检索结果-南通市图书馆

Neural Regeneration Research 2012年第12期7卷 885-890页

作者： Zhanwei Zhang Ting Zhang keli dong Department of Neurosurgery First Affiliated Hospital Hunan University of Chinese Medicine Changsha 410007 Hunan Province China Department of Traditional Chinese Medicine Third Xiangya Hospital Central South University Changsha 410013 Hunan Province China

At 8 weeks after intragastric administration of icariin to senescence-accelerated mice （P8 strain）, Morris water maze results showed that escape latency was shortened, and the number of platform crossings was increased. Immunohistochemical staining and western blot assay detected significantly increased levels of cyclic adenosine monophosphate response element binding protein These results suggest that icariin upregulates phosphorylated cyclic adenosine monophosphate response element binding protein levels and improves learning and memory functions in hippocampus of the senescence-accelerated mouse.

关键词： icariin Alzheimer's disease hippocampus phosphorylated cyclic adenosine monophosphate response element binding protein neural regeneration

Yizhijiannao Granule and a combination of its effective monomers,icariin and Panax notoginseng saponins,inhibit early PC12 cell apoptosis induced by beta-amyloid(25-35)

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2012年第24期7卷 1845-1850页

作者： Ting Zhang Zhanwei Zhang keli dong Guangcheng Li Hong Zhu Department of Traditional Chinese Medicine Xiangya Third HospitalCentral South UniversityChangsha 410013Hunan ProvinceChina Department of Neurosurgery First Affiliated Hospital of Hunan University of Chinese MedicineChangsha 410007Hunan ProvinceChina

One of our previous studies showed that Yizhijiannao Granule,a compound Chinese medicine, effectively improved the clinical symptoms of Alzheimer’s *** the present study,we established a model of Alzheimer’s disease using beta-amyloid（25-35）in PC12 cells,and treated the cells with Yizhijiannao Granule and its four monomers,i.e.,icariin,catechin,Panax notoginseng saponins,and eleutheroside *** cytometry showed that Yizhijiannao Granule-containing serum, icariin,Panax notoginseng saponins,and icariin＋Panax notoginseng saponins were protective against beta-amyloid（25-35）-induced injury in PC12 *** in combination with Panax notoginseng saponins significantly inhibited early apoptosis of PC12 cells with beta-amyloid （25-35）-induced injury compared to icariin or Panax notoginseng saponins *** effects of icariin＋Panax notoginseng saponins were similar to the effects of Yizhijiannao *** findings indicate that two of the effective monomers of Yizhijiannao Granule,icariin and Panax notoginseng saponins,can synergistically inhibit early apoptosis of PC12 cells induced by beta-amyloid（25-35）.

关键词： Alzheimer’s disease icariin Panax notoginseng Saponins Yizhijiannao Granule Chinese medicine monomer beta-amyloid protein PC12 cell Chinese medicine neural regeneration

Icariin and differential protein expression in the entorhinal area of senescence-accelerated mouse prone 8 mice

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2011年第18期6卷 1370-1374页

作者： Ting Zhang Zhanwei Zhang keli dong Guangcheng Li Hong Zhu Department of Chinese Medicine Third Xiangya Hospital of Central South University Changsha 410013 Hunan Province China Department of Neurosurgery First Hospital of Hunan University of Chinese Medicine Changsha 410007 Hunan Province China

The present study sought to explore the mechanism of action by which icariin, an active component of Epimedii Herba, treats Alzheimer＇s disease at the proteomics level. Two-dimensional gel electrophoresis was used to isolate total protein from the entorhinal cortex of senescence-accelerated mouse prone 8 （SAMP8） mice, and differential protein spots were obtained. Corresponding peptide mass fingerprinting was conducted through mass spectrography to identify differential protein spots. Twenty-six differential protein spots were found in the entorhinal area of SAMP8 mice at 8 weeks following intragastdc perfusion with icariin and double distilled water. Fourteen spots were identified, which were involved in mitochondrial energy metabolism, oxidative stress, and neuronal function. The results revealed that icafiin can regulate the expression of various proteins in the entorhinal cortex of SAMP8 mice, and treat Alzheimer＇s disease by improving mitochondfial function, suppressing oxidative stress, inhibiting neural cell apoptosis, and protecting neurons.

