Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei:Cypriniformes)
Molecular evolution of connective tissue growth factor in Cyprinidae （Teleostei:Cypriniformes）作者机构：Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China College of Life Science Henan Normal University Xinxiang 453007 China
出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))
年 卷 期：2008年第18卷第2期
页 面：155-160页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 071002[理学-动物学]
基 金：National Program on Key Basic Research Projects, (2004CB117402) National Natural Science Foundation of China, NSFC, (30530120) China Postdoctoral Science Foundation, (2005037684)
主 题：Connective tissue growth factor Sequence variation Molecular evolution Cyprinidae
摘 要：Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth,differentiation,apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study,19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification,cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ),maximum parsimony (MP),maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages,respectively,with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini,species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel (insertion/deletion) was found at the 50 end of CTGF gene of Cyprinidae,and this 6 bp deletion only appeared in the Leuciscini,which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein,the most variations and indels were distributed in the signal region and IGFBP region of this protein,implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.
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