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A parallel and scalable digital architecture for training support vector machines

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第8期11卷 620-628页

作者： kui-kang CAO Hai-bin SHEN Hua-feng CHEN Institute of VLSI Design Zhejiang University Hangzhou 310027 China Zhejiang University of Media and Communications Hangzhou 310027 China

To facilitate the application of support vector machines (SVMs) in embedded systems,we propose and test a parallel and scalable digital architecture based on the sequential minimal optimization (SMO) algorithm for training *** taking advantage of the mature and popular SMO algorithm,the numerical instability issues that may exist in traditional numerical algorithms are *** error cache updating task,which dominates the computation time of the algorithm,is mapped into multiple processing units working in *** results show that using the proposed architecture,SVM training problems can be solved effectively with inexpensive fixed-point arithmetic and good scalability can be *** architecture overcomes the drawbacks of the previously proposed SVM hardware that lacks the necessary flexibility for embedded applications,and thus is more suitable for embedded use,where scalability is an important concern.

关键词： Support vector machine (SVM) Sequential minimal optimization (SMO) Field-programmable gate array (FPGA) Scalable architecture

Identification of a sugar gustatory receptor and its effect on fecundity of the brown planthopper Nilaparvata lugens

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Insect Science 2019年第3期26卷 441-452页

作者： Wei-Wen Chen kui kang Pan Yang Wen-Qing Zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen University Guangzhou China

In insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. Gustatory stimuli from the environment are recognized by gustatory receptors. To date, little is known about the function of gustatory receptors in agricultural pest insects. In this study, we cloned a sugar gustatory receptor gene, N/Gr11, from the brown planthopper (BPH), Nilaparvata lugens (Stal), a serious pest of rice in Asia;we then identified its ligands, namely, fructose, galactose and arabinose, by calcium imaging assay. After injection of N/Gr11 doublestranded RNA, we found that the number of eggs laid by BPH decreased. Moreover, we found that N/Gr11 inhibited the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and promoted the phosphorylation of protein kinase B (AKT). These findings demonstrated that N/Gr11 could accelerate the fecundity of BPH through AMPK- and AKT-mediated signaling pathways. This is the first report to indicate that a gustatory receptor modulates the fecundity of insects and that the receptor could be a potential target for pest control.

关键词： brown planthopper fecundity gustatory receptors ligands sugar

Galactose-N/Gr11 inhibits AMPK phosphorylation byactivating the PI3K-AKT-PKF-ATPsignaling cascade viainsulin receptor and Gβγ

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Insect Science 2021年第3期28卷 735-745页

作者： Wei-Wen Chen kui kang Jun Lv Lei Yue Wen-Qing Zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina

As ligands of the sugar gustatory receptors,sugars have been known to activatethe insulin/insulin-like growth factor signaling pathway;however,the precise pathways thatare activated by the sugar-bound gustatory receptors in insects remain *** this study,we aimed to investigate the signaling cascades activated by NlGr11,a sugar gustatory re-ceptor in the brown planthopper Nilaparvata lugens(Stal),and its ***-boundNGrl1(galactose-NIGr11)activated the-phosphatidylinositol 3-kinase(PI3K)-AKT signaling cascade via insulin receptor(InR)and Gβγin *** addition,galactose-NlGr11inhibited the adenosine monophosphate-activated protein kinase(AMPK)phosphorylationby activating the AKT-phosphofructokinase(PFK)-ATP signaling cascade in ***-tantly,the InR-PI3K-AKT-PFK-AKT signaling cascade was activated and the AMPKphosphorylation was inhibited after feeding the brown planthoppers with galactose ***,these findings confirm that NIGr1l can inhibit AMPK phosphorylationby activating the PI3K-AKT-PFK-ATP signaling cascades via both InR and GBy whenbound to ***,our study provides novel insights into the signaling pathwaysregulated by the sugar gustatory receptors in insects.

关键词： AMPK galactosc Gβγ IlS pathway sugar gustatory receptor

FoxO directly regulates the expression of TOR/S6K and vitellogenin to modulate the fecundity of the brown planthopper

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Science China(Life Sciences) 2021年第1期64卷 133-143页

作者： Yi Dong Weiwen Chen kui kang Rui Pang Yipei Dong Kai Liu Wenqing Zhang State Key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhou510275China

