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DNA-directed assembly of copper nanoblocks with inbuilt fluorescent and electrochemical properties： Application in simultaneous amplification-free analysis of multiple RNA species

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Nano Research 2018年第2期11卷 940-952页

作者： kevin m. Koo Laura G. Carrascosa matt Trau Centre for Personalized Nanomedicine Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland Brisbane Australia School of Chemistry and molecular Biosciences The University of Queensland Brisbane Australia

The intrinsic affinity of DNA molecules toward metallic ions can drive the specific formation of copper nanostructures within the nucleic acid helix structure in a sequence-dependent manner. The resultant nanostructures have interesting fluorescent and electrochemical properties, which are attractive for novel biosensing applications. However, the potential of using DNA-templated nano- structures for precision disease diagnosis remains unexplored. Particularly, DNA- templated nanostructures show high potential for the universal amplification-free detection of different RNA biomarker species. Because of their low cellular levels and differing species-dependent length and sequence features, simultaneous detection of different messenger RNAs, microRNAs, and long non-coding RNAs species with a single technique is challenging. Here, we report a contemporary technique for facile in situ assembly of DNA-templated copper nanoblocks （CuNBs） on various RNA species targets after hybridization-based magnetic isolation. Our approach circumvents the typical limitations associated with amplification and labeling procedures of current RNA assays. The synthesized CuNBs enabled amplification-free fm-level RNA detection with flexible fluorescence or electrochemical readouts. Furthermore, our nanosensing technique displays potential for clinical application, as demonstrated by non-invasive analysis of three diagnostic RNA biomarkers from a cohort of 10 prostate cancer patient urinary samples with 100%-concordance （quantitative reverse transcription- polymerase chain reaction （PCR） validation）. The good analytical performance and versatility of our method may be useful in both diagnostics and research fields.

关键词： prostate cancer RNA detection DNA-templated coppernanostTuctures amplification-free fluorescence detection electrochemical detection

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Carbon star CGCS 673 identified as a semi-regular variable star

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Research in Astronomy and Astrophysics 2020年第11期20卷 235-238页

作者： Stephen m.Brincat Charles Galdies kevin Hills Flarestar Observatory(mPC 171) Fl.5/BGeorge Tayar StreetSan GwannSGN 3160Malta Institute of Earth Systems University of MaltaMsidaMSD 2080Malta Tacande Observatory(La Palma Spain)c/o 4 Grange DriveHartfordCheshire CW83AEUK

This study reports that the carbon star CGCS 673 is a semi-regular(SR)variable star with a period of 135 d and an amplitude of 0.18mag in the *** light curve obtained by this study correlates well with the SR classification as the photometric data obtained show noticeable periodicity in the light changes of CGCS 673 that is occasionally interrupted by a period of irregular *** derived period and colour index obtained from our data and those from professional databases indicate that the attributes of this star fall within the parameters of the SR class of variable *** our notification of the discovery that this star is a variable source,CGCS 673 has received the AAVSO Unique Identifier of(AAVSO UID)000-BmZ-492.

关键词： techniques photometric stars variables semi-regular variable stars individual CGCS

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Author Correction:Enhanced CD19 activity in B cells contributes to immunodeficiency in mice deficient in the ICF syndrome gene Zbtb24

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Cellular & molecular Immunology 2024年第1期21卷 100-100页

作者： Zhengzhou Ying Swanand Hardikar Joshua B.Plummer Tewfik Hamidi Bin Liu Yueping Chen Jianjun Shen Yunxiang mu kevin m.mcBride Taiping Chen Department of Epigenetics and molecular Carcinogenesis The University of Texas MD Anderson Cancer Center Houston TX 77030 USA Program in Genetics and Epigenetics The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences Houston TX 77030 USA

In the sentence beginning‘To trace cell proliferation,FO and mZ B cells’and the sentence beginning‘For cell activation,naïve B cells or CH12F3 cells’in this article,the[10 ng/ml]‘F(ab’)_(2)fragment goat anti-mouse Igm’should have read‘10μg/ml F(ab’)_(2)fragment goat anti-mouse Igm’.The original article has been corrected.

