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KAI1 is a potential target for anti-metastasis in pancreatic cancer cells

World Journal of Gastroenterology 2008年第7期14卷 1126-1132页

作者： Jian-Hua Xu Xiao-Zhong Guo li-Nan Ren li-chun Shao min-Pei liu Department of Gastroenterology The General Hospital of Shenyang Military Command Shenyang 110016 Liaoning Province China

AIM: To investigate whether KAI1, as a metastasis suppressor gene, is associated with invasive and metastatic ability of pancreatic cancer ***: KAI1 gene was transfected into pancreatic cancer cell line MiaPaCa Ⅱ by liposomes selected with G418. Expression of transfected cells was measured by Western blotting, immunofluorescence and immunocytochemistry. Tumor cell invasion and metastatic ability were detected through gelatinase activity and reconstituted basement membrane (Matrigel) assay. pCMV-KAI1 was directly injected into the heterotopic human pancreatic adenocarcinoma successfully established in the groin of BALB/C nude mice, by subcutaneous injection of MiaPaCa Ⅱ pancreatic cancer cells. The statistical analysis between groups was determined by Student's two tailed t ***: By Western blotting, MiaPaCa Ⅱ cells transfected by KAI1 gene indicated KAI1 expression at approximately 29.1 kDa. Cytoplasm staining was positive and uniformly spread in transfected cancer cells, using immunohistochemistry and immunofluorescence. The most obvious difference was present after 30 h (MiaPaca Ⅱ 43.6 ± 9.42, pCMV-MiaPaca Ⅱ 44.8 ± 8.56, pCMV-KAI1-MiaPaca Ⅱ 22.0 ± 4.69, P lipofectamine 1.22 ± 0.31 in A group were lower than control 4.61 ± 1.98 and pCMV-KAI 11.67 ± 0.81. Analyses of metastases with and without KAI1 transfection in mice were different in liver and lung between controls 1.62 ± 0.39, 0.45 ± 0.09, pCMV-KAI 1.01 ± 0.27, 0.33 ± 0.09 and KAI1-lipofectamine 0.99 ± 0.21, 0.30 ± 0.09 respectively (P lity. KAI1 gene plays an important role in inhibiting metastasis of

关键词： KAI1 Pancreatic cancer cell line Transfection Immunocytochemistry Western blotting Immunofluorescence Gelatinolysis

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Alcohols＇ Classification by Infrared Spectra Segment Based on Support Vector Machines

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Chinese Chemical Letters 2006年第7期17卷 929-932页

作者： Wei XIE Fu Sheng NIE Meng Long li Guang ming li min chun LU College of Chemistry Sichuan University Chengdu 610064

This paper studies various classifiers to identify primary, secondary or tertiary alcohols by using segmental spectra and their combinations to support vector machines （SVMs）. The results showed that the O-H in-plane bending absorption contributed most to identification their substitute. This conclusion disagrees with related known research results.

关键词： Infrared spectra alcohols support vector machine information extraction.

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Some improvements of the marching cubes algorithm for the rendering of an orebody

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Journal of China University of mining and Technology 2008年第2期18卷 194-198页

作者： li chun-min li Zhong-xue WANG Yun-hai HAO Xiu-qiang Institute of mine Safety China Academy of Safety Science and Technology Beijing 100029 China School of Civil and Environment Engineering University of Science and Technology Beijing Beijing 100083 China

Orebody-rendering techniques are developed using the marching cubes (MC) algorithm. The shape of an orebody is viv- idly displayed in real time and can be used to guide mining design as well. The MC algorithm has been improved in two aspects. By analyzing the principles of the MC algorithm, as well as the features of the specific application, improvements were developed to: eliminate ambiguities by using a unified isosurface constructing method in the voxels, and improve the operating efficiency of the MC algorithm by incorporating an octree structure. The analytical results of the examples demonstrate the effectiveness of our proposal.

