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Multimodal label-free microscopy

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Journal of Innovative Optical Health Sciences 2014年第5期7卷 17-38页

作者： Nicolas Pavillon katsumasa Fujita Nicholas Isaac Smith Biophotonics LaboratoryImmunology Frontier Research Center(IFReC Department of Applied Physics Osaka UniversitySuitaOsaka 565-0871Japan

This paper reviews the different multimodal applications based on a large ext ent of label-freeinaging modalities,ranging from linear to nonlinear optics,while also inchuding *** put specific emphasis on multimodal measurements going across the usual boundaries between imaging modalities,whereas most multimodal platforms combine techniquesbased on similar light interactions or similar hardware *** this review,we limitthe scope to focus on applications for biology such as live cells or tissues,since by their nat ure ofbeing alive or fragile,we are often not free to take liberties with the image acquisition times andare forced to gather the maximum amount of information possible at one *** such samples,imaging by a given label-free method usually presents a challenge in obt aining suficient opticalsignal or is limited in terms of the types of observable *** imaging is thenparticularly attractive for these samples in order to maximize the amount of measured *** multimodal imaging is always useful in the sense of acquiring additional infor-mation from additional modes,at times it is possible to attain information that could not bediscovered using any single mode alone,which is the essence of the progress that is possible usinga multimodal approach.

关键词： Optical microscopy nonlinear optics microspectroscopy

Estimating Individual Tree Diameter and Stem Volume Using Airborne LiDAR in Saga Prefecture, Japan

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Open Journal of Forestry 2018年第2期8卷 205-228页

作者： katsumasa Oono Satoshi Tsuyuki Department of Global Agricultural Sciences The University of Tokyo Tokyo Japan Forest & Agriculture Solution Department Asia Air Survey CO. LTD. Kanagawa Japan

Advancements in airborne LiDAR analysis technology have made it possible to quantify forest resource volumes based on individual trees, and such technology may soon replace field surveys. Unlike individual tree detection or tree height measurements, diameter at breast height (DBH) is difficult to determine directly from measured data and is instead estimated indirectly using the correlation between crown size and DBH. Indicators that represent crown size include crown area, surface area, length, and length ratio, and were utilized with tree height as explanatory variables in ten combinations to determine a regression formula. DBH and tree height calculated from the regression formula were applied to an equation to calculate stem volumes of individual trees. Airborne LiDAR measurements were taken using ALS50-II and ALS60 (Leica) at a density of 4 points/m2. An evaluation of the relationship between the regression formulae and DBH estimates indicated that a combination of crown area, tree height, and crown ratio for Japanese cedar, and a combination of crown area and tree height for Japanese cypress, yielded the highest coefficients of determination. The average error and RMSE were 6.9% and 2.38 cm respectively for Japanese cedar, while the corresponding values for Japanese cypress were 8.35% and 2.51 cm. Once the relationship was extended to the stem volumes of individual trees, the average error was 14.4% and RMSE was 0.10 m3 for Japanese cedar. The corresponding values for Japanese cypress were 18.9% and 0.10 m3. These results demonstrate the potential use of airborne LiDAR as a substitute for field surveys.

关键词： Airborne LiDAR Diameter at Breast Height Individual Treevolume Forest Resource Individual-Tree-Based Approach

从科研到临床:人类肢体再生学的研究趋势和展望

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中华实验外科杂志 2023年第7期40卷 1233-1236页

作者：李涛陈振兵于玲阎明全 Lindsay A.Dawson Ken muneoka 华中科技大学同济医学院附属协和医院手外科武汉430022 美国得州农工大学兽医和生物医学学院生理和药物学系美国得州77843

特殊种类动物的肢体再生是一个科学现象、也是一个科学的事实,以蝾螈为代表的两栖爬行类动物展现了“完全性”的肢体再生能力,以小鼠为代表的啮齿类哺乳动物展现了“节段性”的肢体再生能力,对这些类动物的比较研究有助于早日解密肢体... 详细信息

