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improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls

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Frontiers of Environmental Science & Engineering 2021年第2期15卷 33-42页

作者： karla ilićđurđić Raluca Ostafe Olivera Prodanović AleksandraĐurđevićĐelmaš Nikolina Popović Rainer Fischer Stefan Schillberg Radivoje Prodanović University of Belgrade-Faculty of Chemistry Studentski trg 12-1611000 BelgradeSerbia Molecular Evolution Protein Engineering and Production facility(MEPEP) Purdue UniversityWest LafayetteIN 47907USA institute of Molecular Biotechnology RWTH Aachen University52074 AachenGermany institute for Multidisciplinary Research Kneza Višeslava 111030 BelgradeSerbia Departments of Biological Sciences and Chemistry Purdue UniversityWest LafayetteIN 47907USA Fraunhofer institute for Molecular Biology and Applied Ecology iME 52074 AachenGermany

The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation *** peroxidase(LiP)from Phanerochaete chrysosporium is a hemecontaining lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules,including industrial *** enzyme is therefore ideal as a starting point for protein ***,we subjected two positions(165 and 264)in the environment of the catalytic Trp171 residue to saturation mutagenesis,and the resulting library of 104 independent clones was expressed on the surface of yeast *** yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more *** identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes(Evans blue,amido black 10B and Guinea green)and up to 13-fold higher catalytic ***,cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis,achieving significant increases in both enzyme activity and stability compared to a whole-cell *** cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 *** best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

关键词： Bioremediation Enzyme immobilization Protein engineering Yeast surface display

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improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalytic pocket and the application of peroxidase-coated yeast cell walls

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Frontiers of Environmental Science & Engineering 2021年第2期 14-23页

作者： karla ili? ?ur?i? Raluca Ostafe Olivera Prodanovi? Aleksandra ?ur?evi? ?elma? Nikolina Popovi? Rainer Fischer Stefan Schillberg Radivoje Prodanovi? University of Belgrade-Faculty of Chemistry Molecular Evolution Protein Engineering and Production facility (MEPEP) Purdue University institute of Molecular Biotechnology RWTH Aachen University institute for Multidisciplinary Research Departments of Biological Sciences and Chemistry Purdue University Fraunhofer institute for Molecular Biology and Applied Ecology iME

The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation *** peroxidase(LiP) from Phanerochaete chrysosporium is a hemecontaining lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules,including industrial *** enzyme is therefore ideal as a starting point for protein ***,we subj ected two positions(165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis,and the resulting library of 10～4 independent clones was expressed on the surface of yeast *** yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more *** identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes(Evans blue,amido black 10 B and Guinea green) and up to 13-fold higher catalytic ***,cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis,achieving significant increases in both enzyme activity and stability compared to a whole-cell *** cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 *** best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

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Predicting adolescent perfectionism: The role of socio-demographic traits, personal relationships, and media

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World Journal of Clinical Cases 2022年第1期10卷 189-204页

作者： Goran Livazović karla Kuzmanović Department of Pedagogy University of J.J.StrossmayerOsijek 31000Osječko-baranjskaCroatia Study Programme in Pedagogy Faculty of Humanities and Social SciencesOsijek 31000Osječko-baranjskaCroatia

