DNA-directed assembly of copper nanoblocks with inbuilt fluorescent and electrochemical properties： Application in simultaneous amplification-free analysis of multiple RNA species

作     者：Kevin M. Koo Laura G. Carrascosa Matt Trau 

作者机构：Centre for Personalized Nanomedicine Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland Brisbane Australia School of Chemistry and Molecular Biosciences The University of Queensland Brisbane Australia 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2018年第11卷第2期

页      面：940-952页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 0808[工学-电气工程] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the UQ Postdoctoral Research Fellowship funding received by our laboratory from the National Breast Cancer Foundation of Australia support from the Australian Government Research Training Program Scholarship 

主　　题：prostate cancer,RNA detection,DNA templated coppernanostTuctures,amplification free,fluorescence detection,electrochemical detection 

摘      要：The intrinsic affinity of DNA molecules toward metallic ions can drive the specific formation of copper nanostructures within the nucleic acid helix structure in a sequence-dependent manner. The resultant nanostructures have interesting fluorescent and electrochemical properties, which are attractive for novel biosensing applications. However, the potential of using DNA-templated nano- structures for precision disease diagnosis remains unexplored. Particularly, DNA- templated nanostructures show high potential for the universal amplification-free detection of different RNA biomarker species. Because of their low cellular levels and differing species-dependent length and sequence features, simultaneous detection of different messenger RNAs, microRNAs, and long non-coding RNAs species with a single technique is challenging. Here, we report a contemporary technique for facile in situ assembly of DNA-templated copper nanoblocks （CuNBs） on various RNA species targets after hybridization-based magnetic isolation. Our approach circumvents the typical limitations associated with amplification and labeling procedures of current RNA assays. The synthesized CuNBs enabled amplification-free fM-level RNA detection with flexible fluorescence or electrochemical readouts. Furthermore, our nanosensing technique displays potential for clinical application, as demonstrated by non-invasive analysis of three diagnostic RNA biomarkers from a cohort of 10 prostate cancer patient urinary samples with 100%-concordance （quantitative reverse transcription- polymerase chain reaction （PCR） validation）. The good analytical performance and versatility of our method may be useful in both diagnostics and research fields.