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Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX

Synthetic and Systems Biotechnology 2017年第4期2卷 310-319页

作者： Andreas Domrose Robin Weihmann Stephan Thies karl-erich jaeger Thomas Drepper Anita Loeschcke Institute of Molecular Enzyme Technology Heinrich Heine University DüsseldorfForschungszentrum JülichJülichGermany Bioeconomy Science Center(BioSC) Forschungszentrum JülichJülichGermany Institute of Bio-and Geosciences(IBG-1) Forschungszentrum JülichJülichGermany

Microbial secondary metabolites represent a rich source of valuable compounds with a variety of applications in medicine or *** exploitation of this wealth of chemicals requires the functional expression of the respective biosynthetic genes in amenable heterologous *** have previously established the TREX system which facilitates the transfer,integration and expression of biosynthetic gene clusters in various bacterial ***,we describe the yTREX system,a new tool adapted for one-step yeast recombinational cloning of gene *** show that with yTREX,Pseudomonas putida secondary metabolite production strains can rapidly be constructed by random targeting of chromosomal promoters by Tn5 *** of this approach was corroborated by prodigiosin production after yTREX cloning,transfer and expression of the respective biosynthesis genes from Serratia ***,the applicability of the system for effective pathway rerouting by gene cluster adaptation was demonstrated using the violacein biosynthesis gene cluster from Chromobacterium violaceum,producing pathway metabolites violacein,deoxyviolacein,prodeoxyviolacein,and *** producing both prodigiosin and violaceins could be readily identified among clones obtained after random chromosomal integration by their strong ***,the addition of a promoter-less reporter gene enabled facile detection also of phenazine-producing clones after transfer of the respective phenazine-1-carboxylic acid biosynthesis genes from Pseudomonas *** compounds accumulated to substantial titers in the mg *** thus corroborate here the suitability of *** for the biosynthesis of diverse natural products,and demonstrate that the yTREX system effectively enables the rapid generation of secondary metabolite producing bacteria by activation of heterologous gene clusters,applicable for natural compound discovery and combinatorial biosy

关键词： Synthetic biology Yeast recombinational cloning Tn5 transposition Heterologous gene cluster expression Secondary metabolite production Pseudomonas putida

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*** GDSL外膜酯酶对细胞运动性的影响

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第六届中国酶工程学术研讨会

作者：王建军 Susanne Wilhelm Frank Rosenau karl-erich jaeger 中国科学院微生物研究所微生物资源国家重点实验室 Institut fr Molekulare Enzymtechnologie Heinrich-Heine-Universitt DsseldorfForschungszentrum JlichD-52426 JlichGermany

本研究在E coli BL21(DE3)和Xanthomonas campestris野生菌株中克隆和表达了GDSL酯酶Xc est,并且在Xcst信号肽之后融合了E tag基因,融合基因在野生菌株中获得表达。融合表达细胞进行细胞组分分离,蛋白酶处理和Western blot检测,以上结...