BACKGROUND The present study investigated perfectionism from the bioecological model perspective as a multidimensional construct manifested in forms of excessively high personal standards,exaggerated worries about personal mistakes,doubt in one’s performance,oversized order and organization emphasis,and the importance of parental valuations and *** To investigate the relation between perfectionism;the quality of family,peer,and college relationships;and media usage and content *** The research was implemented in 2020 with 203 students(134 female,66%)aged 18-25 years,enrolled at the University of Osijek in *** questionnaire had five parts:A)sociodemographic data;B)the Multidimensional Perfectionism Scale;C)the“general life satisfaction”and“current situational life satisfaction”scale;D)family,peer,and college relationships quality scale;and E)media usage and content interests *** of the implemented instruments showed satisfactory reliability.A hierarchical regression analysis was implemented with the aim of establishing significant perfectionism *** Age and gender were significant predictors of *** with lower family relationship quality reported higher parental expectations and complaining as well as significantly higher doubts in personal performance and concern about ***,a lower peer relationship quality predicted doubts in personal performance and stronger concerns about *** quality of college relationships positively predicted higher perfectionist personal standards and *** life satisfaction predicted higher concerns about mistakes,while current situational life satisfaction predicted higher levels of perfectionist *** usage intensity had no significant *** interest in information-educational media predicted higher personal standards as well as concern about mistakes and *** interests in entertainment

关键词： Perfectionism Family Peers Colleagues Life satisfaction Media content

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Tribological performance of uncoated and TiCN-coated D2,M2 and M4 steels under lubricated condition

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Journal of iron and Steel Research international 2017年第8期24卷 823-829页

作者： Luis Daniel Aguilera Camacho Santos García Miranda karla Judith Moreno instituto Tecnologico de Celaya/TNM Apartado Postal 5738010-CelayaGuanajuatoMexico

Hard coatings are used to improve the wear resistance of metals which largely depends on adhesion between substrate and *** wear and friction behavior of uncoated and TiCN-coated D2,M2 and M4 steels were evaluated by apin-on-disk test under lubricated *** order to evaluate the influence of lubricant on wear performance,dry friction tests were also *** results showed that friction coefficients were very similar for both uncoated and TiCN-coated *** lubricated conditions,the uncoated D2 tool steel exhibited the lowest friction coefficient,but the TiCN-coated D2 steel presented the smallest wear *** was the main wear mechanism in all the ***,microhardness measurements were carried out,finding an influence of the steel substrate on the hardness of the ***,adhesion test was conducted,suggesting agood adhesion of class 1 between substrates and TiCN coatings.

关键词： D2 tool steel M2 high speed steel M4 high speed steel TiCN coating Friction Wear

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Biocomposite Films of Polylactic Acid Reinforced with Microcrystalline Cellulose from Pineapple Leaf Fibers

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Journal of Renewable Materials 2019年第1期7卷 9-20页

作者： Galia Moreno karla Ramirez Marianelly Esquivel Guillermo Jimenez Laboratory of Polymers(POLiUNA) School of ChemistryUniversidad NacionalHerediaCosta Rica

Poly(lactic acid)(PLA)composite films reinforced with microcrystalline cellulose(MCC)extracted from pineapple leaf fibers(PALF)were prepared by a solution casting *** an attempt to improve the interaction between PLA and cellulose,two approaches were adopted;first,poly(ethylene glycol)(PEG)was used as a surfactant,and second,the cellulosic fibers were pre-treated using tert-butanol(TBA).Lignocellulosic and cellulosic substrates were characterized using Fourier transform infrared(FTiR),wide-angle X-ray scattering(WAXS),and thermogravimetrical analysis(TGA).MCC from PALF showed good thermal stability,left few residues after decomposing,and exhibited high crystallinity ***,thermal and thermomechanical properties of the PLA composites were also *** PLA endotherms were observed in composites with TBA-treated MCC due to crystal nucleation *** ultimate tensile strain values for all composites were lower than that of the pristine ***,4 wt.%MCC content provided balanced engineering properties in terms of static and dynamic tensile properties.

