检索结果-南通市图书馆

同方期刊数据库

两类水稻Nramp5基因突变体衍生系在镉污染稻田中糙米镉锰积累特征分析

在线全文

学校读者我要写书评

暂无评论

杂交水稻 2024年第1期39卷 17-26页

作者：宋嘉俊翁绿水李锦江邓力华王学华肖国樱湖南农业大学农学院湖南长沙410128 中国科学院亚热带农业生态研究所/亚热带农业生态过程重点实验室湖南长沙410125

以来源于珞红4A的Nramp5基因缺失突变型、来源于lcd1的Nramp5基因点突变型及缺失与点突变的杂合体为材料,比较在有效镉(Cd)含量为0.58 mg/kg(pH值5.3)的中度污染稻田种植条件下不同突变类型和遗传背景材料的糙米Cd和锰(Mn)含量。结果表... 详细信息

以来源于珞红4A的Nramp5基因缺失突变型、来源于lcd1的Nramp5基因点突变型及缺失与点突变的杂合体为材料,比较在有效镉(Cd)含量为0.58 mg/kg(pH值5.3)的中度污染稻田种植条件下不同突变类型和遗传背景材料的糙米Cd和锰(Mn)含量。结果表明,遗传背景不影响由Nramp5基因缺失或点突变所赋予的糙米Cd和Mn低积累特性;Nramp5基因缺失突变与点突变的杂合体的糙米Cd含量为0.082mg/kg,显著低于含野生型Nramp5基因的对照品种9311(2.345 mg/kg),且与Nramp5基因缺失或点突变的纯合体相比,Cd含量和Mn含量均无显著差异;Nramp5基因缺失纯合体、点突变纯合体及缺失与点突变杂合体的糙米Cd含量均符合国家食品安全标准(≤0.2 mg/kg)。因此,来源于珞红4A的Nramp5基因缺失突变的亲本与来源于lcd1的Nramp5基因点突变的亲本配组,其杂交种仍然具有糙米Cd低积累特征,本研究为杂交水稻低镉积累分子育种提供了重要参考数据。

关键词：水稻糙米镉锰 Nramp5基因突变低镉积累

Study on Chromosome Doubling for Haploid Produced by Wheat×Maize Crossing

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2002年第5期1卷 486-490页

作者： CHEN Xin-min, ZHANG Wen-xiang, GUI Shu-Ian and CHEN Xiao(Institute of Crop breeding and Cultivation , Chinese Academy of Agricultural Sciences , Beijing 100081 , P. R . China) Institute of Crop breeding and Cultivation Chinese Academy of Agricultural Sciences Beijing 100081 P. R. China

There is no spontaneous chromosome doubling in haploid plants produced by wheat X maize crossing. In order to obtain doubled haploid, two chromosome doubling methods were used. Results showed that: After adding colchicine solution directly into a medium for young embryos that had been cultured 7 days, frequencies of embryo germination in colchicine concentrations of 50mg/l, 100mg/l and 200mg/l were 32.1% , 26.4% and 16.3% , respectively, and frequencies of chromosome doubling were 85.3% , 100% and 50.0% , respectively. But in the control without colchicine, the frequency of embryo germination was 67.4% and no seed was setting. As the time of colchicine treatment increased from 24 to 72 hours, the frequency of embryo germination was reduced, and 24 hours had better results. After soaking seeding crowns and roots with colchicine solution of 500mg/l, 750mg/l and 1 000mg/l for 5 hours, the frequencies of doubling were 89.6%, 76.0% and 73.3%, respectively. By soaking crowns and roots of strong seedings with 500mg/l colchicine solution, the frequency and efficiency of doubling were 98.2% and 93.2% , respectively.

