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Single-cell RNA sequencing of mitoticarrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1305-1325页

作者： Hyeon Jeong Choi kyung Min jung Deivendran Rengaraj kyung Youn Lee Eunhui Yoo Tae Hyun Kim Jae Yong Han Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826South Korea Department of Animal Science Pennsylvania State UniversityState CollegePA 16801USA

Background:Germ cell mitotic arrest is conserved in many vertebrates,including birds,although the time of entry or exit into quiescence phase *** arrest is essential for the normal differentiation of male germ cells into spermatogonia and accompanies epigenetic reprogramming and meiosis inhibition from embryonic development to ***,mitotic arrest was not well studied in chickens because of the difficulty in obtaining pure germ cells from relevant developmental ***:We performed single-cell RNA sequencing to investigate transcriptional dynamics of male germ cells during mitotic arrest in DAZL::GFP *** differentially expressed gene analysis and K-means clustering to analyze cells at different developmental stages(E12,E16,and hatch),we found that metabolic and signaling pathways were regulated,and that the epigenome was reprogrammed during mitotic *** particular,we found that histone H3K9 and H3K14 acetylation(by HDAC2)and DNA demethylation(by DNMT3B and HELLS)led to a transcriptionally permissive chromatin ***,we found that global DNA demethylation occurred gradually after the onset of mitotic arrest,indicating that the epigenetic-reprogramming schedule of the chicken genome differs from that of the mammalian *** hypomethylation persisted after hatching,and methylation was slowly re-established 3 weeks ***:We found a unique epigenetic-reprogramming schedule of mitotic-arrested chicken prospermatogonia and prolonged hypomethylation after *** will provide a foundation for understanding the process of germ-cell epigenetic regulation in several species for which this process is not clearly *** findings on the biological processes related to sex-specific differentiation of prospermatogonia could help studying germline development in vitro more elaborately.

关键词： DAZL::GFP chickens DNA methylation Epigenetic reprogramming Histone acetylation Male germ cell Mitotic arrest Prospermatogonia Single-cell RNA sequencing

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Phosphorylated p70S6K in urothelial carcinoma of the tumor recurrence noninvasive low-grade bladder： correlation with

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Asian Journal of Andrology 2014年第4期16卷 611-617页

作者： Soon-Ja Kim jung Hoon Kim Hui Seok jung Tae-Jin Lee kyung Mee Lee In Ho Chang Department of Convergence Medicine and Pharmaceutical Biosciences Chung-Ang University Graduate School Seoul Department of Urology Department of Pathology Chung-Ang University College of Medicine Seoul Korea.

We investigated whether inhibiting phosphorylated p70S6K （p-p70S6K） suppresses the proliferation and growth of noninvasive low-grade urothelial carcinoma （LG-URCa） in vitro and whether p-p70S6K can serve as a predictive biomarker for the recurrence of noninvasive LG-URCa of the bladder in patients. We constructed a tissue microarray （TMA） for 95 LG-URCa and 35 benign urothelium samples and performed immunohistochemical staining for p-p70S6K and p-4E-BP1. A Cox regression model was used to investigate the predictive factors for recurrence of LG-URCa. We investigated the dose-dependent antiproliferative effect of rapamycin, its antiproliferative effect and the growth-inhibition effect of p70S6K siRNA transfection in RT4 and 253J cell lines. The pT1 staged group （P 〈 0.05; hazard ratio （HR）, 2.415） and the high p-p70S6K staining group （P 〈 0.05; HR, 2.249） were independent factors for predicting recurrence. Rapamycin inhibited RT4 and 253J cell proliferation in a dose-dependent manner （r = -0.850, P 〈 0.001 in RT4 cells; r = -0.835, P 〈 0.001 in 253J cells）. RT4 and 253J cell proliferation and growth were inhibited by the transfection of p70S6K siRNA and rapamycin, respectively （P 〈 0.05）. Transfection of p70S6K siRNA resulted in inhibitory effects on cell proliferation and growth that were similar to those of rapamycin. Our results suggest that inhibiting p70S6K phosphorylation is important to prevent recurrence and that p70S6K phosphorylation can be used as a molecular biomarker to predict recurrence of certain LG-URCa of the bladder.

