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Adenovirus-mediated expression of pig α(1,3) galactosyltransferase reconstructs Gal α(1,3) Gal epitope on the surface of human tumor cells

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cell Research 2001年第2期11卷 116-124页

作者： XING li, guo HONG XIA, JIAN FEI, FANG HUANG, li he guo institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Gal α(1, 3) Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by α(1, 3) galactosyltransferase [α(1, 3) GT] enzyme on the cells of lower mammalian animals such as pigs and mice. Human has no gal epitope due to the inactivation of α(1, 3) GT gene but produces a large amount of antibodies (anti-Gal) which recognize Gal α(1, 3) Gal structures specifically. In this study, a replication deficient recombinant adenoviral vector Ad5sGT containing pig α(1, 3) GT cDNA was constructed and characterized. Adenoviral vector-mediated transfer of pig α(1, 3) GT gene into human tumor cells such as malignant melanoma A375, stomach cancer SGC-7901, and lung cancer SPC-A-1 was reported for the first time. Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis, although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after Ad5sGT transduction. Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I (UEA I) lectins, Vicia villosa agglutinin (VVA), Arachis hypogaea agglutinin (PNA), and Glycine max agglutinin (SBA) to different degrees. In addition, no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay.

关键词： Adenoviral vector galactosyltransferase Gal α(1 3) Gal gene expression human tumor cell.

Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to Kainic acid-induced seizure in transgenic mice

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cell Research 2001年第1期11卷 61-67页

作者： MA YINGHUA, JIA HUA HU, WEN JUAN ZHAO, JIAN FEI, YUN YU, XIAO GANG ZHOU, ZheN TONG MEI, li he guo (institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china) (shanghai Inst institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China shanghai institute of Physiology Chinese Academy of Sciences Shanghai China

γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.

关键词： γ-aminobutyric acid transporter seizure susceptibility kainic acid electroencephalography transgenic mice

GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

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cell Research 2001年第2期11卷 161-163页

作者： JIN XIAO PING, FANG HUANG, NA YANG, BU FENG LU, JIAN FEI, li he guo institute of biochemistry and cell biology, shanghai institutes for biological science, chinese academy of sciences, 320 Yueyang Road, shanghai 200031, china institute of biochemistry and cell biology Shanghai Institutes for Biological Science Chinese Academy of Sciences Shanghai China

GABA transporter 1(GAT1) takes important roles in multiple physiological processes through the uptake and release of GABA, but the regulation of GAT1 gene expression in different tissues is rarely known. To address the question, first, 5’ Rapid amplification of cDNA end (RACE) was used to determine GAT1 transcriptional starting sites in neonatal mouse cerebral cortex and intestine, adult mouse brain and adult rat testis. The products of 5’RACE were confirmed by DNA sequencing. We found that the transcript of GAT1 in neonatal mouse cerebral cortex and adult mouse brain starts at the same site (inside of exon 1), while in mouse intestine, GAT1 starts transcription in intron 1, and in rat testis, the transcript of GAT1 has an additional untranslation exon to the 5’ direction.

关键词： GABA transporter 1(GAT1) transcriptional starting site 5’ RACE tissue spedcific difference DNA sequencing.

Effect of leukemia inhibitory factor on embryonic stem cell differentiation:implications for supporting neuronal differentiation

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Acta Pharmacologica Sinica 2006年第1期27卷 80-90页

作者： Zhao he~(2,3) Jing-jing li~(2,3) Chang-hong ZheN~(3,4) lin-ying FENG~3 Xiao-yan DING~(2,5)2 Laboratory of Molecular and cell biology and Laboratory of Stem cell biology,institute of biochemistry and cell biology:shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china 3 shanghai institute of Materia Medica.chinese academy of sciences.shanghai 201203,china 4 Graduate School of the chinese academy of sciences,shanghai 200031,china. Laboratory of Molecular and cell biology and Laboratory of Stem cell biology Institute of Biochemistry and Cell Biology shanghai institutes for biological sciences Chinese Academy of Sciences Shanghai China shanghai institute of Materia Medica Chinese Academy of Sciences Shanghai China Graduate School of the chinese academy of sciences Shanghai China

Aim:Leukemia inhibitory factor (liF),a pleiotropic cytokine,has been used ex-tensively in the maintenance of mouse embryonic stem cell *** thiscurrent work,we examined the effect of the liF signaling pathway in embryonicstem (ES) cell differentiation to a neural ***:In the presence of liF(1000U/mL),the production of neuronal cells derived from embryoid bodies (EB)was tested under various culture *** of the liF pathway wasexamined with specific *** effects of cell apoptosis and proliferationon neural differentiation were *** cell differentiation into three-germlayers was ***:Under various culture conditions,neuronal differ-entiation was increased in the presence of *** the liF-activated STAT3signaling pathway with specific inhibitors abolished the neuronal differentiationof ES cells,whereas inhibition of the liF-activated MEK signaling pathway im-paired the differentiation of ES cells toward a glial *** suppressed cellapoptosis and promoted cell proliferation during ES cell *** inhib-ited the differentiation of ES cells to both mesoderm and extraembryonic endo-derm fates,but enhanced the determination of neural ***:These results suggest that liF plays a positive role during the differentiation ofES cells into neuronal cells.

