检索结果-南通市图书馆

中国植物病理学会2023年学术年会

作者： WU Yupo FU Tongyu HOU Jiachang liu Jie HUANG Junyan liu Lijiang liu shengyi The Key Laboratory of Biology and Genetic Improvement of Oil Crops the Ministry of Agriculture and Rural Affairs of the PRC Oil Crops Research Institute Chinese Academy of Agricultural Sciences

Sclerotinia stem rot(SSR),a destructive disease caused by the fungus Sclerotinia sclerotiorum,is a major factor limiting the yield and quality of oilseed rape in the *** SSR resistance in *** is quantitative and(controlled by a set of minor *** of these genes and pyramiding them into a variety are a major strategy for SSR resistance breeding in ***,a genomewide association study(GWAS) using a natural population of *** consisting of 222 accessions are identify BnaA08 g25340 D(BnML02) as a candidate gene that regulates the SSR *** expressed BnMLO2_ 2 in Arabidopsis and the transgenic plants displayed an enhanced SSR *** profiling of different tissues of *** revealed that BnML02 had the most expression level in leaf and silique tissues among all the 7 BnMLO2 members and also expressed higher in the SSR resistant accession than in the susceptible *** of ML02 conferred plants an enhanced SSR resistance whereas mlo2 plants displayed reduced resistance to SSR in *** regulation of MLO2 in SSR resistance may be associated with cell *** and phylogenetic;analysis revealed a large expansion of MLO family in Brassica *** study revealed an important role of BnMLO2 in the regulation of SSR resistance and provided a new gene candidate for future improvement of SSR resistance in *** and also new insights into understanding MLO family evolution in Brassica crops.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

BnaOmics:A comprehensive platform combining pan-genome and multi-omics data from Brassica napus

引用

Plant Communications 2023年第5期4卷 86-89页

作者： Xiaobo Cui Ming Hu Shengli Yao Yuanyuan Zhang Minqiang Tang Lijiang liu Xiaohui Cheng Chaobo Tong shengyi liu Key Laboratory of Biology and Genetic Improvement of Oil Crops The Ministry of Agriculture and Rural AffairsOil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants(Ministry of Education) College of ForestryHainan UniversityHaikou 570228China

Dear Editor,Brassica napus was originally formed7500 years ago by the interspecific hybridization of *** and ***(Chalhoub et al.,2014).It accounts for approximately 13%–16%of global vegetable oil production and provides an excellent model for polyploid genomics and evolutionary research in ***,the Brassicaceae Database(BRAD V3.0;Chen et al.,2022),Genoscope(https://***/brassicanapus/),and Ensembl Plants(https://***)are used for genomic research pertaining to ***,they only provide genome browsers for research into the *** cultivar *** *** pan-genome information resource(BnPIR)also provides eight *** reference genomes(Song et al.,2021),gene information,and resequencing *** *** variation information resource(BnVIR)provides information on genetic variation,including single-nucleotide polymorphisms(SNPs),insertions or deletions(INDELs),and structural variations(SVs)(Yang et al.,2022).

关键词： comprehensive insertion resource

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The early flowering time gene AtRDR mediated resistance to Sclerotiniastem rot in Arabidopsis thaliana

The early flowering time gene AtRDR mediated resistance to S...

引用

2018全国植物生物学大会

作者： Zuo Rong Zhu Wei Zetao Bai Rongfang Zhou Caihua Dong shengyi liu The Key Laboratory of Biology and Genetic Improvement of Oil Crops The Ministry of Agriculture of PRCOil Crops Research InstituteChinese Academy of Agriculture Sciences