关键词： immunodeficiency CD19 deficient

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WHAT DOES ACCESS TO JUSTICE REQUIRE？ -- OVERCOmING BARRIERS TO INVOKE THE UNITED NATIONS CONVENTION ON THE RIGHTS OF PERSONS WITH DISABILITIES

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Frontiers of Law in China-Selected Publications from Chinese Universities 2016年第2期11卷 280-322页

作者： kevin m. Cremin Columbia Law School mFY Legal Services Inc. New York US

Persons with disabilities have a right to effective access to justice under the United Nations Convention on the Rights of Persons with Disabilities （UNCRPD）. This article provides insights on the parameters of that right, including a close examination of the history and text of Article 13, which directly addresses access to justice and other relevant UNCRPD provisions. In addition to the UNCRPD, this article discusses implementation guidance from the Committee on the Rights of Persons with Disabilities, including its guidelines for State Party reports and jurisprudence. The initial reports by eleven States Parties -- Argentina, Azerbaijan, China, Costa Rica, Croatia, Dominican Republic, Ecuador, Hungary, mexico, Peru and Turkmenistan - are also considered. The Committee＇s feedback regarding implementation of Article 13 by these eleven States parties is critiqued for being limited and inconsistent. This article then attempts to clarify what effective access to justice actually requires. It does so by focusing on the insights that can be drawn from implementation of Article 13 since the UNCRPD was adopted as well as implementation guidance from the Conference of States Parties, the International Disability Alliance, the World Network of Users and Survivors of Psychiatry and the National Center for Access to Justice. This article concludes with recommendations on how the Committee can improve its guidance on access to justice to help ensure that equal rights will not be illusory for persons with disabilities.

关键词： access to justice Convention on the Rights of Persons with Disabilities right tocounsel Committee on the Rights of Persons with Disabilities

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Elements of disturbance that affect epiphyte vitality in a temperate rainforest:an experimental approach

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Journal of Plant Ecology 2019年第2期12卷 306-313页

作者： Nalini m.Nadkarni kevin D.Kohl Department of Biology University of Utah257 South 1400 EastSalt Lake CityUT 84112USA Department of Biological Sciences University of Pittsburgh403B Clapp Hall4249 Fifth AvenuePittsburghPA 115260USA

Aims Epiphytes are an abundant and diverse component of many wet temperate forests and have significant roles in ecosystem *** is known about the processes and rates of their death and decomposition when they fall from the canopy,which limits our understanding of their role in forest carbon sequestration and nutri-ent *** the temperate rainforest of the Quinault River Valley,Washington State,our aim was to test hypotheses regarding four elements of disturbance that might contribute to their *** We established set of experiments in which we placed samples of canopy epiphytes and their branch segments:(i)in the canopy versus forest floor microenvironment(stratum);(ii)attached to live versus dead branch substrates;(iii)subjected to physical disruption and‘jarring’;and(iv)in direct versus indirect con-tact with the forest *** the 2-year study,we assigned a non-destructive‘vitality index’(based on color and appar-ent mortality and dryness)to each sample every 2-3 months to compare effects of the experimental treatments and analyzed with a statistical model and post hoc pairwise comparisons of *** Findings The canopy versus ground stratum and live/dead branch status sig-nificantly affected epiphyte *** of physical disruption and ground contact were not *** were seasonal effects(low vitality during the sampling times in the summer,revitalization upon sampling times in the winter)for all treatments except samples in contact with the *** implication of these results relates to effects of climate change,which is predicted to shift to hotter,drier summers and wetter *** change may affect forest dynamics and nutrient cycling in unpredictable *** also point to future experiments to understand biotic and abiotic effects on epiphyte disturbance and dynamics.