关键词： underground mines marching cubes algorithm 3D visualization octree

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Inheritance and Molecular Marker of Resistance to Bot Canker in Malus domestica

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Agricultural Sciences in China 2011年第2期10卷 175-184页

作者： liU Hai-tao li Cai-li ZHANG Yu-jing li chun-min ZHAO Yong-bo CHEN Dong-mei WANG Yi ZHANG Xin-zhong HAN Zhen-hai Institute for Horticultural Plants College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China Changli Institute for Pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China College of Horticulture Northeast Agricultural UniversityHarbin 150030P.R.China

Apple bot canker [Botryosphaeria dothidea（Moug.） *** de Not.] is distributed worldwide,resulting in a serious crop loss every year in apple（Malus domestica Borkh.） *** resistance of each seedling derived from a hybrid population（Jonathan×Golden Delicious） was evaluated by disease index either from natural infection in the field or from inoculation with five isolates of ***,Ls1,Lw023,Lw048,Mx1,and *** inheritance of the resistance to bot canker was analyzed via frequency distribution analysis,and microsatellite and AFLP markers linked to the resistance loci were *** the binary frequency distribution patterns,it was found that the segregation ratio of resistant/susceptible genotypes infected by pathogen isolates Lw023 and Ls1 was 1：15;and that by Zz26 and Mx1 was 15：*** variation of resistance was involved in the segregation of two to four alleles of major genes,the resistance was recessive when infected by Lw023 and Ls1,but was dominant when infected with Mx1 and Zz26.A microsatellite maker,CH02a04-450,and two AFLP markers,E-AG/M-GAC-280 and E-AGG/M-CTT-110,were identified,and their map distances to the resistance loci were 5.1,5.1 and 6.2 cM,*** three markers are located in different linkage groups,while CH02a04-450 is on linkage group 2 or 7.E-AG/M-GAC-280 was successfully converted into ***,CH02a04-450 and SCAR159 were re-examined in inoculated segregation population and presented a good reliability on predicting phenotypes of resistance.

关键词： Malus domestica Borkh. Botryosphaeria dothidea inheritance microsatellite AFLP

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Structural and Antileukemic Studies ofIndirubin Derivatives

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Chemical Research in Chinese Universities 1995年第1期11卷 75-78页

作者： TIAN Fa-an li chun-min and li Dai-yu( Deparrment of Chmistry, Deparsment of Medicine ,Luzhou Medical College, Luzhou, 646000)GUO Yun-hong ZHOU Zong-hua and MAO Zhi-hua(Deparrment of Chemistry , Sichuan uniuersity, Chengdu, 610064 ) LUZHOU MED COLL DEPT CHEMLUZHOU 646000PEOPLES R CHINA SICHUAN UNIV DEPT CHEMCHENGDU 610064PEOPLES R CHINA LUZHOU MED COLL DEPT MEDLUZHOU 646000PEOPLES R CHINA

ＳｔｒｕｃｔｕｒａｌａｎｄＡｎｔｉｌｅｕｋｅｍｉｃＳｔｕｄｉｅｓｏｆＩｎｄｉｒｕｂｉｎＤｅｒｉｖａｔｉｖｅｓＴＩＡＮＦａ－ａｎ，ＬＩＣｈｕｎ－ｍｉｎａｎｄＬＩＤａｉ－ｙｕ（ＤｅｐａｒｒｍｅｎｔｏｆＣｈｍｉｓｔｒｙ，Ｄ... 详细信息

ＳｔｒｕｃｔｕｒａｌａｎｄＡｎｔｉｌｅｕｋｅｍｉｃＳｔｕｄｉｅｓｏｆＩｎｄｉｒｕｂｉｎＤｅｒｉｖａｔｉｖｅｓＴＩＡＮＦａ－ａｎ，ＬＩＣｈｕｎ－ｍｉｎａｎｄＬＩＤａｉ－ｙｕ（ＤｅｐａｒｒｍｅｎｔｏｆＣｈｍｉｓｔｒｙ，ＤｅｐａｒｓｍｅｎｔｏｆＭｅｄｉｃｉ...

