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Cloning and sequence analysis of Sox genes in a tetraploid cyprinid fish,Tor douronensis

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Chinese Science Bulletin 2008年第13期53卷 1988-1995页

作者： GUO BaoCheng li junbing TONG ChaoBo HE ShunPing Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of the Chinese Academy Sciences Beijing 100049 China

A PCR survey for Sox genes in a young tetraploid fish Tor douronensis (Teleostei:Cyprinidae) was per-formed to access the evolutionary fates of important functional genes after genome duplication caused by polyploidization event. Totally 13 Sox genes were obtained in Tor douronensis,which represent SoxB,SoxC and SoxE groups. Phylogenetic analysis of Sox genes in Tor douronensis provided evidence for fish-specific genome duplication,and suggested that Sox19 might be a teleost specific Sox gene member. Sequence analysis revealed most of the nucleotide substitutions between duplicated copies of Sox genes caused by tetraploidization event or their orthologues in other species are silent substitutions. It would appear that the sequences are under purifying selective pressure,strongly suggesting that they repre-sent functional genes and supporting selection against all null allele at either of two duplicated loci of Sox4a,Sox9a and Sox9b. Surprising variations of the intron length and similarities of two duplicated copies of Sox9a and Sox9b,suggest that Tor douronensis might be an allotetraploidy.

关键词：四倍体短基因基因复制基因多样性

Cyprininae phylogeny revealed independent origins of the Tibetan Plateau endemic polyploid cyprinids and their diversifications related to the Neogene uplift of the plateau

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Science China(life Sciences) 2016年第11期59卷 1149-1165页

作者： Xuzhen Wang Xiaoni Gan junbing li Yiyu Chen Shunping He Key Laboratory of Aquatic Biodiversity and Conservation of the Chinese Academy of Sciences Institute of Hydrobiology Chinese Academy of Sciences

Origin and diversification of the Tibetan polyploid cyprinids(schizothoracins) may help us to explore relationships between diversification of the cyprinids and the Tibetan Plateau uplift. Cyprininae phylogeny was analyzed using mitochondrial and nuclear DNA sequences to trace origins of polyploidy and diversifications of schizothoracins. Ancestral states reconstruction for ploidy levels indicated that the Cyprininae was diploid origin and the schizothoracin clades tetraploid origins. There were two diversification rate shifts along with diversification of the cyprinine fishes in response to the Tibetan uplift. The unusual diversification shifts were located to branches subtending the clades of Tibetan polyploid cyprinids. Our analyses suggested that(i) phylogeny of Cyprininae recovered two independent origins of the Tibetan polyploidy schizothoracins;(ii) diversifications of the schizothoracins were closely related to the Neogene uplift of the Tibetan plateau in the following ways: the relatively ancient Late Oligocene-Middle Miocene adaptive radiation may be associated with the uplift of the southern Tibet and Himalaya; the Middle Miocene-Early Pleistocene lineage-specific diversification broadly coincident with major phase of the Neogene Tibetan uplift; and the most recent Pleistocene diversification shift in Schizothorax closely coincident with the successive Kunlun-Huanghe and Gonghe movements of the Tibetan uplift and the glaciation-induced climate oscillations on the plateau.

关键词： Cyprininae polyploid schizothoracins the Tibetan Plateau diversifications

Cyprinid phylogeny based on Bayesian and maximum likelihood analyses of partitioned data:implications for Cyprinidae systematics

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Science China(life Sciences) 2012年第9期55卷 761-773页

作者： WANG XuZhen GAN XiaoNi li junbing MAYDEN Richard L. HE ShunPing Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Department of Biology Saint Louis UniversitySaint LouisMissouri 63103-2010USA

Cyprinidae is the biggest family of freshwater fish, but the phylogenetic relationships among its higher-level taxa are not yet fully resolved. In this study, we used the nuclear recombination activating gene 2 and the mitochondrial 16S ribosomal RNA and cytochrome b genes to reconstruct cyprinid phylogeny. Our aims were to （i） demonstrate the effects of partitioned phylo- genetic analyses on phylogeny reconstruction of cyprinid fishes; （ii） provide new insights into the phylogeny of cyprinids. Our study indicated that unpartitioned strategy was optimal for our analyses; partitioned analyses did not provide better-resolved or -supported estimates of cyprinid phylogeny. Bayesian analyses support the following relationships among the major mono- phyletic groups within Cyprinidae：（Cyprininae, Labeoninae）, （（Acheilognathinae, （（Leuciscinae, Tincinae）, Gobioninae））, Xenocyprirlinae）. The placement of Danioninae was poorly resolved. Estimates of divergence dates within the family showed that radiation of the major cyprinid groups occurred during the Late Oligocene through the Late Miocene. Our phylogenetic analyses improved our understanding of the evolutionary history of this important fish family.

