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在片S参数计量比对结果浅析

计量学报 2024年第9期45卷 1401-1406页

作者：刘晨高岭栾鹏陈婷黄英龙李艳奎金诚邹喜跃陆景陈科元中国电子科技集团公司第十三研究所河北石家庄050051 北京无线电计量测试研究所北京100854 中国电子技术标准化研究院北京100176 中国科学院微电子研究所北京100029 中国电子科技集团公司第十四研究所江苏南京210013 湖南时变通讯科技有限公司湖南湘潭411104 华美博科技(北京)有限公司北京100097 河北雄安太芯电子科技有限公司河北保定071799

中国电子科技集团公司第十三研究所作为主导实验室开展了在片S参数计量比对工作,对参比实验室提交的在片S参数测量结果进行了汇总分析,并用E_n值对各参比实验室测量结果进行了评价。通过在片S参数计量比对,确保了量值传递的准确、可靠,... 详细信息

中国电子科技集团公司第十三研究所作为主导实验室开展了在片S参数计量比对工作,对参比实验室提交的在片S参数测量结果进行了汇总分析,并用E_n值对各参比实验室测量结果进行了评价。通过在片S参数计量比对,确保了量值传递的准确、可靠,特别是对在片S参数测量不确定度的主要来源统一了认识。同时也为业内提供了在片S参数测量一致性的比较平台。

关键词：无线电计量在片S参数计量比对矢量网络分析仪

Identification and Characterization of a Distinct Strain of Beak and Feather Disease Virus in Southeast China

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Virologica Sinica 2020年第1期35卷 43-51页

作者： Yanmei Ma Xiaoyong chen keyuan chen Xiancheng Zeng Shili Yang Wei Chang Yao Tang Xiaoli chen Song Wang Ji-Long chen Fujian Agriculture and Forestry University Fuzhou 350002China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Fuzhou Zoo Fuzhou 350012China

Beak and feather disease virus(BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds. In March 2017, an epidemic of psittacine beak and feather disease(PBFD) struck a farm in Fuzhou in the Fujian Province of southeast China, resulting in the death of 51 parrots. In this study, the disease was diagnosed and the pathogen was identified by PCR and whole genome sequencing. A distinct BFDV strain was identified and named as the FZ *** BFDV strain caused severe disease symptoms and pathological changes characteristic of typical PBFD in parrots, for example, loss of feathers and deformities of the beak and claws, and severe pathological changes in multiple organs of the infected birds. Phylogenetic analysis showed that the FZ strain was more closely related to the strain circulating in New Caledonia than the strains previously reported in China. Nucleotide homology between the FZ strain and other 43 strains of BFDV ranged from 80.0% to 92.0%. Blind passage experiment showed that this strain had limited replication capability in SPF Chicken Embryos and DF-1 Cells. Furthermore, the capsid(Cap) gene of this FZ strain was cloned into the p GEX-4 T-1 expression vector to prepare the polyclonal anti-Cap antibody. Western blotting analysis using the anti-Cap antibody further confirmed that the diseased parrots were infected with BFDV. In this study, a PBFD and its pathogen was identified for the first time in Fujian Province of China, suggesting that future surveillance of BFDV should be performed.

关键词： Beak and feather disease virus(BFDV) Parrot FZ strain Capsid protein(Cap) Phylogenetic analysis

牛病毒性腹泻病毒E^(rns)-ELISA抗体检测试剂盒的制备

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中国兽医杂志 2024年第7期60卷 65-70页

作者：王静陈柯源王胜华丁成志杨光辉刘一尹金花王九峰中国农业大学动物医学院北京海淀100193 中国动物疾病预防控制中心北京大兴102618 塔里木大学动物科学与技术学院新疆阿拉尔843300

为建立快速检测牛病毒性腹泻病毒(BVDV)的血清学方法,本试验以BVDV NADL毒株结构蛋白E^(rns)作为包被抗原,优化反应条件并组装BVDV酶联免疫吸附试验(ELISA)抗体检测试剂盒,通过重复性试验、灵敏性试验、符合率试验和保存期试验验证该试... 详细信息

为建立快速检测牛病毒性腹泻病毒(BVDV)的血清学方法,本试验以BVDV NADL毒株结构蛋白E^(rns)作为包被抗原,优化反应条件并组装BVDV酶联免疫吸附试验(ELISA)抗体检测试剂盒,通过重复性试验、灵敏性试验、符合率试验和保存期试验验证该试剂盒的准确性、灵敏性和稳定性。结果显示,抗原包被浓度为4μg/mL,1%酪蛋白溶液37℃封闭45 min,被检血清以1∶400稀释孵育30 min,兔抗牛HRP标记二抗以1∶20000稀释孵育30 min,TMB底物反应5 min时,ELISA的反应背景下降,区分度最好。优化后方法的批内重复性试验和批间重复性试验的变异系数均在10%以内,表明该试剂盒具有较好的稳定性;灵敏性试验结果显示,当阳性血清稀释度达到1∶6400时仍可以检测为阳性,说明该试剂盒灵敏度较高;该试剂盒与美国爱德士生物科技公司(IDEXX)BVDV总抗体检测试剂盒共同检测466份临床血清样品,两者总符合率为89.1%;保存期试验结果显示,第4个月时该试剂盒仍能检测到阳性血清,说明稳定性较好。综上表明,本试验利用E^(rns)蛋白建立的BVDV间接ELISA抗体检测试剂盒灵敏度高且重复性好,可为BVDV抗体检测和流行性调查提供新的方法。