关键词： bladder cancer p7OS6K

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First synthesis and in vitro biological assessment of isosideroxylin,6,8-dimethylgenistein and their analogues as nitric oxide production inhibition agents

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Chinese Chemical Letters 2017年第5期28卷 1114-1118页

作者： Jong-Woon jung Kongara Damodar Jin-kyung Kim Jong-Gab Jun Department of Chemistry and Institute of Applied Chemistry Hallym University Department of Biomedical Science College of Natural ScienceCatholic University of Daegu

A modular and efficient synthesis of the biologically significant C-methylisoflavones isosideroxylin（1）,6,8-dimethylgenistein（2） and their analogues（3-8） is established for the first *** synthesis is realized in 7-8 steps in overall yields of 16%-24%from commercially inexpensive phloroglucinol and features a high yielding Vilsmeier-Haack reaction,Friedel-Crafts acylation,Gammill＇s protocol and Suzuki coupling as the pivotal ***,these compounds evaluated for their inhibitory potency on the production of nitric oxide（NO） in lipopolysaccharide（LPS）-activated RAW-264.7 cells as an indicator of anti-inflammatory *** results showed that all the compounds decreased NO production in a dose-dependent manner without marked cytotoxicity and IC_（50） values are found in the range of 10.17-33.88 μmol/*** note,compounds 3 followed by 1,7 and 8 show comparable inhibitory activity with positive control（N-monomethyl-L-arginine,L-NMMA）.

关键词： Isosideroxylin 6 8-Dimethylgenistein Vilsmeier-Haack reaction Suzuki coupling Nitric oxide(NO) Anti-inflammatory

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Analysis of opto-thermal interaction of porcine stomach tissue with 808-nm laser for endoscopic submucosal dissection

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Journal of Innovative Optical Health Sciences 2015年第6期8卷 81-91页

作者： Seongjun Kim M.Tamim Hossain Don haeng Lee jung kyung Kim Department of Mechanical Engineering Graduate School Kookmin University Seoul 136-702Republic of Korea Department of Integrative Biomedical Science and Engineering Graduate SchoolKookmin University Seoul 136-702Republic of Korea Division of Gastroenterology Department of Internal Medicine Inha University School of Medicine National Center of Efficacy Evaluation for the Development of Health Products Targeting Digestive Disorders Utah-Inha DDS&Advanced Therapeutics Research Center 27 Inhang-ro Jung-guIncheon 400-711Republic of Korea Department of Mechanical Engineering Kookmin University Seoul 136-702 Republic of Korea

In endoscopic submucosal dissection(ESD),the narrow gastrointestinal space can cause difficulty in surgical *** ablation apparatuses with high-power CO_(2) lasers or Nd:YAG lasers have been developed to facilitate endoscopic surgical *** studied the interaction of 808-nm laser light with a porcine stomach tissue,with the aim of developing a therapeutic medical device that can remove lesions at the gastrointestinal wall by irradiating a near-infrared laser light incorporated in an endoscopic *** perforation depths at the porcine fillet and the stomach tissues linearly increased in the range of 2–8mm in proportion to the laser energy density of 63.7–382 kJ/cm^(2).Despite the distinct structural and compositional di®erence,the variation of the perforation depth between the stomach and the fillet was not found at 808-nm wavelength in our *** further studied the laser–tissue interaction by changing the concentration of the methyl blue solution used conventionally as a submucosal fluidic cushion(SFC)in ESD *** temperature of the mucosal layer increased more rapidly at higher concentration of the methyl blue solution,because of enhanced light absorption at the SFC *** insertion of the SFC would protect the muscle layer from thermal *** confirmed that more effective laser treatment should be enabled by tuning the opto-thermal properties of the *** study can contribute to the optimization of the driving parameters for laser incision techniques as an alternative to conventional surgical interventions.