关键词： Cyprinidae phylogeny molecular dating partitioned analyses

Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei:Cypriniformes)

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Progress in Natural Science:Materials International 2008年第2期18卷 155-160页

作者： Xianghui Kong a,b,Xuzhen Wang a,Xiaoni Gan a,junbing li a,Shunping He a,a Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China b College of life Science,Henan Normal University,Xinxiang 453007,China Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China College of life Science Henan Normal University Xinxiang 453007 China

Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth,differentiation,apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study,19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification,cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ),maximum parsimony (MP),maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages,respectively,with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini,species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel (insertion/deletion) was found at the 50 end of CTGF gene of Cyprinidae,and this 6 bp deletion only appeared in the Leuciscini,which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein,the most variations and indels were distributed in the signal region and IGFBP region of this protein,implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.

关键词： Connective tissue growth factor Sequence variation Molecular evolution Cyprinidae

基于距离量测的主从式AUV协同定位系统观测性研究

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西北工业大学学报 2012年第4期30卷 547-552页

作者：房新鹏严卫生李俊兵西北工业大学航海学院陕西西安710072

研究了基于距离量测的主从式自主水下航行器(AUV)协同定位系统观测性问题。主AUV可以利用装备的高精度导航设备进行精确定位,从AUV装备低精度导航设备,需融合主从AUV之间的距离量测进行水下定位。针对控制输入对协同定位系统可观测性的... 详细信息

研究了基于距离量测的主从式自主水下航行器(AUV)协同定位系统观测性问题。主AUV可以利用装备的高精度导航设备进行精确定位,从AUV装备低精度导航设备,需融合主从AUV之间的距离量测进行水下定位。针对控制输入对协同定位系统可观测性的影响,建立了从AUV在主AUV体坐标系中的运动学模型,并选取主从AUV间的距离值作为观测量,利用基于lie导数的非线性系统观测性秩判据分析了不同控制输入下协同定位系统的观测性。仿真结果表明,对于可观测的协同定位系统,从AUV的位置估计具有良好的收敛性和定位精度。

关键词：自主水下航行器协同定位控制输入观测性分析

基于DSmT证据折扣理论的短波多通道信息融合方法

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通信学报 2024年第3期45卷 29-40页

作者：李俊兵曾孝平李国军叶昌荣重庆大学微电子与通信工程学院重庆400040 重庆邮电大学超视距可信信息传输研究所重庆400065

针对由于短波信道复杂恶劣、通道信息间冲突突出,经典合并方式对短波多通道信息合并效果不佳问题,提出基于DSmT证据折扣理论的短波多通道信息融合方法。以DSmT证据折扣理论作为融合框架,实现对短波多通道信息融合推理判决。首先,归一化O... 详细信息

针对由于短波信道复杂恶劣、通道信息间冲突突出,经典合并方式对短波多通道信息合并效果不佳问题,提出基于DSmT证据折扣理论的短波多通道信息融合方法。以DSmT证据折扣理论作为融合框架,实现对短波多通道信息融合推理判决。首先,归一化OFDM帧的相对包络标准差,并通过相对包络标准差与折扣因子计算得到折扣权重。然后,通过比特软信息提取函数获得比特概率,以比特概率对基本信任分配函数定量赋值来作为证据。最后,以PCR6融合规则对多通道软信息进行融合推理判决。在多种信道条件、多种调制方式下与经典合并方式的对比仿真实验表明,所提方法可以获得明显的误码率增益和误包增益。

关键词：短波信道信息融合证据折扣理论折扣权重比特概率

Phylogenetic relationships of Cyprinidae (Teleostei:Cypriniformes) inferred from the partial S6K1 gene sequences and implication of indel sites in intron 1

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Science China(life Sciences) 2007年第6期50卷 780-788页

作者： KONG XiangHui1,2, WANG XuZhen1, GAN XiaoNi1, li junbing1 & HE ShunPing1 1 Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 2 College of life Science, Henan Normal University, Xinxiang 453007, China Institute of Hydrobiology Chinese Academy of Sciences Wuhan China College of life Science Henan Normal University Xinxiang China

