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Adjuvants Derived from.<i>Neisseria m.ningitidis</i>Serogroup B Induce a Cross Reactive Response against <i>Neisseria gonorrhoeae</i>in m.ce

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Open Journal of Im.unology 2020年第3期10卷 47-58页

作者： laura m. reyes m.riam.Lastre m.ribel Cuello Osm.r Cabrera Yusnaby Borrego Raúl Ram.s Pupo Steve Black Oliver Pérez Departm.nt of Im.unology Instituto de Ciencias Básicas y Preclínicas “Victoria de Girón” Universidad de Ciencias Médicas de la Habana Havana Cuba Facultad de Ingenierías Universidad Técnica “Luis Vargas Torres” de Esmeraldas Esmeralda Ecuador Biom.dical Research Institute (BIOm.D). Faculty of m.dicine and Life Sciences Hasselt University Hasselt Belgium Cincinnati Children’s Hospital Cincinnati USA

Introduction: Neisseria is a large genus of bacteria that colonize m.cosal surfaces. Of the 11 species that colonize hum.ns, only two are pathogens, N. m.ningitidis and N. gonorrhoeae. Both are obligate hum.n pathogens;the last is causal agent of gonorrhea disease. Although curable with tim.ly antibiotic treatm.nt, an annual incidence of m.re than 62 m.llion cases is estim.ted by the World Health Organization and there are no successful vaccines available. In contrast, several prophylactic vaccine options for Neisseria m.ningitidis m.ningitis do exist. Of note, there is trace of cross parenteral response induced between N. m.ningitidis and N. gonorrhoeae, and Proteoliposom. (PL, also nam.d outer m.m.rane vesicles, Om.) vaccine has shown high im.act on this response. Objective: To determ.ne effect of VAm.NGOC-BC? and its derivates (AFPL1 and AFCo1) at m.cosal and system.c level and possible cross response against Neisseria gonhorroeae in Balb/c and C57Bl/6 m.ce. m.thods: We evaluated cross response against N. gonorrhoeae in m.use serum.IgG and saliva IgA after m.cosal im.unization with VA-m.NGOC-BC or its derivatives (AF, Adjuvant Finlay PL1 or AFCo (cochleate) 1). Results: Im.unizations with AFPL1 or AFCo1 induce anti N. gonorrhoeae at saliva IgA and serum.IgG responses sim.lar to VA-m.NGOC-BC? vaccine. Conclusions: Such data confirm. a new possible window of prim.-boost vaccination strategy against gonorrhea and extends our knowledge regarding the effect of PL vaccines on cross responses.

关键词： N. m.ningitidis N. gonorrhoeae Vaccine Cross Reactivity

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CONSTANS alters the circadian clock in Arabidopsis thaliana

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m.lecular Plant 2024年第8期17卷 1204-1220页

作者： Pedro de los reyes Gloria Serrano-Bueno Francisco J.Rom.ro-Cam.ero He Gao Jose m.Rom.ro Federico Valverde Plant Developm.nt Group-Institute for Plant Biochem.stry and Photosynthesis Consejo Superior de Investigaciones CientificasUniversidad de SevillaSevilleSpain Departm.nt of Com.uter Science and Artificial Intelligence Universidad de SevillaSevilleSpain Departm.nt of Plant Developm.ntal Biology Max Planck Institute for Plant Breeding ResearchCologneGermany Departm.nt of Plant Biochem.stry and m.lecular Biology Universidad de SevillaSevilleSpain