Canola(Brassica napus) is a very important oil crop as a major source of edible oil in the world and plays an important role in China national *** rot(Sclerotiniasclerotiorum) is a major disease on canola,and has become a major limiting factor on rapeseed *** of rapeseed genes related to disease resistance and cultivation of disease-resistant varieties are current urgent *** this research,a *** gene was selected for functional study and its Arabidopsis ortholog AtRDR producing two splicing variants of AtRDRl and AtRDRl,was genetically proved controlling early flowering in *** analyses revealed that the lines of over-expression of AtRDRl and AtRDR2 were more sensitive to the Sclerotinia disease,but the knockout mutant rdrwas more *** analyzing the expression level of PR1,PDF1.2 andAtrbohB,marker genes for SA,JA and ROS signaling pathways,respectively,we found that AtRDRl and AtRDR2 over-expressing plants were significantly higher in expression of AtPRl expression than the knock-out mutant and WT;AtRbohB exhibited a similar trend in these lines;In contrast,AtPDF1.2 expression in the mutant was significantly higher than that in over-expression plants and *** suggested that AtRDR controls resistance through negative regulation of the JA pathway and positive regulation of the SA *** study found that Arabidopsis early flowering gene AtRDR negative regulates Sclerotinia disease,provides an important evidence for resistance to Sclerotiniain relation to flowering time.

关键词： AtRDR Brassica napus Arabidopsis thaliana resistance to Sclerotiniastem rot

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

QTL mapping and Gene Identification of Branch number in Brassica napusL.

QTL mapping and Gene Identification of Branch number in Bras...

引用

2018全国植物生物学大会

作者： Zhixue Dong Chuanji Zhao Khorshed Alam Muhammad Junyan Huang Yueying liu Xiaohui Cheng shengyi liu The Key Laboratory of Biology and Genetic Improvement of Oil Crops The Ministry of Agriculture of PRCOil Crops Research InstituteChinese Academy of Agricultural Sciences

Branching is an important trait for the genetic improvement of rapeseed(Brassica napus L.).However,the genetic mechanism of branch number is not well *** the present study,a recombinant inbred line(RIL) population derived from a cross between few-and many-branches lines was identified for the phenotype in different environment and genotyped with whole genome 60 K SNP array.A major quantitative trait locus(QTL) for the branch number was mapped to a 2.48-Mb region on chromosome A02,explaining 28.12%of phenotype variance at ***,we constructeda near inbred line(NIL) population(BC4 F2) and developed polymorphic molecular markers according to the re-sequencing data of two parental lines for fine *** we have investigated the phenotype of more than 7000 individuals and next step we’ll search for the recombinant events with polymorphic markers,which can be narrow down the QTL interval *** studies are in progress and I think it is valuable for gene cloning and molecular breeding of branching in rapeseed.

关键词： Brassica napus branch number RIL NIL QTL mapping

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Co-expression of antimicrobial peptide BnPRP1 and RsAFP2 in transgenic Arabidopsis thaliana confers enhanced resistance to Sclerotnia sclerotiorum

Co-expression of antimicrobial peptide BnPRP1 and RsAFP2 in ...

引用

中国植物病理学会2019年学术年会

作者： Li Yan Bai Zetao Huang Junyan Zuo Rong He Yizhou Cheng Xiaohui liu Yueying liu shengyi Oil Crops Research Institute of Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetics Improvement of Oil CropsMinistry of Agriculture and Rural Affairs

Brassica napus（oilseed rape） is an economically important oil crop all over the *** not only provides vegetable oil for people,but also provides high quality fodder for *** addition,owing to its favorable agronomic properties,such as cultivation under different seasons and rotation with cereals,*** is preferred by farmers ***,Sclerotinia stem rot（SSR） is a devastating fungal disease and a major yield limiting factor in oilseed rape *** is caused by Sclerotinia sclerotiorum（Lib.） de Bary,a cosmopolitan pathogen of many economically important *** a necrotrophic pathogen,it infects more than 600 plant species,including important oil crops such as oilseed rape,soybean,and *** not only deteriorates the quality of the seed,but also significantly reduces the oil *** develop resistant materials against this fungal disease,the antimicrobial peptide gene BnPRP1 of *** and RsAFP2 from Raphanus sativus were expressed in Arabidopsis thaliana with a linker *** integration and expression of transgenes in TO and T2 generation plants were confirmed by PCR and qRT-PCR *** crude proteins showed significant inhibition of *** hyphal *** homozygous T2 plants,showing inheritance in Mendelian fashion（3:1）,were further evaluated under greenhouse conditions for resistance to *** vital leaf inoculation,restricted size and expansion of lesions were observed in transgenic plants compared with wild ***,co-expression of BnPRP1 and RsAFP2 in Arabidopsis thaliana provide subsequent protection against SSR disease and can be implemented in transgenic ***.