The family Cyprinidae is widely distributed in East Asia,; has the important phylogenetic significance in the fish evolution. In this study, the 5′ end partial sequences (containing exon 1, exon 2; indel 1) of S6K1 gene were obtained from 30 representative species in Cyprinidae; outgroup using PCR amplification; sequencing. The phylogenetic relationships of Cyprinidae were reconstructed with neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML),; Bayesian methods. Myxocyprinus asiaticus (Catostomidae) was assigned to the outgroup taxon. Similar phylogenetic relationships within the family Cyprinidae were achieved with the four analyses. Leuciscini; Barbini were monophyletic lineages respectively with the high nodal supports. Leuciscini comprises Hypophthalmichthyinae, Xenocyprinae, Cultrinae, Gobioninae, Acheilognathinae; East Asian species of Leuciscinae; Danioninae. Monophyly of East Asian clade was supported with high nodal support. Barbini comprises Schizothoracinae, Barbinae, Cyprininae; Labeoninae. The monophyletic lineage consisting of Danio rerio, D. myersi,; Rasbora trilineata was basal in the tree. In addition, the large fragment indels in intron 1 were analyzed to improve the understanding of Cyprinidae relationships. The results showed that the large fragment indels were correlated with the relations among species. Some conserved regions in intron 1 were thought to be involved in the functional regulation. However, no correlation was found between sequence variations; species characteristic size.

关键词： Cyprinidae, S6K1 gene, phylogenetic relationships, intron

基于模型数据双驱动的短波MUF短期预测网络

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通信学报 2023年第12期44卷 99-111页

作者：李俊兵曾囿钧曾孝平李国军白晨曦重庆大学微电子与通信工程学院重庆400040 中国工程物理研究院电子工程研究所四川绵阳621900 重庆邮电大学超视距可信信息传输研究所重庆400065 陆军工程大学通信士官学校重庆400036

针对短波最大可用频率(MUF)经典模型方法预测精度低及机器学习方法训练集数据获取难度大的问题,提出一种模型数据双驱动的双向门控递归单元(BiGRU)网络用于MUF短期预测。模型驱动方面,利用经典MUF预测模型生成的大规模数据集作为模型驱... 详细信息

针对短波最大可用频率(MUF)经典模型方法预测精度低及机器学习方法训练集数据获取难度大的问题,提出一种模型数据双驱动的双向门控递归单元(BiGRU)网络用于MUF短期预测。模型驱动方面,利用经典MUF预测模型生成的大规模数据集作为模型驱动训练集,经过2D CNN和BiGRU网络联合学习后,获得一个初步网络。数据驱动方面,使用小规模的实测数据集对初步网络进行二次训练,得到最终网络CNN-BiGRU-NN。仿真结果表明,所提网络与GRU网络、LSTM网络以及VOACAP模型相比,在日期尺度和时刻尺度上的平均均方根误差(RMSE)均有降低。

关键词：短波通信最大可用频率短期预测模型数据双驱动 CNN-BiGRU-NN

基于BiSAR制导的战斗机最优引导律及导弹最优制导律设计

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西北工业大学学报 2016年第3期34卷 367-373页

作者：黄伟徐建城李俊兵吴华兴西北工业大学电子信息学院陕西西安710072 空军工程大学航空航天工程学院陕西西安710038

针对半主动自寻的合成孔径雷达制导导弹发射后,战斗机、导弹的运动状态如何同时满足成像分辨率和脱靶量要求的问题,提出一种基于逆最优控制方法的战斗机引导律和导弹制导律。将成像分辨率对战斗机及导弹运动状态的双边约束,转化为对战... 详细信息

针对半主动自寻的合成孔径雷达制导导弹发射后,战斗机、导弹的运动状态如何同时满足成像分辨率和脱靶量要求的问题,提出一种基于逆最优控制方法的战斗机引导律和导弹制导律。将成像分辨率对战斗机及导弹运动状态的双边约束,转化为对战斗机目标线角速度的单边约束;将脱靶量转化为对导弹目标线角速度的约束,从而实现导弹运动的自主性;建立含有待定权值系数的性能指标函数,根据所建立的战斗机引导系统和导弹制导系统的动态调节时间确定该系数,逆向得出最优引导律和最优制导律,仿真结果验证了该算法的有效性。

关键词：空对面导弹双平台合成孔径雷达传感器目标制导律方位向分辨率脱靶量运动方程约束优化角速度闭环控制系统状态方程反馈控制控制器动态响应