关键词：牛病毒性腹泻病毒 E^(rns)蛋白抗体间接ELISA

Time-Varying Mesh Stiffness Calculation and Dynamic Modeling of Spiral Bevel Gear with Spalling Defects

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Journal of Dynamics, Monitoring and Diagnostics 2024年第2期3卷 143-155页

作者： keyuan Li Baijie Qiao Heng Fang Xiuyue Yang Xuefeng chen School of Mechanical Engineering Xi’an Jiaotong UniversityXi’anPR China National Key Lab of Aerospace Power System and Plasma Technology Xi’an Jiaotong UniversityXi’anPR China

Time-varying mesh stiffness(TVMS)is a vital internal excitation source for the spiral bevel gear(SBG)transmission *** defect often causes decrease in gear mesh stiffness and changes the dynamic characteristics of the gear system,which further increases noise and *** paper aims to calculate the TVMS and establish dynamic model of SBG with spalling *** this study,a novel analytical model based on slice method is proposed to calculate the TVMS of SBG considering spalling ***,the influence of spalling defect on the TVMS is studied through a numerical simulation,and the proposed analytical model is verified by a finite element ***,an 8-degrees-of-freedom dynamic model is established for SBG transmission *** the spalling defect into TVMS,the dynamic responses of spalled SBG are *** numerical results indicate that spalling defect would cause periodic impact in time ***,an experiment is designed to verify the proposed dynamic *** experimental results show that the spalling defect makes the response characterized by periodic impact with the rotating frequency of spalled pinion.

关键词： dynamic modeling slice method spalling spiral bevel gear time-varying mesh stiffness(TVMS)

STAT3-NAV2 axis as a new therapeutic target for rheumatoid arthritis via activating SSH1L/Cofilin-1 signaling pathway

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2690-2693页

作者： Ran Wang Jianghong Cai keyuan chen Menglin Zhu Zhaoyi Li Hua Liu Tiantian Liu Jianchun Mao Qian Ding Yi Zhun Zhu State Key Laboratory of Quality Research in Chinese Medicine School of PharmacyMacao University of Science and TechnologyAvenida Wai LongTaipaMacaoChina School of Pharmacy Minzu University of ChinaBeijing 100081China Department of Pharmacy Aerospace Center HospitalBeijing 100049China Department of Rheumatology Longhua Hospital of Shanghai University of Traditional Chinese MedicineShanghai 200032China Shanghai Key Laboratory of Bioactive Small Molecules Department of PharmacologySchool of PharmacyFudan UniversityShanghai 201203China

Dear Editor,Rheumatoid arthritis(RA),which is the most common inflammatory arthritis disease,as well as a kind of most prevalent autoimmune disease,affects 1–3%population all over the world.1 Currently available treatments can reduce the disability rate of patients with RA to some extent,but they cannot completely block the inflammatory joint destruction and relieve the pain that comes with *** previous study found that Neuron Navigator 2(NAV2)increased significantly in RA and was regulated by transcription factor E2F1.2 However,it is still unclear why NAV2 is elevated at the genetic level in RA fibroblast-like synoviocytes(FLS).Additionally,RA’s exact pathological mechanisms and therapeutic targets are also not fully ***,an indepth study of the pathogenesis of RA is urgently needed.

关键词： therapeutic STAT3 elevated

Aloe emodin promotes mucosal healing by modifying the differentiation fate of enteroendocrine cells via regulating cellular free fatty acid sensitivity

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Acta Pharmaceutica Sinica B 2024年第9期14卷 3964-3982页

作者： Weilian Bao Jiaren Lyu Guize Feng Linfeng Guo Dian Zhao keyuan You Yang Liu Haidong Li Peng Du Daofeng chen Xiaoyan Shen Department of Pharmacology&the Key Laboratory of Smart Drug Delivery Ministry of Education School of PharmacyFudan UniversityShanghai 201210China Department of Natural Medicine School of PharmacyFudan UniversityShanghai 201210China Department of Colorectal and Anal Surgery Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China Shanghai Colorectal Cancer Research Center Shanghai 200092China

The proper differentiation and reorganization of the intestinal epithelial cell population is critical to mucosal regeneration post *** retaining cells(LRCs)expressing SRY-box transcription factor 9(SOX9)promote epithelial repair by replenishing LGR5 t intestinal stem cells(ISCs).While,LRCs are also considered precursor cells for enteroendocrine cells(EECs)which exacerbate mucosal damage in inflammatory bowel disease(IBD).The factors that determine LRC-EEC differentiation and the effect of intervening in LRC-EEC differentiation on IBD remain *** this study,we investigated the effects of a natural anthraquinone called aloe emodin(derived from the Chinese herb rhubarb)on mucosal healing in IBD *** findings demonstrated that aloe emodin effectively interfered with the differentiation to EECs and preserved a higher number of SOX9t LRCs,thereby promoting mucosal ***,we discovered that aloe emodin acted as an antagonist of free fatty acid receptors(FFAR1),suppressing the FFAR1-mediated Gbg/serine/threonine-protein kinase(AKT)pathway and promoting the translocation of forkhead box protein O1(FOXO1)into the nucleus,ultimately resulting in the intervention of differentiation *** findings reveal the effect of free fatty acid accessibility on EEC differentiation and introduce a strategy for promoting mucosal healing in IBD by regulating the FFAR1/AKT/FOXO1 signaling pathway.