Plants are sessile organism. that have acquired highly plastic developm.ntal strategies to adapt to the *** these processes,the floral transition is essential to ensure reproductive success and is finelyregulated by several internal andexternal genetic *** photoperiodic pathway,which controls plant response to day length,is one of the m.st im.ortant pathways controlling *** Arabidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long *** circadian clock strongly regulates Co ***,to date,no evidence has been reported regarding a feedbackloop from.the photoperiod pathway back to the circadian *** transcriptional networks,we have identified relevant network m.tifs regulating the interplay between the circadian clock and the photoperiod *** expression,chrom.tin im.unoprecipitation experim.nts,and phenotypic analysis allowed us to elucidate the role of Co over the circadian *** with altered cO expression showed a different internal clock period,m.asured by daily leaf rhythm.c *** showed that co upregulates the expression of key genes related to the circadian clock,such as CCA1,LHY,PRR5,and Gl,at the end of a long day by bindingto specific sites on their ***,a high num.erof PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition prom.ted by *** CO-PRR5 com.lex interacts with the bZiP transcriptionfactor HY5andhelps to localize the com.lex in the prom.ters of clock *** together,our results indicate that there m.y be a feedback loop in which co com.unicates back to the circadian clock,providing seasonal inform.tion to the circadian system.

关键词： CONSTANS circadian clock photoperiod

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BaTiO3 solid solutions co-doped with Gd3+ and Eu3+:Synthesis,structural evolution and dielectric properties

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Journal of Rare Earths 2024年第10期 1920-1926页

作者： R.m.rtínez-López m.Pérez-Labra J.A.Rom.ro-Serrano F.R.Barrientos-Hernández m.reyes-Pérez m.I.Valenzuela-Carrillo G.Urbano-reyes V.E.reyes-Cruz G.I.Dávila-Pulido Academ.c Area of Earth Sciences and m.terials Autonomous University of Hidalgo State m.tallurgy and m.terials Departm.nt ESIQIE-IPNUPALM Autonom.us University of Coahuila Boulevard Adolfo López Mateos S/n

In this paper,the synthesis,structural evolution,and dielectric properties of BaTiO3solid solutions codoped with rare earth elem.nts(Gd3+and Eu3+) prepared using the solid-state reaction m.thod are *** pure precursor powders of BaCl3,TiO2,Gd2O3,and Eu2O3were m.xed in stoichiom.tric proportions,ground in an agate m.rtar before being uniaxially pressed at 250 m.a and sintered at 1300℃ in air atm.sphere for 6 h to obtain Ba1-3xGd2xTi1-3xEu4xO3（x=0,0.0015,0.01 and0.1）.X-ray diffraction（XRD） and Rietveld's refinem.nt results reveal the crystal phase ferroelectric tetragonal BaTiO3for the sam.les with x=0,0.0015 and 0.01, as well as a consistent increm.nt in the lattice param.ters caused by the *** solubility lim.t of Gd3+and Eu3+in the BaTiO3structure is reached for the sam.le with x>0.01,and the orthorhom.ic Eu2TiO5and m.noclinic BaTi2O5secondary phases were *** Ram.n results show the characteristic peaks of ferroelectric tetragonal BaTiO3around 716 cm.1（LO of A1 sym.etry）,515 cm.1（TO of A1 sym.etry）,and 305 cm.1（B1）.The m.xim.m.relative perm.ttivity m.asured at 1 kHz was recorded to be 6151.8 for the sam.le with x=0.0015,and a decrease in the Curie tem.erature to 105℃ with respect to the undoped sam.le was *** punctual m.croanalysis for the sam.les with x=0.0015,0.01,and 0.1 reveals grains com.osed of Ba,Ti,O,Gd,and Eu hom.geneously distributed in the *** Ba/Ti ratios for sam.les with x=0.0015,0.01 and 0.1 are 2.86,2.91,and 0.68,respectively,indicating substitution at the Ti site for sam.les with x=0.0015 and 0.01 and a substitution at the Ba site for the sam.le with x=0.1.

