Treating Alzheimer's disease with Yizhijiannao granules by regulating expression of multiple proteins in temporal lobe

作     者：Hong Zhu Liuyang Luo Sihang Hu Keli Dong Guangcheng Li Ting Zhang 

作者机构：Department of Traditional Chinese Medicine Third Xiangya Hospital of Central South University Department of Emergency Bao-an District Hospital of Traditional Chinese Medicine 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第13期

页      面：1283-1287页

核心收录：

学科分类：1002[医学-临床医学] 100203[医学-老年医学] 10[医学] 

基　　金：supported by Startup Fund for 125 Scholars of Third Xiangya Hospital the Natural Science Foundation of Hunan Province,No.07JJ5017 

主　　题：nerve regeneration traditional Chinese medicine neurodegeneration Alzheimer'sdisease Yizhijiannao granule mass spectrometry cognition neural regeneration 

摘      要：Yizhijiannao granules have been shown to improve cognitive function in Alzheimer＇s disease patients. The present study sought to explore the mechanisms involved in the cognitive enhancing effects of Yizhijiannao granule. Senescence-accelerated mouse prone 8 mice with learning and memory disorders were intragastrically treated with Yizhijiannao granule for 8 weeks. Mice intragastrically treated with double distilled water for 8 weeks were considered as the control group. 2D gel electrophoresis was used to isolate total protein from the temporal lobe of senescence-accelerated mouse prone 8 mice, and differential protein spots were obtained by mass spectrometry. Thirty-seven differential protein spots were found in the temporal lobe area of both groups. Ten protein spots were identified： high mobility group box 1, dimethylarginine dimethylaminohydrolase-1, nenroglobin, hemoglobin beta adult major chain, peroxiredoxin-6, cofilin-1, flotiUin 1, peptidylprolyl isomerase A, voltage-dependent anion channel-2 and chaperonin containing TCP1, and subunit 2. Among other functions, these proteins are separately involved in the regulation of amyloid beta production, oxidative stress, neuroinflammation, regulation of tau phosphorylation, and regulation of neuronal apoptosis. Our results revealed that Yizhijiannao granule can regulate the expression of various proteins in the temporal lobe of senescence-accelerated mouse prone 8 mice, and may be therapeutically beneficial for the treatment of Alzheimer＇s disease.