本研究在E coli BL21(DE3)和Xanthomonas campestris野生菌株中克隆和表达了GDSL酯酶Xc est,并且在Xcst信号肽之后融合了E tag基因,融合基因在野生菌株中获得表达。融合表达细胞进行细胞组分分离,蛋白酶处理和Western blot检测,以上结果结合激光共聚焦显微镜检测证明,此酶是外膜蛋白,其beta结构域插入了细胞外膜。运动性实验证明,与野生型相比,突变体的游动能力被延迟,涌动能力完全消失。Xanthomonas campestris属于植物病原菌,此病菌的流行可以造成巨大的农业损失。Xanthomonas campestris属于变形菌门的γ亚类,共有141种致病株,每一个致病株都有对应的宿主。对Xanthomonascampestris的研究集中于其治病因子和由此引起的植物疾病,以及其产生黄原胶及生物催化酶类的能力。到目前为止,已经有三株Xanthomonas campestris获得了全基因组测序,这些基因组中都标注了GDSL,酯酶。GDSL酯酶属于酯水解酶的第二家族。此家族具有明显的GDSL特征序列,此序列同真酯酶的GxSxG不同。GDSL序列位于蛋白的N端,真酯酶的GxSxG序列一般位于蛋白中心。GDSL酯酶的C端和最近发现的自转移体致病因子的结构非常相似。GDSL酯酶的催化机理目前还不是很清楚。研究表明,一种来源于E coli的GDSL,巯基酯酶TEP-Ⅰ具有高柔韧性的活性中心结构以及底物结合区域。GDSL酯酶的催化机理很有可能遵循‘锁与钥匙’的机理。这一机理可以很好的解释为什么TEP-I具有蛋白酶,巯基酯酶,芳基酯酶和溶血磷脂酶多重酶活力。系统进化分析表明,GDSL,酯酶分为5个进化分支,多数细菌来源的GDSL酯酶属于第一和第二进化分支,高等植物来源的GDSL,酯酶属于其他三个进化分支。最近的研究表明,GDSL酯酶在AHL合成的过程中起到了重要作用。AHL是革兰氏阴性菌群体感应中的可扩散信号分子,群体感应控制细胞的很多形状表型,例如生物膜形成,治病因子合成等。目前Xanthomonas yesicatoria DSM 50861的GDSL酯酶已经在大肠杆菌中获得了表达。此酶对于短链和苯基对位取代的底物具有较高水解活力。虽然此酶不能作用于长链脂肪酸的三甘油酯,但是可以水解长链的酯。在本文中,GDSL,酯酶在大肠杆菌及野生黄单胞菌中获得表达,GDSL酯酶缺失突变体的运动性与野生型相比具有较大的差异。

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Risk,Rationality,and Resilience

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International Journal of Disaster Risk Science 2010年第1期1卷 10-16页

作者： Carlo jaeger Potsdam Institute for Climate Impact Research(PIK) 14473 PotsdamGermany

Improving our ability to cope with large risks is one of the key challenges for humankind in this century. This article outlines a research program in this perspective. Starting with a concrete example of a relatively small disaster, it questions simplistic ideas of rationality. It then proposes a fresh look at the concepts of probability and utility in the context of socioecological systems. This leads first to an emphasis on the problem of equilibrium selection, and then to a distinction between three kinds of resilience that matter both for theory and practice of risk management. They can be investigated by paying attention to the transitions into and out of actual disasters.

关键词： Resilience management. disaster

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A Fast Ambient Occlusion Method for Real-Time Plant Rendering

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Journal of Computer Science & Technology 2007年第6期22卷 859-866页

作者：滕军 Marc jaeger 胡包钢 LIAMA/NLPR Institute of AutomationChinese Academy of Sciences INRIA-Futurs DigiPlante TeamCIRAD AMAPMontpellierFrance

Global illumination effects are crucial for virtual plant rendering. Whereas real-time global illumination rendering of plants is impractical, ambient occlusion is an efficient alternative approximation. A tree model with millions of triangles is common, and the triangles can be considered as randomly distributed. The existing ambient occlusion methods fail to apply on such a type of object. In this paper, we present a new ambient occlusion method dedicated to real time plant rendering with limited user interaction. This method is a three-step ambient occlusion calculation framework which is suitable for a huge number of geometry objects distributed randomly in space. The complexity of the proposed algorithm is O（n）, compared to the conventional methods with complexities of O（n^2）. Furthermore, parameters in this method can be easily adjusted to achieve flexible ambient occlusion effects. With this ambient occlusion calculation method, we can manipulate plant models with millions of organs, as well as geometry objects with large number of randomly distributed components with affordable time, and with perceptual quality comparable to the previous ambient occlusion methods.

