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Progress in the development of techniques based on light scattering for single nanoparticle detection

Science China Chemistry 2011年第8期54卷 1244-1253页

作者： ZHU ShaoBin, WANG Shuo, YANG LingLing, HUANG TianXun & YAN XiaoMei Department of chemical biology, College of Chemistry and chemical Engineering key laboratory for chemical biology of fujian province key laboratory of Analytical Science, Xiamen University, Xiamen 361005, China 1. Department of chemical biology College of Chemistry and Chemical Engineering key laboratory for chemical biology of fujian province key laboratory of Analytical Science Xiamen University Xiamen 361005 China

Nanoparticles have recendy attracted extensive attention in view of their great potential in biomedicine and bioanalytical applications. Single particle detection via light scattering offers a simple and efficient approach for the size, size distribution, and concentration analysis of nanoparticles. In particular, intrinsic heterogeneity or rare events masked by ensemble averaging can be revealed. However, the sixth power dependence of Rayleigh scattering on particle size makes it very challenging to detect individual nanoparficles of small sizes. This article is intended to provide an overview of recent progress in the development of techniques based on light scattering for the detection of single nanoparticles.

关键词： light scattering single particle detection nanoparticles confocal correlation spectroscopy flow cytometry

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Evaluation of non-covalent interaction between Seryl-Histidine dipeptide and cyclophilin A using NMR and molecular modeling

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Science China Chemistry 2010年第9期53卷 1987-1993页

作者： LIU Yan1,SHI YanHong2,LIU XiaoXia1,LIN MingKun1,LIN DongHai1,2 & ZHAO YuFen1,3 1key laboratory for chemical biology of fujian province,Department of chemical biology,Department of Chemistry,College of Chemistry and chemical Engineering,Xiamen University,Xiamen 361005,China 2Shanghai Institute of Materia Medica,Shanghai Institutes of Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,China 3key laboratory for Bioorganic Phosphorus Chemistry and chemical biology,Ministry of Education,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua University,Beijing 100084,China 1. key laboratory for chemical biology of fujian province Department of Chemical Biology Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China2. Shanghai Institute of Materia Medica Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 201203 China3. Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology Ministry of Education Department of Chemistry School of Life Sciences and Engineering Tsinghua University Beijing 100084 China

Seryl-Histidine dipeptide(Ser-His) has been previously reported to be capable of cleaving DNAs and carboxyl esters,as well as *** protein cleavage mechanism has not been addressed *** an initial step of protein cleavage activity,the non-covalent binding affinity of Ser-His for proteins is a crucial *** this work,we took cyclophilin A(CyPA) as a substrate protein,and evaluated the non-covalent interaction between CyPA and Ser-His using a combination of NMR spectroscopy and molecular modeling *** independent Ser-His binding sites on CyPA were detected using 15N-1H heteronuclear single-quantum coherence(HSQC) *** binding site binds one Ser-His *** constants,Kd1 and Kd2,were estimated to be 2.07 and 6.66 mmol/L,respectively,indicative of the weak non-covalent interaction between Ser-His and *** on molecular modeling results,we suggest that both the α-amino and the side chain hydroxyl group of Ser-His are crucial for the non-covalent interaction between Ser-His and *** work sheds light on the molecular mechanism of Ser-His and its analogues cleaving proteins.

关键词： Ser-His cyclophilin A HSQC non-covalent interaction binding sites

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High CHIP Expression Is Associated With The Generation Of Esophageal Carcinoma

High CHIP Expression Is Associated With The Generation Of Es...

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第六届全国化学生物学学术会议

作者： Fangfang Zheng,ChengYan Wu,Yufen Zhao~*,Xueji Wu~* Department of chemical biology and The key laboratory for chemical biology of fujian province, College of Chemistry and chemical Engineering,Xiamen University,Xiamen 361005,China.

