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A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

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National Science Review 2022年第8期9卷 100-109页

作者： Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang omar A.Al-Hartomy Swelm Wageh Abdullah G.Al-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang Shenzhen Engineering Laboratory of Phosphorene and Optoelectronics International Collaborative Laboratory of 2D Materials for Optoelectronics Science and Technology of Ministry of Education 3. Shenzhen Institute of Translational Medicine 4. Department of Otolaryngology Shenzhen Second People’s Hospital 5. the First Affiliated Hospital 6. Institute of Microscale Optoelectronics Shenzhen University 7. Shenzhen International Institute for Biomedical Research 8. Shenzhen Han’s Tech Limited Company 9. Shenzhen Metasensing Tech Limited Company Department of Physics Faculty of Science King Abdulaziz University Research Center for Advanced Materials Science (RCAMS) King Khalid University Department of Chemistry College of Science King Khalid University Zhongmin (Shenzhen) Intelligent Ecology Co.Ltd Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Department of Laboratory Medicine Shenzhen Children’s Hospital Optoelectronics Research Center School of Science Minzu University of China NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Research Unit of Adaptive Evolution and Control of Emerging Viruses Chinese Academy of Medical Sciences Institute of Pathogenic Organism Shenzhen Center for Disease Control and Prevention Respiratory Department Shenzhen Children’s Hospital Institute of Pediatrics Shenzhen Children’s Hospital

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-Co V-2 variants,including Omicron, are required to be identified as soon as the samples are identified as ‘positive’.Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance(SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat(CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification,within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-Co V-2, and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron) and BA.1(a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.

关键词： surface plasmon resonance CRISPR SARS-CoV-2 Omicron variant

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硫胺素及其磷酸酯与沙特阿拉伯人心血管代谢危险因素的相关性分析

硫胺素及其磷酸酯与沙特阿拉伯人心血管代谢危险因素的相关性分析

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作者： Nasser M Al-Daghri omar S Al-Attas khalid M Alkharfy Majed S Alokail Sherif H Abd-Alrahman Shaun Sabico 刘庆春胡南摘

目前已表明硫胺素缺乏与几种胰岛素抵抗并发症有关。在本研究中,我们根据修订后的《国家胆固醇教育计划-成人治疗小组III》,随机招募沙特成年志愿者(166名男性和70名女性),并筛选出患有代谢综合征(MetS)的人员。用HPLC分析探讨循环硫胺... 详细信息

目前已表明硫胺素缺乏与几种胰岛素抵抗并发症有关。在本研究中,我们根据修订后的《国家胆固醇教育计划-成人治疗小组III》,随机招募沙特成年志愿者(166名男性和70名女性),并筛选出患有代谢综合征(MetS)的人员。用HPLC分析探讨循环硫胺素水平与心血管代谢相关参数的关系。

关键词：阿拉伯代谢综合征硫胺素

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The Impact of Electronic Cigarettes (e-Cigs) Smoking Habit on Periodontal Status and Salivary pH among Some Passive e-Cigs Smokers Referred to the College of Dentistry Clinics, King khalid University

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International Journal of Clinical Medicine 2022年第11期13卷 531-547页

作者： Mohammed M. A. Abdullah Al-Abdaly Ibrahim Abdullah Ahmed Asiri Abdulhamid Ahmed Faya omar Abdulaziz Abdullah Aldhaban Abdulaziz Saeed Ali AlJuman Periodontics Periodontics and Community Dental Sciences College of Dentistry King Khalid University Abha KSA College of Dentistry King Khalid University Abha KSA

Background: Electronic cigarette (e-cigs) smoking is substitutional to traditional cigarette smoking to reduce the dangerous combustion of products. Moreover, passive smoking is involuntarily tobacco smoking due to the exposure to cigarette or tobacco smoke among non-smokers and due to there being little knowledge about the impact of passive e-cigs smoking on periodontal status and salivary pH. Therefore, the present study aimed to evaluate the effect of e-cigs smoking habit on periodontal tissue and salivary pH among some passive e-cigs smokers referred to the college of dentistry clinics, King khalid University. Material and Methods: Ninety male participants who were referred to the college of dentistry clinics at King khalid University were included in the study. Age, gender, e-cigs smoking, and general health were recorded. The participants were divided into three equal groups (n = 30) as follows: Group I (Non-passive e-cigs smokers and non-smokers) as the control group, Group II (e-cigs users), and Group III (Passive e-cigs smokers). Salivary pH, plaque control record (PCR), gingival bleeding index (GBI), clinical attachment loss (CAL), percentage of radiographic bone loss (% RBL), periodontal pocket depth (PPD), more than 5 missing teeth due to periodontal diseases (>5 MTDP), tooth mobility (TM), furcation involvement (FI), Bite collapse (BC), and less than 20 remaining teeth (10 Opposing pairs) (L20RT) as well as HbA1c were recorded. ANOVA test was used to the comparison between Groups I, II, and III in the participants’ ages and periodontitis staging clinical findings. The mean of participants’ age groups, the mean of salivary pH values of study groups, and the periodontitis staging complexity and HbA1c were compared between groups with the ANOVA test, Tukey’s test, and the chi-square test. P-value was recorded, and less than 0.5 was considered a statistically significant difference (p Results: The e-cigs users group revealed higher means of PCR, GBI %RBL va

关键词： Electronic Smoking Habit King khalid University Passive e-Cigs Smokers Periodontal Status Salivary pH

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