关键词： Near-infrared laser surgical intervention laser-tissue interaction submucosal fluid cushion temperature distribution

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Multifunctional GelMA platforms with nanomaterials for advanced tissue therapeutics

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Bioactive Materials 2022年第2期7卷 267-295页

作者： Amal George Kurian Rajendra KSingh Kapil DPatel jung-Hwan Lee hae-Won Kim Institute of Tissue Regeneration Engineering(ITREN) Dankook UniversityCheonan31116Republic of Korea Department of Nanobiomedical Science&BK21 NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonan31116Republic of Korea Biomaterials and Tissue Engineering UCL Eastman Dental InstituteLondonWC1X8LDUK Department of Biomaterials Science School of DentistryDankook UniversityCheonan31116Republic of Korea UCL Eastman-Korea Dental Medicine Innovation Centre Dankook UniversityCheonan31116Republic of Korea Cell&Matter Institute Dankook UniversityCheonan31116Republic of Korea Department of Regenerative Dental Medicine College of DentistryDankook UniversityCheonan31116Republic of Korea Mechanobiology Dental Medicine Research Center Dankook UniversityCheonan31116Republic of Korea

Polymeric hydrogels are fascinating platforms as 3D scaffolds for tissue repair and delivery systems of therapeutic molecules and *** others,methacrylated gelatin(GelMA)has become a representative hydrogel formulation,finding various biomedical *** efforts on GelMA-based hydrogels have been devoted to combining them with bioactive and functional nanomaterials,aiming to provide enhanced physicochemical and biological properties to *** benefits of this approach are multiple:i)reinforcing mechanical properties,ii)modulating viscoelastic property to allow 3D printability of bio-inks,iii)rendering electrical/magnetic property to produce electro-/magneto-active hydrogels for the repair of specific tissues(e.g.,muscle,nerve),iv)providing stimuli-responsiveness to actively deliver therapeutic molecules,and v)endowing therapeutic capacity in tissue repair process(e.g.,antioxidant effects).The nanomaterial-combined GelMA systems have shown significantly enhanced and extraordinary behaviors in various tissues(bone,skin,cardiac,and nerve)that are rarely observable with *** we systematically review these recent efforts in nanomaterials-combined GelMA hydrogels that are considered as next-generation multifunctional platforms for tissue *** approaches used in GelMA can also apply to other existing polymeric hydrogel systems.

关键词： GelMA hydrogel Nanomaterials Multifunctional Therapeutics Tissue repair

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Prognosis of hepatocellular carcinoma expressing cytokeratin 19:Comparison with other liver cancers

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World Journal of Gastroenterology 2012年第34期18卷 4751-4757页

作者： jung Il Lee Jin-Woo Lee Joon Mee Kim Ja kyung Kim Hyun jung Chung Young Soo Kim Department of Internal Medicine School of MedicineInha UniversityIncheon 400-711South Korea Department of Pathology School of MedicineInha UniversityIncheon 400-711South Korea Department of Internal Medicine Yonsei University College of MedicineGangnam Severance HospitalSeoul 135-720South Korea

AIM:To investigate whether expressing biliary phenotype predicted poor outcome after the surgical treatment in primary liver cancers. METHODS:Out of 204 patients that underwent liver resection due to hepatocellular carcinoma (HCC), liver specimens of 70 patients with HCC were evaluated for biliary components by cytokeratin (CK) 19 immunostain (CK19 - HCC and CK19 + HCC). CK19 positivity was defined as membranous and/or cytoplasmic expression in ≥ 5% of tumor cells with moderate or strong intensity. Patients with other primary liver cancers, such as com- bined HCC and cholangiocarcinoma (cHCC-CC), intrahe- patic cholangiocarcinoma (ICC) who received curative liver resection, were also included in the study. Clinical characteristics of CK19-HCC and CK19 + HCC patients, including survival outcome after curative liver resection, were compared with that of cHCC-CC and ICC patients. RESULTS: The overall survival (OS) rate of CK19 - HCC(n = 49) after the curative surgical treatment was 90.7%, and 80.4% at 1 and 5 years after the resection. OS rate of CK19 + HCC (n = 21) was 74.3%, 28.9% and OS rate of cHCC-CC (n = 22) was 66.7%, 32.2% at 1 and 5 years after the surgery. For ICC (n = 19), 1 and 5-year-OS rate was 50.2% and 14.3% after the cura-tive resection. The OS rates of CK19 + HCC and cHCC-CC were significantly lower than that of CK19-HCC, but higher than the OS rate of ICC (P = 0.000). There was no statistically significant difference in OS rate between CK19 + HCC and cHCC-CC. The disease free survival (DFS) rate of CK19-HCC was 72.0% and 54.5% at 1 and 3 years after the surgical treatment. DFS rate of CK19 + HCC was 53.3%, 34.3% and DFS rate of cHCC- CC was 51.5%, 39.2% at 1 and 3 years after the resection. For ICC, 1 and 3-year-DFS rate was 28.0% and 14.0% after the curative resection. DFS rate of CK19-HCC was significantly higher than that of ICC (P = 0.017), but marginally higher than DFS rate of either CK19 + HCC or cHCC-CC (P = 0.097, P = 0.089, respec-tively).