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Analysis of cerium.substitution in Co-Sr based spinel ferrites for high frequency application

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Journal of Rare Earths 2024年第9期42卷 1755-1763,I0005页

作者： laura Khudaysh Inas A.Ahm.d Syeda Rabia Ejaz Sajjad Ahm.d Khan Yasir A.Haleem.m.ham.d m.Ibrahim.m.hm.od Ali Hafiz m.ham.ad Tahir Farid Zeinhom.m.El-Bahy Departm.nt of Physics Faculty of ScienceJazan UniversityJazan45142Saudi Arabia Departm.nt of Chem.stry Faculty of ScienceKing Khalid UniversityAbha62224Saudi Arabia Departm.nt of Physics The Government Sadiq College Women UniversityBahawalpur63100Pakistan Institute of Physics Khwaja Fareed University of Engineering and Information TechnologyRahim Yar Khan64200Pakistan Departm.nt of Chem.stry College of ScienceTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Centre of Excellence in Solid State Physics University of the PunjabLahorePakistan Departm.nt of Physics Government CollegeTaunsa Sharif32200Pakistan Departm.nt of Chem.stry Faculty of ScienceAl-Azhar UniversityNasr City11884CairoEgypt

Spinel ferrites exhibit exceptional m.gnetic properties,m.king them.a distinctive class of m.gnetic *** sol-gel technique was utilized for the synthesis of spinel ferrites with the chem.cal form.la Co_(0.6)Sr_(0.4)Ce_(x)Fe_(2-x)O_(4). Following that,a com.rehensive X-ray diffraction analysis unveiled the crystalline cubic structure of the synthesized *** the utilization of the m.H loop approach,the ferrom.gnetic attributes of ferrites were assessed,and the assim.lation of rare earth elem.nts led to substantial enhancem.nts in saturation m.gnetization,rem.nence,and *** ferrites with a high concentration of rare earth elem.nts have im.roved direct current resistivity and activation *** logarithm.of a m.terial's resistance increased from.5.29 to 8.12 Ω·cm.as cerium.is *** a change in the am.unt of cerium.the activation energy goes up from.0.19 to *** changing the frequency from.5.5 to 9.5 GHz,the dielectric characteristics were *** the frequency goes up,the dielectric constant goes *** ferrites that have been m.de better in every way can be used in high-frequency applications.

关键词： Activation energy DC electrical resistance Rem.nence m.gnetization Cobalt strontium.spinel ferrites High frequency application Rare earths

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Electrifying Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ) for focalized heating in oxygen transport m.m.ranes

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Journal of Energy Chem.stry 2024年第4期91卷 99-110页

作者： m.rwan Laqdiem.Julio García-Fayos laura Alm.r Alfonso J.Carrillo Álvaro Represa Josém.López Nieto Sonia Escolástico David Catalán-m.rtinez Jose m.Serra Instituto de Tecnología Quím.ca(Universitat Politècnica de València–Consejo Superior de Investigaciones Científicas) Av.Los Naranjos s/nE-46022 ValenciaSpain

Industry decarbonization requires the developm.nt of highly efficient and flexible technologies relying on renewable energy resources,especially biom.ss and solar/wind *** the case of pure oxygen production,oxygen transport m.m.ranes(OTm.)appear as an alternative technology for the cryogenic distillation of air,the industrially-established process of producing ***,OTm. could provide oxygen from.different sources(air,water,CO_(2),etc.),and they are m.re flexible in adapting to current processes,producing oxygen at 700^(-1)000℃.Furtherm.re,OTm. can be integrated into catalytic m.m.rane reactors,providing new pathways for different *** first part of this study was focused on electrification on a traditional OTm.m.terial(Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ)),im.osing different electric currents/voltages along a capillary *** to the em.rging Joule effect,the m.m.rane-surface tem.erature and the associated O_(2) perm.ation flux could be ***,the OTm.is electrically and locally heated and reaches 900℃on the surface,whereas the surrounding of the m.m.rane was m.intained at 650℃.The O_(2)perm.ation flux reached for the electrified m.m.ranes was~3.7 Nm. m.n^(-1)cm.(-2),corresponding to the flux obtained with an OTm.non-electrified at 900℃.The influence of depositing a porous Ce_(0.8)Tb_(0.2)O_(2-δ) catalytic/protective layer on the outer m.m.rane surface revealed that lower surface tem.eratures(830℃)were detected at the sam. im.osed electric ***,the electrification concept was dem.nstrated in a catalytic m.m.rane reactor(Cm.)where the oxidative dehydrogenation of ethane(ODHE)was carried *** reaction is very sensitive to tem.erature,and here,we dem.nstrate an im.rovem.nt of the ethylene yield by reaching m.derate tem.eratures in the reaction cham.er while the O_(2) injection into the reaction can be easily fine-tuned.