特殊种类动物的肢体再生是一个科学现象、也是一个科学的事实,以蝾螈为代表的两栖爬行类动物展现了“完全性”的肢体再生能力,以小鼠为代表的啮齿类哺乳动物展现了“节段性”的肢体再生能力,对这些类动物的比较研究有助于早日解密肢体再生的机制和规律,同时有助于发现高等哺乳类动物肢体再生能力受限的原因。虽然人类肢体再生的能力很弱,但仍然展示了一些珍贵的再生现象,近年来在伤口修复、骨延长等领域展示了较好的再生修复的临床应用。伤口炎性反应被认为是肢体再生中与胚芽的形成最为密切相关的因素。建立良好的动物模型来理解伤口与再生诱导反应的关系非常重要。本文作者长期从事肢体外伤临床修复,也长期参与肢体再生的科学研究,2014、2015年在美国德州农工大学Kenmuneoka实验室进行访问学者工作期间建立了一个新的伤口诱导再生的小鼠模型,即在小鼠P3趾尖侧方打孔,由伤口诱导、没有截肢便能成功诱导出胚芽样的再生结构,其内含有大量增生活跃的细胞,重现了小鼠P3趾尖由蜕变到完全增生的整个再生过程,预示了胚芽发育的重要细胞学基础和分子学机制。展望未来,肢体再生领域会有更多的基础突破,将带来更多、更好的临床治疗新技术,不但应用在伤口修复,还会应用于人类骨、关节、血管、神经、肌腱、皮肤等器官的再生,并能促进更多的再生医学转化。

关键词：肢体再生伤口修复发育

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Relationship among Eating Behavior, Effortful Control, and Working Memory in Female Young Adults

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Health 2016年第12期8卷 1187-1194页

作者： katsumasa Momoi Kumiko Ohara Yoshimitsu Okita Tomoki Mase Chiemi Miyawaki Tomoko Fujitani Harunobu Nakamura Graduate School of Human Development and Environment Kobe University Kobe Japan Faculty of Health and Welfare Tokushima Bunri University Tokushima Japan Graduate School of Engineering Shizuoka University Hamamatsu Japan Faculty of Human Development and Education Kyoto Women’s University Kyoto Japan Department of Early Childhood Education Heian Jogakuin (St. Agnes’) College Kyoto Japan

The management of eating behavior plays an important role in health maintenance. In this study, we investigated the relationship between eating behavior and effortful control in female young adults. Participants completed the questionnaire measures of effortful control and eating behaviors and Stroop cognitive interference task. The results showed that restrained eating was positively correlated with activation control;emotional eating was negatively correlated with inhibitory control and attentional control;external eating was negatively correlated with inhibitory, activation, and attentional control. The scores for activation control and restrained eating were higher for participants with a low Stroop error rate than for those with a high Stroop error rate. These results indicate that restrained eating has a different association with effortful control than doing emotional and external eating.

关键词： Executive Function Working Memory Effortful Control Eating Behavior

Effect of a combination of astaxanthin supplementation, heat stress, and intermittent reloading on satellite cells during disuse muscle atrophy

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第11期19卷 844-852页

作者： Toshinori YOSHIHARA Takao SUGIURA Nobuyuki MIYAJI Yuki YAMAMOTO Tsubasa SHIBAGUCHI Ryo KAKIGI Hisashi NAITO katsumasa GOTO Daijiro OHMORI Toshitada YOSHIOKA Graduate School of Health and Sports Science Juntendo UniversityInzaiChiba 270-1695Japan Faculty of Education Yamaguchi UniversityYamaguchiYamaguchi 753-8513Japan Toyo Koso Kagaku Co.Ltd. UrayasuChiba 279-0041Japan Institute of Health and Sports Science Kurume UniversityKurumeFukuoka 839-8502Japan Institute of Liberal Arts and Science Kanazawa UniversityKanazawaIshikawa 920-1192Japan Faculty of Medicine Juntendo UniversityBunkyo-kuTokyo 113-0033Japan Graduate School of Health Sciences Toyohashi SOZO UniversityToyohashiAichi 440-8511Japan Department of Chemistry School of MedicineJuntendo UniversityInzaiChiba 270-1695Japan Hirosaki Gakuin University HirosakiAomori 036-8577Japan

We examined the effect of a combination of astaxanthin （AX） supplementation, repeated heat stress, and intermittent reloading （IR） on satellite cells in unloaded rat soleus muscles. Forty-nine male Wistar rats （8-week-old） were divided into control, hind-limb unweighting （HU）, IR during HU, IR with AX supplementation, IR with repeated heat stress （41.0-41.5 ℃ for 30 min）, and IR with AX supplementation and repeated heat stress groups. After the experimental period, the antigravitational soleus muscle was analyzed using an immunohistochemical technique. Our results revealed that the combination of dietary AX supplementation and heat stress resulted in protection against disuse muscle atrophy in the soteus muscle. This protective effect may be partially due to a higher satellite cell number in the atrophied soleus muscle in the IR/AX/heat stress group compared with the numbers found in the other groups. We concluded that the combination treatment with dietary AX supplementation and repeated heat stress attenuates soleus muscle atrophy, in part by increasing the number of satellite cells.