As a conserved transcription factor,FoxO plays a crucial role in multiple physiological processes in vivo,including stress resistance,longevity,growth and *** studies on FoxO have focused on human,mouse,Drosophila melanogaster and Caenorhabditis elegans,while there are few reports on agricultural pests and little is known about how FoxO modulates insect *** Asia,the brown planthopper(BPH)Nilaparvata lugens(St?l)is one of the most serious pests in rice production and high fecundity is the basis of the outbreak of ***,using the genome-wide ChIP-seq of NlFoxO in BPH,we found that NlFoxO binds to the promoters of ribosomal protein S6 kinase(NlS6K)and serine/threonine-protein kinase mTOR(NlTOR)and increases their expression *** also found that NlFoxO directly binds to the exon of vitellogenin(NlVg)and has a specific inhibitory effect on its *** addition,the number of eggs laid and their hatching rate decreased significantly after injection of NlFoxO double-stranded RNA into BPH *** findings provide direct evidence that FoxO modulates insect fecundity through binding to the promoters of NlS6K,NlTOR and the exon of NlVg and affecting their gene expression in the Vg network.

关键词： FoxO ChIP-seq Nilaparvata lugens fecundity vitellogenin(Vg) bind

Population density modulates insect progenitive plasticity through the regulation of dopamine biosynthesis

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Insect Science 2022年第6期29卷 1773-1789页

作者： Kai Liu Longyu Yuan Lei Yue Weiwen Chen kui kang Jun Lv Wenqing Zhang Rui Pang State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Microbial Safety and Health State Key Laboratory of Applied Microbiology Southern ChinaInstitute of MicrobiologyGuangdong Academy of SciencesGuangzhouChina Innovative Institute for Plant Health College of Agriculture and BiologyZhongkai Universityof Agriculture and EngineeringGuangzhouChina

Insect fecundity is a quantitative phenotype strongly affected by genotypes and the ***,interactions between genotypes and environmental factors in modulating insect fecundity remain largely *** study investigated the im-pact of population density on the fecundity of Nilaparvata lugens(brown planthopper;BPH)carrying homozygous high-(HFG)or low-(LFG)fecundity homozygous *** low population densities,the fecundity and population growth rate of both genotypes showed similar increasing trends across generations,while the trends between HFG and LFG under high population densities were *** a combination of temporal analysis and weighted gene co-expression network analyses on RNA-seq data of HFG and LFG under low and high population densities in the lst,3rd,and 5th gener-ations,we identified 2 gene modules that were associated with these density-dependent progenitive *** pathways related to the neural system were simultaneously enriched by the 2 gene ***,Nlpale,which encodes a tyrosine hydroxy-lase,was identified as a key *** RNA interference of this gene and manipulation of its downstream product dopamine significantly affected the basic and density-dependent progenitive phenotypes of *** findings indicated that dopamine biosynthesis is the key regulatory factor that determines fecundity in response to density changes in dif-ferent BPH ***,this study provides insights into the interaction of a typical environmental factor and insect genotype during the process of population regulation.

关键词： dopamine biosynthesis fecundity genotype Nilaparvata lugens popula-tiondensity

An Enzymatic Reaction Modulated Fluorescence-on Omethoate Biosensor Based on Fe_(3)O_(4)@GO and Copper Nanoparticles

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Journal of Analysis and Testing 2022年第1期6卷 58-66页

作者： Hui Zhang Wu-kui kang Ying Wang Xiu-Zhong Wang Li-Hua Lu College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdao 266109China

The employing of organophosphorus pesticides(OPs),especially,the abusing of OPs,leads to residue accumulation,which causes immense effect to human health and ***,it is an urgent task to develop highly sensitive OPs’detection platforms.A novel enzyme modulated fluorescence-on sensor for sensitive detection of omethoate was successfully constructed,using AT-rich double-stranded DNA(dsDNA)templated copper nanoparticles(CuNPs)as the fluorescent signal and covalently combined magnetic Fe_(3)O_(4)and graphene oxide(GO)as the single-stranded DNA(ssDNA)*** assay just associates acetylcholinesterase(AChE),one strand of dsDNA,acetylcholinein(ATCh),Fe_(3)O_(4)@GO and ascorbic acid(AA)/Cu^(2+).T6-1 and T6-2 are two strands single-stranded DNA(ssDNA)with the AT-rich sequence and continuous T bases at their two ends,*** the aid of Hg^(^(2+)),they can hybridize into a blunt-ended dsDNA with one AT-rich end and one T-Hg^(^(2+))-T base pairs *** omethoate exists,it can inhibit AChE from transducing acetylcholinein(ATCh)into thiocholine(TCh).So,no TCh can snatch Hg^(^(2+))from T-Hg^(^(2+))-T base pairs in blunt-ended T6-1/T6-2.T6-1/T6-2 still keeps its integrity in blunt-ended dsDNA configuration,and hence the subsequently added Fe_(3)O_(4)@GO cannot absorb *** remained blunt-ended T6-1/T6-2 in supernatant can act as the template of CuNPs to produce strong *** developed detection offers a signal-on omethoate detection,which can sensitively detect omethoate in the linear range of 5-200 nmol/L with a detection limit of 2.48 nmol/*** importantly,it can detect omethoate in food and environmental samples,demonstrating high potential in real sample detection.