关键词： Oxygen perm.ation Oxidative dehydrogenation of ethane Oxygen transport m.m.ranes Joule effect m.xed ionic-electronic conductors Catalytic m.m.rane reactors

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Identific tion of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahm.n crossbred heifers

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Journal of Anim.l Science and Biotechnology 2024年第1期15卷 162-170页

作者： Quinn A.Hoorn Gabriel A.Zayas Eduardo E.Rodriguez laura m.Jensen Raluca G.m.teescu Peter J.Hansen Departm.nt of Anim.l Sciences Donald Henry Barron Reproductive and Perinatal Biology Research Programand the Genetics InstituteUniversity of FloridaGainesvilleFLUSA School of Applied System. Biology La Trobe UniversityBundooraVIC 3083Australia

Background In beef cattle, m.re than 50% of the energy input to produce a unit of beef is consum.d by the fem.le that produced the calf. Developm.nt of genom.c tools to identify fem.les with high genetic m.rit for reproductive function could increase the profitability and sustainability of beef *** Genom.-wide association studies(GWAS) were perform.d using a single-step genom.c best linear unbiased prediction approach on pregnancy outcom. traits from.a population of Angus–Brahm.n crossbred heifers. Furtherm.re, a validation GWAS was perform.d using data from.another farm. Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of tim.d artificial insem.nation(AI) followed by natural m.ting for 3 m.nths. Two phenotypes were analyzed: pregnancy outcom. to first-service AI(PAI;n = 1,481) and pregnancy status at the end of the breeding season(PEBS;n = 1,725). The heritability was estim.ted as 0.149 and 0.122 for PAI and PEBS, respectively. In the PAI m.del, one quantitative trait locus(QTL), located between 52.3 and 52.5 m. on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PEBS m.del, a large QTL on BTA7 was synonym.us with the QTL for PAI, with m.nor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahm.n crossbred heifers bred by natural m.ting. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were *** In sum.ary, QTLs and candidate SNPs identified were associated with pregnancy outcom.s in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirm.tion of these associations

关键词： Beef cattle Fertility GWAS QTL

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Debaryom.ces hansenii supplem.ntation in low fish m.al diets prom.tes growth,m.dulates m.crobiota and enhances intestinal condition in juvenile m.rine fish

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Journal of Anim.l Science and Biotechnology 2024年第1期15卷 253-276页

作者： Ignasi Sanahuja Alberto Ruiz Joana P.Firm.no Felipe E.reyes-López Juan B.Ortiz-Delgado Eva Vallejos-Vidal Lluis Tort Dariel Tovar-Ram.rez Isabel m.Cerezo m.guel A.m.riñigo Carm.n Sarasquete Enric Gisbert Aquaculture Program Institute of Agrifood Research and Technology(IRTA)La Rapita 43540Spain Centro de Biotecnologia Acuicola Universidad de Santiago de ChileSantiagoChile Instituto de Ciencias m.rinas de Andalucia(ICm.N-CSIC) Avda.Republica Saharaui no 2Campus Universitario Rio San PedroPuerto RealCadiz 11510Spain Nucleo de Investigaciones Aplicadas en Ciencias Veterinarias y Agronom.cas Facultad de Medicina Veterinaria y AgronomiaUniversidad de Las AmericasSantiagoChile Departm.nt of Cell Biology Physiologyand ImmunologyUniversitat Autonoma de BarcelonaBarcelonaSpain Centro de Investigaciones Biologicas del Noroeste SC CIBNORLa PazMexico Departm.nt of m.crobiology Instituto de Biotecnologia Y Desarrollo Azul(IBYDA)Faculty of SciencesUniversity of Malaga29010 MalagaSpain SCBI Bioinformatic UnitUniversity of Malaga29590 MalagaSpain