关键词： disturbance epiphytes forest canopy forest dynamics moss Olympic rainforest

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Sonicating bees demonstrate flexible pollen extraction without instrumental learning

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Current Zoology 2019年第4期65卷 425-436页

作者： Callin m. Switzer Avery L. Russell Daniel R. Papaj Stacey A. Combes Robin hopkins Department of Organismic and Evolutionary Biology Harvard University Cambridge MA USA Arnold Arboretum Harvard University Boston MA USA Department of Ecology and Evolutionary Biology University of Arizona Tucson AZ USA Department of Neurobiology Physiology and Behavior University of California at Davis Davis CA USA Department of Biology and eScience Institute University of Washington Seattle WA USA Department of Biological Sciences University of Pittsburgh Pittsburgh PA USA

Pollen collection is necessary for bee survival and important for flowering plant reproduction, yet if and how pollen extraction motor routines are modified with experience is largely unknown. Here, we used an automated reward and monitoring system to evaluate modification in a common pollen-extraction routine, floral sonication. Through a series of laboratory experiments with the bumblebee, Bombus impatiens, we examined whether variation in sonication frequency and acceleration is due to instrumental learning based on rewards, a fixed behavioral response to rewards, and/or a mechanical constraint. We first investigated whether bees could learn to adjust their sonication frequency in response to pollen rewards given only for specified frequency ranges and found no evidenee of instrumental learning. However, we found that absenee versus receipt of a pollen reward did lead to a predictable behavioral resp on se, which depe nded on bee size. Fin ally, we found some evide nee of mechanical con straints, in that flower mass affected sonication acceleration (but not frequency) through an interaction with bee size. In generalz larger bees showed more flexibility in sonication frequency and acceleration, potentially reflecting a size-based constraint on the range over which smaller bees can modify frequency and accelerati on. Overall our results show that although bees did not display instrumental learning of sonication frequency, their sonication motor routine is nevertheless flexible.

关键词： Bombus impatiens buzz pollination foraging innate behavior learned behavior Solanum

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Capacitance,charge dynamics,and electrolyte-surface interactions in functionalized carbide-derived carbon electrodes

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Progress in Natural Science:materials International 2015年第6期25卷 631-641页

作者： Boris Dyatkin Eugene mamontov kevin m.Cook Yury Gogotsi Department of materials Science & Engineering and A.J.Drexel Nanomaterials Institute Drexel University Spallation Neutron Source Oak Ridge National Laboratory materials Engineering Division Naval Air Systems Command

This study analyzed the dynamics of ionic liquid electrolyte inside of defunctionalized,hydrogenated,and aminated pores of carbide-derived carbon supercapacitor *** approach tailors surface functionalities and tunes nanoporous structures to decouple the influence of pore wall composition on capacitance,ionic resistance,and long-term ***-elastic neutron scattering probes the self-diffusion properties and electrode-ion interactions of electrolyte molecules confined in functionalized ***-temperature ionic liquid interactions in confined pores are strongest when the hydrogen-containing groups are present on the *** property translates into higher capacitance and greater ion transport through pores during electrochemical *** hydrogenated pores,aminated pores do not favorably interact with ionic liquid ions and,subsequently,are outperformed by defunctionalized surfaces.

关键词： Supercapacitor Carbide-derived carbon Neutron scattering Energy storage Porous materials Surface chemistry

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Rapid flood inundation mapping by differencing water indices from pre-and post-flood Landsat images

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Frontiers of Earth Science 2021年第1期15卷 1-11页

作者： Ramesh SIVANPILLAI kevin m.JACOBS Chloe m.mATTILIO Ela V.PISKORSKI Wyoming Geographic Information Science Center University of WyomingLaramieWY 82071USA Department of Ecosystem Science&management University of WyomingLaramieWY 82071USA Haub School of Environment&Natural Resources University of WyomingLaramieWY 82071USA Department of Plant Sciences University of WyomingLaramieWY 82071USA

Following flooding disasters,satellite images provide valuable information required for generating flood inundation *** or optical imagery can be used for generating flood maps when the inundated areas are not covered by *** propose a rapid mapping method for identifying inundated areas based on the increase in the water index value between the pre-and post-flood satellite *** of the Normalized Difference Water Index(NDWI)and modified NDWI(mNDWI)will be higher in the post-flood image for flooded areas compared to the pre-flood *** on a threshold value,pixels corresponding to the flooded areas can be separated from non-flooded *** maps derived from differencing mNDWI values accurately captured the flooded *** the output image will be influenced by the choice of the pre-flood image,hence analysts have to avoid selecting pre-flood images acquired in drought or earlier flood *** the inundation maps generated using this method have to be overlaid on the post-flood satellite image in order to orient personnel to landscape *** of the proposed technique are that flood impacted areas can be identified rapidly,and that the pre-existing water bodies can be excluded from the inundation *** pairs of other satellite data,several maps can be generated within a single flood which would enable emergency response agencies to focus on newly flooded areas.

