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Biomedical and Environmental Sciences 2019年第12期32卷 881-892页

作者： CHE Jie LU Jin Xing li wen ge ZHANG Yun Fei ZHAO Xiao Fei YUAN Min BAI Xue Mei CHEN Xia li Juan State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention

Objective Antimicrobial resistance(AMR)has become a global concern and is especially severe in *** effectively and reliably provide AMR data,we developed a new high-throughput real-time PCR assay based on microfluidic dynamic technology,and screened multiple AMR genes in broiler fecal *** A high-throughput real-time PCR system with an new designed integrated fluidic circuit assay were performed AMR gene detection.A total of 273 broiler fecal samples collected from two geographically separated farms were screened AMR *** The new assay with limits of detection ranging from 40.9 to 8,000 copies/*** sensitivity rate,specificity rate,positive predictive value,negative predictive value and correct indices were 99.30%,98.08%,95.31%,99.79%,and 0.9755,*** this assay,we demonstrate that AMR genes are widely spread,with positive detection rates ranging from 0 to 97.07%in 273 broiler fecal *** CTX-M,bla TEM,mcr-1,fex A,cfr,optr A,and int I1 showed over 80%*** dissemination of AMR genes was distinct between the two *** We successfully established a new high-throughput real-time PCR assay applicable to AMR gene surveillance from fecal *** widespread existence of AMR genes detected in broiler farms highlights the current and severe problem of AMR.

关键词： Antimicrobial resistance Antimicrobial resistance gene High-throughput real-time PCR array Broiler fecal sample

Single‑cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes

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Journal of Animal Science and Biotechnology 2024年第2期15卷 614-631页

作者： Ting ge Yifan wen Bo li Xiaoyu Huang Shaohua Jiang Enping Zhang Key Laboratory of Animal genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China

Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of *** the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific *** sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy *** the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×genomics single-cell RNA *** A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each ***,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa ***,four granulosa cell subtypes were *** analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa ***,the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly *** genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular *** This study provides insights into the molecular mechanisms underlying the high fecundity of Hu *** differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female *** findings also highlight the importance of genes such as JPH1,LOC101112291,FTH1,and FTL in regulating granulosa cell function and follicular development.

关键词： Granulosa cells Hu sheep Lambing number Ovarian somatic cells Single-cell RNA sequencing

Ultrahigh strength and uniform corrosion in Zn-li alloys through ultrahigh pressure supersaturated solid solution treatment for biodegradable orthopedic applications

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Rare Metals 2024年第10期43卷 5284-5304页

作者： Wan-Ying li Yi-Long Dai wen-Hao Cai Si-Han lin lin Guo De-Chuang Zhang Yuncang li Cuie wen School of Materials Science and Engineering Xiangtan University Department of Prosthodontics Shanghai Ninth People′s HospitalShanghai Jiao Tong University Key Laboratory of Low Dimensional Materials&Application Technology Ministry of EducationXiangtan University School of Engineering RMIT UniversityMelbourne

Zn-li alloys are considered promising candidate materials for biodegradable orthopedic implant applications due to their high mechanical performance and good ***,the presence of a large number of second-phase particles in this class of Zn alloys can lead to severe localized degradation due to micro-galvanic corrosion,which is detrimental to the mechanical integrity of the alloys during tissue healing in the human *** this study we report ultrahigh strength,uniform corrosion,good cytocompatibility,and effective antibacterial ability in Zn-x li（x=0.3,0.5,0.7;wt%） alloys achieved through supersaturated solid solution treatment （SSST） at 375℃ under ultrahigh pressure of 3 GPa.A high concentration of β-liZn4phases coexisted with the-Zn matrix in the as-cast Zn-x li alloys,whereas almost all the li dissolved into the-Zn matrix of SSST *** yield strength was 437 MPa for SSST Zn-0.3li,592 MPa for SSST Zn-0.5 li and 686 MPa for SSST *** SSST Zn-li alloys showed uniform degradation with remarkably reduced degradation rates compared to their as-cast *** 25% concentration extracts of the Zn-x li alloys demonstrated no cytotoxicity toward MC3T3-E1 cells,and the alloys exhibited effective antibacterial ability agains methicillin-resistant staphylococcus aureus.

