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作者：杨锐鸿（Marvin Yui Hung yeung）清华大学

学位级别：硕士

生物滤塔是一种高效的挥发性有机物（VOCs）处理技术,但对于难溶于水、难生物降解的VOCs去除率低,且存在生物量累积堵塞填料层的问题。以往研究表明,电化学技术在难生物降解污染物去除及微生物增殖抑制方面均有一定潜力。本研究建立了... 详细信息

生物滤塔是一种高效的挥发性有机物（VOCs）处理技术,但对于难溶于水、难生物降解的VOCs去除率低,且存在生物量累积堵塞填料层的问题。以往研究表明,电化学技术在难生物降解污染物去除及微生物增殖抑制方面均有一定潜力。本研究建立了一套电化学强化的生物滤塔系统（Electrochemical Biofilter,EBF）,研究了电流强度、营养盐成分及VOCs种类对EBF系统运行性能及微生物群落结构和代谢特性的影响,同时通过检测模拟EBF系统运行条件下的氧化性物质,探讨了EBF的强化机理。关于电流强度对EBF性能影响的研究表明,50 mA电流强度对甲苯去除的提升最为明显,平均去除率达到66.5%,无电流时的甲苯去除率仅为58.5%。同时,50mA条件下,滤塔生物量累积速率较无电流时降低48%,塔中微生物对碳源的代谢活性也明显升高。微生物群落结构研究结果表明,通电后Rhodococcus菌属丰度降低,但去除性能提高。推测电化学反应不通过影响微生物群落结构而直接参与了甲苯降解。关于营养盐对EBF系统性能影响的研究表明,使用氯化铵作为氮源时EBF对甲苯去除的强化效果最为明显,50mA电流条件下EBF的甲苯去除能力比单一生物滤塔增加9.6%,生物量增殖的抑制率为62%,同时微生物碳源代谢活性显著提高。微生物群落结构研究表明,通电后在以铵盐作为氮源的EBF中硝化细菌Nitrosomonas丰度有所下降,降低了VOCs降解菌竞争氮源的压力。EBF处理四种单一VOCs气体的结果表明,通电后EBF对正己烷,苯乙烯,二氯甲烷及二异丁烯的去除能力相对增长约30.7%,48.7%,35.7&及-10.6%,生物量增长抑制率为31.9%、62.2%、21.2%和-2.1%。考察了电化学模拟系统中的氧化物及超氧化物歧化酶。结果表明,在50 mA电流强度下,在氯化铵电解环境中余氯浓度于20 min达到0.24 mg/m,于硫酸铵和硝酸钠中浓度低于0.05 mg/m。羟基自由基及过氧化氢在三种盐中使用1700mA电流环境中浓度各高于1.5 mg/l和2 mg/l。超氧化物歧化酶抑制率在50 mA的各样本中均高于0.6%。微生物群落研究结果表明,EBF去除甲苯时在门的分类水平上大致有4个优势菌门,分别为Proteobacteria,Actinobacteria,Firmicutes及Bacteroidetes。其中Proteobacteria丰度在加电后下降,而Firmicutes及Bacteroidetes丰度在加电后上升。属水平结果表明,Rhodococcus菌属受通电影响较明显,通电后丰度下降;Halomonas、Nitrobacter及B-42菌属通电后丰度相对较稳定,通电前后,受通电的影响较小。

关键词：挥发性有机物电化学生物滤塔微生物群落

香港新生儿卡介苗接种队列实施卡介苗复种规程的效果

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国际结核病与肺部疾病杂志 2002年第1期 2-7页

作者： C.C.leung C.M.Tam S.l.Chan M.Chan-yeung C.k.Chan k.C.Chang 王海东 TB and Chest Service Department of Health Department of Medicine The University of Hong Kong Hong Kong SAR China