关键词： Cytokeratin 19 Hepatocellular carcinoma Intrahepatic cholangiocarcinoma Liver cancers Hepa-tectomy

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A numerical investigation into the long-term behaviors of Fukushima-derived ^(137)Cs in the ocean

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Acta Oceanologica Sinica 2015年第12期34卷 37-43页

作者： ZHAO Chang WANG Gang QIAO Fangli WANG Guansuo jung kyung-Tae XIA Changshui Key Laboratory of Marine Science and Numerical Modeling the First Institute of Oceanography State Oceanic Administration Korea Institute of Ocean Science and Technology

The Fukushima nuclear accident in 2011 released large amounts of radionuclides, including ^（137）Cs, into the Pacific Ocean. A quasi-global ocean radioactive transport model with horizontal grid spacing of 0.5°×0.5° and 21 vertical layers was thereafter established to study the long-term transport of the Fukushima-derived ^（137）Cs in the *** simulation shows that the plume of ^（137）Cs would be rapidly transported eastward alongside the Kuroshio Current and its extensions. Contaminated waters with concentrations lower than 2 Bq/m3 would reach the west coast of North America 4 or 5 years after the accident. The ^（137）Cs tends to be carried, despite its very low concentration, into the Indian and South Pacific Oceans by 2016 via various branches of ocean ***, the ^（137）Cs concentrations in the western part of the North Pacific Ocean decrease rapidly with time. Up to now the highly contaminated waters have remained in the upper 400 m, showing no evidence of significant penetration to deeper layers.

关键词： Fukushima accident ^(137)Cs radioactivity model Kuroshio

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Self‑Powered Airflow Sensor Based on Energy Harvesting of Ventilation Air in Buildings

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Advanced Fiber Materials 2023年第5期5卷 1788-1798页

作者： Moon Hyun Chung Seunghwan Yoo Woo‑Nam jung Hakgeun Jeong kyung‑Hwa Yoo Department of Physics Yonsei University50 Yonsei‑RoSeodaemun‑GuSeoul 03722Republic of Korea Energy ICT Convergence Research Department Energy Efficiency Research DivisionKorea Institute of Energy Research152 Gajeong‑RoYuseong‑GuDaejeon 34129Republic of Korea Advanced Combustion Power Lab Energy Efficiency Research DivisionKorea Institute of Energy Research152Gajeong‑RoYuseong‑GuDaejeon 34129Republic of Korea Department of Mechanical Engineering Korea Advanced Institute of Science and Technology(KAIST)291 Daehak‑RoYuseongDaejeon 34141Republic of Korea

Heating,ventilation,and air conditioning(HVAC)systems account for one-third of the total energy consumption in office *** use of airflow measurements to control the operation of HVAC systems can reduce energy consumption;thus,a sensor capable of monitoring airflow in a duct system is *** nanogenerators(TENGs)can be utilized as self-powered sensors in airflow-driven TENGs(ATENGs)as self-powered *** employing ferroelectric materials and surface modifications,the surface charges of TENGs can be *** this study,fibrous-mat TENGs were prepared using ferroelectric materials consisting of poly(vinylidene fluoride-co-trifluoroethylene)(PVDF-TrFE)and polyamide 11(nylon-11).And these materials were subsequently ***(3,4-ethylenedioxythiophene):poly(styrenesulfonate)was added to PVDF-TrFE to enhance the ferroelectric crystalline phase.X-ray diffraction analysis revealed that this incorporation affects theβ*** addition,the surface of nylon-11 was modified using the electrospray technique for post-treatment,thereby improving the interfacial adhesion between the *** materials were then utilized in fibrous-mat ATENGs(FM-ATENGs)to demonstrate their practical *** FM-ATENGs can be effectively used in an Arduino airflowcheck sensor,showcasing their potential for application in HVAC systems,to enhance airflow control and energy efficiency.