关键词： leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies

Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas ligand

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cell Research 2003年第5期13卷 361-367页

作者： JIA HUA HU, JIE JIANG, YING HUA MA, NA YANG, MAO HU ZHANG, MIN WU, JIAN FEI, li he guo 1 Laboratory of Molecular cell biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china 2 College of life science, Zhejiang University, Hangzhou 310027, china 3 shanghai Research Center for Biomodel Organism, shanghai 201203, china Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China College of life science Zhejiang University Hangzhou China Department of Neurology Beth Israel Deaconess Medical Center and Harvard Medical School Boston USA Department of Chemistry Auburn University Auburn USA shanghai Research Center for Biomodel Organism Shanghai China

It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT) mice and transgenic (TG) mice were treated with acute ethanol (20％ v/v) by introperitoneal injection for five times. After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITC-conjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was

关键词： Fas ligand ethanol apoptosis testes transgenic mouse.

Direct transfer of A20 gene into pancreas protected mice from streptozotocin-induced diabetes

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Acta Pharmacologica Sinica 2004年第6期25卷 19-24页

作者： Lu-yang YU, Bo liN, Zhen-lin ZHANG2, li-he guo3 instituteof biochemistryand cell biology, shanghai institutes for biological sciences, chinese academy ofsciences, shanghai 200031 Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, 2 shanghai JiaoTong UniversityAffiliated Sixth People’s Hospital, shanghai 200233, china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 上海 200031 Center for Preventing and Treating Osteoporosis Osteoporosis Research Unit Shanghai JiaoTong University Affiliated Sixth People's Hospital 上海 200233

AIM: To investigate the efficiency of transfer of A20 gene into pancreas against STZ-induced diabetes. METHODS: PVP-plasmid mixture was directly transferred into the pancreatic parenchyma 2 d before STZ injection. The uptake of plasmid pcDNA3-LacZ or pcDNA3-A20 was detected by PCR and the expression of LacZ was confirmed by histological analysis with X-gal. A20 expression in the pancreas of pcDNA3-A20 transgenic mice was measured by RT-PCR and Western blots. Urine amylase, NO generation, and histological examination were examined. RESULTS: Injection of PVP-plasmid mixture directly into the pancreatic parenchyma increased urine amylase concentration 16 h after operation and reversed it to nearly normal 36 h later. On d 33 LacZ expression could be found in spleen, duodenum, and islets. The development of diabetes was prevented by direct A20 gene transferring into the pancreas and A20-mediated protection was correlated with suppression of NO production. The insulitis was ameliorated in A20-treated mice. CONCLUSION: Injection of PVP-plasmid mixture directly into the pancreatic parenchyma led to target gene expression in islets. Direct transfer of A20 gene into the pancreas protected mice from STZ-induced diabetes.

关键词： adenoviridae streptozocin insulin-dependent diabetes mellitus gene therapy nitric oxide glucose amylases reversed transcriptase polymerase chain reaction Western blotting

A20 overexpression under control of mouse osteocalcin promoter in MC3T3-E1 cells inhibited tumor necrosis factor-alpha-induced apoptosis

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Acta Pharmacologica Sinica 2006年第9期27卷 1231-1237页

作者： Yue-juan QIN~2 Zhen-lin ZHANG~(2,4)Lu-yang YU~3 Jin-wei he~2 Ya-nan HOU~3 Tian-jin liU~3 Jia-cai WU~3 Song-hua WU~2 li-he guo~3 2 Center for Preventing and Treating Osteoporosis,Osteoporosis Research Unit,shanghai Sixth People's Hospital,shanghai Jiao Tong University,shanghai 200233,china 3 institute of biochemistry and cell biology,shanghai institutes for biological science,chinese academy of sciences,shanghai 200031,china Center for Preventing and Treating Osteoporosis Osteoporosis Research Unit Shanghai Sixth People's Hospital Shanghai Jiao Tong University Shanghai China institute of biochemistry and cell biology Shanghai Institutes for Biological Science Chinese Academy of Sciences Shanghai China