背景:香港对结核菌素阴性的在校儿童进行卡介苗复种成为常规。目的:应用结核菌素皮试评定卡介苗复种的效果。方法:通过发生活动性结核病的登记报告随访一钮在出生时接种卡介苗的303692名儿童。以香港卫生局的接种统计,评估小学参加卡... 详细信息

背景:香港对结核菌素阴性的在校儿童进行卡介苗复种成为常规。目的:应用结核菌素皮试评定卡介苗复种的效果。方法:通过发生活动性结核病的登记报告随访一钮在出生时接种卡介苗的303692名儿童。以香港卫生局的接种统计,评估小学参加卡介苗复种规程的队列百分比。通过接种卡和门诊记录来确定儿童的卡介苗复种史。结果:队列中参加卡介苗复种规程的有85.2％;参与者中的79.7％结核菌素阴性并复种;343人在11岁后发展为结核病;参与者除41人离开外,其余302人均在规程中。参与者和未参与者的结核病发病率分别为16.5和12.9每100000人年(RR 1.28,95％CI 0.92-1.77)。在参与者中,结核菌素阴性/卡介苗复种组和结核菌素阳性/未复种组的结核病发病率分别是12.5和32.0每100000人年(RR 0.39,95％CI 0.31-0.49)。结论:本研究未能证明学校卡介苗复种规程的参与者与未参与者间在结核病发病率上有显著性差异。结核菌素阳性组结核病危险的增加不支持使用结核菌素试验来检测新生儿卡介苗接种产生的免疫。

关键词：卡介苗结核菌素复种效果

An update on hormonal regulation of early embryo-fallopian tube interactions: insights for improvements of assisted reproductive technology outcomes

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Reproductive and Developmental Medicine 2024年第1期8卷 39-49页

作者： Suranga P.kodithuwakku Ernest H.Y.Ng William S.B.yeung kai-Fai lee Department of Obstetrics and Gynaecology LKS Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Department of Animal Science Faculty of AgricultureUniversity of PeradeniyaPeradeniya 20400Sri Lanka Shenzhen key laboratory of Fertility Regulation.The University of Hong kong-Shenzhen Hospital Shenzhen 518053China

Human fallopian tubes(FTs)play important roles in the whole reproductive *** are the site for the final maturation of gametes,fertilization,transport of preimplantation embryos,and early embryonic development before entering the *** these functions are critical in the establishment of a successful *** the embryo is in transit through the FT,the FT microenvironment changes through endocrine,paracrine,autocrine,and juxtacrine mechanisms which involve various hormones and bioactive substances mainly from the neighboring ***,the interactions between the embryo and the FT may be the earliest embryo-maternal communications that are essential for the establishment of *** review critically discusses the evidence of various hormonal and bioactive mediated control of the microenvironment in the FTs during the earliest embryo-maternal ***,we highlight existing gaps in the knowledge and the importance of exploring more of the physiology of FTs which will hold the key to producing high-quality embryos for patients seekingin vitro fertilization(IVF)treatments.

关键词： Fallopian tube Embryo Maternal-embryo communication Hormonal control

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用于检测中文字符识别错误的多分类器方法

图象识别与自动化 2005年第2期 16-30页

作者： k. - Y. Hung R. W. P. luk D.S. yeung k. F. l. Chung W. Shu 范生淼（译）

在很多领域中，识别错误的检测是一项重要的工作，比如进一步提高检测识别的性能，节约阅读校对和修改、编辑需要的人工劳动，以及在构造数字图书馆时为检索分配合适的权重等。本文提出一种用于多分类器检测识别错误的新方法。采用单一... 详细信息