关键词： HVAC Airflow-driven triboelectric nanogenerator Building energy harvesting Fibrous mat Airflow sensor

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Reinfection rate and endoscopic changes after successful eradication of Helicobacter pylori

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World Journal of Gastroenterology 2010年第2期16卷 251-255页

作者： Kum Hei Ryu Sun Young Yi Youn Ju Na Su jung Baik Su Jin Yoon hae-Sun jung Hyun Joo Song Center for Cancer Prevention and Detection National Cancer CenterMadu1-dongIlsandong-guGoyangsiGyeonggi-do410-769South Korea Departments of Internal Medicine Ewha Womans University School of Medicine911-1 Mokdong Yancheon-guSeoul158-710South Korea

AIM:To determine the long-term outcomes regarding reinfection with Helicobacter pylori(***)and endoscopic changes after successful *** eradication. METHODS:From June 1994 to January 2007,186 patients(M:F=98:88;mean age 50.0±11.4 years), in whom *** had been successfully eradicated, were *** mean duration of follow up was 41.2±24.0 mo. RESULTS:*** reinfection occurred in 58 patients (31.2%).The average annual reinfection rate was 9.1% per patient *** recurrence of peptic ulcer was detected at the follow up *** were no significant differences between the *** eradication regimens for the reinfection rate and no significant differences in endoscopic findings between the *** group and the ***-cured ***:The reinfection rate in Korea is 9.1% which represents a decreasing *** was no relationship between *** infection status and changes in endoscopic *** was also no recurrence or aggravation of ulcers.

关键词： Helicobacter pylori Eradication Reinfection Endoscopy

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A potential oncogenic role of the commonly observed E2F5 overexpression in hepatocellular carcinoma

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World Journal of Gastroenterology 2011年第4期17卷 470-477页

作者： Yuzhu Jiang Seon-Hee Yim Hai-Dong Xu Seung-Hyun jung So Young Yang hae-Jin Hu Chan-Kwon jung Yeun-Jun Chung Integrated Research Center for Genome Polymorphism and Department of Microbi-ology School of Medicine The Catholic University of KoreaSocho-gu Seoul 137-701 South Korea Integrated Research Center for Genome Polymorphism and Department of Medical Humanities and SocialSciences School of Medicine The Catholic University of Korea Socho-gu Seoul 137-701 South Korea Hospital Pathology Seoul St Mary's Hospi-tal Socho-gu Seoul 137-701 South Korea

AIM: To explore the expression pattern of E2F5 in primary hepatocellular carcinomas (HCCs) and elucidate the roles of E2F5 in hepatocarcinogenesis. METHODS: E2F5 expression was analyzed in 120 primary HCCs and 29 normal liver tissues by immunohistochemistry analysis. E2F5-small interfering RNA was transfected into HepG2, an E2F5-overexpressed HCC cell line. After E2F5 knockdown, cell growth capacity and migrating potential were examined. RESULTS: E2F5 was significantly overexpressed in primary HCCs compared with normal liver tissues (P = 0.008). The E2F5-silenced cells showed significantly reduced proliferation (P = 0.004). On the colony formation and soft agar assays, the number of colonies was significantly reduced in E2F5-silenced cells (P = 0.004 and P = 0.009, respectively). E2F5 knockdown resulted in the accumulation of G0/G1 phase cells and a reduction of S phase cells. The number of migrating/invading cells was also reduced after E2F5 knockdown (P = 0.021). CONCLUSION: To our knowledge, this is the first evidence that E2F5 is commonly overexpressed in primary HCC and that E2F5 knockdown significantly repressed the growth of HCC cells.

关键词： E2F5 E2F family Hepatocellular carcinoma Oncogene Small interfering RNA

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