Co-expression of antimicrobial peptide BnPRP1 and RsAFP2 in transgenic Arabidopsis thaliana confers enhanced resistance to Sclerotnia sclerotiorum
作者单位：Oil Crops Research Institute of Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetics Improvement of Oil CropsMinistry of Agriculture and Rural Affairs
会议名称：《中国植物病理学会2019年学术年会》
会议日期：2019年
学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治]
关 键 词：Brassica napus Sclerotinia sclerotiorum antimicrobial peptide resistance
摘 要：Brassica napus（oilseed rape） is an economically important oil crop all over the *** not only provides vegetable oil for people,but also provides high quality fodder for *** addition,owing to its favorable agronomic properties,such as cultivation under different seasons and rotation with cereals,*** is preferred by farmers ***,Sclerotinia stem rot（SSR） is a devastating fungal disease and a major yield limiting factor in oilseed rape *** is caused by Sclerotinia sclerotiorum（Lib.） de Bary,a cosmopolitan pathogen of many economically important *** a necrotrophic pathogen,it infects more than 600 plant species,including important oil crops such as oilseed rape,soybean,and *** not only deteriorates the quality of the seed,but also significantly reduces the oil *** develop resistant materials against this fungal disease,the antimicrobial peptide gene BnPRP1 of *** and RsAFP2 from Raphanus sativus were expressed in Arabidopsis thaliana with a linker *** integration and expression of transgenes in TO and T2 generation plants were confirmed by PCR and qRT-PCR *** crude proteins showed significant inhibition of *** hyphal *** homozygous T2 plants,showing inheritance in Mendelian fashion（3:1）,were further evaluated under greenhouse conditions for resistance to *** vital leaf inoculation,restricted size and expansion of lesions were observed in transgenic plants compared with wild ***,co-expression of BnPRP1 and RsAFP2 in Arabidopsis thaliana provide subsequent protection against SSR disease and can be implemented in transgenic ***.