关键词： Antioxidant astaxanthin Disuse muscle atrophy Heat stress Satellite cell

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风障在北海道Tokachi地区的重要作用

中国水土保持 2002年第7期 13-13页

作者： Osamu Tsuji Toshimi muneoka Ken Fujiwara Obihiro Uni.of Agri.and Veterinary Medicine Inada-choObihiroHokkaidoObihiro Uni.of Agri.and Veterinary MedicineInada-choObihiroHokkaidoObihiro Uni.of Agri.and Veterinary MedicineInada-choObihiroHokkaido Japan0808555Japan0808555Japan0808

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风障在北海道Tokachi地区的重要作用

中国水土保持 2002年第7期 17-17页

作者： Osamu Tsuji,Toshimi muneoka,Ken Fujiwara Obihiro Uni.of Agri.and Veterinary Medicine Inada-cho Obihiro Hokkaido Obihiro Uni.of Agri.and Veterinary Medicine Inada-cho Obihiro Hokkaido Obihiro Uni.of Agri.and Veterinary Medicine Inada-cho Obihiro Hokkaido Japan 0808555 Japan 0808555 Japan 0808

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SHIROIYA酒店

现代装饰 2021年第3期 120-127页

作者： Sou Fujimoto katsumasa Tanaka(摄影) Shinya Kigure(摄影) 舒禹(编译) Sou Fujimoto Architects

该项目旨在翻修前桥市中心的一栋被称为"遗产塔"的四层钢筋混凝土主楼建筑。设计师的第二项任务是建造新的大楼,称为"绿塔"。自江户时代以来,主楼内的"白屋旅馆"已有300多年的历史,旅馆关闭后使得这座建筑空置。设计师在考察现场后,决... 详细信息

该项目旨在翻修前桥市中心的一栋被称为"遗产塔"的四层钢筋混凝土主楼建筑。设计师的第二项任务是建造新的大楼,称为"绿塔"。自江户时代以来,主楼内的"白屋旅馆"已有300多年的历史,旅馆关闭后使得这座建筑空置。设计师在考察现场后,决定拆除四层楼的墙壁与地板,创建一个巨大的中庭。

关键词：主楼建筑钢筋混凝土中庭江户时代酒店旅馆设计师地板

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The Effect of Treatment Position on Rectal and Bladder Dose-Volume Histograms for Prostate Radiotherapy Planned with 3-Dimensional Conformal Radiotherapy, Intensity-Modulated Radiotherapy and Volumetric Modulated Arc Therapy

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2014年第2期3卷 88-97页

作者： Kotaro Terashima katsumasa Nakamura Tomonari Sasaki Saiji Ohga Tadamasa Yoshitake Kazushige Atsumi Makoto Shinoto Kaori Asai Keiji Matsumoto Hidenari Hirata Yoshiyuki Shioyama Akihiro Nishie Hiroshi Honda Department of Clinical Radiology Graduate School of Medical Sciences Kyushu University Fukuoka Japan Department of Radiology Kyushu University Hospital at Beppu Beppu Japan Kyushu International Heavy Ion Beam Therapy Center Tosu Saga Japan

Purpose: To compare target coverage and organ at risk (OAR) sparing in the supine and prone positions with 3-dimensional conformal radiotherapy (3DCRT), intensity-modulated radiotherapy (IMRT) and volumetric modulated arc therapy (VMAT) in low- and high-risk prostate radiotherapy cases. Materials and Methods: Using magnetic resonance images of five healthy volunteers, six treatment plans (supine 3DCRT, prone 3DCRT, supine IMRT, prone IMRT, supine VMAT and prone VMAT) were generated. Planning target volume 1 (PTV1) was defined as the prostate gland plus the seminal vesicles with adequate margins in a high-risk setting, while PTV2 was defined as prostate only with margins in a low-risk setting. The mean dose for both PTV1 and PTV2 was set at 78 Gy. Plans generated by each of the 3 techniques were compared between the supine and prone positions using dose-volume histograms (DVHs). Results: For PTV1, prone 3DCRT provided a significantly higher D98% than did supine 3DCRT, and its homogeneity index (HI) was significantly better. IMRT and VMAT values did not differ significantly between the prone and supine positions. For PTV2, no values differed significantly between the supine and prone positions under any treatment plan. With respect to OAR, the rectal D mean, D2%, V50, and V60 values of PTV1 were statistically higher in supine 3DCRT than in prone 3DCRT, while there were no significant differences in rectal values between the supine and prone positions with IMRT or VMAT. The rectal Dmean, V50, V60, V70, and V75 values of prone 3DCRT were significantly higher than those of supine IMRT or supine VMAT. There were no significant differences in any values for the rectum and bladder for PTV2. Conclusion: Although prone 3DCRT was found to be superior to supine 3DCRT in terms of rectal sparing in high-risk prostate cancer, IMRT and VMAT techniques could possibly cover this disadvantage.

关键词： Prostate Radiotherapy Dosimetric Comparison 3DCRT IMRT VMAT