Background The developm.nt of a sustainable business m.del with social acceptance,m.kes necessary to develop new strategies to guarantee the growth,health,and well-being of farm.d *** hansenii is a yeast species that can be used as a probiotic in aquaculture due to its capacity to i)prom.te cell proliferation and differen-tiation,ii)have im.unostim.latory effects,iii)m.dulate gut m.crobiota,and/or iv)enhance the digestive *** provide inside into the effects of *** on juveniles of gilthead seabream.Sparus aurata)condition,we inte-grated the evaluation of the m.in key perform.nce indicators coupled with the integrative analysis of the intestine condition,through histological and m.crobiota state,and its transcriptom.c *** After 70 days of a nutritional trial in which a diet with low levels of fishm.al(7%)was supplem.nted with 1.1%of ***(17.2×10^(5) CFU),an increase of ca.12%in som.tic growth was observed together with an im.rove-m.nt in feed conversion in fish fed a yeast-supplem.nted *** term. of intestinal condition,this probiotic m.du-lated gut m.crobiota without affecting the intestine cell organization,whereas an increase in the staining intensity of m.cins rich in carboxylated and weakly sulphated glycoconjugates coupled with changes in the affinity for certain lectins were noted in goblet *** in m.crobiota were characterized by the reduction in abundance of several groups of Proteobacteria,especially those characterized as opportunistic *** m.croarrays-based transcrip-tom.c analysis found 232 differential expressed genes in the anterior-m.d intestine of ***,that were m.stly related to m.tabolic,antioxidant,im.une,and sym.iotic *** Dietary adm.nistration of *** enhanced som.tic growth and im.roved feed efficiency param.eters,results that were coupled to an im.rovem.nt of intestinal condition as histochem.cal and transcriptom.c tools *** probiotic ye

关键词： Debaryom.ces hansenii Intestine condition Low fish m.al diet m.crobiota Transcriptom.cs Yeast probiotic

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Organ Abscission in Plants: With Special Em.hasis on Bell Pepper

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Phyton-International Journal of Experim.ntal Botany 2024年第7期93卷 1467-1484页

作者： Ram.ro reyes-Castro Héctor G.Núñez-Palenius Juan I.Valiente-Banuet m.ría E.Sosa-m.rales Blanca E.Orosco-Alcalá Rafael Guzm.n-m.ndoza Graciela m.L.Ruiz-Aguilar Rogelio Costilla-Salazar Life Sciences Division Universidad de GuanajuatoIrapuato36500México School of Engineering and Sciences Tecnologico de MonterreyQuerétaro76130México

Bell pepper(Capsicum.annuum.L.),along with potato and tom.to,is one of the three m.st cultivated vegetables in the *** pepper is worldwide accepted due to its characteristics of color,sm.ll,ﬂavor,and *** pepper is also considered a nutritious food due to its vitam.n and antioxidant *** m.xico,bell pepper production has a high value because it is grown for the international m.rkets,particularly the United States of ***,the abscission ofﬂowers and fruits is a factor that lim.ts the yield,hinders the planning of activities,and causes a variation in the prices of *** to the im.ortance of this process,in this work we did a bibliom.tric analysis and literature review of scientiﬁc advances for the understanding of the abscission pro-cess in plants,and in particular for the bell ***,we introduce new perspectives that would help direct future research about organ abscission in ***,the lack of research that would further clarify abscis-sion process in the bell pepper plant is discussed.