＜正＞Background and Objectives:C-terminus of Hsp70 interacting protein（CHIP） ubiquitinates the abnormal proteins which could not be refolded exactly by *** ubiquitinated protein should be degraded by the *** abnormal proteins should be expressed in cancer cell because of disordered regulation in the *** carcinoma is one of the most lethal tumors,and many people all over the

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Overexpression of Pin1 in esophageal cancer

Overexpression of Pin1 in esophageal cancer

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第六届全国化学生物学学术会议

作者： Hongbao Xie,ChengYan Wu,Yufen Zhao~*,Xueji Wu~* Department of chemical biology and The key laboratory for chemical biology of fujian province, College of Chemistry and chemical Engineering,Xiamen University,Xiamen 361005,China.

＜正＞Peptidyl-prolyl isomerase Pinl specifically catalyzes cis/trans-isomerization of proline in the target sequence of phosphorylated Ser/Thr-Pro in over 50 critical regulatory *** is abnormally over-expressed in a ...

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Applications of CD to Structural Characterization of Chiral Compounds

Applications of CD to Structural Characterization of Chiral ...

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中国化学会第五届全国结构化学学术会议

作者： Hui Zhang Leiqi Chen Lei Ding Fang Wang Yufen Zhao Department of Chemistry and key laboratory for chemical biology of fujian province,College of Chemistry and chemical Engineering,Xiamen University,361005,fujian

＜正＞Circular dichroism（CD）spectroscopy as an especial technique of fingerprint has all along played an important role in structural characterization of chiral compounds,which can provide useful information on the absolute configuration and conformation of chiral molecules,enantiomeric excess of the chiral substances and something about reaction *** a major tool in chiral solid-state chemistry,solid-state CD is currently attracting a great deal attention since it should offer invaluable spectral information unobtainable from solution CD;for example,of particular interest is an unusual conformation of chiral molecules that is unstable in *** solution and solid-state CD with UV-Vis spectra and other structural analysis including X-ray crystallographic data may gain a comprehensive insight into the details of the structural features, electronic levels and electronic transitions of chiral compounds.

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The discovery of the mini-protease:Seryl Histidine Dipeptide

The discovery of the mini-protease:Seryl Histidine Dipeptide

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中国化学会第26届学术年会化学生物分会场

作者： Xiao-xia Liu, Yan Liu, Ming-Kun Lin, Yu-fen Zhao* The key laboratory for chemical biology of fujian province, Department of chemical biology, College of Chemistry and chemical Engineering, Xiamen University, Xiamen 361005

Our previous researches showed that Seryl-Histidine dipeptide (Ser-His) could cleave proteins such as bovine serum albumen (BSA) [1] and green fluorescence protein (GFP) [4] over wide ranges of pH and temperature, as well as DNA [1], with a hydrolysis mechanism. Therefore, Ser-His is the shortest peptide with the hydrolysis cleavage activity on protein, and its function is similar to serine protease. Recently, the discovery of extended substrate specificity of opossum chymase illuminated the origin of mast cell chymases [5, 6]. So, to investigate the function of Ser-His further might provide more information to understand the function group evolution for the origin of serine protease. In this paper, high resolution mass spectrometry was applied to study the cleavage sites of Seryl-Histidine dipeptide (Ser-His) on two different proteins, namely green fluorescence proteins (xm18) and cyclophilin A. It was found that the two different protein, were both cleaved by Ser-His into small polypeptide fragments. Through comparison of the cleavage activity of Ser-His and Protease K, it means that the hydrolysis activity of Ser-His is about one thirty thousandths of that of Protease K. Although Ser-His possesses non-specificity cleavage activity on the primary sequence of substrate proteins, but, amino acid residue, F, is the more favorite cleavage site (Fig. 1 and Fig. 2), which is similar to the natural serine protease, chymotrypsin. Therefore, the smallest peptide, Ser-His dipeptdie, was named the as mini-protease. It implied that Ser-His is probably the origin of serine proteases.