关键词： Brassica napus Sclerotinia sclerotiorum antimicrobial peptide resistance

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Mapping loci controlling fatty acid profiles and contents of oil and protein by genome-wide association study in Brassica napus

Mapping loci controlling fatty acid profiles and contents of...

引用

2018全国植物生物学大会

作者： Minqiang Tang Yuanyuan Zhang Yueying liu Chaobo Tong Xiaohui Cheng Wei Zhu Junyan Huang shengyi liu The Key Laboratory of Biology and Genetic Improvement of Oil Crops The Ministry of Agriculture of PRCOil Crops Research InstituteChinese Academy of Agriculture Sciences

Optimal profile and quantity of fatty acids in rapeseed are very critical for maximizing thevalue of edible oil and ***-wide association study(GWAS) could efficiently identify the genes and their network underlying complex traits and provide molecular markers to accelerate *** this study,weperformed GWAS todissect the genetic variations of eightseed quality traits in a rapeseed population comprising370 diverse *** common SNPs for the contents of seed oiland proteinwere detected on five chromosomes,and five genes orthologous to functionknown Arabidopsis genes were predicted as candidate *** region of reduced variation aroundFAE1 loci onA08 and C03 chromosomes controlling the content of erucic acid showed that these two regions were strongly selected during *** the re-sequencing data,we found,except the known haplotypes combination of the variations of ***1 and ***,three accessions contained an additional haplotype combination controlling the moderate content of erucic *** study reveals the genetic variations and complex correlations of eight quality traits,which may inform breeding selection strategies for traits in crops.

关键词： association study fatty acid profiles candidate genes Brassica napus

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Metabolite-Based Genome-Wide Association Study to Identify Resistance-Related Genes Against Sclerotinia Sclerotiorum in Brassica napus

Metabolite-Based Genome-Wide Association Study to Identify R...

引用

2018全国植物生物学大会

作者： Yuanyuan Zhang Minqiang Tang Yizhou He Junyan Huang Yueyingliu Xiaohui Cheng Jie liu Lijiang liu Chaobo Tong shengyi liu The Key Laboratory of Biology and Genetic Improvement of Oil Crops The Ministry of Agriculture of PRCOil Crops Research InstituteChinese Academy of Agriculture Sciences

Brassica napus is a worldwide important oil *** stem rot(SSR) caused by the fungal pathogen Sclerotiniasclerotiorum is one of the most devastating diseases worldwide and seriously affects the yield and quality of *** resistance to *** in *** a quantitative trait and controlled by multiple quantitative trait loci(QTLs).But all the QTLs identified for *** resistance only explained a small portion of the phenotypic variation,and it is still difficult to clone the candidate genes in these ***-based genome-wide association study(mGWAS) has displayed supplementary advantage in identification of functional genes and in understanding plant disease resistance-related metabolic ***,we collected 800 accessions of *** from all over the world andgenotypedthen by *** phenotype data of resistance to SSR and thevariations of metabolites from leaves inoculated with and without thepathogen were collected and analyzed by GWAS and mGWAS,respectively.25 common candidate genes highlighted and mapped to defense metabolites pathway were identified,and the function of them was verified by molecular biology *** results reveal a picture of plant metabolites and underlying genes related todisease SSR resistance,and will promote efficient utilization of metabolites in improvementof disease resistance breeding.

关键词： Brassica napus metabolite profiling resistanceto Sclerotiniasclerotiorum GWAS mGWAS

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The Draft Genome of Brassica oleracea and Evolution of Brassica Genomes

The Draft Genome of Brassica oleracea and Evolution of Brass...

引用

第十二届全国植物基因组学大会

作者： shengyi liu Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan,Hubei 430062,China

Brassica napus,*** and *** are three important crops *** originated from hybridization between *** and *** and the consequent *** of genomes and their evolution of these three species is of significance for genetic improvement of these *** the purpose, we sequenced the genomes of *** and ***(coordinated by Institute of Vegetable and Flower of CAAS) and are sequencing the genome of *** this report,we present comparative analysis in the levels of structure and genes between the genomes of *** and *** which are possibly further

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

BnLPT1 is a causal gene underlying a quantitative locus conferring resistance to Sclerotnia sclerotiorum

BnLPT1 is a causal gene underlying a quantitative locus conf...