关键词： Wheat X maize crosses Haploid Doubled haploid

叶果比对‘新新2号’核桃叶片光合特征的影响

在线全文

学校读者我要写书评

暂无评论

分子植物育种 2024年第16期22卷 5444-5451页

作者：陈虹孙守霞张述斌吕威周光辉杨丽潘存德新疆农业大学林学与风景园林学院新疆教育厅干旱区林业生态与产业技术重点试验室乌鲁木齐830052

源库关系通过对光合同化物的运输、分配及光能的捕获协调果树产量,针对新疆早实核桃普遍存在叶果比不合理的现象,本研究以‘新新2号’核桃为研究对象,分别于花后30、60、90、120 d进行不同叶果比下核桃叶片光合特性测定。研究发现,结果... 详细信息

源库关系通过对光合同化物的运输、分配及光能的捕获协调果树产量,针对新疆早实核桃普遍存在叶果比不合理的现象,本研究以‘新新2号’核桃为研究对象,分别于花后30、60、90、120 d进行不同叶果比下核桃叶片光合特性测定。研究发现,结果枝叶片数目为1~2片时,叶片P_(n)、G_(s)值随着叶果比的增大而增大;叶片数目为3~4片时,叶片的P_n值较高并出现峰值且2果和3果结果枝上叶片P_(n)之间差异不显著;叶片数目为5片时,叶果比与P_(n)、G_(s)值呈显著负相关。结果表明,核桃叶果比为(1~2)l:(2~3)F时,叶片的光合能力较弱,叶果比为(3~5)l:(1~2)F时叶片的光合能力较强。合适的叶果比范围内,即果实数量一定,叶片数量为3~5时,叶果比的改变对叶片光合作用无显著影响。本研究为核桃合理的叶果比提供参考,为核桃树整形修剪、确定合理负载量提供理论依据。

关键词：核桃叶果比叶片光合特征

粳稻资源蛋白质组分及其与稻米品质性状的关联研究

在线全文

学校读者我要写书评

暂无评论

中国粮油学报 2024年第6期39卷 43-50页

作者：鞠鑫月朱子业马兆惠程海涛王先俱吕文彦沈阳农业大学农学院沈阳110161 辽宁省水稻研究所沈阳110101

以适应辽宁省熟期且外观与加工品质符合育种目标的92份特异性粳稻资源为试材,分析其蛋白质组分与淀粉组分含量变异特点,以及这些组分与其他胚乳性状的关系。继而选择峰值黏度和崩解值高、回冷值低的16份材料作为食味优良材料,进一步研... 详细信息

以适应辽宁省熟期且外观与加工品质符合育种目标的92份特异性粳稻资源为试材,分析其蛋白质组分与淀粉组分含量变异特点,以及这些组分与其他胚乳性状的关系。继而选择峰值黏度和崩解值高、回冷值低的16份材料作为食味优良材料,进一步研究了蛋白组分、淀粉组分与稻米食味的关系。结果表明,所研究的粳稻资源在蛋白质组分和淀粉组分变异丰富。其中,清蛋白含量高的品种AAC和Fb_(3)较低,而Fa和Fb_(1)+Fb_(2)较高。清蛋白与峰值黏度、最低黏度、崩解值、终值黏度、回冷值均呈现极显著负相关。对于优良食味品种,谷蛋白含量与食味值、外观、黏度、平衡度呈显著或极显著负相关关系,与硬度呈显著正相关。由此发现,所研究的品种资源中,存在丰富的蛋白质组分及淀粉成分变异,且清蛋白对优良食味有促进作用,而谷蛋白含量则可能增加米饭硬度,降低米饭平衡度,从而劣化米饭食味。此外,崩解值和回冷值可作为预测米饭食味品质的有效指标。

关键词：稻米蛋白质组分稻米品质

不同铵硝比对高山杜鹃侧枝生长和生理特性影响

在线全文

学校读者我要写书评

暂无评论

核农学报 2024年第4期38卷 785-793页

作者：高研彭绿春李世峰解玮佳宋杰张露熊俊芬赵正雄云南农业大学资源与环境学院云南昆明650201 云南省农业科学院花卉研究所/国家观赏园艺工程技术研究中心/云南省花卉育种重点实验室/云南花卉技术工程研究中心云南昆明650205