Aim:To construct an A20 expression vector under the control of mouse osteocalcinpromoter(OC-A20),and investigate osteoblastic MC3T3-EI cell line,whichstably overexpresses A20 protein prevented tumor necrosis factor(TNF)-alpha-induced ***:OC-A20 vector was constructed by fusing a frag-ment of the mouse osteocalcin gene-2 promoter with human A20 *** the mouse MC3T3-E1 cell line,stably transfected by A20,*** expression of A20 mRNA and A20 protein in the cells weredetected by reverse transcription-polymerase chain reaction(RT-PCR)and West-ern blot analysis,*** determine the specificity of A20 expression inosteoblast,the mouse osteoblastic MC3T3-E1 cell line and mouse embryo fibro-blast NIH3T3 cell line were transiently transfected with *** anti-apoptoticrole of A20 in MC3T3-E1 cells was determined by Flow cytometric analysis(FACS),terminal dUTP nick endo-labeling(TUNEL)and DNA gel electrophoresis analysis(DNA Ladder),***:Weak A20 expression was found in MC3T3-E1 cells with the primers of mouse A20.A20 mRNA and A20 protein expressionwere identified in MC3T3-E1 cells transfected with OC-A20 using RT-PCR andWestern blot *** A20 mRNA expression was found in MC3T3-E1 cellafter MC3T3-E1 cells and NII-/3T3 cells were transient transfected with *** obviously occurred in the rate of apoptosis in the OC-A20 group com-pared with the empty vector(pcDNA3)group by FACS(Phe pcDNA group comparedwith OC-A20 group(Pcells andconfirmed that A20 protects osteoblast against TNF-alpha-induced apoptosis.

关键词： A20 osteoblast apoptosis tumor necrosis factor-alpha

Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice

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cell Research 2001年第2期11卷 135-141页

作者： WANG FU DI, WEI BIAN, liNG WEI KONG, FA JI ZHAO, JUN SheNG guo, NAI he JINGI institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, 320 Yue Yang Road, shanghai 200031, china Department of Publ institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Department of Public health Second Military Medical University Shanghai China

Effects of maternal dietary zinc deficiency on prenatal and postnatal brain development were investigated in ICR strain mice. From d 1 of pregnancy (EO) until postnatal d 20 (P20), maternal mice were fed experimental diets that contained 1 mg Zn/kg/day (severe zinc deficient, SZD), 5 mg Zn/kg/day (marginal zinc deficient, MZD), 30 mg Zn/kg/day (zinc adequately supplied, ZA) or 100 mg Zn/kg/day (zinc supplemented, ZS and pair-fed, PF). Brains of offspring from these dietary groups were examined at various developmental stages for expression of nestin, an intermediate filament protein found in neural stem cells and young neurons. Immunocytochemistry showed nestin expression in neural tube 10. 5 d post citrus (dpc) as well as in the cerebral cortex and neural tube from 10.5 dpc to postnatal d 10 (P10). Nestin immunoreactivities in both brain and neural tube of those zinc-supplemented control groups (ZA, ZS, PF) were stronger than those in zinc-deficient groups (SZD and MZD). Western blot analysis confirmed that nestin levels in -pooled brain extracts from each of the zinc-supplemented groups (ZA, ZS, PF) were much higher than those from the zinc-deficient groups (SZD and MZD) from 10.5 dpc to P10. Immunostaining and Western blots showed no detectable nestin in any of the experimental and control group brains after P20. These observations of an association between maternal zinc deficiency and decreased nestin protein levels in brains of offspring suggest that zinc deficiency suppresses development of neural stem cells, an effect which may lead to neuroanatomical and behavioral abnormalities in adults.

关键词： Zinc deficiency nestin brain development neural stem cells.

Identification of glutamate transporters and receptors in mouse testis

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Acta Pharmacologica Sinica 2004年第3期25卷 112-117页

作者： Jia-hua HU,Na YANG2,Ying-hua MA,Jie JIANG,Jin-fu ZHANG3,Jian FEI4,li-he guo4 Laboratory of Molecular cell biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031 3Department of Urology,Tongji Hospital,Tongji University,shanghai 200065,china 中科院上海生命科学院 Laboratory of Molecular Cell Biology CAS 上海 200031 Department of Urology Tongji Hospital Tongji University 上海 200065

AIM: To investigate the presence of glutamate transporters and receptors in mouse testis. METHODS: Glutamate uptake analysis was performed to study the function of glutamate transporters in mouse testis. Comparative RT-PCR technique and sequencing analysis were used to study the expression of glutamate receptors and transport- ers in mouse testis. RESULTS: Mouse testis possessed glutamate uptake capacity with sodium-dependence. Vmax value of glutamate uptake was (1.60 ± 0.21) pmol/min per mg protein and Km value of glutamate uptake was (11.0±1.6) μmol/L in mouse testis according to saturation analysis. Furthermore, the uptake activity could be inhibited by DHK (GLT1 selective inhibitor) and THA (glutamate uptake inhibitor). In addition, RT-PCR results revealed that glutamate transporters (GLT1 and EAAC1) and ionotropic glutamate receptors (NR1, NR2B, GluR6 and KA2) were expressed in mouse testis. CONCLUSION: Glutamate transporters and receptors do exist in mouse testis.

关键词： glutamates receptors amino acid transport systems testis uptake