关键词： accessibility value estimation plant visualization real-time rendering

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Editorial Note on the 10-Year Anniversary of the International Journal of Disaster Risk Science:A Thank You Letter

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International Journal of Disaster Risk Science 2020年第4期11卷 411-413页

作者： Peijun Shi Carlo jaeger Academy of Disaster Reduction and Emergency Management Ministry of Emergency Management and Ministry of EducationFaculty of Geographical ScienceBeijing Normal UniversityBeijing 100875China Global Climate Forum 10178 BerlinGermany

This year marks the 10-year anniversary of the International Journal of Disaster Risk Science(IJDRS).On this occasion,as Editors-in-Chief,we are deeply grateful for the support and contribution of many who have enable... 详细信息

关键词： journal contributing deeply

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Choice for China:What Role for Vocational Education in Green Growth?

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China & World Economy 2014年第5期22卷 55-75页

作者： Carlo jaeger Beijing Normal University

Green growth cannot succeed without significant changes in the education system and the closely related social division of labor. This paper combines historical evidence and a game-theoretic analysis to study the relation between vocational education and green growth. It is found that a low-vocation and a high-vocation equilibrium can be distinguished in the interplay between education and labor markets, and that a high-vocation equilibrium is better suited for green growth. A t the present stage of development, there are tendencies in both directions in China. Therefore, China has the possibility to successfully implement a green growth strategy by developing a strong vocational education with Chinese characteristics.

关键词： China green growth vocational education

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The Direct Contribution of Astrocytes and Microglia to the Pathogenesis of Hepatic Encephalopathy

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Journal of Clinical and Translational Hepatology 2019年第4期7卷 352-361页

作者： Victoria jaeger Sharon DeMorrow Matthew McMillin Baylor Scott&White Health Department of Internal MedicineTempleTXUSA Texas A&M University Health Science Center Department of Medical PhysiologyTempleTXUSA Central Texas Veterans Health Care System TempleTXUSA University of Texas at Austin Dell Medical SchoolDepartment of Internal MedicineAustinTXUSA University of Texas at Austin College of PharmacyAustinTXUSA

Hepatic encephalopathy is a neurological complication result-ing from loss of hepatic function and is associated with poor clinical *** acute liver failure over 20%of mortality can be associated with the development of hepatic *** patients with liver cirrhosis,1-year sur-vival for those that develop overt hepatic encephalopathy is under 50%.The pathogenesis of hepatic encephalopathy is complicated due to the multiple disruptions in homeostasis that occur following a reduction in liver *** these,elevations of ammonia and neuroinflammation have been shown to play a significant contributing role to the develop-ment of hepatic *** of the urea cycle following liver dysfunction leads to elevations of circulating ammonia,which enter the brain and disrupt the functioning of *** results in dysregulation of metabolic path-ways in astrocytes,oxidative stress and cerebral *** ammonia,circulating chemokines and cytokines are increased following liver injury,leading to activation of micro-glia and a subsequent neuroinflammatory *** combination of astrocyte dysfunction and microglia activation are significant contributing factors to the pathogenesis of hepatic encephalopathy.

关键词： Acute liver failure Microglia Neuroinflammation Ammonia Astroglia

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Systemic Risks: A Homomorphic Approach on the Basis of Complexity Science

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International Journal of Disaster Risk Science 2018年第3期9卷 292-305页

作者： Klaus Lucas Ortwin Renn Carlo jaeger Saini Yang Institute of Technical Thermodynamics Aachen University Institute for Advanced Sustainability Studies (IASS) Economic and Social Science Department University of Stuttgart Academy of Disaster Reduction and Emergency Management School of Geography Beijing Normal University Global Climate Forum Key Laboratory of Environmental Change and Natural Disasters Ministry of Education Beijing Normal University