为探究氮素形态对高山杜鹃侧枝生长的影响,以3年生盆栽高山杜鹃粉精灵为试验材料,单一氮源处理为对照,采用盆栽方式,研究5种不同氮素形态配比供应(铵硝比例分别为T1:100∶0,T2:75∶25,T3:50∶50,T4:25∶75和T5:0∶100)对其营养生长期侧... 详细信息

为探究氮素形态对高山杜鹃侧枝生长的影响,以3年生盆栽高山杜鹃粉精灵为试验材料,单一氮源处理为对照,采用盆栽方式,研究5种不同氮素形态配比供应(铵硝比例分别为T1:100∶0,T2:75∶25,T3:50∶50,T4:25∶75和T5:0∶100)对其营养生长期侧枝生长、光合特性、代谢酶活性及营养元素积累的影响。结果表明,不同氮素形态下粉精灵不同生长期侧枝干物质积累量、苗高增量和侧枝长度均有所差异,以侧芽萌发期T3处理侧枝干物质积累量最高,T2处理在嫩侧枝期、侧枝半木质化期的苗高增量和侧枝长度均显著高于其他处理。侧枝半木质化期,与T1处理相比,T2处理显著提高净光合速率、蒸腾速率、气孔导度、水分利用率及磷含量,降低胞间二氧化碳浓度;叶片Fe元素吸收量以T1处理最高,Mg元素吸收量以T5处理最高。此外,在不同生长期,硝酸还原酶活性均随硝态氮含量的增加而增加,以嫩侧枝期酶活性最高。综上,不同氮素形态对高山杜鹃粉精灵侧枝生长和生理特性的影响有所差异,且混合氮源处理较单一氮源处理更有利于促进侧枝生长,以铵硝比例75∶25促进侧枝生长及提高养分积累和光合效能效果最好。本研究为筛选适合高山杜鹃生长发育的氮素施用方法提供了理论依据。

关键词：高山杜鹃氮素形态侧枝生长生理特性营养元素

Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem l.)

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2008年第6期18卷 697-705页

作者： Hualing Wu,Zhongfu Ni,Yingyin Yao,Ganggang Guo,Qixin Sun* Department of Plant Genetics&breeding and State Key laboratory for Agrobiotechnology,Key laboratory of Crop Heterosis and Utilization,Ministry of Education,Key laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture/Beijing Key laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100094,China Department of Plant Genetics & breeding and State Key laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization Ministry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

WRKY proteins are involved in various physiological processes,including biotic and abiotic stress responses,hormone responses and ***,no systematic identification,expression and function analysis of WRKY genes in wheat were *** this study,we isolated 15 wheat cDNAs with complete open reading frame(ORF)encoding putative WRKY proteins using in silico *** analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY *** analysis revealed that most genes expressed drastically in leaf,except TaWRKY10 which expressed in crown *** genes were strongly up-reg-ulated with the senescence of *** genes were responsive to low temperature,high temperature,NaCl or PEG ***-over,dierential expression patterns were also observed between wheat hybrid and its parents,and some genes were more responsive to PEG treatment in the *** results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic *** the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids.