关键词： icariin Alzheimer's disease entorhinal cortex proteomics traditional Chinese medicine neural regeneration

Treating Alzheimer's disease with Yizhijiannao granules by regulating expression of multiple proteins in temporal lobe

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2014年第13期9卷 1283-1287页

作者： Hong Zhu Liuyang Luo Sihang Hu keli dong Guangcheng Li Ting Zhang Department of Traditional Chinese Medicine Third Xiangya Hospital of Central South University Department of Emergency Bao-an District Hospital of Traditional Chinese Medicine

Yizhijiannao granules have been shown to improve cognitive function in Alzheimer＇s disease patients. The present study sought to explore the mechanisms involved in the cognitive enhancing effects of Yizhijiannao granule. Senescence-accelerated mouse prone 8 mice with learning and memory disorders were intragastrically treated with Yizhijiannao granule for 8 weeks. Mice intragastrically treated with double distilled water for 8 weeks were considered as the control group. 2D gel electrophoresis was used to isolate total protein from the temporal lobe of senescence-accelerated mouse prone 8 mice, and differential protein spots were obtained by mass spectrometry. Thirty-seven differential protein spots were found in the temporal lobe area of both groups. Ten protein spots were identified： high mobility group box 1, dimethylarginine dimethylaminohydrolase-1, nenroglobin, hemoglobin beta adult major chain, peroxiredoxin-6, cofilin-1, flotiUin 1, peptidylprolyl isomerase A, voltage-dependent anion channel-2 and chaperonin containing TCP1, and subunit 2. Among other functions, these proteins are separately involved in the regulation of amyloid beta production, oxidative stress, neuroinflammation, regulation of tau phosphorylation, and regulation of neuronal apoptosis. Our results revealed that Yizhijiannao granule can regulate the expression of various proteins in the temporal lobe of senescence-accelerated mouse prone 8 mice, and may be therapeutically beneficial for the treatment of Alzheimer＇s disease.

关键词： nerve regeneration traditional Chinese medicine neurodegeneration Alzheimer'sdisease Yizhijiannao granule mass spectrometry cognition neural regeneration

在线全文

学校读者我要写书评

暂无评论

试谈高校图书馆人员的组配与素质培养

天津体育学院学报 1996年第3期11卷 81-83页

作者：董克礼天津体育学院

藏书建设工作是图书馆工作的核心内容，高校图书馆必须遵循“革命化、年轻化、知识化、专业化”的原则组配队伍，才能培养出具有实际工作能力，宽阔知识领域和高尚职业道德的图书馆工作人员。

关键词：高校图书馆人员组配素质培养

在线全文

学校读者我要写书评

暂无评论

从天津体院图书馆看高校图书馆改革与发展

天津体育学院学报 1997年第1期12卷 85-88页

作者：董克礼天津体育学院图书馆

在回顾天津体院图书馆十几年来改革实践的基础上。

关键词：高校图书馆改革发展

2型糖尿病血瘀证患者脂联素及肿瘤坏死因子α水平的研究

在线全文

学校读者我要写书评

暂无评论

新中医 2007年第10期39卷 81-82页

作者：高自芳董克礼浏阳市人民医院内一科湖南浏阳410300 中南大学湘雅三医院中医科湖南长沙410013

目的:观察2型糖尿病血瘀证患者血清脂联素及肿瘤坏死因子α(TNF-α)水平的变化。方法:对30例血瘀证与30例非血瘀证的2型糖尿病患者(糖尿病组)血清脂联素、TNF-α及胰岛素抵抗指数等指标进行检测和比较,并以30例健康者作为正常对照组。结... 详细信息

目的:观察2型糖尿病血瘀证患者血清脂联素及肿瘤坏死因子α(TNF-α)水平的变化。方法:对30例血瘀证与30例非血瘀证的2型糖尿病患者(糖尿病组)血清脂联素、TNF-α及胰岛素抵抗指数等指标进行检测和比较,并以30例健康者作为正常对照组。结果:空腹血糖(FBG)糖尿病组与正常对照组比较,差异有非常显著性意义(P<0.01)。TNF-α、脂联素、胰岛素抵抗指数(IRI),糖尿病组与正常对照组比较,差异有显著性或非常显著性意义(P<0.05,P<0.01),而糖尿病组血瘀证与非血瘀证各项指标比较,差异有非常显著性意义(P<0.01)。结论:糖尿病胰岛素抵抗可能参与了血瘀证的形成,TNF-α、脂联素可作为糖尿病血瘀证的重要标志物之一。