关键词： Rapid Flood mapping(RFm) inundation maps Satellite data NDWI mNDWI

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Prognostic model of survival outcomes in non-small cell lung cancer patients initiated on afatinib: pooled analysis of clinical trial data

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Cancer Biology & medicine 2019年第2期16卷 341-349页

作者： Ashley m. hopkins Adel Shahnam Sasha Zhang Chris S. Karapetis Andrew Rowland michael J. Sorich Flinders Centre for Innovation in Cancer Department of Clinical Pharmacology College of Medicine and Public Health Flinders University Adelaide 5042 Australia The Canberra Hospital Garran 2605 Australia

Objective: Several predictors of survival have been identified in EGFR-positive non-small cell lung cancer(NSCLC) patients treated with first generation EGFR inhibitors. Prognostic models of survival outcomes with afatinib have not been ***: A prognostic tool for overall survival(OS)/progression free survival(PFS) based on pre-treatment clinicopathological factors was developed for EGFR-positive advanced NSCLC patients treated with first-line afatinib using penalised regression of individual-participant data from LUX-Lung 3 and 6(n = 468). Favourable, intermediate and poor risk groups were identified and externally validated using LUX-Lung 1(n = 390) and LUX-Lung 2(n = 129) trials that initiated afatinib following previous chemotherapy or EGFR inhibitor ***: Discriminative performance was good in the development and validation cohorts. For patients treated with first-line afatinib, the median OS for the favourable, intermediate and poor risk groups were > 47.7, 29.3 and 16.4 months, respectively, and the median PFS were 17.3, 13.2 and 8.3 months, respectively. The improvement in median OS with afatinib use compared to chemotherapy was > 12.4 months for the favourable risk group, whereas no OS benefit was apparent for the poor risk group. The improvement in median PFS with afatinib use compared to chemotherapy was 10.2 months for the favourable risk group and 3.2 months for the poor risk ***: A prognostic tool was developed and validated to identify favourable, intermediate and poor risk groups for OS/PFS in EGFR-positive advanced NSCLC patients treated with afatinib. The prognostic groups can inform the likely absolute OS/PFS benefit expected from afatinib compared to chemotherapy in first-line treatment.

关键词： Afatinib non-small cell lung cancer prognostic model

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ezQTL:A Web Platform for Interactive Visualization and Colocalization of QTLs and GWAS Loci

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Genomics, Proteomics & Bioinformatics 2022年第3期20卷 541-548页

作者： Tongwu Zhang Alyssa Klein Jian Sang Jiyeon Choi kevin m.Brown Division of Cancer Epidemiology and Genetics National Cancer InstituteBethesdaMD 20892USA

Genome-wide association studies(GWAS)have identified thousands of genomic loci associated with complex diseases and traits,including *** vast majority of common traitassociated variants identified via GWAS fall in non-coding regions of the genome,posing a challenge in elucidating the causal variants,genes,and mechanisms *** quantitative trait locus(eQTL)and other molecular QTL studies have been valuable resources in identifying candidate causal genes from GWAS loci through statistical colocalization *** QTL colocalization is becoming a standard analysis in post-GWAS investigation,an easy web tool for users to perform formal colocalization analyses with either user-provided or public GWAS and eQTL datasets has been ***,we present ezQTL,a web-based bioinformatic application to interactively visualize and analyze genetic association data such as GWAS loci and molecular QTLs under different linkage disequilibrium(LD)patterns(1000 Genomes Project,UK Biobank,or user-provided data).This application allows users to perform data quality control for variants matched between different datasets,LD visualization,and two-trait colocalization analyses using two state-of-the-art methodologies(eCAVIAR and HyPrColoc),including batch *** is a free and publicly available cross-platform web tool,which can be accessed online at https://***/ezqtl.

关键词： Genome-wide association study Expression quantitative trait locus Colocalization Linkage disequilibrium Visualization

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