关键词： LRCs ISCs EECs IBD SOX9 Aloe emodin FFAR1 FOXO1

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我国跨座式单轨旅游交通系统的现状与发展

中南大学学报（自然科学版） 2021年第12期52卷 4540-4551页

作者：郭风琪陈柯源顾发根王辉文婷中南大学土木工程学院湖南长沙410075 株洲中车特种装备科技有限公司湖南株洲412004 湖南柏宇建设工程咨询有限公司湖南长沙410017

近年来,一种新型跨座式单轨旅游交通系统快速发展,其具有对地形适应性强、对环境友好、施工简便及造价低等优点,特别适用于景区内和景区间的交通与观光,是对大交通系统的有益补充。本文阐述了系统的构造特点、系列车型及典型工程应用案... 详细信息

近年来,一种新型跨座式单轨旅游交通系统快速发展,其具有对地形适应性强、对环境友好、施工简便及造价低等优点,特别适用于景区内和景区间的交通与观光,是对大交通系统的有益补充。本文阐述了系统的构造特点、系列车型及典型工程应用案例,梳理了其从国外引进到自主研发的发展历程。系统整理了轨道梁动力特性、轨道梁不平顺和运行平稳性与乘坐舒适性等方面的研究现状,介绍了特别为该系统研发的专用支座和轨道梁安装设备。分析了跨座式单轨旅游交通系统现有研究存在的问题,指出该系统缺乏针对性的抗震性能、轨道梁温度场研究,轨道不平顺及设计参数研究也需深入。阐明了系统建设过程中的管理制度、设计施工规范欠缺的现状,探讨了应进一步深入研究的课题,指出了未来多样化、装配化和专业化的发展方向,对我国跨座式单轨旅游交通系统的应用和发展具有一定的参考价值。

关键词：跨座式单轨旅游交通系统动力学指标车桥耦合

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基因型和pH对马铃薯脱毒苗生长的影响

种子 2015年第7期34卷 20-25页

作者：陈科元陈彦云宁夏大学西北土地退化与生态恢复国家重点实验室培育基地银川7500211 宁夏大学生命科学学院银川7500212

以大西洋、克新1号和青薯168的脱毒基础苗为试验材料,采用2因素随机区组设计,研究不同基因型、pH值对马铃薯脱毒基础苗单株鲜重、单株干重、叶片数、叶面积、株高、茎粗、节长、节数、根长和根条数影响,培养14d时,测定各性状指标。结果... 详细信息

以大西洋、克新1号和青薯168的脱毒基础苗为试验材料,采用2因素随机区组设计,研究不同基因型、pH值对马铃薯脱毒基础苗单株鲜重、单株干重、叶片数、叶面积、株高、茎粗、节长、节数、根长和根条数影响,培养14d时,测定各性状指标。结果表明：基因型对10个农艺性状指标影响显著（p〈0.05）;5种pH值除对茎粗、节数、根条数影响不显著外,对其余7个农艺性状指标有显著性影响（p〈0.05）;3个不同基因型和pH值对马铃薯单株鲜重、干重、叶片数、叶面积存在显著性交互作用（p〈0.05）,其余农艺性状受基因型和pH处理交互作用影响不显著。大西洋快繁的适宜pH值为5.6~6.0,克新1号和青薯168快繁的适宜pH值为5.6~6.2,综合农艺性状良好。建议工厂化生产时,将pH值统一调到5.6~6.0,便于生产管理。

关键词：马铃薯基因型品种 pH值生长

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电量传感器高可靠性试验技术

兵工自动化 2015年第12期34卷 26-28页

作者：陈柯源绵阳市维博电子有限责任公司四川绵阳621000

针对电量传感器产品可靠性愈加突出的问题,总结最高效的电量传感器可靠性筛选试验方法。对电子产品的可靠性试验技术及其数学模型进行介绍,重点阐述适合电量传感器的环境应力筛选试验项目,对各试验筛选强度和筛选效率进行探讨,并提出改... 详细信息

针对电量传感器产品可靠性愈加突出的问题,总结最高效的电量传感器可靠性筛选试验方法。对电子产品的可靠性试验技术及其数学模型进行介绍,重点阐述适合电量传感器的环境应力筛选试验项目,对各试验筛选强度和筛选效率进行探讨,并提出改进建议,可为电量传感器应用行业的产品可靠性研究提供参考。

关键词：可靠性筛选度环境应力失效