在很多领域中，识别错误的检测是一项重要的工作，比如进一步提高检测识别的性能，节约阅读校对和修改、编辑需要的人工劳动，以及在构造数字图书馆时为检索分配合适的权重等。本文提出一种用于多分类器检测识别错误的新方法。采用单一分类器和单一门限的检测方法，并不能提高检测识别错误的能力，因此需要使用多分类器。虽然一个分类器无法提高检测识别错误的能力，但它提供了比较的基线，相关的错误检测其有用特征的学习表明了可以提供三种不同的情况。在每一种情况中，多分类器分别分配一个分类器检测是否有错误，并且考虑每种情况的额外特征。实验结果表明，识别错误的回现率为70％-80％，识别错误的检测精确率为80％-90％，比使用单一分类器和单一门限检测方法具有更好的效果，能节省75％的人工劳动。

关键词：字符识别错误检测模式识别和语言模型

亲和毛细管电泳激光诱导荧光法测定牛血清白蛋白与其单克隆抗体的结合常数

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高等学校化学学报 1999年第10期20卷 1551-1553页

作者：王清刚罗国安杨树标清华大学化学系北京100084 香港大学妇产科系

The binding constant( k b) between fluorescein isothiocyanate labeled bovine serum albumin(FITC BSA) and its monoclonal antibody was determined using the ligand separation mode of affinity capillary electrophoresis(ACE) with laser induced fluorescence detection(lIF). Free FITC BSA and the complexes were separated by capillary electrophoresis in 7 min after incubation. The running buffer was 100 mmol/l HEPES at pH 7 4, and separation voltage was 30 kV. The k b was determined to be 4 7×10 8 l/mol using Scatchard plotting method. The influences of incubation time and electrophoresis conditions on determination were also studied.

关键词：单克隆抗体 BSA ACE 蛋白质结合常数

Stromal cell decidualization and embryo implantation: a vulnerable step leading to successful pregnancy

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Reproductive and Developmental Medicine 2024年第2期8卷 101-110页

作者： luhan Jiang Sudini R.Fernando Suranga P.kodithuwakku Dandan Cao William S.B.yeung kai-Fai lee Department of Obstetrics and Gynaecology School of Clinical MedicineLKS Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Department of Animal Science Faculty of Animal Science&Export AgricultureUva Wellassa UniversityBadulla 50000Sri Lanka Department of Animal Science Faculty of AgricultureUniversity of PeradeniyaPeradeniya 20400Sri Lanka Shenzhen key laboratory of Fertility Regulation.The University of Hong kong-Shenzhen Hospital Shenzhen 518053China

Endometrial stromal cell decidualization is a crucial step in endometrial remodeling during *** is controlled by orchestrated ovarian hormones,followed by the activation of various downstream signaling *** evidence has shown multiple functions of decidualized endometrial stromal cells during embryo implantation,including tissue remodeling,antioxidative stress,angiogenesis,and immune *** distinct secretomes of decidualized stromal cells also reveal their intensive interactions with epithelial,endothelial,and immune ***,aberrant decidualization leads to pregnancy failures,such as recurrent pregnancy loss and repeated implantation *** review aimed to provide an overview of the molecular mechanisms underlying the divergent functions of decidualized endometrial stromal cells and their potential clinical ***,the use of single-cell RNA sequencing data further enhances our understanding of these biological *** review discusses decidualization-related signaling pathways that serve as potential therapeutic targets for treating implantation failure in in vitro fertilization and provides novel approaches to investigate the underlying causes of female infertility.

关键词： Endometrial stromal cell Decidualization Embryo implantation Recurrent pregnancy loss Repeated implantation failure

Use of biological and chemical molecules in regulating embryo implantation and endometrial receptivity

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Reproductive and Developmental Medicine 2022年第4期6卷 234-242页

作者： Xian Chen Shu-Ya Sun Ernest Hung-Yu Ng Raymond Hang-Wun li William Shu-Biu yeung kai-Fai lee Department of Obstetrics and Gynaecology LKS Faculty of Medicinethe University of Hong KongHong Kong SARChina Shenzhen key laboratory of Fertility Regulation the University of Hong Kong-Shenzhen HospitalShenzhen 518053China