关键词： 2型糖尿病血瘀证肿瘤坏死因子-α 脂联素

复方菝葜颗粒对非小细胞肺癌A549细胞P53基因、细胞周期的影响

在线全文

学校读者我要写书评

暂无评论

中国现代医学杂志 2009年第7期19卷 1025-1027页

作者：胡灿红李广诚董克礼江苏省中西结合医院肿瘤科江苏南京210028 中南大学湘雅三医院中医科湖南长沙410013

目的观察复方菝葜颗粒对非小细胞肺癌A549细胞P53基因及细胞周期的影响,探讨复方菝葜颗粒治疗非小细胞肺癌的部分作用机制。方法雄性SD大鼠30只,随机分为两组,每组15只。一组灌服复方菝葜颗粒,2次/d;另一组灌服等剂量蒸馏水,第6次灌胃... 详细信息

目的观察复方菝葜颗粒对非小细胞肺癌A549细胞P53基因及细胞周期的影响,探讨复方菝葜颗粒治疗非小细胞肺癌的部分作用机制。方法雄性SD大鼠30只,随机分为两组,每组15只。一组灌服复方菝葜颗粒,2次/d;另一组灌服等剂量蒸馏水,第6次灌胃后2h提取血清。A549细胞株随机分为中药实验组、西药对照组、正常对照组,分别加入10%的含药血清、顺铂3μg/mL和10%SD大鼠血清,培养48h后收集细胞,RT-PCR测定P53mRNA表达;流式细胞技术检测细胞周期。结果中药实验组和西药对照组G1期细胞数及P53mRNA相对表达量高于正常对照组相(P<0.05)。结论A549细胞存在P53mRNA低表达,复方菝葜颗粒能够上调抑癌基因P53mRNA表达,并将癌变的细胞阻滞在G1期,这可能是复方菝葜颗粒治疗非小细胞肺癌的作用机制之一。

关键词：复方菝葜颗粒非小细胞肺癌细胞周期

益智健脑颗粒对阿尔茨海默病患者外周血清中IL-1β与TNF-α表达的影响

在线全文

学校读者我要写书评

暂无评论

湖南中医杂志 2010年第5期26卷 1-3页

作者：刘佩董克礼中南大学湘雅三医院 410013

目的:探讨益智健脑颗粒对阿尔茨海默病(AD)患者外周血清中IL-1β与TNF-α表达的影响。方法:严格按照配对的原则选择AD患者及健康老人,应用双抗体夹心酶联免疫吸附测定(ELISE)检测IL-1β与TNF-α表达的水平。结果:血清IL-1β含量AD组与... 详细信息

目的:探讨益智健脑颗粒对阿尔茨海默病(AD)患者外周血清中IL-1β与TNF-α表达的影响。方法:严格按照配对的原则选择AD患者及健康老人,应用双抗体夹心酶联免疫吸附测定(ELISE)检测IL-1β与TNF-α表达的水平。结果:血清IL-1β含量AD组与正常对照组比较,差异有显著性(P0.05);中药组治疗前后IL-1β比较,P0.05)。结论:(1)AD患者血清IL-1β、TNF-α水平高于正常老年人,这可能与AD脑内炎症反应有关;(2)益智健脑颗粒可通过抑制细胞炎性因子而对AD患者发挥其治疗作用。

关键词：阿尔茨海默病中医药疗法益智健脑颗粒

在线全文

学校读者我要写书评

暂无评论

益智健脑颗粒治疗阿尔茨海默氏病20例总结

湖南中医杂志 2006年第2期22卷 3-4页

作者：董克礼曾望远中南大学湘雅三医院 410013

目的:观察益智健脑颗粒治疗阿尔茨海默氏病的临床疗效。方法:治疗组20例采用益智健脑颗粒治疗,对照组22例采用脑复康治疗。结果:治疗组在记忆能力的提高和认知功能的改善方面均明显优于对照组。结论:益智健脑颗粒治疗阿尔茨海默氏病确... 详细信息

目的:观察益智健脑颗粒治疗阿尔茨海默氏病的临床疗效。方法:治疗组20例采用益智健脑颗粒治疗,对照组22例采用脑复康治疗。结果:治疗组在记忆能力的提高和认知功能的改善方面均明显优于对照组。结论:益智健脑颗粒治疗阿尔茨海默氏病确有明显的疗效。

关键词：阿尔茨海默氏病/中医药疗法 @益智健脑颗粒