关键词： Wheat WRKY transcription factor Gene cloning Gene expression Heterosis

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

不同土壤处理方式对胡杨幼苗根系生长的影响

科学技术与工程 2024年第15期24卷 6207-6216页

作者：邵帅高安岭左彦平吕慧陈利俊敖日格乐李景文北京林业大学生态与自然保护学院北京100083 内蒙古额济纳胡杨林国家级自然保护区管理局额济纳旗735400 额济纳旗国有林场额济纳旗735400 内蒙古贺兰山国家级自然保护区管理局阿拉善左旗750306

为明晰植物幼苗在不同土壤质地中的生长适应策略,探讨利用不同土壤处理方式调控植株幼苗生长发育和提高人工育苗效率的可能。将苗圃土壤分别设置为平床深耕、起垄和平床浅耕3种处理,研究不同处理下当年生胡杨幼苗生长季的生长动态和根... 详细信息

为明晰植物幼苗在不同土壤质地中的生长适应策略,探讨利用不同土壤处理方式调控植株幼苗生长发育和提高人工育苗效率的可能。将苗圃土壤分别设置为平床深耕、起垄和平床浅耕3种处理,研究不同处理下当年生胡杨幼苗生长季的生长动态和根系变化特征。结果表明:7月份是胡杨幼苗生长关键期,比根长能够作为反映其生长情况的重要指标,土壤处理方式影响着胡杨幼苗地上、地下生物量分配以及根系形态,深耕对幼苗根系生长有促进作用,为胡杨苗圃土壤处理方式的选择提供参考价值。

关键词：胡杨耕作方式幼苗生长根系形态

Molecular mapping of QTls for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum l.)

在线全文

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2007年第10期17卷 1177-1184页

作者： li Zhenxing, Ni Zhongfu, Peng Huiru, liu Zhiyong, Nie Xiuling, Xu Shengbao, liu Gang and Sun Qixin(Key laboratory of Crop Heterosis and Utilization and State Key laboratory for Agrobiotechnology, Key laboratory of Crop Genomics and Genetic Improvement, Beijing Key laboratory of Crop Genetic Improvement, Department of Plant Genetics & breeding, China Agricultural University, Beijing 100094, China) Key laboratory of Crop Heterosis and Utilization Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China Key laboratory of Crop Genomics and Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China Beijing Key laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China

Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTls) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTls (lOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTls were distributed on 14 chromosomes, with each of the 5 QTls explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTls. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together.

关键词： QTls roots wheat seedling RIl P-deficiency.

Towards estimating shade response of bermudagrass(Cynodon spp.)using field-based photosynthetic properties

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Grass Research 2022年第1期2卷 20-25,I0001-I0004页

作者： Jonathon l.Fox David Jespersen lisa l.Baxter John l.Snider Marc W.van Iersel Brian M.Schwartz Department of Crop and Soil Sciences University of Georgia2360 Rainwater Rd.TiftonGA 31794USA Department of Crop and Soil Sciences University of Georgia1109 Experiment St.GriffinGA 30223USA Department of Horticulture University of Georgia1111 Plant Science BuildingAthensGA 30602USA

Shade tolerance is a highly desirable trait when breeding new bermudagrass(Cynodon spp.)lines,but current screening methods can take many years to *** is a clear need for large-scale turfgrass breeding programs to reliably,accurately,and quickly predict shade tolerance in their *** objectives of this research were to:(i)build custom chambers to reproducibly estimate photosynthetic characteristics from turfgrass canopies at different light intensities and(ii)determine which photosynthetic characteristics are indicative of past shade performance.A custom-built chamber was constructed to determine average photosynthetic characteristics for the whole plant by studying the turfgrass canopy at natural leaf angles for light *** tolerant(11-T-56)and shade sensitive(Tifway)bermudagrass cultivars were used to examine the effectiveness of the chamber within an array of photosynthetic characteristics when the grasses were grown in full-sun and 73%shade *** compensation point,chlorophyll content,quantum yield,dark respiration rate,and maximum quantum yield of photosystem II were evaluated in this *** on the results of this study,the authors recommend that light compensation point or maximum quantum yield be further evaluated as an accurate indicator of shade tolerance when performed in the field on spring days with grasses grown in full sun or 73%shade *** Rd rates and ambient temperatures on spring days appear to minimize unexplained variance in the data,which would allow researchers to better detect genotypic differences during this season.

关键词： breeding field estimating