙萍上海市农业生物基因中心上海201106

上海市农业种质资源信息共享服务平台是对上海市保护单位的农业种质资源进行规范管理和共享利用的服务体系。为了解决当前规模化和多样化的种质分散于不同保护单位,不利于资源信息共享与利用的问题,本研究根据农业种质资源库(圃)的工作... 详细信息

上海市农业种质资源信息共享服务平台是对上海市保护单位的农业种质资源进行规范管理和共享利用的服务体系。为了解决当前规模化和多样化的种质分散于不同保护单位,不利于资源信息共享与利用的问题,本研究根据农业种质资源库(圃)的工作流程研发了以保护单位、资源用户和主管单位为主要角色的一站式共享服务平台,为保护单位建立了管理系统模块,保护单位管理员可进行本单位的种质资源数据管理、资源共享信息发布、申请审核和利用成效管理,促进各保护单位种质资源数据的规范化和共享服务。为资源用户创建了申请共享和利用反馈机制,主要提供种质资源信息查询服务、申请利用服务、利用成效反馈服务等,可同时申请多个保护单位的种质。为主管单位提供全面调查的统计汇总信息,包括全市资源存量、资源分发利用、资源利用成效等信息,实时掌握资源共享动向和利用成效。种质资源信息共享服务平台将为保护单位、资源用户和主管单位提供便捷的服务,有助于提高农业种质资源保存与利用效率。

关键词：种质资源保护单位共享服务信息管理

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Endothelial phosphodiesterase 4B inactivation ameliorates endothelial-to-mesenchymal transition and pulmonary hypertension

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1726-1741页

作者： Yanjiang Xing Yangfeng Hou tianfei Fan Ran Gao Xiaohang Feng Bolun li Junling Pang Wenjun Guo Ting Shu Jinqiu li Jie Yang Qilong Mao Ya Luo Xianmei Qi Peiran Yang Chaoyang liang Hongmei Zhao Wenhui Chen Jing Wang Chen Wang State Key Laboratory of Respiratory Health and Multimorbidity Institute of Basic Medical SciencesChinese Academy of Medical SciencesPeking Union Medical CollegeBeijing 100005China Department of Lung Transplantation National Center for Respiratory MedicineNational Clinical Research Center for Respiratory DiseasesChina–Japan Friendship HospitalInstitute of Respiratory MedicineChinese Academy of Medical SciencesBeijing 100029China Haihe Laboratory of Cell Ecosystem Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjin 300051China The State Key Laboratory of Complex Severeand Rare DiseasesInstitute of Basic Medical SciencesChinese Academy of Medical SciencesDepartment of PathophysiologyPeking Union Medical CollegeBeijing 100005China Department of Pharmacy West China HospitalSichuan UniversityChengdu 610044China

Pulmonary hypertension (PH) is a fatal disorder characterized by pulmonary vascular remodeling and obstruction. The phosphodiesterase 4 (PDE4) family hydrolyzes cyclic AMP (cAMP) and is comprised of four subtypes (PDE4A–D). Previous studies have shown the beneficial effects of pan-PDE4 inhibitors in rodent PH;however, this class of drugs is associated with side effects owing to the broad inhibition of all four PDE4 isozymes. Here, we demonstrate that PDE4B is the predominant PDE isozyme in lungs and that it was upregulated in rodent and human PH lung tissues. We also confirmed that PDE4B is mainly expressed in the lung endothelial cells (ECs). Evaluation of PH in Pde4b wild type and knockout mice confirmed that Pde4b is important for the vascular remodeling associated with PH. In vivo EC lineage tracing demonstrated that Pde4b induces PH development by driving endothelial-to-mesenchymal transition (EndMT), and mechanistic studies showed that Pde4b regulates EndMT by antagonizing the cAMP-dependent PKA–CREB–BMPRII axis. Finally, treating PH rats with a PDE4B-specific inhibitor validated that PDE4B inhibition has a significant pharmacological effect in the alleviation of PH. Collectively, our findings indicate a critical role for PDE4B in EndMT and PH, prompting further studies of PDE4B-specific inhibitors as a therapeutic strategy for PH.

关键词： Phosphodiesterase 4B Pulmonary hypertension Endothelial-to-mesenchymal transition

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Blue revolution for food security under carbon neutrality: A case from the water-saving and drought-resistance rice

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Molecular Plant 2022年第9期15卷 1401-1404页

作者： Hui Xia Xianxian Zhang Yi liu Junguo Bi Xiaosong Ma Anning Zhang Hongyan liu liang Chen Sheng Zhou Huan Gao Kai Xu Haibin Wei Guolan liu Feiming Wang Hongyang Zhao Xingxing Luo Danping Hou Qiaojun Lou Fangjun Feng liguo Zhou Shoujun Chen Ming Yan tianfei li Mingshou li LeiWang Zaochang liu Xinqiao Yu HanweiMei lijun Luo Shanghai Collaborative Innovation Center of Agri-Seeds Shanghai Agrobiological Gene CenterShanghaiChina Shanghai Academy of Agricultural Sciences ShanghaiChina Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization Ministry of Agriculture and Rural AffairsShanghaiChina Key Laboratory of Low-carbon Green Agriculture in Southeastern China Ministry of Agriculture and Rural AffairsChina

The increasing concentration of greenhouse gases(GHGs)in Earth's atmosphere leads to global warming,which further causes a series of climate changes and does great harm to both human society and natural *** GHG emissions,mainly in theform of methane(CH4)and nitrous oxide(N2O),areasignificantsourceofGHGs,accountingfor~14%total global GHGs(Zhang et al.,2022).One major source of agricultural GHGs is CH4 emissions from rice paddies,which is responsiblefor~10%-12%ofhuman-inducedCH4emissions(van Groenigen et al.,2013)and contributes~2.40%to the enhanced global warming effect(Zhang et al.,2022).The global warming potential of GHGs emissions from rice systems is roughly four times higher than either wheat or maize(linquist et al.,2012).

