Identification and Characterization of a Distinct Strain of Beak and Feather Disease Virus in Southeast China

作     者：Yanmei Ma Xiaoyong Chen Keyuan Chen Xiancheng Zeng Shili Yang Wei Chang Yao Tang Xiaoli Chen Song Wang Ji-Long Chen Yanmei Ma;Xiaoyong Chen;Keyuan Chen;Xiancheng Zeng;Shili Yang;Wei Chang;Yao Tang;Xiaoli Chen;Song Wang;Ji-Long Chen

作者机构：Fujian Agriculture and Forestry UniversityFuzhou 350002China CAS Key Laboratory of Pathogenic Microbiology and ImmunologyInstitute of MicrobiologyChinese Academy of Sciences(CAS)Beijing 100101China Fuzhou ZooFuzhou 350012China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第1期

页      面：43-51页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：the National Key Research and Development Program of China(2018YFD0500100,2016YFD0500205) Discipline Development Grant from College of Animal Sciences FAFU(2018DK003) Natural Science Foundation of China(31602046) National Basic Research Program(973)of China(2015CB910502) Program for Fujian University Outstanding Youth Scientific Research,Science and Technology Innovation Special Foundation of Fujian Agriculture and Forestry University(KFA17229A) Collaborative Innovation Center of Animal Health and Food Safety Application Technology in Fujian,Fujian Vocational College of Agriculture(Kla17H01A) 

主　　题：Beak and feather disease virus(BFDV) Parrot FZ strain Capsid protein(Cap) Phylogenetic analysis 

摘      要：Beak and feather disease virus(BFDV) is an infectious agent responsible for feather degeneration and beak deformation in birds. In March 2017, an epidemic of psittacine beak and feather disease(PBFD) struck a farm in Fuzhou in the Fujian Province of southeast China, resulting in the death of 51 parrots. In this study, the disease was diagnosed and the pathogen was identified by PCR and whole genome sequencing. A distinct BFDV strain was identified and named as the FZ *** BFDV strain caused severe disease symptoms and pathological changes characteristic of typical PBFD in parrots, for example, loss of feathers and deformities of the beak and claws, and severe pathological changes in multiple organs of the infected birds. Phylogenetic analysis showed that the FZ strain was more closely related to the strain circulating in New Caledonia than the strains previously reported in China. Nucleotide homology between the FZ strain and other 43 strains of BFDV ranged from 80.0% to 92.0%. Blind passage experiment showed that this strain had limited replication capability in SPF Chicken Embryos and DF-1 Cells. Furthermore, the capsid(Cap) gene of this FZ strain was cloned into the p GEX-4 T-1 expression vector to prepare the polyclonal anti-Cap antibody. Western blotting analysis using the anti-Cap antibody further confirmed that the diseased parrots were infected with BFDV. In this study, a PBFD and its pathogen was identified for the first time in Fujian Province of China, suggesting that future surveillance of BFDV should be performed.