关键词： Ser-His diepetide mini-protease cleavage selectivity evolutionary origin

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ATP-ADP exchange activity and molecular chaperone activity of Hsp70

ATP-ADP exchange activity and molecular chaperone activity o...

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第六届全国化学生物学学术会议

作者： Huawei Jin,ChengYan Wu,Fangfang Zheng,Yufen Zhao~*,Xueji Wu~* Department of chemical biology and The key laboratory for chemical biology of fujian province, College of Chemistry and chemical Engineering,Xiamen University,Xiamen 361005,China.

Hsp70 is a multifunctional molecular chaperone whose interactions with protein substrates are regulated by ATP hydrolysis and ATP-ADP ***70 exists weakly ATPase activity and locates at the N-teminal 44-kD ***,the wide-type and mutant(K71A,T204A and H227S) human Hsp70 were expressed in *** and purified on HisTrap Ni-agarose column and then

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Copper-dependent strand scission in DNA induced by Shikonin

Copper-dependent strand scission in DNA induced by Shikonin

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第六届全国化学生物学学术会议

作者： HongMingCheng,Zhen Wu~*,YuFen Zhao Department of Chemistry,Department of Pharmaceutical Science,and key laboratory for chemical biology of fujian province,Xiamen University,Xiamen 361005,China.

＜正＞Shikonin（β-alkannin）,a naphthazarin derivative extracted from Lithospermum erythrorhizon *** Zucc,has shown a variety of abilities such as accelerating tissue granulation proliferation and exerting antibacterial,anti-inflammatory,and anti-tumor *** the presence of Cu（Ⅱ）,shikonin caused breakage of calf thymus and supercoiled plasmid pBR322 *** metal ions tested（Mg（Ⅱ）,Ca（Ⅱ）,and Ni（Ⅱ）） were ineffective and only Fe（Ⅱ） has the same ability in the DNA breakage *** involvement of active oxygen in the reaction was established by the inhibition of DNA breakage by superoxide dismutase,catalase,thiourea,sodium azide,potassium iodide and sodium ***（Ⅰ） was shown to be an essential intermediate by using the Cu（Ⅰ）-specific sequestering reagent *** induced HeLa cell apoptosis involved in the mechanism of increasing in intracellular reactive oxygen *** was suggested that shikonin generated reactive oxygen species as a prooxidant in the presence of transition metals in cells which resulting in DNA damage.

关键词： DNA cleavage Cu(Ⅱ) reduction shikonin

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PEPTIDE BOND FORMATION ON C-TERMINAL OF PEPTIDES IN ORGANIC SOLUTION BY INDUCEMENT OF N-PHOSPHORYL AMINO ACID

PEPTIDE BOND FORMATION ON C-TERMINAL OF PEPTIDES IN ORGANIC ...

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第17届国际磷化学大会

作者： Liu Xiaoxia Liu Yan Zhao Yufen* The key laboratory for chemical biology of fujian province Department of Chemistry and College of Chemistry and chemical Engineering Xiamen University Xiamen 361005 P. R. China

＜正＞Peptide formation is one of the most important reactions in prebiotic evolution. And N-phosphoryl-α-amino acids were proposed as model for the co-evolution of proteins and nucleic

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STUDIES ON THE NONCOVALENT BONDING BETWEEN ATP AND α-AMINOPHOSPHONIC ACIDS DERIVATIVES BY ESI-MS AND MOLECULAR MODELING

STUDIES ON THE NONCOVALENT BONDING BETWEEN ATP AND α-AMINOP...

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第17届国际磷化学大会

作者： Liu Xiaoxia Fang Hua Liu Yan Zhao Yufen* The key laboratory for chemical biology of fujian province Department of Chemistry and College of Chemistry and chemical Engineering Xiamen University Xiamen 361005 P. R. China

＜正＞To investigate molecular recognition of ATP with proteins or structured peptide-based receptor is very important for understanding enzymatic mechanisms and drug design [1,2].

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