关键词： drought neutral resistance

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冻土地区铁路路基主动供热防冻胀方法现场试验研究

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铁道学报 2024年第4期46卷 156-166页

作者：胡田飞张宗凯张晓东何宝华王力石家庄铁道大学省部共建交通工程结构力学行为与系统安全国家重点实验室河北石家庄050043 石家庄铁道大学河北省交通工程结构力学行为演变与控制重点实验室河北石家庄050043 石家庄铁道大学土木工程学院河北石家庄050043 国能新朔准池铁路(山西)有限责任公司山西朔州036002

路基冻胀是冻土地区铁路运营的顽疾,在防排水、土质改良和保温等措施难以消除冻胀的情况下,人工供热是一种备选方案。依托准池铁路K44+970—K45+020冻害路段,设计基于地源热泵的分布式供热方案,建设1个长度为20 m的现场试验段。在2021—... 详细信息

路基冻胀是冻土地区铁路运营的顽疾,在防排水、土质改良和保温等措施难以消除冻胀的情况下,人工供热是一种备选方案。依托准池铁路K44+970—K45+020冻害路段,设计基于地源热泵的分布式供热方案,建设1个长度为20 m的现场试验段。在2021—2022年冬季开展1个冻融周期的供热试验,基于监测数据对热泵换热温度、路基温度场、冻结深度、轨道变形量等指标进行分析。研究结果表明:热泵的供热温度可达50℃以上,热源品位高且供热量稳定。供热试验段内路基冻结范围和温度极值比天然工况显著减小,线路中心处最大冻结深度由148 cm减小为88 cm,冻结锋面保持在地下水毛细迁移高度以上。试验段路基横向冻结深度差值由天然条件的49 cm减小为13 cm,有利于消除横向冻胀差异引起的水平不平顺。试验段纵向上的冻结深度差值基本控制在20 cm以内,可以避免次生高低不平顺。天然路基呈先发育深层冻胀、后在降雪融水入渗时发育浅层冻胀的规律,最大冻胀量达9.4 mm。试验段内路基未发育深层冻胀,且浅层冻胀量得到有效控制,轨道变形量控制在±3 mm以内,没有超出作业验收管理值,有效缓解了试验段冻害问题。

关键词：路基冻胀分布式供热系统地源热泵供热温度温度场冻结深度冻胀量

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Forest Policies Addressing Climate Change in China

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Chinese Forestry Science and Technology 2010年第2期9卷 1-12页

作者： WU Shuirong li Zhiyong YU tianfei Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing 100091, P. R. China

As a developing country with a large population and a fragile ecological environment, China is particularly vulnerable to the adverse effects of climate change. Beginning with the Rio Conference of 1992 China has played a progressively enhanced role in combating climate change. A series of policies and measures to address climate change have been taken in the overall context of national sustainable development strategy, making positive contributions to the mitigation and adaptation to climate change, among which forestry linked policies have been given increasing priority to over the years. At the UN Summit on Climate Change in September 2009, China's President committed the country to an unprecedented increase in forest carbon sink through augmenting forest coverage by 40 million ha and forest stock volume by 1.3 billion m3 by 2020 compared to the 2005 level. For realizing it China has evolved the Forestry Action Plan to Cope with Climate Change that sets forth five basic principles that include combining the targets of forestry development with the national strategies on climate change, increasing forest size and improving forest quality, increasing carbon trading and controlling emissions, combining government guidance with social participation, slowing down climate change, and adapting to the change. Further, in order to make it people's program rather than a top-down government initiative, China is speeding up the trend of decentralization and privatization of forest management through collective forest tenure reform that are geared towards releasing direct governmental control over forest management, decentralizing the powers of forest administrations to lower levels and promoting community participation in forest management. These comprehensive steps have changed the face of forestry in the country but both the government and the scientific community realize that this is merely a beginning in the long and arduous task of combating climate change.

关键词： climate change mitigation adaptation forest policy China

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化学植筋在AP1000核电站后置埋件安装工程中的应用

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施工技术 2013年第21期42卷 29-31页

作者：武伟李天飞国核工程有限公司上海200233

对于有特殊要求的后置埋件,若能避免高温对植筋胶的不利影响,可通过化学植筋直接焊接钢板来实现后置埋件的锚固功能。结合某核电站工程实例,介绍了避免焊接高温不利影响的技术思路和施工工艺。经过理论计算和拉拔试验验证,这种经过特殊... 详细信息

对于有特殊要求的后置埋件,若能避免高温对植筋胶的不利影响,可通过化学植筋直接焊接钢板来实现后置埋件的锚固功能。结合某核电站工程实例,介绍了避免焊接高温不利影响的技术思路和施工工艺。经过理论计算和拉拔试验验证,这种经过特殊工艺施工的化学植筋能满足后置埋件承载力所需要的锚固能力。

关键词：核电站后置埋件化学植筋焊接

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