关键词： Cellulose composites microcrystalline PLA pineapple

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isolation and Characterization of Nanocellulose Obtained from industrial Crop Waste Resources by Using Mild Acid Hydrolysis

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Journal of Renewable Materials 2018年第4期6卷 362-369页

作者： Galia Moreno karla Ramirez Marianelly Esquivel Guillermo Jimenez Laboratory of Polymers(POLiUNA) School of ChemistryNational UniversityHerediaCosta Rica

Cellulose,microcrystalline cellulose and nanocellulose were prepared from three agricultural waste resources:pineapple leaf(PALF),banana rachis(BR),and sugarcane bagasse(SCB).Each waste resource was first converted into microcrystalline cellulose which was subsequently converted into cellulose nanoparticles by using mild(30%w/v)and strong(60%w/v)sulfuric acid concentrations for *** transform infrared spectroscopy(FTiR),X-ray diffraction(XRD),and thermogravimetric analysis(TGA)were used to characterize each waste resource and extracted cellulosic ***,nanocelluloses were studied by zeta potential,size analysis,and transmission electron microscopy(TEM).Cellulose nanowhiskers were successfully obtained and isolated with a 33%average yield by applying a mild acid *** BR and SCB proved to be more promising agricultural waste resources in terms of their crystalline cellulosic content and properties.

关键词： Cellulose pineapple leaf sugarcane bagasse banana rachis whiskers

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Challenges to the early diagnosis and treatment of breast cancer in developing countries

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World Journal of Clinical Oncology 2014年第3期5卷 465-477页

作者： karla Unger-Salda?a Economy Division Centro de Investigación y Docencia Económicas (CIDE) 01210 Mexico CityMéxico

This critical review of the literature assembles and compares available data on breast cancer clinical stage, time intervals to care, and access barriers in different countries. it provides evidence that while more than 70% of breast cancer patients in most high-income countries are diagnosed in stages Ⅰ and Ⅱ, only 20%-50% patients in the majority of low- and middleincome countries are diagnosed in these earlier stages. Most studies in the developed world show an association between an advanced clinical stage of breast cancer and delays greater than three months between symptom discovery and treatment start. The evidence assembled in this review shows that the median of this interval is 30-48 d in high-income countries but 3-8 mo in low- and middle-income countries. The longest delays occur between the first medical consultation and the beginning of treatment, known as the provider interval. The little available evidence suggests that access barriers and quality deficiencies in cancer care are determinants of provider delay in low- and middle-income countries. Research on specific access barriers and deficiencies in quality of care for the early diagnosis and treatment of breast cancer is practically non-existentin these countries, where it is the most needed for the design of cost-effective public policies that strengthen health systems to tackle this expensive and deadly disease.

关键词： Breast cancer Early diagnosis Delays Time intervals Clinical stage Access Health care delivery

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Bird habitat preferences drive hemoparasite infection in the Neotropical region

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integrative Zoology 2021年第5期16卷 755-768页

作者： Gabriel Massaccesi DE LA TORRE karla Magalhães CAMPiÃO Biological interactions Departamento de ZoologiaUniversidade Federal do ParanáCuritibaBrazil Programa de Pós-graduação em Ecologia e Conservação Universidade Federal do ParanáCuritibaBrazil

The role that the environment plays in vector-borne parasite infection is one of the central factors for understanding disease *** assessed how Neotropical bird foraging strata and habitat preferences determine infection by parasites of the genera Haemoproteus,Plasmodium,Leucocytozoon,and Trypanosoma and filarioids,and tested for phylogenetic signal in these host-parasite *** performed extensive searches of the scientific literature and created a database of hemoparasite *** collected data on host body mass,foraging strata,habitat preference,and migratory status,and tested if host ecological traits predict each hemoparasite occurrence and prevalence using a phylogenetic Bayesian *** of Plasmodium tend to infect birds from tropical forests while birds from altitudinal environments are likely to be infected by species of *** probability of a bird being infected by filarioid or Trypanosoma is higher in lowland *** species that occur in anthropic environments and dry habitats of tropical latitudes are more susceptible to infection by species of *** foraging strata is also influential and bird species that forage in the mid-high and canopy strata are more prone to infection by species of Haemoproteus and *** also identified phylogenetic signal for host-parasite associations with the probability of infection of Neotropical birds by any hemoparasite being more similar among more closely related *** provided a useful framework to identify environments that correlate with hemoparasite infection,which is also helpful for detecting areas with potential suitability for hemoparasite infection due to land conversion and climate change.