Although the notion of systemic risk gained prominence with respect to financial systems, it is a generic term that refers to risks of increasing importance in many domains—risks that cannot be tackled by conventional techniques of risk management and governance. We build on a domain-overarching definition of systemic risks by highlighting crucial properties that distinguish them from conventional risks and plain disasters. References to typical examples from various domains are included. Common features of systemic risks in different domains—such as the role of agents and emergence phenomena, tipping and cascading, parameters indicating instability, and historicity—turn out to be more than noncommittal empirical observations. Rather these features can be related to fundamental theory for relatively simple and well-understood systems in physics and chemistry. A crucial mechanism is the breakdown of macroscopic patterns of whole systems due to feedback reinforcing actions of agents on the microlevel, where the reinforcement is triggered by boundary conditions moving beyond critical tipping *** the whole article, emphasis is placed on the role of complexity science as a basis for unifying the phenomena of systemic risks in widely different domains.

关键词： Complexity science Integrated risk governance Multiagent modeling Systemic risks

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Analysis for Effects of Temperature Rise of PV Modules upon Driving Distance of Vehicle Integrated Photovoltaic Electric Vehicles

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Energy and Power Engineering 2024年第4期16卷 131-150页

作者： Masafumi Yamaguchi Yasuyuki Ota Taizo Masuda Christian Thiel Anastasios Tsakalidis Arnulf jaeger-Waldau Kenji Araki Kensuke Nishioka Tatsuya Takamoto Takashi Nakado Kazumi Yamada Tsutomu Tanimoto Yosuke Tomita Yusuke Zushi Kenichi Okumura Takashi Mabuchi Akinori Satou Kyotaro Nakamura Ryo Ozaki Nobuaki Kojima Yoshio Ohshita Toyota Technological Institute Nagoya Japan Faculty of Engineering Miyazaki University Miyazaki Japan Toyota Motor Corporation Susono Japan European Commission— Joint Research Centre Ispra Italy Sharp Corporation Nara Japan Toyota Motor Corporation Toyota Japan Nissan Motor Corporation Yokosuka Japan

The development of vehicle integrated photovoltaics-powered electric vehicles (VIPV-EV) significantly reduces CO2 emissions from the transport sector to realize a decarbonized society. Although long-distance driving of VIPV-EV without electricity charging is expected in sunny regions, driving distance of VIPV-EV is affected by climate conditions such as solar irradiation and temperature rise of PV modules. In this paper, detailed analytical results for effects of climate conditions such as solar irradiation and temperature rise of PV modules upon driving distance of the VIPV-EV were presented by using test data for Toyota Prius and Nissan Van demonstration cars installed with high-efficiency InGaP/GaAs/InGaAs 3-junction solar cell modules with a module efficiency of more than 30%. The temperature rise of some PV modules studied in this study was shown to be expressed by some coefficients related to solar irradiation, wind speed and radiative cooling. The potential of VIPV-EV to be deployed in 10 major cities was also analyzed. Although sunshine cities such as Phoenix show the high reduction ratio of driving range with 17% due to temperature rise of VIPV modules, populous cities such as Tokyo show low reduction ratio of 9%. It was also shown in this paper that the difference between the driving distance of VIPV-EV driving in the morning and the afternoon is due to PV modules’ radiative cooling. In addition, the importance of heat dissipation of PV modules and the development of high-efficiency PV modules with better temperature coefficients was suggested in order to expand driving range of VIPV-EV. The effects of air-conditioner usage and partial shading in addition to the effects of temperature rise of VIPV modules were suggested as the other power losses of VIPV-EV.

关键词： Vehicle Integrated Photovoltaics (VIPV) VIPV-Powered Electric Vehicles Driving Distance PV Modules Solar Irradiation Temperature Rise Radiative Cooling

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工业应用中的新生物催化剂

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生物技术世界 2007年第1期4卷 43-44页

作者： MonikaKonarzycka-Bessler karl-erich jaeger 李升伟（译）

应用各种酶进行化学化合物的生物技术生产被人们称为“白色生物技术”，许多化学工业公司应用此技术生产了各种各样的产品投放于化工市场。

关键词：工业应用生物催化剂生物技术化学工业化工市场化合物生产酶

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