关键词： Corrosion behavior Mechanical property Supersaturated solid solution treatment (SSST) Ultrahigh pressure Zn-li alloy

Layered niobium carbide enabling excellent kinetics and cycling stability of li-Mg-B-H hydrogen storage material Layered niobium carbide enabling excellent kinetics

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Rare Metals 2024年第3期43卷 1153-1166页

作者： li-wen Lu Hui Luo Guang-Xu li Yun li Xin-Hua Wang Cun-Ke Huang Zhi-QiangLan wen-Zheng Zhou Jin Guo Mohammad Ismail Hai-Zhen liu Guangxi Novel Battery Materials Research Center of Engineering Technology Guangxi Colleges and Universities Key Laboratory of Blue Energy and Systems IntegrationSchool of Physical Science and TechnologyGuangxi UniversityNanning530004China State Key Laboratory of Featured Metal Materials and life-Cycle Safety for Composite Structures Nanning530004China School of Mechanical and Electrical Engineering Quzhou College of TechnologyQuzhou324000China Department of Materials Science and Engineering Zhejiang UniversityHangzhou310027China Faculty of Ocean Engineering Technology and Informatics Universiti Malaysia Terengganu21030Kuala NerusTerengganuMalaysia Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai UniversityTianjin300071China

The li-Mg-B-H composite(2liBH_(4)+MgH_(2))has a high capacity of 11.4 wt%as a hydrogen storage ***,the slow kinetics and poor cycling stability severely restrict its practical *** this work,a layered Nb_(2)C MXene was first synthesized and then introduced to tailor the kinetics and cycling stability of the li-Mg-B-H *** milled 2liH+MgB_(2)composites were initially hydrogenated to obtain the 2liBH_(4)+MgH_(2)*** 2liBH_(4)+MgH_(2)+5wt%Nb_(2)C can release 9.0 wt%H_(2)in 30 min at 400℃,while it is only 2.7 wt%for the undoped 2liBH_(4)+MgH_(2).The dehydrogenation activation energies of MgH_(2)and liBH_(4)are 123 and 154 kJ·mol^(-1)respectively for the 5 wt%Nb_(2)C-doped composite,lower than the undoped composite(164 and 165 kJ·mol^(-1)).The 2liBH_(4)+MgH_(2)+5 wt%Nb_(2)C possesses excellent cycling stability,with the reversible capacity only slightly reduced from 9.4 wt%for the 1st cycle to 9.3 wt%for the 20th ***_(2)C keeps stable in the composite and acts as an efficient catalyst for the li-Mg-B-H *** is believed that both the layered structure and the active Nb element contribu te to the enhanced hydrogen storage performances of the li-Mg-B-H *** work confirms that the Nb_(2)C MXene with layered stru cture has a significant enhancing impact on the li-Mg-B-H hydrogen storage materials,which is different from the bulk NbC.

关键词： Hydrogen storage lithium borohydride Magnesium hydride Nb_(2)C MXene

Pure-high-even-order dispersion bound solitons complexes in ultra-fast fiber lasers

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light(Science & Applications) 2024年第5期13卷 957-972页

作者： Ying Han Bo Gao Honglin wen Chunyang Ma Jiayu Huo Yingying li Luyao Zhou Qi li ge Wu lie liu College of Communication Engineering Jilin UniversityChangchun 130012China. Research Center of Circuits and Systems Peng Cheng LaboratoryShenzhen 518055China. International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education Institute of Microscale OptoelectronicsShenzhen UniversityShenzhen 518060China. College of Electronic Science and Engineering Jilin UniversityChangchun 130012China

Temporal solitons have been the focus of much research due to their fascinating physical *** solitons can form bound states,which are fundamentally crucial modes in fiber laser and present striking analogies with their matter molecules counterparts,which means they have potential applications in large-capacity transmission and alloptical information *** traditionally,second-order dispersion has been the dominant dispersion for conventional solitons,recent experimental and theoretical research has shown that pure-high-even-order dispersion(PHEOD)solitons with energy-width scaling can arise from the interaction of arbitrary negative-even-order dispersion and Kerr *** these advancements,research on the bound states of PHEOD solitons is currently *** this study,we obtained PHEOD bound solitons in a fiber laser using an intra-cavity spectral pulse shaper for high-order dispersion ***,we experimentally demonstrate the existence of PHEOD solitons and PHEOD bound solitons with pure-quartic,-sextic,-octic,and-decic *** simulations corroborate these experimental ***,vibrating phase PHEOD bound soliton pairs,sliding phase PHEOD bound soliton pairs,and hybrid phase PHEOD bound tri-soliton are discovered and *** results broaden the fundamental understanding of solitons and show the universality of multi-soliton patterns.