This review summarizes the drugs and chemicals that may modulate embryo ***-hormonal molecules,including aspirin,improved endometrial blood flow,while low molecular weight heparin,vitamin E,sildenafil,and atosiban modulated the expression of endometrial *** factors,including human chorionic gonadotropin and growth hormones,can regulate the expression of endometrial receptivity *** immunomodulatory molecules,including granulocyte colony-stimulating factor,peripheral blood mononuclear cells,autologous platelet-rich plasma,and intralipid and intravenous immunoglobulins,may improve implantation rate by modulating endometrial immune *** extracts of the Chinese herbs Paeonia lactiflora and Perilla frutescens increased the expression of leukemia inhibitory factors in endometrial epithelial ***,the use of the commercially available library of Pharmacologically Active Compounds with a high-throughput screening method has provided an approach to screen for compounds that may potentially enhance or suppress embryo *** these biomedical findings translate into clinical effects that enhance or suppress embryo implantation requires further investigation.

关键词： Embryo implantation Endometrial receptivity fertilization Repeated implantation failure Birth control Emergency contraception

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澳门智慧城市建设与降低环境噪声的进展

声学技术 2020年第5期39卷 622-626页

作者：梁浩贤杜伟明钟伟梁洪汉杰杨国良姚景光澳门声学学会中国澳门澳门理工学院中国澳门

澳门在过去十年中迅速发展。同时,基础设施项目数量大幅增加,包括新区的发展,机场航站楼的延伸,轻轨交通,路凼的新道路,以及废物管理和污水处理设施。其中一些项目还处于规划阶段,一些正在设计中,一些正在建设中,另一些正在运营中。为... 详细信息

澳门在过去十年中迅速发展。同时,基础设施项目数量大幅增加,包括新区的发展,机场航站楼的延伸,轻轨交通,路凼的新道路,以及废物管理和污水处理设施。其中一些项目还处于规划阶段,一些正在设计中,一些正在建设中,另一些正在运营中。为了整合各种各样的基础设施项目,改善民生,澳门政府已着手实施建设智慧城市,智慧医疗,智能城市管理,智慧旅游等的举措。文章介绍了澳门智慧城市的建设与降低环境噪声的进展。

关键词：智慧城市噪音策划运输中国澳门

Microtubule Structure and Male Sterility in a Gene-Cytoplasmic Male Sterile line of Rice, Zhen Shan 97A

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Acta Botanica Sinica 2003年第2期45卷 183-192页

作者：叶秀麟杨子德徐是雄梁承邺香港大学植物系加拿大卡加里大学生物系中国科学院华南植物研究所广州510650

Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice ( Oryza saliva l.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 of a Mine hybrid rice production system. In the restorer line, Ce64, the developing microsporocytes have dense cytoplasm and a distinct set of circumferential microtubules around the nucleus. Successive cytokinesis results in the formation of tetrads. The microtubules within the cells of tetrads and microspores radiate from the surface of the nucleus towards the outer edge of the cytoplasm. Subsequent pollen development is normal. During the course of microspore formation tubulin speckles can be found in the cytoplasm. The general pattern of development and microtubule organization in the maintainer lined Zhen Shan 97B, is similar to Ce64, except that a few more tubulin speckles appear during microspore formation. In the case of the mate sterile line, Zhen Shan 97A, a number of abnormalities can be discerned during early microsporogenesis. These include vacuoles forming within the developing microsporocyte and faintly stained microtubules with no defined distribution pattern. Prominent tubulin speckles are common within the cytoplasm. For those microsporocytes that undergo meiosis, no defined organizational patterns of microtubules can be found within the tetrad. All microspores abort soon after. Abnormalities and defects in microtubule organization observed in Zhen Shan 97A showed that complex interactions between the cytoplasm and the nucleus began at very early stage of microsporocyte development.

关键词： Oryza saliva cytoplasmic male sterility microtubules microsporocyte vacuole tubulin speckles