关键词： Fe_(3)O_(4)@GO AT-rich DNA Copper nanoparticles Omethoate Fluorescence

Ligand dose-dependent activation of signaling pathways through the gustatory receptor NlGr11 linked to feeding efficacy in Nilaparvata lugens

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Insect Science 2023年第5期30卷 1420-1430页

作者： Wei-Wen Chen Kai Lin Jun Lv Qin Su Meng-Yi Zhang kui kang Wen-Qing Zhang State Key Laboratory of Biocontrol and School of Life Sciences Sun Yat-sen UniversityGuangzhouChina College of Biology and Agriculture Zunyi Normal UniversityZunyiGuizhou ProvinceChina

Insects often face both conditions with sufficient nutrients and conditions of undernutrition in the *** gustatory receptors,insects sense nutrients and regulate their physiological functions such as feeding and ***,it remains unclear whether signaling pathways activated by gustatory receptors depend on the concentration of nutrients and whether the difference in signaling pathways directly affects insects’physiological ***,we found that a sugar gustatory receptor,NlGr11,from the brown planthopper(BPH),Nilaparvata lugens,activated G protein-coupled signaling and ionotropic pathways when bound to high galactose *** subsequently demonstrated longer feeding times,feeding loads,and higher vitellogenin(NlVg)expression than BPHs exposed to high galactose concentrations,which only activated the ionotropic *** the first time,our findings link plant nutrient conditions,signaling pathways activated by nutrients,and their gustatory receptors,and nutrient dose-dependent feeding efficacy and vitellogenin(Vg)expression in an *** will help us to better understand the molecular mechanism for insect feeding strategies on plants at different stages of nutritional conditions.

关键词： feeding efficacy galactose G protein-coupled signaling pathway insect gustatory receptor ionotropic pathway

CROSSING ANASTOMOSIS OF NERVE BUNDLES NEAR INNERVATED ORGANS TO TREAT IRREPARABLE NERVE INJURIES

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Chinese Medical Sciences Journal 2006年第2期21卷 131-134页

作者： Zheng-da Kuang Xin-yu Zhang Jian-xiang Yao Meng-kui kang He Li Jia-zheng Wang Department of Orthopedics General Hospital of Chinese People＇s Armed Police Forces Beijing 100039 People＇s Liberation Army Medical Academy of Sciences Beijing 100039

Objective To study the therapeutical effects of crossing anastomosis of nerve on the peripheral and central nerve injuries. Methods Twelve kinds of central and peripheral nerve disorders and their complications were treated with 11 kinds of crossing anastomosis of nerve bundles near the innervated organs. After nerve injury and repair, somatosensory evoked potentials （SEPs） and horseradish peroxidase （HRP） retrograde tracing studies were used to investigate the rabbit＇s nerve function and morphology. Resets The ulcers of all patients healed. Sensation, voluntary movement, and joint function recovered. Four weeks after the anastomosis of distal stump of radialis superficialis nerve and median nerve, pain sensation regained and SEPs appeared. HRP retrograde tracing studies demonstrated sensory nerve ending of medial nerve formed new connection with the body of neuron. Conclusion Crossing anastomosis of nerve is an effective method to treat peripheral and central nerve injuries.

关键词： anastomosis of nerve nerve injury

基于映射-归约模型的SVM可扩展硬件实现架构

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电路与系统学报 2011年第5期16卷 90-94,89页

作者：曹葵康沈海斌浙江大学超大规模集成电路设计研究所浙江杭州310027

为了提高支持向量机(SVM)在嵌入环境中的适用性,提出了一种用于SVM训练和分类的可扩展硬件架构,并基于FPGA平台测试了其性能。基于映射-归约(MapReduce)模型分析提取出SVM算法中的并行性,并进一步映射至多个并行处理单元。实验表明,该... 详细信息

为了提高支持向量机(SVM)在嵌入环境中的适用性,提出了一种用于SVM训练和分类的可扩展硬件架构,并基于FPGA平台测试了其性能。基于映射-归约(MapReduce)模型分析提取出SVM算法中的并行性,并进一步映射至多个并行处理单元。实验表明,该架构可基于定点运算单元有效地完成SVM训练和分类,并具有良好的可扩展性。

关键词：支持向量机并行计算映射-归约现场可编程门阵列