关键词： dispersion solitons soliton

Effect of V on high-temperature mechanical properties of Al-5Cu-1.5Ni alloy

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Journal of Iron and Steel Research International 2024年第7期31卷 1792-1810页

作者： Jun-ge Cui Yong-bin Chen Xiang-zhou Fu Hai-long Yang Han-zhang Wang An-min li li-wen Pan School of Resources Environment and MaterialsGuangxi UniversityNanning 530004GuangxiChina State Key Laboratory of Featured Metal Materials and life-Cycle Safety for Composite Structures Nanning 530004GuangxiChina Guangxi Higher Education Key Laboratory of Advanced Materials Nanning 530004GuangxiChina

The effects of V on microstructure,room temperature,and high-temperature(350℃)mechanical properties of Al-5Cu-1.5Ni alloy were *** results show that Al-5Cu-1.5Ni-xV alloys mainly compriseα-Al,Al_(2)Cu,Al_(3)(Ni,Cu)_(2),Al_(3)Ni,Al7Cu4Ni,and Al_(10)V *** addition of V can signally refineα-Al dendritic structure,which improves the mechanical properties of the as-cast alloys at room and high *** T6 heat treatment(540℃/12 h/WC+170℃/8 h/AC),α-Al,network skeleton structure Al_(3)(Ni,Cu)_(2),and Al_(10)V phases all show coarsening,while the fine nano-θ'-Al_(2)Cu precipitated from the matrix and dispersed *** introduction of V promotes the quantity of precipitatedθ'-Al_(2)Cu *** ultimate tensile strength of the heat-treated alloys at room and high temperatures is greatly improved compared to that of the as-cast *** highest high-temperature ultimate tensile strength of the alloys with V is 111.8 MPa,21.5%higher than that of the base *** analysis shows that the improvement of high-temperature mechanical properties after heat treatment is mainly due to the introduction of V,increasing the precipitated quantity of nano-θ'-Al_(2)Cu particles and improving its thermal *** dispersion strengthening effect ofθ'-Al_(2)Cu particles exceeds the weakening effect of other adverse microstructures on the mechanical properties.

关键词： Al-Cu-Ni-V alloy θ'-Al_(2)Cu phase Heat treatment Microstructure Mechanical property

Comparative analysis of the short and medium-term efficacy of the Da Vinci robot versus laparoscopic total mesangectomy for rectal cancer

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World Journal of Gastrointestinal Surgery 2024年第6期16卷 1681-1690页

作者： Wei-ge Gao wen Shi Xu-Chen Gong Zhi-wen li Yiminjiang Tuoheti Department of Anorectal Surgery People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqi 830001Xinjiang Uygur Autonomous RegionChina Department of Anus and Intestine Surgery Cancer Hospital of Chinese Academy of Medical SciencesBeijing 100084China

BACKGROUND The Da Vinci robot-assisted surgery technique has been widely used in laparo-scopic mesangectomy for rectal ***,the short-term efficacy of these procedures compared to traditional laparoscopic surgery remains *** purpose of this study was to compare and analyze the short-and medium-term efficacy of Da Vinci robot and laparoscopic surgery in total mesangectomy(TME)for rectal cancer,so as to provide guidance and reference for clinical *** To investigate the safety and long-term efficacy of robotic and laparoscopic total mesorectal resection for the treatment of rectal *** The clinicopathologic data of 240 patients who underwent TME for rectal cancer in the Anorectal Department of People’s Hospital of Xinjiang Uygur Autonomous Region from August 2018 to March 2023 were retrospectively *** them,112 patients underwent laparoscopic TME(L-TME)group,and 128 patients underwent robotic TME(R-TME)*** intraoperative,postoperative,and follow-up conditions of the two groups were *** The conversion rate of the L-TME group was greater than that of the R-TME group(5.4%vs 0.8%,χ^(2)=4.417,P=0.036).The complication rate of the L-TME group was greater than that of the R-TME group(32.1%vs 17.2%,χ^(2)=7.290,P=0.007).The percentage of positive annular margins in the L-TME group was greater than that in the R-TME group(7.1%vs 1.6%,χ^(2)=4.658,P=0.031).The 3-year disease-free survival(DFS)rate and overall survival(OS)rate of the L-TME group were lower than those of the R-TME group(74.1%vs 85.2%,χ^(2)=4.962,P=0.026;81.3%vs 91.4%,χ^(2)=5.494,P=0.019);in patients with American Joint Committee on Cancer stage Ⅲ DFS rate and OS rate in the L-TME group were significantly lower than those in the R-TME group(52.5%vs 76.1%,χ^(2)=5.799,P=0.016;65.0%vs 84.8%,χ^(2)=4.787,P=0.029).CONCLUSION Compared with the L-TME group,the R-TME group had a better tumor prognosis and was more favorable for patients with rectal cancer