关键词： Indirubin derivatives Leukemia/activity Structure

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Gender differences are associated with the clinical features of systemic lupus erythematosus

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Chinese Medical Journal 2012年第14期125卷 2477-2481页

作者： DING Yan HE Jing GUO Jian-ping DAI Yi-jun li chun FENG min li Ru li Zhan-guo Department of Rheumatology & Immunology Peking UniversityPeople's Hospital Beijing 100044 China

Background Systemic lupus erythematosus （SLE） mostly occurred in young women. This study was undertaken to investigate the different clinical characteristics of SLE between male and female patients, and to identify the sex hormone levels and clinical outcomes of different gender in SLE patients. Methods Of the 516 SLE patients admitted to the Peking University People＇s Hospital from January 2008 to December 2010, 58 were male and 458 were female. Clinical manifestations, laboratory profiles and disease activity scores were evaluated in male and female patients. Sex hormones levels were also compared among male patients. Results The median age at SLE onset in male and female patients was 27.2 and 28.6 years, respectively. Compared with female patients, at onset of SLE, male patients showed higher rates of serious renal disease （58.6% vs. 47.2%, P=0.064）, neuropsychiatric SLE （20.7% vs. 12.0%, P=0.055）, and a higher incidence of anti-ds-DNA （25.9% vs. 16.8%, P=0.069）, anti-Sm （17.2% vs. 8.7%, P=0.002）, anti-Ro （46.6% vs. 28.4%, P=0.004）, anti-UIRNP （29.3% vs. 15.3%, P=0.010）, anticardiolipin antibody （25.9% vs. 11.4%, P=0.004）, and decreased C3 levels （67.2% vs. 49.8%, P=-0.009）. Systemic lupus erythematosus disease activity index （SLEDAI） scores were higher in men than in women （16.8 vs. 12.8, P=0.038）. Of the 58 male patients, 24 had not received aggressive treatment during the three months prior to the study. Levels of testosterone and dihydroepiandrosterone （DHEA） were lower in male SLE patients than in male healthy controls （P=0.004 and P=0.006, respectively）. Low serum testosterone was an independent risk factor for the development of lupus nephritis （P=-0.043）. Male patients with elevated serum prolactin were at increased risk of developing neuropsychiatric manifestations of SLE （P=0.081）. Conclusion Early recognition of risk factors and appropriate intervention are essential, which might lead to high disease activity and serious systemic damage in male SLE pat

关键词： systemic lupus erythematosus male lupus, gender

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Oxidation of n-Butanol and 2-Pentanol with Molecular Oxygen in Supercritical CO2

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Chinese Chemical Letters 2003年第10期14卷 1070-1072页

作者： Yan Hong CHANG, Tao JIANG, liang GAO, Quo Ying ZHAO, Hai Xiang GAO, Zhong Hao li, Jun chun li, Zhi min liu, Bu Xing HANCenter for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences,Beijing 100080 Center for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

Oxidation of n-butanol and 2-pentanol using molecular oxygen in supercritical (SC) CO2 with and without co-solvent is investigated. The results showed that the reaction selectivity is high when the reaction is carried out in SC CO2. It has been observed that co-solvent affects conversion and selectivity of the reaction considerably.

关键词： Oxidation,n-butanol 2-pentanol supercritical CO2 co-solvent.

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资本积累的全球化与空间的生产

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教学与研究 2010年第6期 32-37页

作者：李春敏同济大学马克思主义学院上海200092

资本积累具有空间性,这缘于资本具有不断征服和占有空间的内在属性,资本的全球化就是资本空间扩张的必然结果。资本的全球化对全球社会空间产生了深远影响,它使全球社会空间呈现出同质性和断裂性的双重特征,"同质性"建构了资本在全球空... 详细信息

资本积累具有空间性,这缘于资本具有不断征服和占有空间的内在属性,资本的全球化就是资本空间扩张的必然结果。资本的全球化对全球社会空间产生了深远影响,它使全球社会空间呈现出同质性和断裂性的双重特征,"同质性"建构了资本在全球空间的权力场域,而"断裂性"则导致了对落后民族和国家的空间剥夺。在马克思、恩格斯以后,列宁、布哈林和卢森堡对帝国主义时代资本积累的空间性进行了再思考,而这些均证明马克思主义的理论体系中并不缺失空间的维度。