关键词： habitat use haemosporidians host-parasite interaction migratory birds prevalence

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Survival and comorbidities in lung cancer patients:Evidence from administrative claims data in Germany

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Oncology Research 2022年第4期30卷 173-185页

作者： DiEGO HERNANDEZ CHiH-YUAN CHENG karla HERNANDEZ-ViLLAFUERTE MiCHAEL SCHLANDER Division of Health Economics German Cancer Research Center(DKFZ)HeidelbergGermany Medical Faculty Mannheim Heidelberg UniversityMannheimGermany

Lung cancer is the most common cancer type worldwide and has the highest and second highest mortality rate for men and women respectively in ***,the role of comorbid illnesses in lung cancer patient prognosis is still *** analyzed administrative claims data from one of the largest statutory health insurance(SHi)funds in Germany,covering close to 9 million people(11%of the national population);observation period was from 2005 to *** cancer patients and their concomitant diseases were identified by iCD-10-GM *** were classified according to the Charlson Comorbidity index(CCi).incidence,comorbidity prevalence and survival are estimated considering sex,age at diagnosis,and place of *** Meier curves with 95%confidence intervals were built in relation to common *** identified 70,698 lung cancer incident cases in the *** and survival figures are comparable to official statistics in *** prevalent comorbidities are chronic obstructive pulmonary disease(COPD)(36.7%),followed by peripheral vascular disease(PVD)(18.7%),diabetes without chronic complications(17.4%),congestive heart failure(CHF)(16.5%)and renal disease(14.7%).Relative to overall survival,lung cancer patients with CHF,cerebrovascular disease(CEVD)and renal disease are associated with largest drops in survival probabilities(9%or higher),while those with PVD and diabetes without chronic complications with moderate drops(7%or lower).The study showed a negative association between survival and most common comorbidities among lung cancer patients,based on a large sample for *** research needs to explore the individual effect of comorbidities disentangled from that of other patient characteristics such as cancer stage and histology.

关键词： Lung Cancer Comorbidities Survival Administrative data Statutory health insurance

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induced pluripotent stem cells throughout the animal kingdom:Availability and applications

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World Journal of Stem Cells 2019年第8期11卷 491-505页

作者： Lais Vicari de Figueiredo Pessoa Fabiana Fernandes Bressan Kristine karla Freude Group of Stem Cell Models for Studies of Neurodegenerative Diseases Section for Pathobiological Sciences Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Frederiksberg 1870 Denmark Department of Veterinary Medicine Faculty of Animal Science and Food Engineering University of Sao Paulo Pirassununga 13635-000 Sao Paulo Brazil

Up until the mid 2000s, the capacity to generate every cell of an organism was exclusive to embryonic stem cells. in 2006, researchers Takahashi and Yamanaka developed an alternative method of generating embryonic-like stem cells from adult cells, which they coined induced pluripotent stem cells (iPSCs). Such iPSCs possess most of the advantages of embryonic stem cells without the ethical stigma associated with derivation of the latter. The possibility of generating “custom-made” pluripotent cells, ideal for patient-specific disease models, alongside their possible applications in regenerative medicine and reproduction, has drawn a lot of attention to the field with numbers of iPSC studies published growing exponentially. iPSCs have now been generated for a wide variety of species, including but not limited to, mouse, human, primate, wild felines, bovines, equines, birds and rodents, some of which still lack well-established embryonic stem cell lines. The paucity of robust characterization of some of these iPSC lines as well as the residual expression of transgenes involved in the reprogramming process still hampers the use of such cells in species preservation or medical research, underscoring the requirement for further investigations. Here, we provide an extensive overview of iPSC generated from a broad range of animal species including their potential applications and limitations.

关键词： Pluripotency Embryonic Stem cell Reprogramming Animal Wild induced pluripotency

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