引用

中国植物病理学会2019年学术年会

作者： Zuo Rong Tang Minqiang Bai Zetao Li Yan Huang Junyan Zhong Xue He Yizhou Cheng Xiaohui liu Yueying liu shengyi Oil Crops Research Institute of Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetics Improvement of Oil CropsMinistry of Agriculture and Rural Affairs

Brassica napus（oilseed rape） is one of the four major oil crops in the world and is the first source of China domestic edible *** stem rot（SSR）,caused by Sclerotinia sclerotiorum,is reported to the most serious diseases in oilseed *** current agricultural production,the measures for controlling SSR mainly include chemical and biological control and field management,etc.,which have certain effect,but the effect is not only relatively limited but also increases the production cost,and leads to environmental ***,selection and breeding disease-resistant varieties is the most economic and effective way to control *** this study,a significant QTL,located on A04 chromosome,was identified by analyzing recombinant population and associated population combined with the phenotype data under natural field conditions and indoors inoculation which were related to SSR resistance,such as SSR incidence and disease *** homologous gene in Arabidopsis thaliana is AtLTP1（At2 g38540）.Therefore,we over-expressed BnLTP1 of *** and AtLTP1 in *** wild type,at the same time,RNAI interferences of AtLTPl was *** got stable integration and expression of transgenes in TO and T2 generation plants through PCR and qRT-PCR *** resistance to *** of transgenic materials,which show inheritance in Mendelian fashion（3:1）,were evaluated under greenhouse *** results revealed that the lines of over-expression of BnLTP1 and AtLTPl were more resistant to ***,but the interference lines of AtLTPl were more sensitive to *** at 36 h after inoculation and ***,the crude proteins of BnLTP1 and AtLTP1 expressed in prokaryotes could significantly inhibit the hyphal growth of *** through in vitro bacteriostasis *** BnLTP1 can improve resistance to SSR in oilseed rape.

关键词： Brassica napus Sclerotinia sclerotiorum resistance BnLTP1

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Identification of Male sterility-related Genes in Brassica napus toward a novel design for both pollination control system and disease control

Identification of Male sterility-related Genes in Brassica n...

引用

湖北省遗传学会第八次代表大会暨学术讨论会

作者： Wang Zhuoyu,Dong Caihua,liu shengyi~* Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan,430062, China

Male sterility is an inheritable trait characterized by the inability of a plant to produce functional *** main types of male sterility in plant were discovered:cytoplasmic male sterility(CMS) and genic male sterility(GMS).GMS is a type of male sterility controlled by nuclear *** male sterility genes may play roles in different development stages of microspore *** on the expression profile analysis of Brassica napus male sterility material Shaan-GMS using Arabidopsis thaliana ATH-1 microarray,some greatly diversified expressing genes were selected to identify and investigate the effect of those genes on genetic male *** this purpose,artificial microRNA vectors were used to interfere expression of these genes in *** target sequences of each gene were designed for microRNA *** vectors were transferred into Arabidopsis by a method of flower *** transgenic Arabidopsis was screened with 5ppm *** phenotypes of transgenic offspring were observed during microspore *** and cellular methods were used to further identify functions of Ms-related *** methods included PCR for screening out the transgenic Arabidopsis,Q-PCR for validating microRNA vector’s interfering efficiency, Arekisanda staining for identification of the extent of microspore *** them,some genes have been identified involving in pollen *** positively transgenic plants had no pollen during the flowing period and showed microspore abortion by Alexander staining.Q-PCR validated that expression levels of the gene in these transgenic lines are about 1-5%of the wild *** results showed that interfering by artificial microRNA leads to post-transcriptional gene silencing,which caused male *** characterization of the gene can contribute to the understanding of the molecular mechanisms of fertility control and stamen development in oilseed *** these results, we have developed a wor

关键词： DGMS artificial microRNA realtime PCR

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：