关键词：资本空间资本积累全球化

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论马克思空间分析的现象学之维--兼论马克思与空间现象学的对话

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河北学刊 2022年第1期42卷 46-54页

作者：李春敏同济大学马克思主义学院上海200092

马克思的空间分析呈现出自觉的现象学意识,具体表现在:一是对于身体与空间的现象学描述。空间不是作为身体之外的被动的容器,而是人的身体参与其中的差异化场域,人是以身体为中介进行感性活动的,人的身体的丰富性通向感性的丰富性。二... 详细信息

马克思的空间分析呈现出自觉的现象学意识,具体表现在:一是对于身体与空间的现象学描述。空间不是作为身体之外的被动的容器,而是人的身体参与其中的差异化场域,人是以身体为中介进行感性活动的,人的身体的丰富性通向感性的丰富性。二是对客观空间的现象学批判。马克思将空间阐释为一种社会历史的产物,空间的发生学本身就是历史的发生学,空间不只是物理学意义上的实体空间,更是具有主体向度的目的—意志空间、文化—想象空间、一种情感—体验空间。三是关于显现和隐藏的辩证法。空间的直观经验只是马克思空间分析的起点,马克思真正要做的就是从空间的非反思的显现方式,进入到空间的本质的显现方式,消解空间直观经验所给予的不透明的外观,从而使它隐藏的部分得以显现。四是资本权力与差异化空间的释放。资本开启了一个普遍的同质化的空间生产的时代,重建差异化的空间就是扬弃空间生产的资本逻辑,恢复人与空间多维的感性关系。马克思与空间现象学的对话只能限定在方法论以及由这个方法论指引的实践旨趣上,在具体的致思逻辑和实践路径上,马克思与空间现象学的对话呈现出本质性的困境,这体现在关于空间意识的发生学场域的不同理解和关于空间批判的实践归宿的差异。

关键词：马克思空间现象学

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论马克思空间辩证法的四重向度

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河北学刊 2024年第5期44卷 77-84页

作者：李春敏同济大学马克思主义学院

马克思开启了独特的空间辩证法的视野,这一空间辩证法主要关涉四重向度:一是“在场”与“缺场”的空间辩证法,其基本指向是人的对象化活动,人的对象化活动既是在物的世界中人从“缺场”走向“在场”的过程,亦是在人的世界中物从“缺场... 详细信息

马克思开启了独特的空间辩证法的视野,这一空间辩证法主要关涉四重向度:一是“在场”与“缺场”的空间辩证法,其基本指向是人的对象化活动,人的对象化活动既是在物的世界中人从“缺场”走向“在场”的过程,亦是在人的世界中物从“缺场”走向“在场”的过程。在资本主义条件下,“在场”与“缺场”的空间辩证法现实地体现为物的世界的“强势在场”与人的世界的“空前缺场”。二是“同一”与“差异”的空间辩证法,其基本指向是历史空间的总体性与多样性,马克思的历史哲学阐释了现实历史运动的总体的结构性特征,同时,马克思视野中的历史不是无空间差异的均质的线性历史。在资本主义条件下,“同一”与“差异”的空间辩证法具体体现为空间生产的普遍物化与差异化的空间生产的式微。三是“部分”与“整体”的空间辩证法,具体关涉三个维度:现代视野中的民族与世界、个人与社会、经济生活与作为整体的社会生活。四是“中心”与“边缘”的空间辩证法,它在不同的历史阶段中具有不同的形态,在资本主义条件下,“中心”与“边缘”的空间辩证法主要关涉资本宰制下空间生产的不平衡性,这种不平衡性的场域涉及全球空间、城市与乡村之间、城市内部。通过以上四重向度的探讨,马克思既呈现了空间辩证法的“一般”,即一般意义上空间的生产与社会历史过程的辩证关系,同时又呈现了空间辩证法的“特殊”,即资本主义条件下资本积累与空间生产的辩证关系。其中,后者是马克思关注的重点领域。

关键词：马克思空间辩证法四重向度

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