关键词： Rectal tumor Robots Laparoscopy Total mesangectomy Survival prognosis Retrospective analysis

MULTIRATE TIME ITERATIVE SCHEME WITH MULTIPHYSICS FINITE ELEMENT METHOD FOR A NONliNEAR POROELASTICITY

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Journal of Computational Mathematics 2024年第2期42卷 597-616页

作者： Zhihao ge Hairun li Tingting li School of Mathematics and Statistics Henan UniversityKaifengHenan 475004China

In this paper,a multirate time iterative scheme with multiphysics finite element method is proposed and analyzed for the nonlinear poroelasticity *** original problem is reformulated into a generalized nonlinear Stokes problem coupled with a diffusion problem of a pseudo pressure field by a new multiphysics approach.A multiphysics finite element method is adopted for the spatial discretization,and the generalized nonlinear Stokes problem is solved in a coarse time step and the diffusion problem is solved in a finer time *** proposed algorithm is a decoupled algorithm,which is easily implemented in computation and reduces greatly computation *** stability analysis and the convergence analysis for the multirate iterative scheme with multiphysics finite element method are *** numerical tests are shown to demonstrate and validate the analysis results.

关键词： Nonlinear poroelasticity model Multiphysics finite element method Multirate iterative scheme

Flocking Effects of the Stochastic Cucker-Smale System with Noise

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Journal of Systems Science & Complexity 2024年第5期37卷 1978-1994页

作者： HAN Ning ge Bin li lin School of Mathematical Sciences Harbin Engineering UniversityHarbin 150001China

In this paper,the flocking behavior of a Cucker-Smale model with a leader and noise is studied in a finite *** authors present a Cucker-Smale system with two nonlinear controls for a complex network with stochastic synchronization in *** on the finite-time stability theory of stochastic differential equations,the sufficient conditions for the flocking of stochastic systems in a finite time are obtained by using the Lyapunov function ***,the numerical simulation of the particle system is carried out for the leader and noise,and the correctness of the results is verified.

关键词： Cucker-Smale model flocking behavior leader noise

Single Ti atoms coupled with Ti-O clusters enable low temperature hydrogen cycling by sodium alanate

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Rare Metals 2024年第6期43卷 2671-2681页

作者： Zhuang-He Ren Xin Zhang wen-Xuan Zhang Zhen-Guo Huang li-Mei Yang Ya-Xiong Yang Zheng-Long li Juan li wen-Ping Sun Ming-Xia Gao Hong-ge Pan Yong-Feng liu State Key Laboratory of Silicon and Advanced Semiconductor Materials Zhejiang UniversityHangzhou 310027China School of Materials Science and Engineering Zhejiang UniversityHangzhou 310058China School of Civil&Environmental Engineering.University of Technology Sydney Sydney2007 NSWAustralia Institute of Science and Technology for New Energy Xi'an Technological UniversityXi'an 710021China College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China

Ti-based catalysts are known to improve the hydrogen storage performance of NaAlH4by facilitating the dissociation/recombination of H-H and Al-H *** catalytic activity of metallic Ti species strongly depends on its particle size and *** clusters and even single atoms are therefore highly desirable,but their controllable fabrication has been highly *** rein,we demonstrate a novel facile sonochemical synthesis of a Ti-O clusters featuring single Ti atom catalyst at room *** reducing TiCl_(4)by MgBu_(2)with ultrasound instead of heating as driving force,numerous single Ti atoms coupled with Ti-O clusters with Ti loading on graphene(Ti_(1)/Ti-O@G)up to 22.6 wt%have been successfully *** prepared Ti_(1)/Ti-O@G contributes high reactivity and superior catalytic activity,therefore enabling full dehydrogenation of NaAlH_(4)at 80℃in thermogravimetric mode and re-hydrogenation at 30℃and 10 MPa with 4.9 wt% H_(2).This fact indicates for the first time that single Ti atom catalyst with high loading is highly effective in catalyzing hydrogen cycling of NaAlH4at remarkably reduced temperatures.

关键词： Hydrogen storage Nanocatalysis Transition metals Single Ti atoms NaAlH_(4)