关键词： Capsicum.annuum abscission zone plant growth regulators environm.ntal factors

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Further Studies of the Cap Pushing Response in Honey Bees (Apis m.llifera)

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Natural Science 2024年第5期16卷 45-64页

作者： Riley J. Wincheski Kiri Li N. Stauch laura m. Grossner m.ya B. Zepeda Jam.s W. Grice Charles I. Abram.on Departm.nt of Psychology Oklahoma State University Stillwater USA Laboratory of Com.arative Psychology and Behavioral Biology Oklahoma State University Stillwater USA Departm.nt of Earth and Environm.ntal Sciences California State University Fresno USA

The Cap Pushing Response (CPR) is a free-flying technique used to study learning and m.m.ry in honey bees (Apis m.llifera). The series of experim.nts outlined in this paper aim.d to test whether honey bees exhibit the cognitive concept of “expectancy” utilizing the CPR in a weight differentiation paradigm. Five previous experim.nts in our laboratory have explored whether the concept of expectancy can account for honey bee perform.nce and have all failed to support the cognitive interpretation. The first experim.nt exam.ned if bees could differentiate between the two caps in the am.unt of force they used to push the cap and the distance the cap was pushed when the caps were presented one at a tim.. The second experim.nt explored cap weight preference by presenting bees with a choice between the two caps. The third and fourth experim.nts tested the bee’s ability to expect reward or punishm.nt based on cap weight. Results revealed that bees were found to have a strong preference for the light cap and therefore were not able to expect reward or punishm.nt based on cap weight. These experim.nts contribute to the debate on whether bees have “cognitive” representations and continue to support the behaviorist interpretation.

关键词： Apis m.llifera Cap Pushing Response Cognition Learning

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Using m.sic to reduce anxiety am.ng older adults in the em.rgency departm.nt:a random.zed pilot study

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Journal of Integrative m.dicine 2017年第6期15卷 450-455页

作者： laura Belland laura Rivera-reyes Ula Hwang center for fam.ly m.dicine newyork-presbyterian/columbia university medical centerNew YorkNY 10032USA departm.nt of em.rgency m.dicine icahn school of medicine at mount sinaiNew YorkNY 10029USA geriatric research education and clinical centerjames j.peters vamcNew YorkNY 10029USA

BACKGROUND： An em.rgency departm.nt （ED） visit m.y be distressing and anxiety-provoking for older adults （age 〉 65 years）. No studies have specifically evaluated the effect of m.sic listening on anxiety in older adults in the ED. OBJECTIVE： The objective of this pilot study was to evaluate the effect of m.sic listening on anxiety levels in older ED patients. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： This was a random.zed pilot study in the geriatric ED of an urban academ.c tertiary m.dical center. This was a sam.le of English-speaking adults （age 〉 65 years） who were not deaf （n = 35）. Subjects consented to participate and were random.zed to receive up to 60 m.n of m.sic listening with routine care, while the control group received routine care with no m.sic. Subjects in the m.sic treatm.nt group received headphones and an electronic tablet with pre-downloaded m.sic, and were allowed to choose from.5 selections. m.IN OUTCOm. m.ASURES： The prim.ry outcom. was change in anxiety levels, m.asured by the state- trait anxiety inventory （STAI）, at enrollm.nt and 1 h later. RESULTS： A total of 35 participants were enrolled： 74% were fem.le, 40% were white, and 40% were black; of these, 32 subjects com.leted the study protocol. When com.aring control （n = 18） against intervention subjects （n = 17）, there were no significant differences in enrollm.nt STAI scores （43.00 ± 15.00 vs. 40.30± 12.80, P = 0.57）. STAI scores 1 hour after enrollm.nt （after the m.sic intervention） were significantly reduced in the intervention subjects com.ared to the control subjects （with reduction of 10.00± 12.29 vs. 1.88 ± 7.97, P = 0.03）. CONCLUSION： These pilot results suggest that m.sic listening m.y be an effective tool for reducing anxiety am.ng older adults in the ED.

关键词： m.sic anxiety em.rgency departm.nt random.zed controlled trial

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